Drug Resistance, Viral ; Endopeptidases ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; virology ; Humans ; Interferons ; pharmacology

Ceramides ; Fatty Liver, Alcoholic ; Humans

Objective To investigate the protective effects of ulinastatin on the hearts of rats with anoxia-induced cardiac arrest-cardiopulmonary resuscitation(CA-CPR) and the mechanism of improving cardiac dysfunction .Methods Twenty male Sprague Dawley(SD) rats were randomly divided into three experimental groups :sham operation group (group A ,n= 8 ,only anesthesia , tracheotomy tube and vascular puncture) ,control group(group B ,n= 6 ,normal saline 4 mL · kg -1 injected via vein) ,Ulinastatin treatment group(group C ,n=6 ulinastatin 50 000 U/kg+normal saline 3 mL · kg -1 injected via vein);Factors including mean arte-rial pressure(MAP) ,left ventricular end diastolic pressure(LVEDP) ,the maximum rising and falling rates of left ventricular deep pressure(± LVdp .dt-1max) ,brain natriuretic peptide(BNP) ,cardiac troponin T(cTNT) ,IL-12 and TNF-αwere observed at setting time before and after cardiopulmonary resuscitation in rats .Results Compared with those of the group A and before CA-CPR ,the concentrations of IL-12、cTNT、TNF-α、BNP、and LVEDP increased(P<0 .01)while ± LVdp .dt-1max decreased(P<0 .01) at 6 h after CA-CPR in group B ,C .Compared with those of group B ,the concentrations of IL-12、CTNT、TNF-α、BNP and LVEDP of 6 h after CA-CPR in group C were lower and ± LVdp .dt-1max was higher(P<0 .01) ,The concentrations of MAP of 6 h after CA-CPR in group B was lower Compared with that of group A ,C and before CA-CPR(P<0 .01) .Conclusion Ulinastatin can improve cardiac dysfunction by depressing mediators of inflammation and reducing myocardial injury .

Objective To explore the value of endoscopic retrograde cholangiopancreatography (ERCP)in the treatment of chronic pancre-atitis (CP).Methods Thirty patients with CP who underwent ERCP from January 2008 to December 2012 were retrospectively analyzed. Serum amylase levels were determined before and at 24 hours after surgery.The reduction in abdominal pain was dynamically evaluated be-fore and after treatment.A follow -up of patients with steatorrhea and anxiety was performed.Regular reexamination using abdominal ima-ging or ERCP was performed to identify pancreatic lesions and conditions of pancreatic duct stents.Results All patients were successfully treated by ERCP and had pancreatic duct stents placed.The remission rate of abdominal pain reached 83.3% at 72 hours after surgery.One patient was assigned to surgery due to insignificant improvement in symptoms,and the case -fatality rate after surgery was 0.Conclusion ERCP is an effective method in the treatment of CP,which has the advantages of being safe,effective,and minimally invasive.

Objective To investigate the correlation between regional cerebral oxygen saturation (rSO2) value and the prognosis of comatose patients in the intensive care unit (ICU).Methods From January 2013 to September 2014, a total of 64 comatose patients admitted in the department of ICU were enrolled.The patients were divided into two groups : consciousness group (n =25) and coma group (n =39).The level of the Glasgow coma scale (GCS), APACHE Ⅱ score and Full Outline of UnResponsiveness score (FOUR) of patients were monitored.The rSO2 was measured by Somanetics INVOS 5100 monitor.Results The differences in levels of FOUR and GCS at admission and GCS at discharge between the two groups were statistically significant (P ＜ 0.05).In the consciousness group, the levels of GCS、 FOUR and rSO2 were higher compared with the coma group (P ＜ 0.05).ROC curve analysis revealed that the areas under the curve of GCS, FOUR and rSO2 for predicting prognosis were 0.823 (0.718-0.928), 0.820 (0.728-0.912) and 0.924 (0.863-0.985) respectively.Conclusions The rSO2 was useful for estimating the prognosis of comatose patients in general ICU.

The serum level of intravenously dripped phenytoin was deter- mined in rabbits before and in different time intervals of a week after intramuscular injection of diazepam with fluorescence polarization immunoassay and the parameters were calculated on a microcomputer.It was found that in the 12th hour after diazepam injection,the serum level of phenytoin was significantly different from that of the control,and t1/2 of phenytoin was significantly prolonged;the model of pharmacokinetics of phenytoin was changed from one compartment open model to two compartment open model.Yet the AUC and Cl of phenytoin were not markedly altered.

Objective To evaluate the effect of pantoprazole and norepinephrine injection on serum levels of inflammatory factors and hemostasis in the treatment of patients with peptic ulcer bleeding .Methods 102 cases of patients with peptic ulcer bleeding from September 2014 to September 2016 in our hospital were selected and randomly divided into observation group ( n =51 ) and control group ( n =51 ) .Observation group were received pantoprazole and norepinephrine injection combination therapy, the control group received only pantoprazole therapy.The treatment lasted 3 days.The efficacy, bleeding time, hospitalization and blood transfusion, serum IL-6 and IL-10 levels, the incidence of rebleeding and incidence of adverse reactions were compared between two groups.Results The overall response rate ( 98.02%) was significantly higher ( 82.35%) ( P<0.05 );observation group bleeding time was significantly faster than the control group ( P<0.05 ) , length of hospital stay was significantly shorter than the control group (P<0.05), blood transfusion was significantly less than the control group (P<0.05); levels of serum IL-6, IL-10 after treatment were lower than those before treatment (P<0.05); the serum levels of IL-6, IL-10 after treatment were significantly lower than those in control group (P<0.05); rebleeding rate in observation group was significantly lower than that in control group (P<0.05);there was no significant difference in adverse reactions between two groups.Conclusion Pantoprazole and norepinephrine injection in the treatment of digestive bleeding ulcer bleeding has the obvious effect, can reduce serum levels of inflammatory factors, while also reduce the incidence of rebleeding, safe and reliable.

Objective To evaluate the safety of nasogastric tube feeding and nasojejunal tube feeding in early enteral nutrition treatment of acute pancreatitis.Methods Using key words,subject headings,and citation tracing,we searched literatures reporting randomised controlled trials on early enteral nutrition treatment of acute pancreatitis through nasojejunal tube and nasogastric tube in the following databases:PubMed,Embase,Cochrane library,Wanfang,China National Knowledge Infrastructure (CNKI),and VIP published since the founding of the databases up to 2016.Meta-analysis was performed with the selected literature.Results Seven randomised controlled trials with 367 patients were included.Meta-analysis showed that the nasogastric tube group was not inferior to the nasojejunal tube group in the incidence of recurrent abdominal pain,gastrointestinal adverse reaction,the total length of hospital stay,and mortality.Conclusion Enteral nutrition via nasogastric tube is safe and well tolerated,may be a safe approach of nutrition treatment for acute pancreatitis.

Objective To investigate IL-18 expression of monocyte cells and the inhibition effect of fluvastation on its expression in patients with acute coronary syndrome(ACS). Methods Isolated monocyte cells from peripheral blood of patients with ACS were incubated with different concentrations of fluvastatin, and IL-18 mRNA level of the monocyte cells was detected by reverse transcription polymerase chain reaction(RT-PCR). Results The monocyte cells of peripheral blood in patients with ACS expressed IL-18 mRNA. IL-18 mRNA decreased from 54 1?9 9 to 50 1?12 6(P

Background and purpose: Since the detection of localized prostate cancer is increasing, it's important to distinguish from benign lesions like prostatitis. This study aimed to compare diffusion weighted imaging with apparent diffusion coefifcients in differential diagnosis of localized prostate cancer on 3.0T MR. Methods:Sixty-nine cases with localized prostate cancer proved by pathology, 43 in perpheral zone (PZ) and 26 in central gland (CG), 33 with prostatitis, and 37 with benign prostatic hyperplasia (BPH) were analyzed. The signal noise ratio (SNR) and apparent diffusion coefifcient (ADC) value of lesions were measured, and a semiquantitative grading of DW image was performed. The diagnostic accuracy of both methods was evaluated by ROC. Results:45 cancer foci and 36 prostatitis lesions in PZ, 27 cancer foci and 42 BPH lesions in CG were included. The sensitivity and speciifcity for ADC value to distinguish cancer from begin lesions in PZ and CG were 88.9%and 86.1%、81.5%and 73.8%respectively. The diagnostic accuracy of ADC value was higher than DWI semiquantitative grading and SNR (P<0.05). Conclusion:ADC value yielded a higher accuracy in differential diagnosis of localized prostate cancer on 3.0T MR, thus it’s recommended as a major index for diagnosis.