Pathogen incubation theory includes "no manifestation after infection" and "manifestation after incubation". Clinical data showed that the incidence and recurrence of tic disorders in children had a strong relevance to six exogenous factors. The pathogenesis is similar to the pathogenic mechanism based on incubation of pathogen theory, so we proposed a theory of "tic disorder induced by incubation of pathogen". Pathogenic wind can be classified into exterior wind and endogenous wind. Pathogenic wind is more apt to move, rise and migrate. The characteristics of pathogenic wind, especially easy mobility, determine the symptoms and signs of tic disorder, for pathogenic wind can be characterized by vibration and involuntary movement such as convulsion and tremor. If exogenous pathogenic wind moves into half-exterior and half-interior phase from the exterior, both the exterior and interior syndromes should be treated at the same time. We should regulate the function of the liver and the lung, expel pathogenic wind by dispersing the lung, and calm endogenous wind by removing obstruction in the collaterals and soothing the liver.

Objective To establish the method for detecting human Bocavirus (HBoV) by a real-time fluorescent quantitative PCR (FQ-PCR) and to assay HBoV from nasopharyngeal samples of children with acute respiratory tract infections.Methods The specific primers and Taqman probes of targeted HBoV NP1 gene were designed.The aimed fragment of NP1 gene was amplified with PCR and ligated into a pCEM-T Easy vector to set up the standards.The method for detection of NP1 gene using FQ-PCR was established.The sensitivity and specificity of the method had been tested.Then 195 clinical specimens were subsequently tested.Results The specificity of this method was excellent.The linear amplification of the assay ranges from 10 copies/μl to 10~8 copies/μl.Twelve specimens were tested positive for HBoV in 195 clinical specimens (6.2%).Conclusions The method for detection of HBoV NP1 geneby real-time fluorescent quantitative PCR was established successfully.It can offer fast and reliable diagnosis for HBoV infection.

Chemokines are a group of small molecules that cause the directed migration of cells. In lung cancer, chemokines involve in the regulation of tumor cell growth, angiogenesis, anti-tumor immunity and remote metastasis, which provides a novel therapeutic target for lung cancer.

Objective To investigate the effect of scene-style education mode in endoscopic ultrasonography examination.Methods 170 patients undergoing endoscopic ultrasonography from May 2009to December 2011 in our hospital were chosen as the research object.They were randomly divided into the control group(conventional education mode group)and the observation group (scene-style education mode) with 85 cases in each group.The repeated rate of insertion,rate of complication,compliance degree,the POMS scale score,blood pressure and heart rate for the two groups of patients were compared.Results Compared with the control group,the repeated rate of insertion,rate of complication were lower,blood pressure and heart rate were lower,and compliance degree and POMS mood scale score were better in the observation group,there were significant differences.Conclusions The scene-style education mode can improve negative emotions of patients undergoing endoscopic ultrasonography significantly,it also plays a positive role in increasing their compliance degree and easing stress response.

Objective To investigate the expression of high mobility group protein box1 (HMGB1)in ovarian endometriosis tissue and to provide new idea for early diagnosis,treatment and prognosis of endometriosis.Methods The levels of HMGB1 in ectopic endometrium and eutopic endometrium of 69 patients with ovarian endometriosis and endometrium of 69 cases with non-endometrioses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting.Results HMGB1 was expressed in all the three groups (ectopic endometrium eutopic endometrium endometrium with non-endometrioses).The relative expression level of HMGB1 mRNA was 0.54383 ± 0.0565,0.3016 ± 0.0614,and 0.1536 ±0.0444,respectively (F =377.923,P ＜ 0.0001).The relative of HMGB1 protein was 0.83990 ±0.12869,0.40100 ±0.16425,and 0.19986 ± 0.08162,respectively (F =185.625,P =0.0000).The expression level of HMGB1 was related to clinical stages (t =10.28,P ＜0.01) ; however,it was not obviously related to age,the diameter of lump,and course of disease (P ＞ 0.05).Conclusions Over expression of HMGB1 in ovary endometriosis indicates that HMGB1 plays a significant role in occurrence and development of ovary endometriosis.The expression of HMGB1 in endometriosis eutopic endometrium was much higher than that of non-endometrioses endometrium,which supports the eutopic endometrium determinism.The overexpression of HMGB1 was consistent with clinical stage,which proves that HMGB1 maybe be tightly associated with the severity of the disease and to likely become biomarker.

Objective To evaluate the efficacy and side-effects of the levonorgestrel-releasing intrauterine device (Mirena) in the treatment of dysfunctional uterine bleeding .Methods 45 cases of dysfunctional uterine bleeding diagnosed by pathology were installed with Mirena.The endometrial thickness by ultrasound hemoglobulin increase and pathologic changes of endometria were recorded before and 24 months after the installation.ResultsThe endometrial thickness before and after the insertion of Mirena were(15.6 ±4.2) mm and (3.2 ± 1.3)mmrespectively (P ＜ 0.01) .Six months after the insertion of Mirena, patients' hemoglobulin was significantly increased from (97.6 ± 8.2)g/L to (123.7 ± 8.1)g/L (U = 2.26, P ＜ 0.05) .After the installation of Mirena, patient experienced metrorrhagia.Conclusions Mirena is a device providing effective and continual therapy for dysfunctional uterine bleeding .It can not only significantly reduce the menstrual blood loss but also reverse the complex hyperplasia effectively.

Objective To explore the effect of continuous hyperglycemia on learning and memory capacity in rats, and to observe the changes of expressions of ubiquitin and apotosis-related proteins, so as to provide a relative basis for diabetic encephalopathy. Methods The male SD rats were randomly divided into normal control group and diabetes mellitus (DM) group. The DM rats was made by intraperitoneal injection of streptozocin (STZ). The capacities of learning and memory were tested by Y-maze. The structure changes in frontal lobe cortex and hippocampus were observed by Nissl's staining and immunohistochemistry. The apoptosis cell count was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The expressions of Bcl-2 and P53 were assessed by Western-blot analysis. The expression of ubiquitin mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results In DM rats, the learning and memory capacities were worse than in control rats (P＜0. 05), and the apoptosis cell counts in frontal lobe cortex and hippocampus were higher than in control rats, the expression of Bcl-2 gene was reduced while the expressions of ubiquitin and P53 gene were increased (P＜0. 05). Conclusions In the frontal lobe cortex and hippocampus, the expression of Bcl-2 gene is reduced while the apoptosis cell numbers and ubiquitin P53 gene expression are increased, which may contribute to neurodegeneration in DM rats.

Human resources management as the core factors affects the development of hospital library. The management should con-sider people -oriented theory, integrate harmonious management and concept innovation, and pay more attention to development and uti-lization of human resources, so as to ensure staffs would play an important role in hospital library modernization construction and sustain-able development.

Objective To research into the relationship between heart rate variability (HRV) and QT dispersion(QTd) in patients with type 2 diabetes mellitus(T2DM) complicated with coronary heart disease(CHD).Methods In the research 30 patients with type 2 diabetes mellitus complicated with CHD were classified as group A,33 patients with T2DM as group B and 21 normal healthy persons as controlgroup,group C.24-hour time domain and 24-hour frequency domain parameters of HRV were recorded by holter ECG and analysed through computer.A standard 12-lead synchronous electrocardiogram was used to measure QT interval and QTd.Results (1)The research showed that all parameters of HRV in group A were less than those in group B and group C (P

Ma Peizhi was the representative of MenHe Medicine;he learned from Ma Shengsan and Fei Boxiong,Wang Jiufeng.He was thoroughly mastered and continually blazed new trails;Ma Peizhi’s Academic Thoughts of Surgery of Traditional Chinese Medicine is still the guidance on the modern clinical experience.