The strategies for constructing pharmaceutical subject repository union in China and the development trend of pharmaceutical subject repository union were studied form the aspects of its cooperation models, platform building, resources acquisition and organization, access policy formation;copyright, quality control, and fund source.The cooperation models were divided into centralized storage model, distribution acquisition model and hierarchical construction model.Pharmaceutical subject repository union platform was built according to the different cooperation models.

Objective:To evaluate the emergency diagnosis of severe myocardial contusion. Methods:Thirty patients with serious blunt chest trauma underwent sonourethrography,all of whom underwent conventional myocardial markers simultaneously.All the observation time phases were set as 1 h,2 h,4 h and 8 h after injuries. Results:Both the concentrations of cardiac troponin T (cTn-T)and cardiac troponin (IcTn-I)at 8h after injuries were significantly higher than those at 1h after injuries(P

Objective To study chronic bronchitis rats oxidant/antioxidant related indicators Ho flavored Dingchuan Decoction.Methods 50 SD rats were adopted improved smoked method to make chronic bronchitis model.From the 20th day of the modeling,blank control group and model control group gavage were treated with saline.The positive control group were treated with Cough Mixture.The experimental groupwere dealed with Ho flavored Dingchuan Decoction high,medium and low-dose.20 day later,the cotent of SOD,CAT,MDA in rat serum,lung tissue and balf were observed.Results Compare to blank control group,the cotent of SOD[(188.11 ± 9.37)U/ml、(276.11 ±30.28) U/ml,(8.80 ± 1.49)U/ml],CAT[(9.40 ±2.04)U/ml,(26.57±4.26)U/ml,(1.58±0.26) U/ml] in rat serum,lung tissue of the experimental group significantly reduced (P ＜0.01) ; MDA [(5.64 ± 0.52) μmol/L,(6.32 ± 2.10) μmol/L,(28.08 ± 2.43) μ mol/L) increased (P ＜0.05 or P ＜ 0.01).Ho flavored Dingchuan Decoction can effectively reverse these changes,and showed a significant dose-dependent.The Ho flavored Dingchuantang,in middle and high-dose serum SOD [(253.05 ± 7.81) U/ml,(256.92 ± 5.37)U/ml],CAT [(14.86 ± 2.49)U/ml,(18.22 ± 2.65)U/ml] higher than those of blank control group.And the Ho flavored Dingchuantang,in middle and high-dose lung tissue SOD [(470.91 ± 11.46)U/mgprot,(482.91 ± 14.32)U/mgprot),CAT [(46.87 ± 3.75)U/mgprot,(55.49 ±3.33)U/mgprot] higher than those of blank control group.Content close to the normal level.Conclusion The Ho flavored Dingchuan Decoction has significantly reduce the oxidative stress in chronic bronchitis.

AIM: To investigate the clinical effect of compound anisodine on patients with paralytic strabismus and the influence on malondialdehyde ( MDA ) and superoxide dismutase ( SOD) .
●METHODS: Seventy cases diagnosed with paralytic strabismus from Jun. 2008 to Dec. 2014 were selected as treatment group; another 70 cases with paralytic strabismus from Oct. 2000 to Jan. 2008 were chosen as control group. The control group was given with conventional therapy, and treatment group was additionally injected with compound anisodine subcutaneously on the basis of the control group. Treatment lasted for two courses about 28days. The effect of compound anisodine on patients with paralytic strabismus was evaluated and the serum level of MDA and SOD was also measured before and after treatment.
●RESULTS: ln treatment group, 54 cases were cured (77%), 14 cases improved (20%) and 2 cases had no response ( 3%) and total efficiency reached to 97%. ln control group, 32 cases were cured ( 46%) , 21 cases improved ( 30%) and 17 cases had no response ( 24%) and total efficiency was 76%. The cure rate and total efficiency of treatment group were significantly higher than that of control group (P<0. 05). Before treatment, the level of SOD and MDA was equal in two groups. After treatment, increased SOD level and decreased MDA level was observed in both groups. However, compound anisodine were significantly increased SOD level and reduced MDA level when compared with conventional therapy (P<0. 05).
●CONCLUSION: The compound anisodine shows the beneficial effect on patients with paralytic strabismus. Compound anisodine may exert the effect via increasing SOD and reducing MDA.

OBJECTIVE: To explore the materials and design of intraocular lens for traumatic cataract caused by sports injuries.METHODS: The databases of Medline and CNKI were retrieved by computer with key words of "sports injury, cataract, and intraocular lens". The languages were restricted as Chinese and English. By searching, 12 literatures concerning intraocular lens for traumatic cataract caused by sports injuries were selected, including 3 written in English and 9 in Chinese.RESULTS: One of the causes for cataract is sports injury. Every year, thousands of cases suffered eye damages in China due to sports. Here, first, a brief introduction of the causes of cataract was presented, and then the suitable intraocular lens implantation for athletes was prepared, finally, the materials and design of intraocular lens was discussed.CONCLUSION: The intraocular lens is developed fast both in basic theory and clinic, which exhibit good features in biocompatibility, visual function, regulatory ability, as well as photoprotection following transplantation. The widening of intraocular lens can provide more choices for physicians, and the life quality of patient was obviously improved.

Objective: To analyze the Effect of isokinetic training on knee injury patients’ recovery function. Methods: Choose knee injury patients between October 2012 to October 2013 as research object, randomly divided into control group received conventional training, and observe group received isokinetic training, each with 49 cases, detecting knee function after training, stretching flexor muscle strength, range of motion. Results: 1) After training ,both groups of patients inflammation Lysholm knee function scores were higher than before,and observation group patients Lysholm knee function score were significantly higher; 2)After training, both groups extensor flexor muscle strength were higher than before,and the observation group patients’ stretched flexor PT, AP, MRTW level were higher; 3)After eight weeks of training and 16 weeks, observation group patients’ knee extension, knee flexion angles were significantly higher.Conclusion:Isokinetic exercise training can improve knee joint function in patients, improve muscle strength while expanding its range of motion, improve the quality of life of patients.

Objective To analyze the prevalence of brucellosis with the initial symptom of fever in non-pasture area and to investigate the method of early diagnosis.Method Clinical features including symptoms,blood routine tests,blood cultures,rose-bengal plate agglutination tests,treatment and follow-up were analyzed in 18 patients with brucellosis admitted to the Department of Infectious Diseases,Tianjin First Centre Hospital from January 2010 to June 2013.Results Middle-aged men with history of smoking and drinking accounted for the majority of patients with initial symptom of fever.Twelve were not complicated with any underlying diseases.Twelve had no contact history.Four had history of farm animal contact,one had history of consuming raw meat products,and one had history of dog raising.All of them had fever,15 of which had hyperpyrexia and 13 had irregular fever.White cells in blood routine test were normal in 12 cases,decreased in 6 cases.And the proportion of rhabditiform nucleus neutrophil increased.Six showed neutrophil left shift.Blood platelet decreased in 3 cases.Thirteen cases showed increased alanine aminotransferase and aspartate aminotransferase.C-reactive protein was elevated in 14 cases.Twelve cases had positive culture of Brucella melitensis in blood or bone marrow culture.Eighteen cases had positive results of rose-bengal plate agglutination test.The proportions of neutrophil and lymphocyte were normal.The treatment included moxifloxacin,levofloxacin,isepamicin,etimicin,amikacin,rifampicin,minocycline.The regimens were usually two or three drugs in combination.The duration of follow-up ranged from 4 to 11 months with good prognosis.Conclusions Patients from nonpasture area with initial symptom of fever could be brucellosis.Clinician should be alert to the disease and inquiry contact history in detail.Brucella serology test should be performed.Early diagnose and prompt treatment could avoid serious complications.

Objective To investigate the effect of early embryonic mouse pancreatic tissue transplantation in the treatment of mouse experimental diabetes.Methods C57BL/6 mouse models of streptozocin-induced diabetes mellitus were established and then randomly divided into two groups:transplantation group,in which,five to seven pieces of pancreatic tissue of mice at embryonic 16.5 days were transplanted into mouse renal capsule,and sham-operated (SO) control group,in which,0.05 ml RPMI1640 culture medium was injected into mouse renal capsule.When blood glucose level of the transplantation group mouse was ≤ 11.2 mmol/L,the endocrine function of embryonic pancreatic tissue transplanted was detected by IPGTT and IPITT methods and then the transplanted graft was removed for observing the blood glucose surge.In vitro,levels of insulin secretion were measured in serum,E16.5 pancreatic tissue and graft (3.3 mmol/L and 16.7 mmol/L) glucose stimulation by ELISA.Histology and immunohistochemistry were observed before or after pancreatic tissue transplantation to detect insulin and glycagon production.Results (1) The glucose level decreased significantly in the TX group after 4-6 weeks of transplantation (13.4 ± 6.5 vs 28.9 ± 2.5,P ＜ 0.05),and the body weight gaining (P ＜ 0.05).Compared to SO group,glucose level decreased significantly (P ＜ 0.001).The graft can modulate insulin secretion; (2) Insulin can be detected in E16.5 embryonic mouse.Insulin and glycagon were produced more in TX group than pretransplantated pancreatic tissue.Conclusions E16.5 embryonic mouse pancreatic tissue transplantation effectively controls serum glucose level and restore the nondiabetic pattern of weight gaining in diabetic mouse.

Objective To observe the effect of penehyclidine hydrochloride (PHCD) combined with mechanical ventilation on inflammatory response in rats with acute respiratory distress syndrome(ARDS)induced by hydrochloric acid(HCl)inhalation. Methods 32 adult healthy male Sprague-Dawley(SD)rats were randomly divided into four groups:sham operation group,ARDS model group,PHCD group(intraperitoneal injection of 0.5 mg/kg PHCD at 30 minutes before HCl instillation)and mechanical ventilation group〔mechanical ventilation was given after PHCD treatment,ventilator settings were as follows:tidal volume(VT)=4 mL/kg,respiratory rate(RR)= 70 beats/min,the time ratio of inspiration to expiration(I:E)=1:2,fraction of inspired oxygen concentration(FiO2)=0.21〕,8 rats in each group. The rat ARDS model was created by HCl(0.1 mol/L,1.2 mL/kg) intratracheal instillation. After mechanical ventilation for 4 hours,the experiment was stopped. Arterial partial pressure of oxygen(PaO2)was measured,and the oxygenation index as well as lung wet/dry weight ratio(W/D)were calculated respectively. The pathological changes in lung tissues were observed under light microscope,and the pathological score was evaluated. The levels of myeloperoxidase(MPO),interlukin-8(IL-8)and nuclear factor-κB (NF-κB)in homogenate of lung tissues were all measured respectively by enzyme linked immunosorbent assay (ELISA). Results In rats of ARDS model group, there were significant infiltration of inflammatory cells, collapse of extensive pulmonary alveoli and hyaline membrane formation in lung tissues,but the edema in pulmonary mesenchymal tissue and congestion of micro vessels were not obvious. Compared with sham operation group,the lung tissue pathological score(9.88±2.03 vs. 1.38±0.92),lung W/D ratio(7.67±0.96 vs. 4.08±0.65)were significantly increased(both P<0.01),while oxygenation index〔mmHg(1 mmHg=0.133 kPa):213±29 vs. 428±28〕was markedly decreased in model group(P<0.01);and the concentrations of MPO(μg/L:34.18±3.99 vs. 20.92±1.40), IL-8(ng/L:864±53 vs. 583±91)and NF-κB(ng/L:1 229±133 vs. 803±130)in homogenate of lung tissues were significantly higher in model group(all P<0.01). The pathological changes in lung tissues including inflammatory cell infiltration,collapse of alveoli and pulmonary edema,etc. were obviously improved when treated by PHCD or mechanical ventilation and the lung pathological score and W/D ratio were significantly decreased compared with those of model group,oxygenation index in these two groups were both significantly increased,meanwhile, the concentrations of MPO,IL-8 and NF-κB were sharply decreased when compared with those of model group (all P<0.05). The degree of improvement in all above indexes were greater in mechanical ventilation group than those in PHCD group(lung injury score:5.63±1.85 vs. 7.63±1.67,lung W/D ratio:5.35±1.05 vs. 6.46±1.17, oxygenation index:325±57 vs. 272±44,MPO:25.80±3.36 vs. 29.88±3.93,IL-8:736±53 vs. 802±53, NF-κB:984±75 vs. 1 109±118,all P<0.05),however they did not reach the levels of the sham operation group (P<0.05 or P<0.01). Conclusion PHCD can inhibit the inflammatory response in ARDS rats induced by HCl inhalation,thus it may protect the lung tissue from injury induced by HCl,and the protective role of PHCD plus menchanical ventilation is superior to that of PHCD only.

Circulating tumor cells (CTCs) play pivotal roles for monitoring the tumor metastasis and prognosis.The nanotechnology provides a favourable platform for CTCs detection,and enables CTCs to be more promising for practical application.Meanwhile,the nanoscale device by virtue of nanotechnology has broad application prospects in eliminating CTCs and offers a new direction in the field of anti-cancer.