With the improvement of cataract operation, the cataract surgery has become increasingly perfect. The cataract patients show greater expectation for the result of cataract operation. As a result, refractive cataract surgery has become the main trend. Detailed investigations of corneal diseases, lens density, corneal topography, preferable intraocular lens ( IOL ) choice, and IOL power calculation can help us get a better knowledge of preoperative conditions on patients, which can be conducted with pentacam. So we can have a better forecast of post - operative outcome and improve the quality of vision for cataract patients after surgery.

Humans ; Sympathectomy ; adverse effects ; methods ; Thoracoscopy ; adverse effects ; methods

Objective] Making a discussion on academic features ofYi Lin Kou Pu Liu Zhi Mi Shu. [Method] With the research methods of philology, we collect Qing transcript ofYi Lin Kou Pu Liu Zhi Mi Shuand analyze its academic features. [Result]Yi Lin Kou Pu Liu Zhi Mi Shuwas additionally compiled, supplemented and annotated by Zhou Sheng, a famous doctor of the Qing Dynasty, on the base of Yi Lin Kou Pu which was composed by Lu Qi. Within four volumes, the contents of the book contain Medicine, Surgery, Gynecology, Pediatrics, etc. The diagnosis and treatment based on syndrome differentiation and Chinese herbal prescription in this book have their own characteristics. That is: theories from different schools make arguments on the facts; six treatments of syndrome lucidly expose the essential points; secret prescriptions and Chinese herbs are widely collected; opinions and knowledge from his teacher grasp the essence of the problem and bring out the crucial point. [Conclusion]Yi Lin Kou Pu Liu Zhi Mi Shu is a distinctive and only existing medical book with high academic value and practical significance.

In the clinical skill evaluation,SP and the new technology OSCE for medical educational evaluation brought by SP can offer a vivid and complete exam atmosphere for the medical students,which have been widely applied by medical colleges and schools at home and abroad,with real,effective,reliable characteristics and broader development prospect. To induce the technology into TCM clinical evaluation is in accordance with the requirements of TCM educational development,which can make students better grasp clinical various basic skills within limited studying periods.

objective: To observe the distribution of pegylated liposmal doxorubicin(PLD) in animal model of tongue cancer. Methods: Tongue cancer model was established in 40 golden hamster, PLD or free doxorubicin at the dose of 9 mg/kg was injected perifocally into each of 20 mice, the concentration of the drugs in lypmph node and blood was measured with HPLC. Results: After injection of free ADM peak concerntration in blood was acheived in 3 h and in lymph nodes in 1 h, while after injection of PLD, that was in 16 and 6 h respectively. The concertration of PLD was heigher than that of ADM in both blood and lymph node from 16 or 6 h till 216 h after injetion.Conclusion: PLD can be regarded as valuable drug delivery system in the treatment of oral cancer.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Facial Injuries ; therapy ; Female ; Humans ; Male ; Middle Aged ; Neck Injuries ; therapy ; Retrospective Studies ; Wounds, Penetrating ; therapy ; Young Adult

Objective To compare the therapeutic efficacy between laparoscopic peritoneal vaginoplasty and sigmoid loop vaginoplasty. Methods A total of 29 cases of congenital absence of vagina received either laparoscopic peritoneal vaginoplasty (Peritoneal Group, 14 cases) or sigmoid loop vaginoplasty (Sigmoid Group, 15 cases) from March 1998 to April 2003. Results The operation failed in 1 case in the Peritoneal Group, requiring a conversion to open sigmoid loop vaginoplasty. Both groups had no surgical complications or postoperative pyrexia. The operating time of the Peritoneal Group (118 9?19 0 min) was significantly shorter than that of the Sigmoid Group (202 0?18 6 min) ( t =-11 674, P =0 000). And the intraoperative blood loss of the former (36 2?12 6 ml) was significantly less than that of the latter (105 3?46 3 ml) ( t =-5 205, P =0 000). The conditions of reconstructed vagina of both groups were basically the same, but the vagina length of the Sigmoid Group (10 8?1 1 cm) was significantly greater than that of the Peritoneal Group (9 0?0 8 cm) ( t =-4 882, P =0 000). Among the Sigmoid Group, 3 cases had somewhat more amount of vaginal secretion and 4 cases had abnormal-smelling secretion. Patients in both groups reported the same satisfactory degree of sexual life. Conclusions Laparoscopic peritoneal vaginoplasty and sigmoid loop vaginoplasty present a similar therapeutic efficacy, but the former gives less surgical invasion than the latter.

[ABSTRACT]OBJECTIVETo investigate the cytomegalovirus infection in neonates, characteristics of gap junction protein Connexin26 gene mutation and the hearing follow-up results, and to analyze their correlations. METHODS60 CMV-DNA positive and 40 CMV-DNA negative neonatal newborn from The Second Affiliated Hospital of Wenzhou Medical University and The first people's Hospital of Yongkang were screened, the blood biochemistry was analyzed, and the umbilical cord blood was reserved to detect the Connexin26 gene expression of mRNA with RT-PCR.PCR results was sequenced to track the newborn hearing, and analyze the correlations between neonatal cytomegalovirus types, the mutation of Connexin26 gene and hearing test results.RESULTS 26 cases from 60 CMV-DNA positive newborns were found with blood biochemical abnormalities. In all of the newborns, a total of 41 cases had 235delC mutation, 11 cases in the mutations for the development of hearing impairment. The results of correlation analysis showed that there were correlations between cytomegalovirus infection, gene mutation and hearing impairment.CONCLUSION Cytomegalovirus infection in neonates can lead to mutations in the Connexin26 gene, and may further lead to hearing impairment, and the probability of the mutation of Connexin26 gene and sensorineural hearing loss were higher in symptomatic cytomegalovirus infection neonates.

B cell maturation antigen (BCMA) is a receptor of B lymphocyte stimulator (BLyS). Human IgG1Fc fusion proteins with the extracellular domain of BCMA(eBCMA), also called decoy receptors, have beenused as a potential BLyS antagonists to block BLyS activities. In order to design novel BLyS antagonistpeptides, computer-aided homologue modeling was used to construct an eBCMA-Fc fusion protein based on thecrystal structures of BCMA and Fc fragmant. To ensure the activity of eBCMA not to be interfered by Fcfusion, the root mean square distance (RMSD) for eBCMA and Fc were calculated to be 0.036 nm and 0.064nm, respectively, based on molecular docking modeling. An eBCMA-Fc fusion gene was constructed andintroduced into E. coli for expression. As expected, the purified 36 kD eBCMA-Fc fusion protein was able tobind BLyS in vitro at a dosage-dependent manner and demonstrated an anti-proliferative activity induced byBLyS in Daudi cells. The results have provided useful information on the evaluation of computer modeling andthe in vitro biological activity for the design of potential BLyS antagonist peptides.

BACKGROUND: Clinically Alzheimer disease (AD) is characterized by obscurity in onset, decline of intelligence, and dysfunctions of behavior and nerve system. The essence of AD is impairment of memory and cognition.OBJECTIVE: To explore the effective mechanism of tiaoxin recipe (TXR)and bushen recipe (BSR) in treating AD.DESIGN: Randomized controlled study.SETTING: Shanghai Institute of Traditional Chinese Medicine for Geriatrics.PARTICIPANTS: Totally 60 AD patients were selected from Shanghai Institute of Traditional Chinese Medicine for Geriatrics from October 1999to January 2000.METHODS: Sixty patients with AD were divided into 3 groups, with 20in each group. The patients in TXR and BSR group took respectively TXR and BSR 10 mL once, 2 times per day. The patients in donepezil (Dp)group took a Dp capsule once a day, h.s (Dp was produced in England Boots Company, 5 mg per capsule). For a course consisted of 12 weeks.The changes of Mini mental state examination (MMSE) score, Activity of Daily Living Scale (ADL) score, neuropsychological testing (NPT) score,including FULD object-memory evaluation (FOM), rapid verbal retrieval (RVR), digit span test (DS) and block design (BD), as well as the overall operational evaluation before and after treatment were analyzed.MAIN OUTCOME MEASURES: Scores of MMSE, ADL, NPT and the overall operational evaluation.RESULTS: Totally 60 patients entered the final analysis. ① MMSE scores in the 3 groups were decreased after treatment as compared with those before treatment (P ＜ 0.01). Total effective rates were 70%, 65% and 75% respectively (P ＞ 0.05). ② ADL scores in the 3 groups were decreased after treatment as compared with those before treatment (P ＜ 0.05or P ＜ 0.01). Total effective rates were 45%, 45% and 40% respectively (P ＞ 0.05). ③ Total effective rates were 70%, 65% and 75% respectively (P ＞ 0.05). ④ Scores of FOM were increased in the TXR and the Dp group after treatment as compared with those before treatment (P ＜ 0.05). RVR scores in the three groups were increased after treatment as compared with tbose before treatment (P ＜ 0.05); DS scores were increased in TXR group and Dp group after treatment as compared with those before treatment (P ＜ 0.05 or P ＜ 0.01); and BD scores in BSR group were increased after treatment as compared with those before treatment (P ＜ 0.05).CONCLUTION: TXR, BSR and Dp can improve cognitive function and daily living ability of AD patients, and TXR and BSR are effective TCM drugs in treating AD.