1. Relationship between methylation of RSK4 gene and papillary thyroid carcinoma
Yanan YIN ; Jiarui YU ; Jingwei CHI ; Kui CHE ; Zhouyang HUAI ; Yangang WANG ; Fei WANG ; Shihua ZHAO
Chinese Journal of Endocrinology and Metabolism 2019;35(12):1031-1036
Objective:
To detect the methylation status of ribosomal S6 kinase 4 (RSK4)in papillary thyroid carcinoma (PTC)and to study its correlation with mRNA expression and clinical features.
Methods:
134 cases PTC tissues and corresponding paracancerous thyroid tissues were collected. DNA methylation status of RSK4 gene in PTC tissues and corresponding paracancerous tissues were analyzed by methylation specific PCR and bisulfite genomic sequencing, and mRNA expression was detected by quantitative realtime PCR. The relationship of DNA methylation status with mRNA expression and clinical features was analyzed.
Results:
The methylation rate of RSK4 in PTC tissues was significantly higher than that in paracancerous tissues by methylation specific PCR (