High resolution melting ( HRM) analysis is a real-time PCR based technique which can detect DNA sequence polymorphism .Immediately after routine PCR with saturated fluorescent dye , melting transition of the amplicon was monitored and fluorescence was collected .According to the different melting curve, single base differences can be distinguished .HRM is a low -cost, single -step, closed -tube, accurate and rapid method , which has aroused general concern .Rapid and sensitive detection of the clinical pathogens is critical to diagnosis of infectious diseases .However , clinical research on application of HRM in infectious disease pathogen identification and drug resistance , especially melting curve analysis based bedside technology integration will provide strong support for the diagnosis and treatment of infectious diseases.

Rapid on- site evaluation (ROSE) had gradually become an essential tool in interventional section, especially in pulmonary diseases. People were no longer limited to routine endoscopic operation. ROSE combined with fine-needle aspiration (FNA) enhanced the diagnostic efficiency and diagnostic accuracy of routine bronchoscopes, endobronchial ultrasound- guided transbronchial needle aspiration (EBUS-TBNA) and percutaneous lung biopsy. ROSE can read out the specimen on site , give preliminary diagnosis, and provide help for next operation. The ROSE included cytological ROSE (C-ROSE) and microbiological ROSE (M-ROSE). Now in clinic, C-ROSE was used more broadly. C-ROSE expanded the check range of routine endoscopic operation, and it had been proven to be safe and useful tools.

With reference to medical personnel's infection during the prevalence of SARS, the paper offers some general information on the risks of occupational exposure of medicine. The commonly seen risks fall into four major categories, including biological risks, such as infection of hepatitis B, hepatitis C, HTV, etc.; chemical risks, such as those from antineoplastic drugs, cleaning agents, disinfectants, narcotics, dusts; physical risks, such as radiation, injury by sharp instruments, injury from heavy burden, forced body positions, etc.; and psychological risks. The paper also puts forward some proposals for safety protection: intensifying training in occupational safety of medicine, reinforcing the protection of high-risk groups of people and high-risk body parts, augmenting the supply of protective materials and the improvement of protective facilities, and setting up and perfecting long-acting protection and supply mechanisms.

Currently,since a single treatment standard of benign tumors of liver is not established,many serious out-comes due to the excessive treatment of benign tumors of liver still happen sometimes.We should pay attention to composing the guideline on the treatment for the different types of benign tumors of liver on the basis of the multidisciplinary cooperation and the randomized controlled trials,and achieving the goals of an unified standard and the standardized therapy,meanwhile,avoiding the pain and burden of excessive treatment on the patients.

BACKGROUND:Induced pluripotent stem cels have been a hotspot in regenerative medicine research since it was discovered. The clinical application of induced pluripotent stem cels is excessively focused on, but the safety issue is almost ignored. OBJECTIVE:By summarizing the application of induced pluripotent stem cels in animal experiments to analyze the safety problems of induced pluripotent stem cels and their possible reasons in order to lay a foundation for further study and clinical application of induced pluripotent stem cels. METHODS: PubMed database was retrieved by the first author for articles related to the safety of induced pluripotent stem cels published from 2006 to 2014 using the keywords of “induced pluripotent stem cels, safety, immune, immunogenicity, tumorigenicity, cancer, epigenomic, transplantation, generation, reprogramming,genomic, mutation” in English. Related ful texts were got from Cel Press and Nature Databases. Finaly, 28 articles were chosen in result analysis. RESULTS AND CONCLUSION: Safety problems of induced pluripotent stem cels are attracting more and more attentions. Immunogenicity, potential tumorigenicity and epigenetic variation are major risks for the clinical applications of induced pluripotent stem cels. Safety issues of induced pluripotent stem cels mainly come from the reprogramming process. The “integrating genetic manipulation” may lead to a greater risk of tumorigenicity than non-integrating operations. Epigenetic variations emerge in the reprogramming, which are mostly relative to “epigenetic memory” of reprogrammed cels.

Background and purpose: MicroRNA(miRNA) is a class of small non-coding RNA playing an important regulatory role in many diseases. In this study, we explore the levels of miR-192 and zinc ifnger E-box binding homeobox 2 (ZEB2) in CRC, the clinical signiifcance of miR-192 and the correlation between the expression of miR-192 and ZEB2 protein. Methods: The expression levels of miR-192 and ZEB2 mRNA in 30 colorectal carcinoma samples, 30 corresponding cancer-adjacent tissue samples (from the edge of tumor≥5 cm), 25 colorectal adenoma samples, and 15 normal tissue samples were quantiifed using quantitative real-time polymerase chain reaction (qRT-PCR). ZEB2 protein expression was determined using Western blot. The relationship between the miR-192 and clinicopathological factors, miR-192 and ZEB2 protein expression was analyzed. Results:Signiifcant upregulation of miR-192 expression and reduction of ZEB2 mRNA and protein expression were identiifed in CRC tissues, compared to cancer-adjacent tissues, colorectal adenoma samples, and normal tissues (P<0.05). Low miR-192 levels were signiifcantly associated with lymph node (P=0.021) and distant metastasis (P=0.023). An inverse relationship between miR-192 and ZEB2 protein expression was identified in CRC group (r=-0.365, P<0.05). Conclusion: MiR-192 downexpression was correlated with CRC metastasis. MiR-192 may play a role in the development and progression of CRC through ZEB2.

With the rapid development of modern technology, some new ideas and equipments have been successfully used to resolve one of worldwide problem- rehabilitation of cerebral palsy. Adeli suit treat-ment,a new technological method,which selects spacesuit as the main equipment of intensive training,now has been tentatively used in the United States and Europe. This article focuses on the principles,methods and efficacy of Adeli suit treatment in order to provide a new rehabilitation method for cerebral palsy.

Although the cesarean section has become an effective means to solve dystocia,high - risk pregnan-cy and other critical obstetrical disease,but the morbidity rate and hospitalization rate of newborn did not decreased obviously because of the continuous increasing of cesarean section rate. Contrary,in recent years,many domestic and foreign researches have found that,compared with normal vaginal delivery babies,cesarean section newborn infants have higher related disease incidence rate after birth,especially respiratory distress sydrome,transient tachypnea of newborn infants,persistent pulmonary hypertension and infection,and more attention should be paid to the fact that cesarean section children are more prone to allergic and immune system diseases as well as neuropsychiatric related diseases. Therefore,this article aims at integrating the researches and providing an overview on recent and long - term effection on newborn infants.

Objective To investigate the efficacy of sertraline hydrochloride combined with mesalazine in the treatment of patients with ulcerative colitis complicated with depression .Methods From March 2014 to March 2015 ,eighty patients with mild active ulcerative colitis ,self‐rating depression scale (SDS) score between > 53 and 70 points ,were enrolled .Eighty patients were divided into sertraline hydrochloride combined with mesalazine group and mesalazine group (control group) ,40 patients in each group .SDS questionnaire and gastrointestinal endoscopy were applied at the beginning of the study and 12 weeks after treatment to evaluate the efficacy .Chi square test or t test was performed for statistical analysis .Results After 12 weeks treatment ,the efficacy of mesalazine combined with sertraline group and mesalazine group was 75% (30/40) and 65% (26/40) , respectively ,and the difference was not statistically significant ( P > 0 .05 ) .Before the treatment ,ulcerative colitis activity index (UCAI) of mesalazine combined with sertraline group and mesalazine group was 13 .13 ± 4 .41 and 12 .41 ± 3 .96 , respectively ;12 weeks after treatment ,the UCAI of the two groups were 7 .29 ± 5 .78 and 8 .60 ± 4 .56 , respectively .The UCAI of both the two groups were lower than those before treatment , and the differences were statistically significant (t= 6 .106 7 and 5 .189 3 ,both P < 0 .01) .The difference was not statistically significant in UCAI between the two groups after treatment (P > 0 .05) .Before treatment ,SDS score of mesalazine combined with sertraline group and mesalazine group was 55 .98 ± 9 .61 and 56 .27 ± 8 .45 , respectively ;12 weeks after treatment ,SDS scores of the two groups were 49 .36 ± 4 .51 and 52 .81 ± 6 .11 ,respectively .Compared with those before treatment , after 12 weeks treatment , SDS scores of mesalazine combined with sertraline group and mesalazine group both decreased (t= 3 .944 0 ,P= 0 .000 2 ;t=2 .098 6 ,P= 0 .039 1) .After treatment ,SDS score of mesalazine combined with sertraline group was lower than that of mesalazine group (t = 2 .873 2 , P = 0 .005 2) .Conclusion Routine ulcerative colitis treatment combined with sertraline hydrochloride in ulcerative colitis can significantly improve depression of ulcerative colitis patients and improve the quality of patients′ life .

To compare the clinical efficacy of multi-needle shallow puncture with conventional acupuncture for treating simple obesity. Methods: According to the principle of evidencebased medicine, 40 simple obesity patients were randomly allocated to a multi-needle shallow puncture group of 20 cases and a conventional acupuncture group of 20 cases.Treatment was given once daily, 20 times in total. Body weight, standard body weight, body mass index, fat percentage, chest circumference, waist circumference and hip circumference were recorded before and after treatment. The obtained data was analyzed. Results: Body weight and waist circumference significantly changed in both groups after treatment. The curative effect was significantly better in the multi-needle shallow puncture group than in the conventional acupuncture group (P＜0.05). Conclusion: Multi-needle shallow puncture has clinically a good effect on simple obesity. Its effect is better than that of conventional acupuncture.