Objective To investigate influence and significance of preoperative transcatheter arterial chemoembolization (TACE) on disease-free survival rates and overall suvival rates in patients with respectable large hepatocellular carcinoma(HCC). Methods One hundred and eight patients with respectable large primary liver cancers were prospectively randomized into two groups: preoperative TACE group(n=52) and one-stage operation group(n=56). We compared between two groups according to daughter nodules and tumor thrombus. Results There were no significant differences in the incidence of disease-free survival rates and overall suvival rates in the two groups. Conclusion Preoperative TACE for resectable large HCC should be used on bases of strict selection because it does not decrease the recrudescence rates and results in delayed surgery in some cases.

Recently,basic and clinical researches have shown that blood glucose variability is an independent risk factor for diabetic complications.Thus,ideal control of blood glucose is not only to reach the HbA1Ctarget,but also to minimize blood glucose variability.Different glycemic parameters could be selectedaccording to different purpose of evaluation,in which the mean amplitude of glucose excursions(MAGE) and the standard deviation of blood glucose (SDBG) could be used as accurate and simple assessment parameters respectively.

Currently,since a single treatment standard of benign tumors of liver is not established,many serious out-comes due to the excessive treatment of benign tumors of liver still happen sometimes.We should pay attention to composing the guideline on the treatment for the different types of benign tumors of liver on the basis of the multidisciplinary cooperation and the randomized controlled trials,and achieving the goals of an unified standard and the standardized therapy,meanwhile,avoiding the pain and burden of excessive treatment on the patients.

The treatment principle for primary liver cancer referring as hepatocellular carcinoma (HCC) in this article is comprehensive treatment based on radical hepatectomy. Focusing on improving the resection rate and reducing the recurrence rate after operation, the conversion therapy, preoperative neoadjuvant therapy and postoperative adjuvant therapy have emerged to become potentially curative strategies and research hotspots. HCC is highly invasive and often complicated with cirrhosis, so comprehensive treatment is essential for better prognosis. However, due to the controversy of surgical indications and heterogeneity among individuals, the concepts and treatment methods of above three therapies, especially between the conversion therapy and the preoperative neoadjuvant therapy, remain overlap and puzzle sometimes. Moreover, because of the different therapy goals, the above three therapies are different in the selection of treatment methods, duration of therapy and evaluation criteria. Based on clinical practice, the authors investigate the progress and hot spots of the conversion therapy, preoperative neoadjuvant therapy and post-operative adjuvant therapy of HCC, expecting more clinical evidence-based medicine to standardize the development of comprehensive treatment, so as to become a breakthrough to improve the diagnosis and treatment of HCC.

Objective To discuss the main features and experiences of surgical treatment of congenital spinal bifida and tethered cord syndrome. Methods Spinal bifida and tethered cord syndrome are congenital malformations. A study was conducted to analyse the morphology and the microsurgical operation of the diseases. During the Sept, 1993 to Feb, 2003, 528 patients were admitted to the hospital. The age ranged from 5 months to 45 years old (mean age 9 years). 239 cases(45.3%) were diagnosed as myelomeningocele, 123 cases(23.3%) were meningocele, 107 (20.3%) were spinal bifida with lipoma, and 59 cases of spinal bifida occulta (1.2%). Surgical removal of the arachnoid cyst and lipoma relieved compression of the spinal dural sac and the tethered spinal cord. Results Improvement was achieved in 322 cases (61%), no improvement in 194 cases (36.7%), and exacerbation of symptoms in 12 cases (2.3%). Conclusion Careful planning for the surgical treatment of spinal bifida is imperative. It is also important to release the compression tissues, in the spinal canal and to loosen the tethering.

Objective :To study the effect and mechanism of retinoic acid(RA) on thymocytes development. Methods:Thymus tissue culture system was used to investigate the roles of RA in maturation development of thymocytes. Thymus tissues were cultured with or without retinoic acid and/or retinoic acid receptor antagonist. The cultures were harvested after 24, 48 and 72 hours, respectively. Thymocytes were stained with fluorescence labeled anti-CD3, anti-CD4, anti-CD8 antibodies and analyzed by flow cytometry. The real time quantitative RT-PCR assay was used to detect the retinoic acid receptor (RAR) mRNA expression in thymocytes. Results:The thymus tissue culture system was able to support the maturation of thymocytes. Addition of RA to the culture system promoted thymocytes differentiation from immature CD4+CD8+ cells to mature CD4+ cells, but inhibited the transformation from immature CD4+CD8+ cells to mature CD8+ cells. The effect was marked after 24h culture, and was antagonized by Ro41-5253. RA enhanced the expression of RAR mRNA in thymocytes. A positive correlation was found between the expression of RAR mRNA and the percentage of CD4+CD8+ cells and a negative correlation between the expression of RAR mRNA and the percentage of CD8+ cells. Conclusion:RA likely play an important role in thymocytes development, perhaps by affecting the relative expression of RAR gene.

Objective To study the effects of light damages of epidermal permeability barrier on cosmetics function evalu-ation.Methods 10volunteers were selected during the1st stage i.e.the3-month experiment of cosmetics function carried out using the traditional method.The skin moisture,transepidermal water loss and skin elasticity were measured,and the required sample size was estimated also.The2nd stage of the experiment of cosmetics function was carried out based on the data of the1st stage.The skin surface was cleaned with the detergent containing sodium lauryl sulfate with mass fraction of5%to induce light damages in skin surface before testing with cosmetics every day,those indexes mentioned above were measured also.Re -sults The1st stage of the experiment using the traditional evaluation method showed that the skin moisture,transepidermal wa-ter loss and skin elasticity revealed significant differences in the3rd month after testing with cosmetics compared with those be-fore testing with out cosmetics(P

MicroRNA (miRNA) is a class of endogenous,single-stranded non-coding small RNA that contains 21 to 23 nucleotides and is widely distributed in eucaryon,miRNA plays an important regulatory role in cell proliferation,apoptosis,growth and development through the regulation of gene translation after transcription and expression,miRNA has a close relationship with pathogenesis and development of hepatocellular carcinoma.And in this process,part of miRNA acts as oncogenes or tumor suppressor genes.A number of miRNA,such as miR-30d,miR-221,miR-222 and miR-101,had been found to express abnormally in hepatoeellular carcinoma.Here we summarize the related progress in research of miRNA and hepatocellular carcinoma.

Hepatic vein hemorrhage and air embolism are easily caused during the resection of the tumor involving the second hepatic hilum.Hepatic vein occlusion has been proven to decrease this risk,while classic selective hepatic vein occlusion with tourniquet is technique demanding.We modified the classic method by using Satinsky clamp in hepatic vein dissection and occlusion.Based on the clinical data of 220 patients who received hepatic vein occlusion with tourniquet and 330 patients with Satinsky clamp,we proved that hepatic vein occlusion with Satinsky clamp is simpler,safer and with high success rate.

Dead-in-bed syndrome (DBS) is a rare but devastating sudden death condition that mainly affects young patients with type 1 diabetes.Its mechanism is quite complicated and may involve multiple factors.Acute onset of recurrent and severe nocturnal hypoglycemia,abnormal cardiac repolarization,genetic factors,and obstructive sleep apnea may all contribute to ventricular arrhythmias and even sudden death.The future research plans include elucidating and understanding the underlying mechanisms and triggers for DBS,developing a risk stratification system,using real-time continuous glucose monitoring system to detect severe nocturnal hypoglycemia,and initiating appropriate therapeutic interventions to uhimately prevent the tragedy.