Objective To investigate the economic effects in patients with primary hypertension by four kinds of treatment.Methods 160 cases with primary hypertension were randomly divided into Benazepril group(n=40)、Perindopril group(n=40)、Amlodipine group(n=40) and Nitrendipine group(n=40),and data was evaluated with the pharmacoeconomic cost-effective analysis method.Results Hypertensive efficacy in 4 groups were not sig-nificantly different(x2=3.26,P>0.05);The symptom total score before treatment and after treatment was sisnifi-canfly different(a11 P<0.05);The SF-36 Scale after treatment in 4 groups were higher than before treatment(all P<0.05);The costs of pefindopril was lower than the benazepril.Conclusion Pharmacoeconomies played an important role in optimizing therapeutic scheme,guiding rational drug use and increasing economic effectiveness.

Background and purpose:Epithelial-mesenchymal transitions(EMTs) occur as key steps during embryonic morphogenesis,and are now implicated in the progression of primary tumors towards metastases.We have found that the transcription factor Twist,a master regulator of EMT,was expressed markedly in human lung carcinoma cell line(A549).This study is to investigate the effects of short hairpin RNA(shRNA) targeting Twist on EMT and invasion ability of A549 cells in vitro.Methods:RNA interference plasmid that can express short hairpin RNA(shRNA) targeting Twist(positive RNAi plasmid) or express shRNA that does not match any known human coding mRNA(negative RNAi plasmid)was designed,constructed,and transfected into A549 cells line.Positive RNAi A549 cells(Pi group) expressing Twist suppressed or negative RNAi A549 cells(Ni group) expressing Twist uninfluenced was selected by neomycin resistance.In normal(control,C),Pi and Ni group A549 cells,Twist,alpha-smooth muscle actin(?-SMA) and E-cadherin expression were examined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot,invasion ability were examined by Boyden chamber model.Results:1,In C group,both Twist and ?-SMA expressions were strongly positive,but E-cadherin was poorly expressed.The number of A549 cells permeating septum of Boyden chamber(NCS) was equal to 57.4?3.4.2,In Pi group,compared with that in C group,Twist and?-SMA expression,and NCS were all decreased,but E-cadherin expression was significantly increased(P

[Objective]To explore the efficacy and result of femoral component revision by using impaction bone grafting technique with anatomic cemented stem and freeze-dried allograft.[Method]Anatomic cemented stem combined with freeze-dried allograft were used in IBG for 49 cases of femoral revisions from Jan,2001 to Dec 2005.36 cases(73%)of which had severe bone loss(Paprosky grades ⅢB and Ⅳ).The mean follow-up time was 35.3 months(26~52 months).Harris Hip Score System and radiography were used to evaluate the clinical results.[Result]The mean Harris Hip Score was improved from 44.6 points preoperatively to 88.3 points at final evaluation.Good and excellent scores were about 89.8%.No major femoral stem subsidence was found in our patients.There were one case of dislocation(2%),one case of deep infection(2%)and 3 cases of femoral fractures during operations(6.1%),which were not related to the selection of femoral component and the type of allograft.[Conclusion]The impaction bone grafting technique by using freeze-dried allograft and the anatomic cemented femoral prosthesis for femoral revision was feasible and the short and mid-term result was good.Longer follow-up was needed to evaluate the long term result.

Diffuse large B-cell lymphoma(DLBCL),the largest category of lymphoma,is a clinically and biologically heterogeneous disease entity.Immunophenotype,tissue microarray and molecular studies underline the extreme heterogeneity of DLBCL and suggest a subclassification of the tumor.This review summarizes the morphological,immunophenotypic,cytogenetic and molecular genetic features of DLBCL relevant to the precise prognostic previsions.

AIM To study the preparation and stability of sinomenine liposomes. METHODS Taking entrapment efficiency as index, different preparation methods, including thin film dispersion, reverse-phase evaporation, and aether injection were optimized. The leakage rate, the uptake rate and the storage temperature of the liposomes were studied at the same time. RESULTS The entrapment efficiency of reverse-phase evaporation was the lowest as 48.93 % . The entrapment efficiency of thin film dispersion and aether injection were near, as 68.91 T《B》 % and 66.57 % , respectively. CONCLUSION Thin film dispersion has higher entrapment efficiency and low command to the equipment. So it is suitable for the production of sinomenine liposomes on a large scale.

Objective To investigate the value of G6PI in the diagnosis of rheumatoid arthritis(RA).Methods The clinical data of 130 cases of patients with RA treated in the hospital from August 2011 to August 2013 were statistically analyzed.In addition to that,85 non-RA patients with other rheumatic diseases were recruited as non-RA group and 60 healthy people were recruited as control group.Results Serum G6PI,anti-CCP antibodies and RF concentrations of RA group were significantly higher than those of non-RA group and control group(P <0.05),the RF concentrations of non-RA group was significantly higher than those of con-trol group(P <0.05).The positive rates of G6PI,anti-CCP antibodies and RF tests were 67.7%(88/130),52.3%(68/130)and 75.4% (98/130)respectively in RA group,which were significantly higher than those in non-RA and control group(P <0.05),the serum RF concentrations in non-RA group were significantly higher than control group(P <0.05).The sensitivity,negative predic-tive value and Youden index of G6PI were significantly higher than those of anti-CCP and RF(P <0.05).Serum G6PI concentra-tions of RA patients was positively correlated with those of RF(r=0.732,P <0.05).Conclusion G6PI detection can be used in the diagnosis of RA,and is of great value.

Objective To explore the complications and influence of repeat cesarean section after previous cesarean delivery. Methods The styles of the total 67 cases of repeat cesarean section were reviewed, and the mater-nal ages, gestarional weeks, time intervals between the two times of delivery, and the styles of the previous cesarean section were analyzed. Results Of the total 67 case of repeat cesarean section ,there were 8 cases with relatively se-vere adhesion, 1 case with hysterectomy. There were 9 cases with intraoperative blood loss over 500ml(13.4%). The rate of adhesion induced by abdominal transverse incision of cesarean section was significantly higher than that of ab-dominal longitudinal incision of low cesarean section. Conclusions When we determining a style of cesarean section, we should comprehensively consider the obstetrical conditions of the pregnant women to select abdominal incision style and uterine incision style. The quality of previous cesarean delivery determines the complications of repeat cesarean section.

Objective To investigate the effects of 1,25-dihydroxyvitamin D3[1,25(OH)2D3] on regulatory T cells(CD4+CD25+ Foxp3+ T cell)and airway inflammation in asthmatic rats. Methods Asthmatic models were established in 28 rats with ovalbumin as sensitinogen,and four groups were randomly divided(n=7).1,25(OH)2D3 was orally administered 1 h before challenge in treatment group 1,dexamethasone was subcutaneously injected 1 h before challenge in treatment group 2,both 1,25(OH)2D3 and dexamethasone were given 1 h before challenge in treatment group 3,while no medicine was administered 1 h before challenge in group 4(asthmatic control group).The levels of IL-4 and IL-10 in bronchoalveolar lavage fluid(BALF) were detected,the total cell numbers were obtained and the eosinophil count was performed.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells were measured with flow cytometry,and the mRNA expression of IL-10 and Foxp3 in lung tissues was detected.Wistar rats without asthma were served as normal controls(n=7). Results There were significant differences between asthmatic control group and normal control group in each parameter.Compared with asthmatic control group,the total cell number and eosinophil count decreased,the level of IL-4 decreased and the level of IL-10 increased in BALF in treatment groups.The ratios of Treg to CD4+ T cells in peripheral blood and spleen mononuclear cells significantly increased,and the mRNA expression of IL-10 and Foxp3 in lung tissues increased in treatment groups compared with asthmatic control group.There was no significant difference between treatment groups and normal control group in each parameter. Conclusion Administration of 1,25(OH)2D3 increases the proportion of Treg and expression of Foxp3 mRNA in peripheral blood and spleen in asthmatic rats.1,25(OH)2D3 has a regulatory effect on Treg,and may serve as a potential therapeutic approach against asthma.

Objective:To optimize β-cyclodextrin (β-CD) inclusion process for volatile oil in Yingxinning capsules. Methods:The content of volatile oil in theβ-CD inclusion compound as the evaluation index, the ratio of volatile oil toβ-CD, inclusion tempera-ture, inclusion time and stirring speed as the influencing factors, the inclusion process was studied using L9(34)orthogonal design. Re-sults:The best preparation conditions were as follows:the ratio of volatile oil toβ-CD was 1∶ 9, the inclusion temperature was 40℃, the inclusion time was 30min and the stirring speed was 800 r·min-1 . Conclusion: The preparation technology is simple, feasible and stable with high inclusion rate of volatile oil.

Objective Experimental and clinical data suggest that fixed element knee designs a re not able to both supply adequate mobility and eliminate unnecessary constra int forces,as well as provide low contact stresses compatible with pr olonged life of UHMWPE bearing components.The present study investi-gated the preliminary clinical outc ome of TACK(Total Articulating Cementless Knee)system,a new design of mobile-bearing knee prosthesis.Methods Eighty-eight knees in 61patients underwent total knee replace-ment by using TACK were reviewed.Forty-five knees of 31patients were followed-up at least one year.The HSS knee rating score system as well a s X-ray films were used to evaluate th e clinical results with the mean follow-up period of 25.4months.Results At the latest follow-up examination,the rate of good to excellent clinical results was 96.4%.The range of motion was improved from 0-6.5-68.3degrees preoperatively to0-2-88.4degrees at the latest follo w-up.The complications such as oste olysis around the prosthesis,prosthetic loosening and dislocation of meniscus bearings were not found.Conclusion The TACK Knee is a third generation knee system design ed to introduce movable bearings between the metal tibial and femoral components that would reduce the sur face and subsurface stress states at the bearing surfaces and at the bone-implant interfaces by maximiz ing the conformity of the tibial and f emoral components and allowing mo-bility of the bearing surface.In an a verage of more than two years experie nce,it was observed that there was no aseptic loosening of the componen ts.A long term follow-up is obviously necessary to make more precise statement about this knee replaceme nt system.