Objective To investigate costimulatory molecules CD80 and CD86 expression in acute myelogenous leukemic cells and clinical implication.MethodsThe expression of CD80 and CD86 in the patients with acute myelogenous leukemia and HL-60 cells,U937 cells,NB4 cells,K567 cells was confirmed by Flow Cytometer.ResultsCD80 was very low or no expression in patients with acute myelogenous leukemia.CD86 expressed in acute myelogenous leukemia (27.86±19.65)%,which was much higher than that in control group[(1.21±0.13)%,t＝3.55,P＜0.01].No significant changes were observed in the expression of CD86 in M4 cells(48.65±21.92)%,M5 cells(39.25±18.67)% and control group(50.20±20.31)%(P＞0.05).After the cells were cultured for 24 h and 48 h,the expression of CD86 was (30.62±5.35)% and (29.43±4.67)% in HL-60 cells ,(24.12±5.23)% and (26.56±6.54)% in U937 cells,and,(21.25±3.78)% and (23.21±6.98)% in NB4 cells (all P＞0.05).The expression of CD80 and CD86 was very low in K562 cells.ConclusionsCostimulatory molecules CD86 expressed in acute myelogenous leukemic cells in the patients with acute myelogenous leukemia and HL-60 cells,U937 cells,NB4 cells.

ObjectiveTo evaluate prognostic factors and the surgical results of pulmonary carcinoid tumors.Methods We retrospectively reviewed the medical records of 62 patients who were diagnosed as pulmonary carcinoid tumors between January 2000 and October 2010 at Department of Thoracic Surgery,Shanghai Chest Hospital.The following information was available for each of the 62 patients:age,sex,pathological type,and TNM stage.ResultsThere were no operative death.The 3-year and 5-year survival rates after surgery were 92.1％ and 77.8％,respectively.Of the 62 patients,42 were diagnosed as typical carcinoid tumor,and among them,4 patients (8.3％) had lymph node metastases.Their 3-year and 5-year survival rates were 97.8％ and 94.7％,respectively.The remaining 20 patients were diagnosed as atypical carcinoid tumor,and among them,6 patients (37.5％) had lymph node metastases.Their 3-year and 5-year survival rates were 84.4％ and 58.8％,which were statistically significant compared with typical carcinoid tumor( P =0.0047 ).There was significant difference in survival rate between the patients with lymph node metastases and the patients without lymph node metastases (P =0.0048).CondusionThe main risk factors affecting survival rate of those patients who were diagnosed as pulmonary carcinoid tumors were pathological types and lymph node metastases.

Objective To describe the clinical and imaging manifestations of patients with synovitis acne pustulosis hyperostosis osteomyelitis (SAPHO) syndrome,and to analyze the diagnostic importance of different clinical and imaging manifestations for SAPHO syndrome. Methods Seventeen patients (7 males and 10 females) with SAPHO syndrome were recruited in this study.Age ranged from 36 to 67 years with a mean age of (48 ± 8) years. All patients fulfilled the diagnostic criteria of Benhamou. Serum HLA B27 antigen records were reviewed for all patients. Imaging data of the abnormal bone sites were collected by conventional radiograph in all patients,CT in 13 patients as well as MR in 3 patients.Average time to take for a definite diagnosis of the syndrome was 3.7 years (ranged from O.5 to 13 years).Results Serum HLA B27 antigen was positive in all patients. Both skin and bone abnornalities were found in all patients.Ten patients had skin palmoplantar pustulosis and two patients had acne. Involving sites of bone and joints include sacroiliac joints,anterior chest and limbs.Sacroiliac joints were asymmetrically involved with imaging features in all patients.Eight patients exhibited anterior chest wall involvement. Five patients had osteomyelitis at limbs. For all images of 17 patients,CT was superior to conventional radiography in detecting abnormal changes of bone erosion and soft tissue swelling.MR imaging was able to depict edema changes that was not detectable by CT and radiography.Conclusion SAPHO syndrome is a rare disease,but for patients with skin and bone-joint abnormalities,especially with skin palmoplantar pustulosis,acne as well as with imaging features at the sacroiliac joint and anterior chest wall,SAPHO syndrome should be taken into a diagnostic consideration.

Objective To explore the influence of levothyroxine(L-T4) in the treatment of primary hypothyroidism on myocardial enzymes and lipids.Methods 78 patients with primary hypothyroidism were selected and treated with L-T4 for 12 weeks.The fasting serum levels promote thyroid hormone(TSH),free T3 (FT3),free T4 (FT4),total cholesterol (TC),triglycerides (TG),low-density lipoprotein cholesterol (LDL-C),aspartate aminotransferase enzyme (AST),creatine kinase (CK) and its isoenzyme (CK-MB),lactate dehydrogenase(LDH) were monitored and analyzed before and after treatment.Results After L-T4 treatment for 12 weeks,compared with before treatment,TSH,TC,TG,LDL-C,CK,CK-MB,LDH,AST were significantly decreased or restored (t =10.5223,26.8498,22.7699,16.2735,22.9329,13.1910,32.0907,22.9597,all P ＜ 0.01).The FT3 was negatively correlated with TG (r =-0.3782),LDL-C(r =-0.3506),AST(r =-0.2843),LDH(r =-0.2974),CK(r =-0.3726) (all P ＜ 0.01)and CK-MB(r =-0.2559) (P ＜ 0.05).FT4 was negatively correlated with TC (r =-0.2660),TG (r =-0.4661),LDL-C(r=-0.5119),LDH(r=-0.5936),CK(r=-0.4877),CK-MB(r=-0.5463) (all P＜0.01)and AST(r =-0.2328) (P ＜0.05).TSH was positively correlated with TC(r =0.5341),TG(r =0.7567),LDL-C(r =0.8240),AST (r =0.3923),LDH (r =0.8073),CK (r =0.9661),CK-MB (r =0.7336) (all P ＜0.01).TSH had,the best correlationship with CK (r =0.9661).Conclusion L-T4 can significantly improve the thyroid function and reduce the blood lipids,myocardial enzymes levels of patients with primary hypothyroidism.

Anal Canal ; surgery ; Child ; Child, Preschool ; Colon, Sigmoid ; surgery ; Digestive System Surgical Procedures ; methods ; Female ; Hirschsprung Disease ; surgery ; Humans ; Infant ; Laparoscopy ; Male ; Rectum ; surgery ; Treatment Outcome

Adult ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Radiation, Ionizing ; Radiology Department, Hospital ; Serum ; metabolism ; radiation effects ; Testosterone ; blood ; Thyroid Hormones ; blood

Objective To establish the quality control standard for Shuanggen Qingnao Granules.Methods TLC was used to identify Yiyiren SEMENCOICIS,Yujin RADIXCURCUMAE,Banlangen RADIX ISATIDIS. HPLC was used to determine the content of Puerarin in Shuanggen Qingnao Granules.Results The TLC spots developed were fairly clear,and the bland test showed no interference.Puerarin showed a good linear relationship in the range of 5.184～40.50?g/mL,the average recovery of Puerarin was 99.93%,and RSD=1.81%.Conclusion The method is accurate and quick,and can be used for the quality control of Shuanggen Qingnao Granules.

Objective To investigate the iodine nutritional status in the populations of Guizhou Province after implementation of a new standard of salt iodine and to provide a basis in formulating strategy for control of iodine deficiency disorders.Methods In 2011 and 2014,30 units were sampled in Guizhou Province using the sampling method of Probability Proportional to Size.One primary school was chosen in each unit,by the randomized sampling method,40 or 50 students in each school were sampled for examining their urinary iodine level and for testing their household salt iodine level;12 and 50 children were chosen before and after the adjustment,respectively,for testing their urinary iodine level;near the location of the primary school,1 township was chosen,15-20 pregnant women in each township were sampled to test their urinary iodine level.Results Totally 1215 salt samples were collected before adjustment,the median of salt iodine was 32.2 mg/kg;consumption rate of qualified salt was 96.63％;totally 364 urine samples of 8-10 children were collected,the median of urinary iodine (MUI)of children was 308.9 μg/L;450 urine samples of pregnant women were sampled,and their MUI was 216.5 μg/L,among these,121 below 150 μg/L,the proportion was 26.9％.Totally 1 538 salt samples were collected after the adjustment,the median of salt iodine was 26.6 mg/kg;consumption rate of qualified salt was 96.40％;totally 1538urinary samples of 8-10 children were collected,the MUI of children was 218.3 μg/L;and 601 urinary samples of pregnant women were collected,the MUI was 175.1μg/L,among these,241 below 150 μg/L,the proportion was 40.1％.As can be seen from the results,the percentage of sample below 150 μg/L after the adjustment was increased to 13.2％ compared to that before the adjustment,the difference was statistically significant (x2 =19.89,P ＜0.01).Conclusion After implementation of a new standard of salt iodine in Guizhou Province,the iodine nutrition is suitable in residents,but the iodine nutrition of pregnant women is in a trend of declining,which should be noticed.

Objective To investigate the expression of miR-30d in breast cancer tissues and demonstrate the regula-tive effects of miR-30d ASO on the invasion and migration of breast cancer cells in vitro. Methods The expression levels of miR-30d in 108 breast cancer tissues and their adjacent tissues were detected by real-time quantitative PCR method. Af-ter transfection with miR-30d ASO, the biological effects of miR-30d on in breast cancer cells was measured by transwell as-say and wound healing assay. The expressions of matrix metalloproteinase (MMP)-2 and MMP-9 were analyzed by Western blot assay. Results The expression level of miR-30d was found to be over-expressed in breast cancer tissues(P<0.05). Compared with control group and nonsense interference group, the miR-30d expression was significantly decreased in breast cancer cells(transfection with miR-30d ASO). Results of transwell and wound healing assay showed that the invasion and mi-gration ability decreased significantly after transfection with miR-30d ASO, and expressions of MMP-2 and MMP-9 were down-regulated (P<0.05). Conclusion miR-30d was over-expressed in human breast cancer. The invasion and migration of breast cancer cells can be effectively inhibited by decreasing the expression of miR-30d. miR-30d may become a new tar-get for the regulation of invasion and migration in breast cancer.

BACKGROUND:In the field of orthodontics, molar bands play an important role. But traditional bands have some weakness difficult to be solved in clinical use, for example, trying on cumbersomely, having to separate the
adjacent teeth beforehand, occupying bigger space and tending to cause periodontitis of molars with bands. So some clinicians use a buccal tube instead of the molar band, but it has relatively high losing rate and cannot be fixed with Transpalatal Arch and Nance.
OBJECTIVE: To invent a new open “C”-shaped molar band with nanomaterial (patent number: ZL201110057699.1) to solve the above problems as a common molar appliance.
METHODS: We made some stainless steel strips of 0.18-0.20 mm thickness and a certain length. The stainless steel strips were made to the new bands composed of buccal, lingal, distal surface and bilateral extention caused by removable parts extending to mesial surface. In order to improve retention force, its inner surface was coated by nano-hydroxyapatite and it bound to the molar crown by resin reinforced glass ionomer cements in clinical use. RESULTS AND CONCLUSION: The new open “C”-shaped molar band with nanomaterial overcomes
shortcomings of conventional band and buccal tube. From the design, in contrast with traditional band, it not only has no need to separate teeth beforehand and to try on repeatedly, but also less stimulates periodontal tissues. So the new band can be more suitable for clinical use.