OBJECTIVE: To investigate the clinical effect of vancomycin bone cement in the treatment of diabetic foot ulcer (DFU) ruptured Wagner gradeⅡ-Ⅳ. METHODS: From March 2019 to April 2021, 32 patients with Wagner gradeⅡ-Ⅳ diabetic foot were divided into vacuum sealing drainage (VSD) group and bone cement group according to different treatment methods. There were 16 cases in VSD group, 8 males and 8 females;the age ranged from 66 to 81 (70.50±7.20) years, and the course of disease ranged from 8 to 40 (27.56±8.55) months;Wagner gradeⅡin 2 cases, grade Ⅲin 7 cases and grade Ⅳin 7 cases;debridement and VSD were used. There were 16 cases in the bone cement group, 9 males and 7 females;the age ranged from 63 to 79 (69.56±7.29) years, and the course of disease ranged from 11 to 39(22.75±11.43) months;Wagner gradeⅡ in 2 cases, grade Ⅲin 5 cases and grade Ⅳ in 9 cases;vancomycin loaded bone cement was used for treatment. The types of bacteria, negative time of bacterial culture, skin healing time, hospital stay, operation times and complications were observed and compared between two groups. RESULTS: All patients were followed up for 3 to 6 (4.00±1.07) months. The bacterial negative time, skin healing time and hospital stay in bone cement group were significantly lower than those in VSD group ( CONCLUSION Vancomycin loaded bone cement is effective in the treatment of Wagner grade Ⅱ-Ⅳ diabetic foot ulceration wounds. It can reduce the length of hospital stay, shorten the healing time of skin and kill pathogens as soon as possible. It is one of the effective methods to treat Wagner gradeⅡ-Ⅳdiabetic foot ulceration.
Bone Cements/therapeutic use* ; Child ; Child, Preschool ; Diabetes Mellitus ; Diabetic Foot/drug therapy* ; Female ; Humans ; Male ; Treatment Outcome ; Vancomycin ; Wound Healing

Objective To explore the changes in posterior tibial slope after tibial plateau fracture surgery and its relationship with knee joint function.Methods The cases of 83 patients who underwent tibial plateau fracture sur-gery were collected.The posterior tibial slope(PTS)of the patient's unaffected side,the affected side after surgery and the affected side at the last follow-up were measured.He knee joint score data of Lysholm,HSS and Rasmus-sen were evaluated and recorded,and the patients were divided into PTS＜9° group and PTS≥9° group according to the PTS angle after operation.The mean of three kinds of knee joint function scores in the two groups were statis-tically analyzed,and the mean of PTS after operation and final follow-up was also statistically processed.The differ-ence of PTS between the last follow-up and the healthy side was calculated,and the correlation between the differ-ence and the knee joint function data at the last follow-up was analyzed.Results There was no significant differ-ence in general conditions between the two groups of patients.The Lysholm,HSS,and Rasmussen score data of knee joints in PTS＜9° group were significantly higher than those in the PTS ≥9° group(P＜0.05).There was a significant difference in postoperative PTS between the two groups of patients compared to the last follow-up PTS(P＜0.05).The difference between the last follow-up and the PTS of the unaffected side(b)was correlated with Ly-sholm,HSS and Rasmussen knee score data(P＜0.05).Conclusion Postoperative rehabilitation exercise leads to changes in the angle of PTS.The knee joint function of patients in PTS＜9° group is better than that of patients in group PTS ≥9° after surgery.The difference between the last follow-up PTS and the healthy side PTS is correla-ted with knee joint function.Therefore,it is necessary to try to restore the anatomical alignment of the plateau frac-ture during surgery or control postoperative PTS＜9 °.

As a key role in mussel defense system, Mytilin is an important antibacterial peptide isolated from the mussel serum. The structural and functional researches on Mytilin showed that the fragment connecting two beta-sheets in a stable beta-hairpin structure was probably required for antimicrobial activity. To elucidate the structural features and the antimicrobial activity of this fragment, we re-designed and synthesized two peptides corresponding to the main mimic structures of Mytilin-1 from Mytilus coruscus, we named these two peptides Mytilin Derived Peptide-1 and Mytilin Derived Peptide-2, respectively. Using a liquid growth inhibition assay, we evaluated their activity towards Gram-positive, Gram-negative bacteria and fungus. The results showed that both peptides can inhibit the growth of Gram-positive, Gram-negative bacteria and fungus. Besides, these two peptides showed high stability in heat water and human serum. These works laid the foundation for further research on the molecular mechanism of Mytilin and for further exploitation of antibacterial peptides with lower molecular mass and more stable structure.
Amino Acid Sequence ; Animals ; Anti-Infective Agents ; chemical synthesis ; pharmacology ; Antimicrobial Cationic Peptides ; chemical synthesis ; chemistry ; pharmacology ; Molecular Sequence Data ; Mytilus ; chemistry

Hypoxia-inducible factor 1 (HIF-1), as a main transcriptional regulator of metabolic adaptation to changes in the oxygen environment, participates in many physiological and pathological processes in the body, and is closely related to the pathogenesis of many diseases. This review outlines the mechanisms of HIF-1 activation, its signaling pathways, natural inhibitors, and its roles in diseases. This article can provide new insights in the diagnosis and treatment of human diseases, and recent progress on the development of HIF-1 inhibitors.
Disease ; Humans ; Hypoxia-Inducible Factor 1/physiology* ; Oxygen ; Signal Transduction