OBJECTIVE:To investigate the inhibition effect of berberine on colon carcinoma lovo cell. METHODS:Colon car-cinoma lovo cells were cultured and treated with berberine and meloxicam in various concentrations. At 6 h,12 h and 24 h after treatment,the proliferation of lovo cell was assayed by MTT method. RT-PCR method and Western blot testing were used to eval-uate the mRNA and protein expressions of COX-2 in lovo cell. RESULTS:Berberine with concentration larger than 10-5 mol?L-1 and meloxicam with concentration larger than 10-4 mol?L-1 could inhibit the growth and proliferation of lovo cell and showed concentration and time dependent manners(P

Objective To study the effect of the Hushengujingfang (HSGJF), a traditional Chinese prescription, on the biological behaviour of rat mesangial cell (MC). Methods MC was cultured in vitro, and the serologic pharmacology method was employed. The cells were divided into serum groups of HSGJF, LPS group and control group. Added the different concentration actors to MC and cocultured then made into unicell suspension. After examining it by flow cytometry, the percentage of G 0 /G 1 period, S period, G 2 /M period in cell cycle and the inhibition percentage of S period and cell proliferation index were calculated. Result The actors of different concentration had different effects to the multiplication cycle of MC and delayed the entry of cell cycle at G 0 /G 1 period. HSGJF induced apoptosis of MC. Conclusion One of action mechanism of HSGJF treating mesangial proliferative nephritis and other renal glomerular diseases is to inhibit MC proliferation and promote apoptosis.

0.05].Conclusion To the middle-and old-aged GI cancer patients without organ dysfunction,lowering blood transfusion trigger from Hb 100 g/L to Hb 80 g/L during operation can meet the requirement of oxygen,reduce blood transfusion,and do not increase the rates of wound infection or delay wound healing.

Whether the sentinel lymph node (SLN) metastasis has important clinical significance for the therapy and prognosis of tumor. Sentinel lymph node biopsy (SLNB) has became the most accurate clinical method to confirm the status of sentinel lymph node. And the key of the success of SLNB is the localization of SLN. The methods used to locate SLN mainly are blue dye tracer method, radionuclide tracer technique, blue dye tracer method combined with radionuclide tracer technique, indirect lymphography, NIR imaging, and photoacoustic imaging. This article demonstrates the principle, application method and contrast agents of the indirect lymphography and the application in head and neck tumor.
Contrast Media ; Head and Neck Neoplasms ; diagnosis ; Humans ; Lymph Nodes ; Lymphatic Metastasis ; Lymphography ; Prognosis ; Sentinel Lymph Node Biopsy

Objective To study the sub chronic toxicity of silver nanoparticles on medaka.Methods Adult fish were divided into silve nenopartides and control group.Animals were collected on 14 days after exposure, and some toxicological endpoints such as death rate, tissue distributed of silver irons, oxidative stress and histopathological damage were measured.Results There were significant difference in death rate of medaka treated with silver nanoparticles and control group.Compared with the control group, the content of silver in gill, intestine and liver of medaka treated with silver nanoparticles were increased significantly.Compared with control group, the activity of LDH in liver and SOD in liver and gill were significantly decreased(P<0.01).The content of MDA in liver of medaka treated with silver nanoparticles was significantly increased(P<0.01).The liver and gill of mekada treated with silver nanoparticles were damaged, compared with control group.Conclusion Nano silver has a certain subchronic toxicity to aquatic life.

Objective: To explore the heat injury level of vocal cord by different power of the Diomed 25 semi conductor laser so as to provide scientific evidence for clinical laser therapy of larynx diseases. Methods: Canine was used. The power of laser was set on 5, 10 and 20 W respectively, and the time of laser exposure was fixed on 2 s.When canine vocal cord was hit by laser in vivo , the depth and width of the tissue heat injury were measured. Results: When laser hit with 5, 10 and 20 W for 2 s exposure time, the corresponding depth of the tissue heat injury were 0.2 0.4,0.4 0.6,0.8 1.0 mm respectively, the corresponding width of the tissue heat injury were 1.0 1.7,1.7 2.0,2.0 2.6 mm respectively. There was significant difference between the tissue heat injury levels caused by different laser power under the same exposure time( P

Ubiquitin-proteasome pathway is one of the primary pathway in intracellular protein degrada-tion,therefore the ubiquitin conjugating enzyme D3(UbE2D3)which involved in the ubiquitin mineralization process can affect the biological effects accordingly to affect some protein and nucleic acid content and activity. The function that participate in modification and degradation of some cancer factors is vital in tumor cells,and followed by tumor biological behavior changing. Researches show that UbE2D3 correlates with human telomer-ase reverse transcriptase( hTERT),radiation sensitivity,aggressive,etc in breast cancer.

Objective To investigate the clinical value of homocysteine (Hcy) ,cystatin C (Cys C) and high‐sensitivity C‐reactive protein (hs‐CRP) in atherosclerosis (AS) .Methods Totally 100 cases of newly diagnosed patients of AS were choosed as the ob‐servation group ,36 cases condition improved markedly of AS after treatment as the treatment group ,and 120 cases of normal medi‐cal groups as the control group ,the Hcy ,Cys C and hs‐CRP in all subjects were detected respectively .Results Hcy ,Cys C and hs‐CRP levels of observation group were significantly higher than those of normal groups ,and the difference was statistically signifi‐cant (P< 0 .05) .While the Cys C and hs‐CRP levels of treatment group were significantly lower than the those of observation group ,the difference was also statistically significant (P< 0 .05) .Conclusion The detection of Serum Hcy ,Cys C and hs‐CRP might be help to the diagnosis ,treatment evaluation and prognosis of AS .

Objective: To study the pathophysiological role of tumor necrosis factor ? (TNF ?) and interleukin6 (IL 6) in trauma by assaying concentration of TNF ? in blood after maxillofacial gunshot wounds in dogs. Methods: Before and after gunshot wounds, concentration of TNF ? and IL 6 in the plasma of dogs were assessed by ELISA method at different times. Results: Although the systemic injuries in our gunshot wound model were no very severe and the dogs had no severe complications, the TNF ? concentration in plasma became significantly higher in the first 2 6 h after the gunshot wounds. They varied greatly with each dog and became normal after 10 h without treatment. IL 6 was not detected in blood. Conclusion: TNF ? expression in blood after gunshot wounds is the normal host immune response to the trauma, its circulating concentrations might be associated with the injury severity. But their transient expressions do not significantly increase the severity of the trauma. [