Objective:To observe the clinical effect of self-made traditional Chinese medicine combined with acupuncture and moxibustion in the treatment of non-small cell lung cancer, and its effect on the serum T cell subsets of patients.Methods:From November 2015 to November 2018, 104 patients with non-small cell lung cancer admitted to Lishui Hospital of Traditional Chinese Medicine were selected in the research.They were divided into observation group and control group according to random number table method, with 52 cases in each group.The control group was treated with arterial infusion chemotherapy, while the observation group was treated with traditional Chinese medicine decoction combined with traditional Chinese medicine acupuncture and moxibustion on the basis of chemotherapy.The clinical symptoms and serum T lymphocyte subsets of the two groups were observed and compared before and after treatment, and the adverse reactions of the two groups were recorded.Results:The symptom scores of the control group and the observation group were (8.49±2.43)points and (8.41±2.21)points, respectively, which were lower than those before treatment[(11.11±2.25)points and (6.23±2.02)points]( t=104.962, 82.738, all P<0.05). The symptom score of the observation group was lower than that of the control group( t=11.638, P<0.05). The incidence of liver function damage in the observation group was 15.38%(6/52), which was significantly lower than that in the control group[34.62%(13/52)](χ 2=5.128, P<0.05). After treatment, the immunologic indicators (CD 3+, CD 4+, CD 8+, CD 4+/CD 8+) in the observation group were (63.24±20.40)%, (35.16±5.02)%, (27.55±13.54)%, (1.28±0.32), respectively, which were significantly improved compared with before treatment[(51.14±15.58)%, (25.64±6.19)%, (36.04±12.65)% and (0.71±0.11)], the differences were statistically significant ( t=18.103, 58.675, 68.789, 19.573, all P<0.05). The improvements of immunological indicators in the observation group were better than those in the control group[(55.84±15.21)%, (28.79±4.23)%, (32.51±9.51)%, (0.89±0.15)], the differences were statistically significant ( t=2.097, 6.997, 2.162, 7.958, all P<0.05). Conclusion:Traditional Chinese medicine decoction combined with acupuncture and moxibustion can effectively improve the clinical symptoms, improve immune function and alleviate adverse reactions of chemotherapy in patients with stage Ⅲ B and Ⅳ non-small cell lung cancer.