The late-preterm infant refers to the infant who is born at 340/7 through 366/7 week's gestation.In recent years,the number of the late-preterm continually ascends and the proportion of them in the preterm group increase as well.Recent studies find that the rates of morbidity and mortality of late-preterm infants are much higher than those of term infants.The rise of the proportion of the former and the more medical resources consumed attract the considerable attention thereby.In this article,we will review late-preterm infants' morbidity of several systems and its influential factors in order to provide more information for perinatal medical staff to improve diagnosis,treatment and further research.

OBJECTIVE:To establish a method for content determination of dihydroartemisinin in Dihydroartemisinin and piper-aquine phosphate tablets. METHODS:HPLC was performed on the column of Waters YMC-Pack ODS-AQ with mobile phase of acetonitrile-water(gradient elution)at the flow rate of 1.0 ml/min,the detection wavelength was 216 nm,column temperature was 30℃,and injection volume was 20 μl. RESULTS:The linear range of dihydroartemisinin was 0.009 650-1.930 mg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.5%;average recovery was 100.76%(RSD=0.95%,n=9). CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the content determination of dihydroartemis-inin in Dihydroartemisinin and piperaquine phosphate tablets.

The formation and progression of tumor is inseparable from the changes of cell cycle regulatory proteins.The G2-M period block effect of tumor cells is especially important in slowing the growth of tumor and improving the curative effect.DNA damage receptor,signal transduction factor and effectors are the classical pathways to mediate G2 phase arrest of tumor cells,and have the potential to be new targets for tumor therapy.

The standardized patient (SP) has been widely used in medical education and clinical training. The teacher-standardized patient (TSP) is played by health providers or medical educators, taking roles as patient, evaluator and teaching guider. The consultation ability of general practitioners includes two aspects: the clinical competency and doctor-patient communication skill. This article reviews the application of TSP in evaluation of consultation ability of general practitioners, focusing on the recruit, training, assessment and application of teacher-standardized patients.

As a new emerging infectious virus, 2019 novel coronavirus (2019-nCoV) has been widely popular all over the world since the beginning of 2020. As a special group, children have similarities and differences with adults for coronavirus disease 2019 (COVID-19) in the epidemiology, diagnosis and treatment, curative effect monitoring, prognosis, virus testing methodology, infection and inflammation markers, etc. This paper mainly reviews the characteristics of COVID-19 in children from two aspects: the epidemiological and pathogenic detection characteristics, so as to provide some basis for its diagnosis and treatment.

Procalcitonin ( PCT) has closely related to the severity of senile pulmonary infection, and its dynamic detection can be used as the auxiliary diagnosis index for elderly severe pneumonia ( SP) . The effects of serum PCT dynamic detection in the early quick identification, diagnosis, disease course monitoring and prognosis evaluation for elderly SP were reviewed, and the effects and clinical significance of the detection in the optimization of antibiotic therapy were also summarized in the paper. The results showed the impor-tant value of PCT dynamic detection in the diagnosis of elderly SP and anti-infection therapy.

Objective To investigate the expression and significance of hypoxia-inducible factor 1 alpha (HIF- 1α), vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in tissues of condy- loma acuminatum (CA) in pregnant women. Methods Tissue specimens were obtained from the lesions of 30 pregnant women with CA and 30 non-pregnant women with CA, and from the vulva of 15 normal preg- nant women. By immunohistochemical staining, the expressions of HIF -1α, VEGF and Ang-2 were detected in these specimens. Results The expression rates of HIF -1α,VEGF and Ang-2 were 86.67%, 93.33% and 83.33% in pregnant women with CA, respectively, 63.33%, 66.67% and 53.33% in non-pregnant women with CA, respectively, and 0, 6.67% and 0 in normal women, respectively. Enhanced expressions of HIF -1α,VEGF and Ang-2 were observed in pregnant women with CA compared with the latter two groups (P < 0.05). In pregnant women with CA, a significant correlation was noted between the expression of HIF -1α and VEGF (r = 0.412, P < 0.01) and between the expression of Ang-2 and VEGF (r = 0.460, P < 0.01). Further more, the expression of HIF -1α, VEGF and Ang-2 positively correlated with each other in non-preg- nant women with CA. Conclusion In tissues of CA in pregnant women, HIF-1α,Ang-2 and VEGF are over expressed, which may be related to angiogenesis.

The technology,of extracting Semen Cassiae from Jueming Jiangzhi Powder was investigated.The perceutages of extra ction and preservation of anthraquinone were used to select the methods of extra ction, concentration and drying of Semen Cassiae. The optimal procedure was set as follows:the large-size powder of Semen Cassiae was extracted with 50% alcohol for three times, then the extracted liquid was concentrated by vacuum evaporati on and finally dried by spraying.

Cell culture and SDS-polyacrylamide gel electrophoresis (PAGE) methods were employed to analyse the role of human amniotic basement membrane (HABM) in supporting the growth of forebrain neurons and the components of HABM after enzymatic digestion. It was found that HABM treated with heparinase Ⅱ supported the growth of forebrain neurons 150% of that of the untreated HABM control. The results of SDS-PAGE analysis showed that HABM treated with heparinase Ⅱ had collagen-like polypeptides 2?_1 (260kD) and 2?_2 (225kD) reduced and lamininlike peptides (212kD and 200kD) increased; and that HABM treated with heparinase I or collagenase I had laminin reduced. This indicated that laminin is the major component in HABM to promote the growth of forebrain neurons.

DNA methylation is one of the regulators on the expression of genes,and the expression of genes can be ”silenced” by hypermethylation of the tumor suppressor genes,which is in close relationship with carcinogenesis.Gastric cancer is caused by multiple factors and multiple genes alteration and prolonged interaction.among them ,There is considerable relation between the hypermethylation of the tumor suppressor genes and the gastric carcinogenesis.