Objective To observe the effect of transcutaneous electrical acupoint stimulation on stress reaction in elderly pa-tients with intravenous patient-controlled analgesia after total hip replacement operation.Methods A total of 60 cases of patients with patient-controlled intravenous analgesia spinal anesthesia after total hip replacement surgery were randomly divided into exper-imental group (n=30)and the control group (n=30).The experimental group respectively at the completion of surgery,postopera-tive 4,24,48 h was treated by transcutaneous electrical acupoint stimulation.The serum cortisol and blood glucose was detected in two groups at preoperative 30 min (T1 ),30 min after operation (T2 ),1 d after operation in the morning (T3 ),2 d after operation (T4 ).The pain scores,sedation scores,adverse reactions and analgesic dosage were investigated in two groups after operation.Re-sults In the experimental group,cortisol and blood glucose levels increased compared T2 and T1 (P <0.01 ).Compared with the control group,cortisol and blood glucose levels in the experimental group decreased at T3 and T4 (P <0.05 ).Compared with the control group,the VAS scores was significantly different in the experimental group(P < 0.01 ).The side effects in experimental group were significantly lower than those in the control group(P <0.05).After TEAS treatment,the physic liquor consumption and press the number decreased significantly (P < 0.05 ).Conclusion TEAS technology for elderly total hip replacement operation PCIA could effectively inhibit stress,enhance the analgesic effect,reduce or avoid the occurrence of adverse reactions.

Objective To analyze the falling events using root cause analysis (RCA) in hospitalized elderly patients in order to supply theoretic reference for precaution of falling events.Methods RCA method was used to analyze the root cause against 62 cases of nursing adverse events of hospitalized elderly patients' falling.Results Hospitalized elderly patients' falling is related to various factors such as body,disease,medicine,psychology,environment and so on.The root cause lied in the elderly' physical function decline,risk factors of external environment,unfulfilled health education,and lack of the care and support system for the elderly,among which cerebral infarction belonging to the disease factors accounted for 22.58％ (14/62),which was the highest;next was senile dementia [20.97％ (13/62)].The rates of agents against psychiatric disorders belonging to the medicine factors and no braking of bed pulley and no use of blister light belonging to environmental factors were the lowest,all were 3.23％ (2/62).Conclusions The falling reason of elderly inpatients can be found out by RCA in order to attain the purpose of improving procedure and prevention in advance.

Objective To investigate the retrieval mechanism of prospective memory,spatiotemporal patterns of event-related potentials (ERP) were used to reveal neural correlates of the prospective interference effect. Methods A task of matching two sequential patterns was taken as the ongoing task and the detection of patterns with two salient levels as prospective memory tasks. Experiments were performed in blocks for single ongoing task block and two prospective memory blocks. Results No significant behavioral effect was found for prospective interference [reaction time(ms):control blocks 750.29±161.33,high salience blocks 770.94±174.89,low salience bocks 764.79±174.12,F(1.52,25.82)=0.48,P =0.57 and correction rate(%):control blocks 85.89±8.07,high salience blocks 88.26±7.00,low salience blocks 87.91±7.28,F(1.50,25.57)=2.46,P =0.12].But significant ERP effect was found at bilateral frontal regions from 160 ms to 240 ms for both two prospective memory blocks. Moreover,greater negativity at the right frontal polar region appeared significantly at 360'440 ms for higher salient patterns. Conclusion Prospective memory is retrieved based on strategic monitoring and high salient stimuli may enhance to keep the prospective goal and improve task switching.

Objective To investigate neural correlates of directed forgetting. Methods ERP was recorded from 12 healthy postgraduates while they performed a directed forgetting task based on the item-cueing method. During the study phase, each word was followed by a cue indicated that it need to be remembered, to be forgotten or just to be viewed. During the test phase, subjects were asked to make an old/new recognition response. One-way ANOVA of repeated-measurement and paired comparisons were carried out for the cue factor. Results Both of the accuracy and reaction time of the TBR group were significantly superior to those of the other two groups. No significant behavioral effect was found between the TBF group and the TBV group. In the study phase, statistical parametric mapping of ERP suggested the cue effects were presented in the frontal poles ( 200～300 ms ) and the parietal regions (300～400 ms) ,the left fronto-parietal and the right occipital region (450～700 ms). The difference between the TBF and the TBV was significant in the left parietal area (500～525 ms) and has the significant trend in the right frontal area ( 350～600 ms). In the retrieval phase, the old/new effects of ERP occurred in the left fro-nto-temporal (300～450 ms) for the TBF cue. Conclusions The TBF cue and the TBV cue have the equivalent behavioral performance. During the study period, the activation of the right frontal lobe and the left parietal suggests the active inhibition evoked by the TBF cue. And the TBR cue can enhance the rehearsal function of left hemisphere and improve the memory performance of words. The retrieval of the items of TBF is based on more familiarity and less recollection.

ObjectiveTo investigate the point prevalence rate of nosocomial infection and discover the problems in the management of nosocomial infection.MethodsA team of surveyors were trained to collect necessary data of inpatients on a single day. Questionnaires about nosocomial infection cases were filled out and analysed.Results492 patients were investigated, 31 patients developed into nosocomial infection, the point prevalence rate was 6.30%. The point prevalence rate of nosocomial infection in ICU was 57%; The main infectious site was urinary tract. ConclusionThe survey on the point prevalence rate is beneficial to manage nosocomial infection.

With the development of the quality of life, the morbidity of hyperuricemia is increasing year by year. At the same time, it appears that this disease attacks the young people currently. As the study of pathogenesis of hyperuricemia advanced, a series of uric acid transporters were found during this process. Meanwhile, the definition of transporterome was proposed. They were divided into three groups according to the functions: reabsorption proteins, excretion proteins and skeleton proteins. At moment, the drugs for hyperuricmia mainly include uric acid composition inhibitors and uric acid excretion promoters. Since the excretion of uric acid plays a leading role during the process of attack of hyperurecimia, it makes sense to explore Chinese medicines with clear mechanism targeting the transporterome. Therefore, this paper would focus on transporterome and summarize the mechanisms of Chinese medicines in treating hyperuricemia.
Animals ; Biological Transport ; Carrier Proteins ; antagonists & inhibitors ; genetics ; metabolism ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hyperuricemia ; drug therapy ; metabolism ; Uric Acid ; metabolism

Asian Continental Ancestry Group ; Databases as Topic ; Humans ; Injury Severity Score ; Maxillofacial Injuries

BACKGROUND:Bone marrow mesenchymal stem cells have beneficial effects on the treatment of various diseases. Mitochondria transfer is newly proposed and its specific mechanisms of action and control factors remain unclear.
OBJECTIVE:To review the studies about stem cells and mitochondrial transfer, then to discuss its value on clinical.
METHODS:The PubMed, VIP and Wanfang databases were searched for related articles concerning stem cells and mitochondrial transfer. Key words were“stem cell, embryonic stem cell, progenitor cell, mitochondria”in Chinese and“Stem cell[s], Mother cell[s], Progenitor cell[s], Colony-Forming Unit[s], Colony Forming Unit[s], Mitochondria[l] transfer”in English. Thirteen articles were found initial y, and using the citation tracking method, final y 42 articles were determined. RESULTS AND CONCLUSION:Studies have shown that mitochondrial transfer from bone marrow mesenchymal stem cells is associated with rescue of aerobic respiration and restoration of mitochondrial function in the injured somatic cells. Bone marrow mesenchymal stem cells and recipient cells form tunneling nanotubes for mitochondrial transfer. Movement of mitochondria between cells is regulated and directed by Miro1. The successful transfer of mitochondria may be accompanied with the clearance of damaged mitochondria. The rescue of mitochondrial function in early stages may provide a valuable therapeutic strategy for various diseases include acute lung injury.

Aim To study the effects of luteolin on the expression of TGF-?1 mRNA in rats with pulmonary fibrosis, and elaborate the molecular mechanism of luteolin in pulmonary fibrosis therapy. Methods The model of pulmonary fibrosis was established through instilling bleomycin intratracheally. After luteolin treatment, the pulmonary index and the content of hydroxyproline (HYP) were observed. The levels of TGF-?1 mRNA in pulmonary tissues were determined with RT-PCR. Results The pulmonary index and the content of HYP decreased markedly in pulmonary fibrosis rats treated with luteolin. Furthermore, luteolin inhibited evidently the expression of TGF-?1 mRNA in lung tissues with pulmonary fibrosis. Conclusion The molecular mechanism of luteolin in pulmonary fibrosis therapy was associated with inhibition of the expression of TGF-?1 mRNA.

With the aggravation of enviromental pollution, a quickly, sensitive, inexpensive monitoring means should be developed to protect water resources. Luminescent bacteria as biosensor have the advantages mentioned above. During past two decades, luminescent bacteria toxicity test was used widely in comprehensive toxicity evaluation, screening, monitoring and specific pollutants detection in water, the recent progress in this field was reviewed in this paper.