Background:Chronic atrophic gastritis( CAG)is a common type of gastritis and is closely related to the pathogenesis of gastric carcinoma,however,the efficacy of its drug treatment is not clear. Aims:To study the therapeutic effect of capsaicin on CAG in rats. Methods:CAG rat model was established by intragastric administration of sodium deoxycholate, ethanol and ammonium hydroxide. Forty-four survival model rats were randomly divided into CAG model group and low, moderate,high dose capsaicin groups,rats in the latter 3 groups were intragastrically administrated with 1 mg·kg-1 ·d-1 , 10 mg·kg-1 · d-1 ,30 mg · kg-1 · d-1 capsaicin,respectively,for 28 days. The inflammation and degree of atrophy of gastric mucosa were observed by HE staining. Gastric mucosal levels of pepsinogen( PG)Ⅰ,PGⅡ were determined by ELISA. The protein expressions of IKKβ,NF-κB,XIAP were determined by Western blotting. Results:The differences in inflammation and degree of atrophy of gastric mucosa,levels of PGⅠ,PGⅡ,protein expressions of IKKβ,NF-κB,XIAP were statistically significant among model group,low,moderate,high dose capsaicin groups( P <0. 05 ). With the increasing of capsaicin dose,inflammation and degree of atrophy of gastric mucosa were improved in a dose-dependent manner(P<0. 05),protein expressions of IKKβ,NF-κB,XIAP were decreased. Conclusions:Capsaicin is effective for the treatment of CAG.

Objective To explore the mutual effect of proinflammatory factor tumor necrosis factor(TNF)-? and cigarette smoke extract(CSE) in the induction of mucin 5AC in BEAS-2B human airway epithelial cells.MethodsThe BEAS-2B cells were transfected with nuclear factor(NF)-?B decoy oligonucleotides(ODNs) or transfected with NF-?B scrambled ODNs as negative control,then treated with TNF-?,CSE,and TNF-? plus CSE,respectively. The expression of phosphorylated epidermal growth factor receptor(p-EGFR),mucin 5AC(MUC5AC) protein production and gene expression were detected by Western blot,ELISA and RT-PCR respectively.Results Significant increasing of p-EGFR protein production in scrambled ODNs transfected cells with elevation of MUC5AC protein production and mRNA expression was recorded and TNF-? and CSE co-incubated groups were much higher than single TNF-? or CSE stimulated group(P

Objective To evaluate clinical value of tension-free hernia repair in inguinal hernia.Methods The clinical data of 181 patients with 188 inguinal hernia were treated with tension-flee hernia repair,Mesh plug-patch were used in the operations.The operative procedure,postoperative pain,patient ability recovery,complications and recurrence rate were studied.Results All cases operated successfully.The mean operation time was 50min.The postoperative complications were 6 cases of urinary retention and 3 cases of scrotal hydroceles.Local hematoma appeared in 5 cases.3 cases complained of foreign body feeling.The average postoperative stay was 7 days.The follow-up period ranged from 3 to 36 months and no recurrence was observed.Conclusions Tension-free hernia repair is a perfect surgical operation for the inguinal hernia.It has many advantages,such as simplicity,minimal invasion,less postoperative pain,rapid recovery,good recent therapeutic effect and lower recurrence.The operation type should be individulized.

Objective To evaluate the efficacy of recombinant human interferon α-2b gel onflooding prevention in loop electrosurgical excision procedure (LEEP) during and after the operation.Method The 152 patients with cervical disease were randomly divided into three groups: group A(recombinant human interferon α-2b gel treatment group), group B (hypophysin treatment group)and groupC(control group). Results The amount of intraoperative bleeding and the amount of post-operative bleedingof the group A was (2.57±1.19) ml and (2.24±1.75) ml, the group B was (3.00±1.49) ml and (45.86±26.14) hal, the group C was (45.62±39.57) ml and (56.90±41.90) ml, respectively. There was no differencebetween group A and group B in the amount of intraoperative bleeding (P>0.05), but significantly less thangroup C (P<0.01). There was no difference between group B and group C in the amount of post-operativebleeding (P>0.05), but significantly much more than group A (P<0.01). Conclusion Recombinanthuman interferon α-2b gel is effective on reducing intraoperative and post-operative bleeding in LEEP.

DNA methylation is one of the regulators on the expression of genes,and the expression of genes can be ”silenced” by hypermethylation of the tumor suppressor genes,which is in close relationship with carcinogenesis.Gastric cancer is caused by multiple factors and multiple genes alteration and prolonged interaction.among them ,There is considerable relation between the hypermethylation of the tumor suppressor genes and the gastric carcinogenesis.

Potential toxicities of three classes of dyes and pigments were introduced in this review. Some azo/benzidine dyes, anthraquinone dyes and triarylmethane dyes have carcinogenicity,some azo/benzidine dyes and anthraquinone dyes may cause skin irritation, some noncarcinogenic triarylmethane dyes have risk of serious damage to eyes and may exert long term adverse effects on the aquatic environment.

Objective To study the mechanism of reinforcing kidney and activating blood therapy for the apoptosis of endometriosis.Methods Female SD rats aged 8~12weeks were grouped as model group,western medicine group,Chinese medicine group and integrated Chinese and western medicine group,with 15 in each group.The light microscope,electron microscope,TUNEL method,and immunohistochemical method were adopted to observe the pathomorphology,positive rate of apoptotic cells and the positive expression of apoptosis-regulating gene bcl-2 and bax.Results The apoptosis of endometriosis tissues in the integrated Chinese and western medicine group was the most obvious result with the highest apoptosis index,and the positive expression of apoptosis regulating gene bcl-2 was downward regulated and that of apoptosis regulating gene bax upward regulated,so bcl-2/bax decreased.Then it was Chinese medicine group,western medicine group,and model group.Conclusion The occurrence of endometriosis is related with the lowering of ability of apoptotic cells.Chinese medicines with the functions to reinforce kidney and activate blood can promote the ability of apoptotic cells in endometriosis to quicken the atrophy and regression of endometriosis tissues.

Objective This single-center prospective,case-controlled study was carried out to investigate clinical effects on patients with aging face who underwent facial autologous fat grafting with platelet-rich plasma (PRP).Methods Thirty patients with facial sagging or partial depression and requiring autologous fat grafting were randomly selected for the study.Photograph and 3D scanning were taken before and 3-month after the operation.Patient and physician satisfaction was rated using Visual Analogue Scale (VAS) ranging from 1 (most unsatisfactory) to 10 (most satisfactory)3 months after the operation.Statistical difference between the patient and physician satisfactory scores was analyzed.Results The average physician VAS score was 7.9 ± 1.0 while the average patient VAS score was 8.0±1.2.The scores between two groups had no significant difference (P＞ 0.05).Four patients developed bruising and swelling postoperatively that disappeared less than 7 days later.There was no feed back of serious complications from patients.Further investigation of patients with scores less than 7 showed the main reason of low patient satisfaction was that the improvement of facial depression did not meet the requirements of the patient,and fat absorption led to unsatisfactory filling.Conclusions The high satisfactory scores indicate good aesthetic result of facial autologous fat grafting with PRP.PRP may lead to higer survival rate of fat and satisfaction.

Objective To explore the mutual effect of proinflammatory factor tumor necrosis factor (TNF)-α and cigarette smoke extract(CSE) in the induction of mucin 5AC in BEAS-2B human airway epithelial cells. Methods The BEAS-2B cells were transfected with nuclear factor(NF)-κB decoy oligonucleotides(ODNs) or transfected with NF-κB scrambled ODNs as negative control,then treated with TNF-α,CSE,and TNF-α plus CSE, respectively. The expression of phosphorylated epidermal growth factor receptor (p-EGFR) , mucin 5AC( MUC5AC) protein produc-tion and gene expression were detected by Western blot, ELISA and RT-PCR respectively. Results Significant in-creasing of p-EGFR protein production in scrambled ODNs transfected cells with elevation of MUC5AC protein production and mRNA expression was recorded and TNF-α and CSE co-incubated groups were much higher than single TNF-α or CSE stimulated group(P <0. 05). In NF-κB decoy ODNs transfected cells,TNF-α stimuli revealed an obvious reduction of MUC5AC protein production and gene expression. Conclusion TNF-α and CSE can syner-gistically induce mucin 5AC expression in BEAS-2B cells, transcription factor NF-κB plays a critical role in TNF-α induced mucin production but not as so important in mucin production by CSE stimulated cells.

MicroRNAs(miRNAs) are a type of highly conserved,small non-coding RNAs,which powerfully regulate gene expression at the posttranscriptional level.In humans,it modulates about 30％ of protein coding genes.Ocular neovascularization is one of the most important clinical problems in ophthalmology and a common cause of blindness.Recent studieshavedemonstrated that miRNAs play a crucial rolein the development of ocular neovascularization.The use of miRNAs provides novel therapeutic approaches for ocular neovascularization.Here,we describe our understanding of the functions and expressions of miRNAs in eyes,the relationship among miRNAs,ocular neovascularization and angiogenic factors,and the current research of miRNAs in diabetic retinopathy.