OBJECTIVE:To provide references for drugstores to launch pharmaceutical care.METHODS:The consents,methods and obstacles in launching pharmaceutical care for drugstores were analyzed.RESULTS&CONCLUSION:The launching of pharmaceutical care in drugstores can not only considerably ensure the benefits of consumers in drug use,but also raise their own core competitive power.

OBJECTIVE: To establish an analytical method of ultra performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) for identification and quantitation of the multi-constituents of Danshen-chuanxiongqin injection. METHODS: The method of UHPLC-HRMS was developed to identify the complex chemical composition and full ms scan mode was used to determine the main active ingredients precisely, then the means of principal component analysis (PCA) was used to comprehensively assess the quality of Danshen-chuanxiongqin injection. RESULTS: A total of 23 chemical constituents were identified in Danshen-chuanxiongqin injection, 14 of which were unambiguously identified by comparing with chemical standards, and seven major compounds, ligustrazine, danshensu, salvianolic acid A, succinic acid, rosmarinic acid, protocatechuic aldehyde, caffeic acid, in Danshen-chuanxiongqin Injection were determined accurately. The analysis by multivariate data processing software SIMCA 14.0 indicated that the quality of most batches of samples were stable except ligustrazine, danshensu, and salvianolic acid A which were a bit unstable in some individual batches, and it was essential to monitor the content of salvianolic acid A to control the drug quality. CONCLUSION: The established HPLC-TOF-MS/MS method has the properties of rapid identification, high specificity, simpliness, and feasibility, which can be used as a better means for the qualitative and quantitative analysis of danshen-chuanxiongqin injection. Moreover, it lays the ground for quality control of the drug and provides data reference for the rational use in clinic.

Arsenic ; urine ; Chromatography, High Pressure Liquid ; methods ; Formamides ; analysis ; Humans ; Urinalysis ; methods

Objective To compare the therapeutic results of laparoscopic and open treatment for choledocholithiasis. Methods A prospective comparison was conducted between 29 cases of laparoscopic choledocholithotomy and 35 cases of open choledocholithotomy in terms of stone removal,operative time,hospitalization day,hospitalization cost and postoperative complications. Results Stone removal was achieved in 28/29 patients by a laparoscopic approach and in 32/35 patients by a open approach( ? 2=0 71, P =0 399);average operative time was (178 3?32 4) min in laparoscopic approach and (145 8?27 1)min in open approach( t =4 27, P =0 01);the patients in laparoscopic procedures had flatus in (20 8?2 9) hour and those in open procedures did in (58 2?4 9) hours( t =37 8, P 0 05);no major complication was found in laparoscopic approach. Conclusions Laparoscopic choledocholithotomy is safe and effective and it is superier to open treatment.

Objective To investigate the feasibility and techniques of laparoscopic biliary re-operations. Methods Laparoscopic surgery was performed in 13 patients with bile duct stones who already had undergone biliary tract operations. Results Laparoscopic choledochotomy was successfully completed in 12 cases, while a conversion to open surgery was required in 1 case. The success rate of laparoscopic surgery was 92.3% (12/13). The operation time was (101～300) min, with a mean of 155 min. No intra- or post- operative complications occurred. Conclusions Laparoscopic biliary re-operations are feasible and experiences are needed to ensure the success of surgery.

Objective: To investigate the efficacy of gemcitabine in chemotherapy of cervical carcinoma. Methods:10 untreated cervical carcinoma patients had been divided into two groups,5 cases were treated with neoadjuvant chemotherapy and radiotherapy,others were treated only with radiotherapy. All patients were operated and compared the pathologic changes of cancers. Moreover 3 cases with recurrent tumors had been treated with gemcitabine and other agents . Results: The rate of complete response(CR) was 60% in group of neoadjuvant chemotherapy and 20% in patients with only radiotherapy. Only one case achieved partial response (PR) in patients with recurrence. Conclusion: The neoadjuvant chemotherapy in cervical carcinoma with gemcitabine is helpful for achieving good response .As radiation sensitizer or radio-potentiator,gemcitabine can promote efficacy of neoadjuvant chemotherapy in cervical carcinoma.

Objective: To study the factors relating the prognosis of ovarian granulosa cell tumor and the characteristics of its clinical presentations. Methods: The clinical records and pathological sections of 24 cases with granulosa cell tumor of ovary were reviewed retrospectively. The clinical characteristics, the clinical stage, and the therapeutic programs and the results were summarized respectively. Results:The 5-years survival rate showed that the clinical stage influenced the prognosis of ovarian granulosa cell tumor obviously. Conclusion: The early dection is the major factor which influences the patients' survival rate.

Objective To explore the chemotherapeutic agents-induced modulating effects of Egr-1 promoter sequence on GM-CSF expression and its protective effect against injury to haematopoiesis due to chemotherapy.Methods Human GM-CSF cDNA and enhanced green fluorescent protein(EGFP) cDNA were linked to internal ribosome entry site(IRES) respectively,and then recombined to pCIneo vector containing Egr-1 promoter(Egr-EG).The vector was transferred into human bone marrow stromal cell line HFCL by lipofection,and the HFCL/EG cells were then finally constructed.MTT assay was performed to determine the effects of cisplatin(DDP),5-fluorouracil(5-FU),gemcitabine(GEM) and paclitaxel(PTX) on the survival rates of HFCL/EG and HFCL cells,and the IC50 of such agents against HFCL/EG and HFCL cells were calculated.The percentage of HFCL/EG cells which positively expressed EGFP was assessed by both flow cytometry and inverted fluorescence microscope.The effects of the active oxygen inhibitor N-acetylcysteine(NAC) on the expression of GM-CSF,which was modulated by promotor Egr-1,were detected by ELISA.The effects of HFCL/EG supernatant on CFU-GM after being exposed to chemotherapeutic agents were examined.Results With different sensitivity to DDP,5-FU,GEM and PTX,HFCL/EG cells were successfully constructed.The drugs showed higher IC50 value against HFCL/EG cells than HFCL cells(P

Through a congenital cleft hand cleft foot line analysis of clinical manifestations, the mode of inherit-ance, clinical type of 15 patients. It was found that the pedigrees of congenital foot deformity in patients with cleft hand cleft hand split foot crack were typical, and non-syndromic. The disease presents the typical pedigree body autosomal dominant mode of inheritance, clinical manifestations of the great differences between patients, the pres-ence of significant genetic heterogeneity.