This paper summarized the nursing care of 6 patients with severe aplastic anemia complicated with DIC. The risk of sepsis and severe pneumonia can be reduced through actively nursing and preventing of oral,res-piratory,skin and perianal infections. The chances of bleeding from the skin,nose,and mouth can be reduced by less skin puncture,local medication,cold compresses and compression. Also by monitoring the coagulation indexes, estimating the phase of DIC and observing the bleeding manifestations. Through controlling heparin dripping rate, monitoring coagulation index,reporting to doctors at any time and knowing how to deal with heparin overdose,can increase the safety of heparin administration and improve the clinical application rate of early DIC heparin therapy. Psychological guidance can reduce the risk of cerebral hemorrhage caused by excessive exertion and accidental fall. After treatment and careful nursing,6 patients improved,5 cases were discharged,and 1 case died.

Objectives:To study the expression of PTEN in endometrial carcinoma under different pathological factors and to discuss its relationshipwith the occurrence of endometrial carcinoma and the clinical significance.Methods:The expressions of PTEN in 40 cases of endometrial carcinoma,10 cases of atypical hyperplasia and 10 cases of normal endometria were examined by immunohistochemical method.Result:The positive staining rate of PTEN in endometrial carcinoma was 55.00%,and that in normal endometria was 100.00%.There was no obvious correlation between the expression of PTEN in endometrial carcinoma and different pathological factors such as clinical staging,depth of myometrial invasion and lymph node metastasis.Conclusion:The expression lack of PTEN exists in endometrial carcinoma,and PTEN has an important role in the occurrence of endometrial carcinoma.

The software design of a biological enzyme calibration system based on virtual instrument technology is introduced. The serial communication with LabVIEW and data tele -distribution with DataSocket technology are also described.

Objective To explore the relationship between mindfulness and rumination of nursing students in clinical practice. Methods Three hundred and sixty two nursing students participated in the survey using Chinese version of mindfulness scale and rumination scale.Results The scores on mindfulness and rumination among the 362 nursing students were 3?18 ± 0?37 and 1?62 ± 0?19,respectively.The mindfulness among them was negatively correlated with their rumination and so it were with the three dimension of mindfulness:awareness in action,non-reactiveness of inner experience and non-judgment on inner experience with the 3 dimensions of rumination(P<0?01 or P<0?05).The 3 dimensions of mindfulness predicted the variance of rumination by 31?1%.Conclusions The mindfulness of nursing students is at moderate level,and their rumination at low level.Therefore,the nursing administrations or educators should further cultivate mindfulness of nursing students to reduce the extent of rumination?

The bilingual teaching is a new mode to enhance the synthetic ability of the medical students' foreign languages.The article proposes several manners of guarantying the teaching effect such as strengthening the teacher's quality,applying multimedia to teaching,using unified teaching material and increasing students' interest in learning.

Objective To explore the psychological intervention on the anxiety of patients under gastroscope examination. Methods According to the self-rating Anxiety Scale (SAS), 100 patients with anxiety disorder were randomly divided into observation group 50 cases, a control group of 50 cases, check the two groups of patients before, during and after adopt routine health education, observation group given psychological intervention at the same time, compared the two groups of patients with anxiety, check the time and satisfaction. Results After the psychological intervention, the scores of self-rating anxiety scale of observation group were significantly lower than those of control group, there were signiifcant differences (P<0.05), check time was shortened obviously, the difference was statistically signiifcant (P<0.05), satisfaction was also signiifcantly better than the control group the difference was statistically significant (P <0.05). Conclusion Psychological intervention can reduce the anxiety level of patients with gastroscope, shorten the examination time of gastroscope, increase the ability of patients to understand the disease, improve patient satisfaction.

In order to improve the performance of the photorefraction device,shorten the detection time and improve the accuracy of measuring,the theory of a new kind of double-cranny eccentric photorefraction device is described.Some features and key point of technology are emphatically pointed out.A method is put forward to determine the ocular diopter by image processing.

This paper presents a new design method and basic principle for the data acquisition system of the biochemical analyzer. The hardware, software configuration and principle of A/D converter module are also introduced. Field experiment shows that the system is effective and can satisfy related accuracy specification.

Objective To investigate the relationship between PrfA-dependent promoters and PrfA regulation. Methods LacZ reporter gene fusions used to investigate the inhibitory elements for PrfA-dependent transcription were carried on two promoters of Listeria monocytogenes: a PrfA-dependent promoter of the phospholipase gene pica (PplcA) and a putative promoter of the aroA gene (ParoA2) which contains a similar PrfA-binding site (PrfA-box) and a similar-10 box as PplcA but does not function as PrfA-dependent promoter. A series of hybrid plcA-aroA promoters by exchanging corresponding sequence elements of these two "promoters" were constructed and incorporated into upstream of a promoterless lacZ gene. The variant promoter-lacZ transcriptional fusions were then electroporated into L. monocytogenes wild-type strain P14, prfA mutant P14a and prfA deletion mutant A42, respectively. The expression level of PrfA is the highest in the P14a and the lowest in A42. The corresponding transcription activities of hybrid promoters were measured by the β-galactosidase assay. Results The two critical elements of PrfA-dependent promoters, the PrfA-box and the-10 box, can be functionally exchanged as long as the distance in between is maintained 22 or 23 bp. However, the interspace sequence and the sequence downstream of the -10 box of ParoA2 were strongly inhibitory for PrfA-dependent transcription. Conclusion Downstream sequence together -10 box of ParoA2 might fold into a hairpin structure when present in a single stranded DNA and possibly block the formation of the transcriptional initiation open complex, hence, inhibit the PrfA-dependent transcription from ParoA2.

Objective To investigate the effect of seasonal variation of blood pressure (BP) on the incidence of cardiovascular and cerebrovascular events in the elderly aged 80 years and over.Methods The 67 patients (aged 80-86 years) with essential hypertension were enrolled, the BP were measured at home, in consulting room, and by 24-h ambulatory BP monitor in every season for 2 years. The incidences of cardiovascular and cerebrovascular events were recorded in the meantime.Results Both systolic BP (SBP) and diastolic BP (DBP) were lower in summer than in any other season (P＜0. 01 ). SBP was higher in winter than in any other season. (P＜0.01), DBP was higher in winter than in summer(P＜0. 01). There were no significant differences in SBP and DBP between spring and autumn. The incidence of cardiovascular and cerebrovascular events were higher in winter and summer than in spring and autumn (P＜0.05). The season (OR= 1. 525, P=0. 001), BMI (OR =1.145, P=0.018) and heart function (OR= 2. 037, P=0.01) were related to the incidence of cardiovascular events. And the season (OR = 1. 604, P= 0. 001 ), history of cerebrovascular disease (OR= 1. 598, P=0. 034), office-measured SBP (OR=0.960, P=0.013) and ambulatory DBP (OR =0. 936, P = 0. 008) were related to the incidence of cerebrovascular events.Conclusions The blood pressure measured by three methods in the elderly aged 80 and over shows that both SBP and DBP are at the lowest in summer; SBP is at the highest in winter, DBP is higher in winter than in summer. There are no significant differences in BP between spring and autumn. The incidences of cardiovascular and cerebrovascular events are significantly higher in winter and summer than in spring and autumn.