Objective To observe the effect of anxiety and depression on cell adhesion molecules and fibrolytic activity in cardiac-pacing patients. Methods The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 were measured in 39 cardiac-pacing patients with anxiety or depression as rersearch group, 32 cases cardiac-pacing patients without any psychological problems as observation control group and 20 healthy subjects as normal control group. Result The levels of plasma P-selectin, E-selectin, sICAM-1, vWF and PAI-1 in cardiac-pacing patients were significantly higher than those in normal control group, and their plasma levels in the patients with anxiety or depression were significantly higher than those in the patients without psychological problems. Conclusion There were significantly elevated cell adhesion molecules level and decreased fibrolytic activity in cardiac-pacing patients. The anxety and depression status could aggregate this condition.

Objective To explore the diagnosis and treatment of abdominal cocoon. Methods This article retrospectively summarized clinical data of 6 cases of abdominal cocoon. Results All the 6 cases of abdominal cocoon were misdiagnosed preoperatively: 5 cases were diagnosed as having intestinal obstruction and 1 case,abdominal mass.Partial or total capsule resection was adopted in all the 6 cases,1 of which simultaneously underwent intestinal arrangement. Conclusions Abdominal cocoon is not specific,which is difficult to diagnose preoperatively.Upper digestive tract radiography,B-ultrasonography and CT scanning help to make the diagnosis.Partial or total capsule resection,intestinal arrangement,intestinal resection and release for intestinal adhesion are usually adopted as the treatment.

Objective To investigate the relationship between C-peptide and artherosclerosis in lower ex-tremity in patients with type 2 diabetes. Methods Two hundred and fourteen type 2 diabetic patients were included in this retrospective study. They were divided into two groups:type 2 diabetes with artherosclerosis in lower extremi-ty in 123 cases and type 2 diabetes without artherosclerosis in lower extremity in 91 cases. Their medical history and the laboratory data were collected such as fasting serum C-peptide levels and postprandial serum C-peptide levels of 1 hour, 2 hours and 3 hours, glycated hemoglobin (HbA1c) and so on. Results Compared with artherosclerosis group in lower extremity, the age, duration of diabetes, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), glycated hemoglobin and smoking rate were significantly higher in type 2 diabetes without artherosclero-sis in lower extremity (P < 0.05). However, the high density lipoprotein cholesterol (HDL-C) was much lower in the last group (P<0.05). There were no significant differences in the other indicators between the two groups (P>0.05). Logistic regression analysis showed that fasting and postprandial (1 hour, 2 hours and 3 hours)serum C-pep-tide levels were all negatively related to artherosclerosis in lower extremity (OR 0.524,P = 0; OR 0.440, P = 0;OR 0.688, P=0;OR 0.795, P=0). Conclusions Serum C-peptide level may be an independent associated fac-tor with artherosclerosis in lower extremity in type 2 diabetes.

Objective To systematically evaluate the association between lactotransferrin(LTF) gene rs1126478 polymor‐phism and caries susceptibility .Methods The published literatures on the association between LTF gene rs1126478 polymorphism and caries susceptibility were retrived from the electronic databases of Pubmed ,Web of science ,Embase ,EBSCO ,Springerlink , CNKI ,VIP ,Wanfang and CBM by computer .The pooled odds ratios (ORs) and its 95% confidence intervals (95% CIs) were used as the effect indicators .The statistical analysis was performed by using the RevMan 5 .2 and Stata 12 .0 softwares .The bias evalua‐tion and sensitivity analysis were also performed .Results A total of 5 case‐control studies involving 720 cases and 412 controls were included .The meta analysis results showed that no statistical significance in the association between LTF gene rs1126478 pol‐ymorphism and caries susceptibility was revealed in all four genetic models (GG+ AG vs .AA :P= 0 .75 ,OR= 0 .93 ,95% CI=0 .59-1 .46 ;G vs .A :P=0 .88 ,OR=0 .97 ,95% CI=0 .68 -1 .38 ;GG vs .AA :P=0 .84 ,OR=1 .07 ,95% CI =0 .57 -1 .99 ;GG vs .AG+AA :P=0 .52 ,OR=1 .12 ,95% CI = 0 .79-1 .58) .Similarly ,the subgroup analysis by ethnicity showed no statistical sig‐nificance in the onset risk between Caucasian and Asian populations as well (both P>0 .05) .Conclusion The LTF gene rs1126478 polymorphism may have no relation with the susceptibility to caries .

Objective To explore the genotype distribution of methylenetetrahydrofolate reductase ( MTH-FR)C677T,A1298C and methionine synthase reductase (MTRR) A66G involved in the folic acid biosynthetic path-way among Chinese Han gestational age women in Sanhe City .Methods 601 samples were recruited from Sanhe re-gion,genomic DNA was obtained from the oral mucosa cells .The detections of MTHFR and MTRR gene polymor-phisms conducted with Taqman -MGB technology .The distribution of gene polymorphisms of this study was analyzed and compared with partial regions of China ,which were reported.Results The frequency of MTHFR 677TT among Sanhe women(37.40%) was significantly different to Yanbian (28.30%),Zhenjiang(21.84%),Songzi(15.40%), Deyang(13.80%),Huizhou(10.90%),Qionghai(6.14%),Zibo(43.6%) (χ2 =12.60,87.44,151.95,233.02, 61.87,446.90,7.27,all P<0.05).The frequency of MTHFR 1298CC(2.30%) was significantly different to Zibo (1.44%),Zhenjiang(3.50%),Songzi(2.60%),Deyang(6.26%),Huizhou(7.20%),Qionghai(7.13%) (χ2 =5.84,6.49,14.32,32.54,22.94,53.12,all P<0.05).The frequency of MTRR 66GG (7.50%) was significantly different to Qionghai (9.25%),Songzi(6.40%)(χ2 =16.34,4.10,all P<0.05).Conclusion The MTHFR, MTRR polymorphism distribution of Han women in Sanhe City is region specific ,respective .

AIM: To explore the effect and mechanism of preoperative transarterial chemoembolization on nephroblastoma.METHODS: Comparative analysis of clinical and pathological features in 39 children with Wilms’ tumor was conducted. TUNEL assay was used to detect the apoptosis of tumor in two groups with or without preoperative interventional treatment. The expressions of P53, Bcl-2 and Bax proteins were detected by immunochemical methods. The patients were followed-up for more than 2 years.RESULTS: The extent of neoplastic cell necrosis and degeneration, interstital fiber tissue hyperplasia of tumor and the number of infiltrating lymphocytes were observed, which were higher in interventional group than those in simple excision group (P

Objective To explore whether the polymorphism of human frizzled 3 (rs2241802 ,rs2323019,rs352203) is associated with schizophrenia in Yunnan Han population. Methods PCR-RELP was used to detect the polymorphism of human frizzled 3 (rs2241802 ,rs2323019, rs352203 ) in 153 patients and 100 controls. All patients were evaluated with positive and negative symptom scale(PANSS). Following a linkage disequilibrium test in the 3 loci ,association analysis was carried out between the polymorphism, including haplotype,and schizophrenia in all samples. Results No significant difference of genotype and allele frequency of the 3 loci were observed in 2 groups. There was no difference in genotype and allele frequency of rs2241802 ,rs2323019,rs352203 in Han samples (P＞0.05) . The polymorphisms of the 3 loci had been observed no difference in case-sibling control study of normal group. A-A-C haplotype and G-A-C haplotype were observed with a higher frequency in both samples (P＜0.05). There was no difference in patients and controls in PANSS scores (P ＞ 0.05 ). Conclusions There is no association between polymorphisms of human frizzled 3 ( rs2241802, rs2323019, rs352203 ) and schizophrenia in Han. A-A-C and G-A-C haplotype might possibly associate with schizophrenia in Yunnan Han population.

Mitochondrial oxidative stress has been recognized as a preliminary and critical factor that aggravates the pathological cascade of Alzheimer's disease, which induces the production of β-amyloid protein, upregulates the expression of phosphorylated tau protein and triggers oxidative damage to lipids, proteins and mitochondrial deoxyribonucleic acid. Central neurons are more vulnerable to oxidative stress than non-neuronal cells due to their high oxygen demand, abundant unsaturated fatty acids and antioxidant enzymes deficiency. On this account, this review introduces the causes of mitochondrial oxidative stress, and analyzes the important role of mitochondrial oxidative stress in the pathogenesis of Alzheimer's disease. Meanwhile, the review focuses on the design and intervention strategies of drug delivery systems targeting mitochondrial oxidative stress in neurons, aiming to provide new ideas for the prevention and treatment of Alzheimer's disease.

10.3969/j.issn.1000-3606.2013.06.003

AIM:To explore the regulation of lumbar physiological curvature by angulation traction and longitudinal rotation and the clinical significance of repairing spinal balance on lumbar disc herniation(LDH). METHODS:①Totally 71 patients with LDH diagnosed by CT at the 451 Hospital of Chinese PLA were enrolled from October 2005 to January 2006. Three-dimensional swirl angle program control restitution bed was applied. This bed was composed of fixing chest swirl section,magnetic heat lumbar flexure massage section and traction haunch angulation section with the pull strength of 20-90 kg. ②The patients were divided into experimental group(n =38) and control group(n =33). ③All patients of the two groups received supine genuflex traction for 20 minutes and combined with longitudinal rotation for 10 minutes. Patients in the experimental group received 20? traction from below lumbar to bed,while patients in the control group received plane traction combined with longitudinal rotation. ④Lumbar function was determined by criterion of the lumbar disease made by Japanese Shaping Association to observe improved indexes and times of treatment. RESULTS:Totally 71 patients were involved in the result analysis. No significant difference was found in preoperative lumbar function between experimental group and control group(13.684?1.662,13.485?3.001,P