Objective: To investigate the inhibitory effect of Green Tea Polyphenols(GTP) on H2O2-induced lens epithelial cell apoptosis and relevant mechanism.Methods: Separated and cultivated lens epithelial cell,disposed that with GTP,then detected the inhibitory effect of GTP by electron transmission microscopy,flow cytometry and DNA fragmentation assay.The bcl-2,Bax and caspase-3 protein expression were measured with Western-blot in various groups.Results: The apoptosis cells and G1 phase cells increased in the group treated with sodium chloride solution,and the DNA "ladder" was found.In the group treated with GTP,the apoptosis cells and G1 phase cells decreased,and no DNA "ladder".The apoptosis protein bcl-2 increased,however,the Bax and caspase-3 protein decreased.Conclusion: The GTP can protect lens epithelial cells from the oxidative injury and restrain the apoptosis rate of lens epithelial cells and the mechanism was nearly correlated with bcl-2,Bax and caspase-3 protein.

Anastomotic leak after aortic dissection surgery is a common and critical complication.Interventional therapy is less invasive and effective.This case reported that anastomotic leak was found after surgery for type A aortic dissection.The risk of reoperation was high.The minimally invasive method was used to release the ocduder to the anastomotic leak.The effect was accurate.

QT dispersion(QTd,equals maximal minus minimal QT interval on a standard ECG) has been shown to reflect inhomogeneity of myocardial repolarization and cardial electrical instability, hence,been proposed to be associated with ventricular arrhythmias.But the intrinsical mechanism of QTd is incompletely understood. Contributing to QTd are differences of action potential duration(APD) in the three-dimensional structure of ventricular myocardium, which is identified to own three cell subtypes:endocardial, midmyocardial(M cells) and epicardial cells.And findings suggest that regional differences in the duration of the M cell action potential may lead transmural and spatial dispersion of repolarization. Autonomic nerves by combining receptors on the myocardial cells,especially M cells affect significantly the APD.Concordance between heterogeneity of ventricular myocardium and heterogeneity of autonomic innervation in heart provide support for the role of autonomic nerve in the generation of the QT dispersion.

Apoptosis ; Fatty Liver, Alcoholic ; Humans ; Lipid Metabolism

Objective To estimate the effect of low molecular weight heparin (LMWH) combined with conventional treatment on patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods Literature on randomized control trials( RCTs) with AECOPD treated by LMWH combined with conventional methods was collected in PubMed.The studies were selected according to inclusive and exclusive criteria.The data were extracted,the quality was assessed, and meta-analysis was conducted with RevMan 5.2 software.Results There were twelve RCTs including 879 patients with AECOPD in the current study.The results of meta-analysis showed that:①LMWH could obviously improve PaO2 and PaCO2 of patients with AECOPD as compared with the control group.②There was no statistically significant difference between the LMWH-treated group and control group in the improvement of FEV1 or hematocrit.Conclusion LMWH can significantly improve arterial blood gas analysis in AECOPD, but does not improve FEV1 or hematocrit.

Background Cell immunologic therapy for retinoblastoma(RB)is becoming a hot research topic.Cancer-testis antigen is a human immunogenic protein and is used to treat some tumors.However,its effect on RB has not been investigated.Objective The present study was to discuss the antigen specific anti-tumor effect of cytotoxic T lymphocytes(CTL)induced by the cancer-testis antigen,NY-ESO-1-sensitized dendritic cells(DCs),on human RB.Methods PCR was performed to amplify target gene fragments from the NY-ESO-1 plasmids,and then the target gene fragments were digested with the restriction enzymes SalI and EcoRI.Harvested fragments were inserted into the pDC316 plasmid to construct the recombinant plasmid pDC316/NY-ESO-1.The expression of NY-ESO-1 protein in human RB cells strain,HXO-RB44,was detected by immunofluorescence and Western blot.Monocytes were isolated from 60 ml of peripheral blood from a healthy donor using Ficoll density-gradient centrifugation with a cell density of 1 × 107/ml.DCs isolated from blood were stimulated with recombinant human granulocyte-macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin-4(rhIL-4).The recombinant plasmid pDC316/NY-ESO-1 was transfected into DCs and the DCs were co-cultured with T lymphocytes.The resultant CTL were used as effector cells.The growth of the CTL was detected by MTT assay.The CTL were then added into the growth medium used for culturing HXO-RB44 cells and the vitality of the HXO-RB44 cells was assayed by MTT assay.Results The sequence of the cloned DNA fragment of the recombinant plasmid pDC316/NY-ESO-1 was conforms with the sequence of the NY-ESO-1 gene.The expression of the NY-ESO-1 protein in HXO-RB44 cells was tested by immunofluorescence and Western blot.DCs were successfully induced with rhIL-4 and rhGM-CSF from PBMC.The recombinant expression plasmid pDC316/NY-ESO-1 was successfully transferred into DCs.These DCs had high expression of surface molecules such as HLA-DR(42.1％),CD80(54.2％),CD83(39.7％)and CD86 (94.8％).The CTL that was induced by DCs-sensitized with NY-ESO-1 specifically killed HXO-RB44 cells.CTL induced by the sensitized DCs had a stronger cytotoxic effect against HXO-RB44 cells compared with un-sensitized DCs and CTL un-induced with DCs,as shown by MTT asssay(P＜0.05).The anti-tumor activity was highest when the ratio of effector to target was 75∶1(P＜0.05).Conclusions DCs transfected by the recombinant plasmid pDC316/NY-ESO-1 can induce the proliferation of allogenic CTLs,which showed a specific anti-tumor effect against HXO-RB44 cells.These results present a new type of immunotherapy for the treatment of RB.

It is important to meet the need of modern high-technology war during the new period in which our army change the modes of generating combat capabilities .How to more scientifically and effectively improve the soldiers′physical quality and reserve ability will play a key role in soldiers′operations training .Therefore,the metabonomic analysis will pro-vide a new idea for the military physical training monitoring .The application of metabonomics to military fitness training monitoring is described in this paper .

Objective To explore the replantation method of upper limb's biplane mutilation in conjunction with soft tissue defect.Methods From January 2003 to January 2013,29 patients with upper limb's biplane mutilation in conjunction with soft tissue defect were treated.There were 21 males and 8 females with the average age of 28.6 years.The youngest one was 1 year old and the oldest one was 70 years old.All the cases underwent different replantation order and transplantation by using vascular and nerve and flap according to the lesion they suffered,including the defected plane and soft tissue.Results All replantation limbs were survived besides 2 cases with infection and 3 cases of delaying healed due to the part of tissue necrosis.Among all 29 cases,9 cases got excellent recovery,14 cases were in good condition,4 cases were poor and 2 cases were bad.The totally excellent and good rate of upper limb function was 79.3％ according to the limb reattachment function evaluation criteria of Hand Surgery of the Chinese Medical Association.Conclusion It can obtain higher survival rate due to our different replantations and method of tissue replantation to cure double plane mutilation in conjunction with soft tissue defect of upper limb.

Objective:The purpose of this study is to investigate the feasibility of labeling skin of inner mongolia cashmere goats with BrdU,and to optimize its immunohistochemical conditions.Methods:We injected different dose of BrdU into the neck vein of cashmere goats,then we took samples from the back skin of the goats,fixed in 4% paraformaldehyde.Next the samples were embedded with paraffin and sectioned.We examined the samples with immunohistochemical technology to test the effect of the influencing factors to labeling results as the BrdU injection,such as dosage,the concentration of second antibody and streptavidin-peroxidase,the way of antigen pretreatment,and DNA denature temperature.Results:We observed red BrdU positive cells in labeled goats whereas not in the control.Meanwhile,in the samples of the unlabeled goats or those incubated with PBS,no nuclear staining is observed.Conclusion:BrdU can be used for labeling of inner mongolia cashmere goats in vivo,which have no obvious dosage effect.The best condition of labeling is that puttig the section in 2 mol/L HCl 30 min at 37℃,in trypsin 30 min at 37℃.The most appropriate concentration of biotin-conjugated second antibody and streptavidin-peroxidase is 1∶100,under which the results of immunohistochemical are the best.

Effects of opiate receptor blocker-naloxone ( 5mg/kg) on somatosensory evoked potentials ( SEP ) in 6 cats was studied. Other 6 cats were treated with normal saline as control. Results showed that the amplitudes of SEP were significantly elevated after 0.5h of aaloxone infusion. The changes of SEP-CCTp or CCTmn were no significant. It was suggested that large dosage of naloxone can favorably improve the electrical activites of neuron in the centre nerve system.