Objective To investigate the expression of high mobility group A1 (HMGA1) in patients with lung squamous cancer and its clinical significance.Methods The expression of HMGA1 in 76 patients with lung squamous cancer (lung squamous cancer group) and 30 cases of control lung tissue (control group) was detected by SP method of immunohistochemistry and its correlation with clinical-pathological parameter was analyzed statistically.Results The positive expression rate of HMGA1 was 68.4％ (52/76)and 16.7％ (5/30) in lung squamous cancer group and control group respectively,and the difference was significant(P＜ 0.01).HMGA1 expression showed significant correlation with lymph metastasis and degree of differentiation in patients with lung squamous cancer (P ＜ 0.05).Conclusions The progression of lung squamous cancer may correlate with the increase of HMGA1 expression.It's promising for HMGA1 to become a new marker for diagnosis of lung squamous cancer.Clinical detecting of HMGA1 could help with prognosis and treatment-guiding of lung squamous cancer.

With the development of assisted reproductive technology , it brings the gospel to many infertility families , and it makes a new challenge to the moral and legal at the same time .Ministry of Health issued the notice on carrying out the special action to manage assisted reproductive technology .By thinking about the ethical and le-gal issues, this paper tried to explore a new response mode to advance the development of assisted reproductive technology and to ensure social harmony and stability .

With the development of the dialysis technology,the survival time of patients with chronic renal failure is prolonged,while more complications which affect the quality of life or even threat the life of patients are followed.Secondary hyperparathyroidism(SHPT)with a certain incidence and mortality is one of the common complications,and its main characteristic performances ale hyperphosphatemia,hypocalcemia and high level of parathyroid hormone.Most patients Can be cured by the treatment of medicine while surgical treatment is still required by some cases in which the disorder of the parathyroid function can not be well regulated through medicine treatment.The purpose of this paper is to make a review of the recent studies of surgical treatment in patients with SHPT.

Objective To observe the influence of intensive insulin therapy on inflammatory fac-tors and prognosis in severe multiple trauma patients.Methods A total of 53 cases of severe multiple trauma were randomly divided into the treatment group(n =27)and the control group(n =26).Besides basic treatment,patients in the treatment group received additional intensive insulin therapy by micro-pump.The level of blood glucose in the control group was controlled under 11.1 mmol/L.Levels of TNF-α,IL-1β,IL-6,and CRP were tested before and after treatment.Multiple organ dysfunction syndrome,noso-comial infection rate,and mortality rate were also observed.Results The levels of TNF-α,IL-1β,IL-6, and CRP in the treatment group were significantly lower than those of the control group(P <0.05 or P <0.01).The incidence of multiple organ dysfunction syndrome,nosocomial infection,and mortality rate in the treatment group was lower(P <0.05).Conclusion Intensive insulin therapy can effectively decrease the expressions of inflammatory factors in patients with severe multiple trauma,improve the prognosis,re-duce the incidence of nosocomial infection and mortality.

Objective To investigate the risk factors of ischemic stroke in Jiangsu Taixing population.Methods Case-control study was used in this investigation.Cases: all the diagnosed ischemic stroke patients in neurology department of Taixing People 's Hospital from 2011.9 to 2013.6.Controls: all the controls were selected randomly from community residents in Taixing city during the same period of time, according to the age and gender and ratio of 3:2.Univariate analysis and Logistic regression model was applied to analyze risk factors respectively.Results Seven hundred and seventy-seven stroke patients ( case group) and 518 controls ( control group) were brought into the study.It was preliminarily found in univariate analysis that compared with the control group, the rates of smoke, hypertension, diabetes, hyperlipidemia and the waist circumference, waist-to-hip ratio were significantly higher in the case group;while the total bilirubin level was significantly lower ( all P<0.001 ) .Logistic regression showed that smoking (OR=2.089, 95%CI:1.452-3.005), hypertension (OR=3.838, 95%CI:2.516-5.855) were risk factors of stroke in the elder population in Taixing, elevated total bilirubin level ( OR=0.281, 95%CI:0.155-0.508) was protective factor of stroke in the elder population ( all P<0.05 ).Conclusion No smoking, no hypertension history could make it less possible for stroke to occur, and total bilirubin level becomes protective factor of stroke.

Carbamazepine ( CBZ ) serum and salivary concentration and toxic and side reactions were studied in 60 epilepsy patients. The propriate CBZ serum concentration was l7~38?mol/L, salivary concentration was 4~13?mol/L. The toxic and side reactions were correlated with serum level, there were different toxic symptoms when CBZ levels were in therapeutic range. When CBZ serum concen tration was higher than 38~51 ?mol/L, salivary concentration was higher than 14.8~17 ?mol/L, the toxic and side reactions were significant increased in our test patients.

OBJECTIVE:To study the pharmacokinetics of tramadol hydrochloride paracetamol tablets in healthy volunteers.METHODS:10 healthy volunteers were assigned to receive oral single dose of tramadol hydrochloride paracetamol tablets.Plasma concentrations of tramadol hydrochloride and paracetamol were measured by HPLC and the pharmacokinetic parameters were computed by 3p97 software.RESULTS:The concentration-time curves of tramadol hydrochloride and paracetamol all fitted one compartment open model.The pharmacokinetic parameters of tramadol hydrochloride and paracetamol were as follows:t1/2Ka were(0.23?0.16)h and(0.64?0.60)h,t1/2ke were(2.12?0.78)h and(5.48?2.90)h,tmax were(0.90?0.43)h and(1.88?1.12)h,Cmax were(9.37?2.56)?g?mL-1 and(115.27?98.88)ng?mL-1,CL/F(s) were(24.52?7.12)L?h-1 and(0.15?0.08)L?h-1,AUC0～t were(30.38?7.47)?g?h?mL-1 and(598.36?232.14)ng?h?mL-1,AUC0～∞ were(33.02?9.15)?g?h?mL-1 and(800.12?420.92)ng?h?mL-1,MRT0～t were(3.75?1.41)h and(5.46?1.64)h,MRT0～∞ were(5.24?2.92)h and(9.86?6.00)h,respectively.CONCLUSION:This study provided a basis for clinical application of tramadol hydrochloride paracetamol tablets.

OBJECTIVE:To study the relative bioavailability and pharmacokinetics of salbutamol sulfate orally disinte?grating tablets.METHODS:A single dose of8mg salbutamol sulfate orally disintegrating tablets or commercial salbutamol tablets was administered in a randomized crossover design in18volunteers and the plasma concentrations of salbutamol were determined by HPLC.The pharmacokinetic parameters were calculated with3p97pharmacokinetic program and the bioequiv?alency was evaluated.RESULTS:The concentration-time curve of two preparations fitted to two-compartment model.The peak plasma levels(C max )of salbutamol sulfate orally disintegrating tablet and commercial salbutamol sulfate tablet were(17.65?6.48)ng/ml and(16.60?6.21)ng/ml,respectively.The peak time(T max )were(1.92?1.18)h and(2.03?1.17)h and AUC 0～24 were(127.23?32.41)ng/(h?ml)and(131.42?37.73)ng/(h?ml),respectively.The relative bioavailability of salbu_ tamol sulfate orally disintegrating tablet was(99.32?15.58)%.CONCLUSION:The results of two one-side tests suggests that salbutamol sulfate orally disintegrating tablet is bioequivalent to the commercial salbutamol tablet.

OBJECTIVE: To study distribution and pharmacokinetics of gatifloxacin in ocular tissues of rabbit after ocular instillation.METHODS: The drug concentrations in ocular tissues were determined by HPLC after topical ocular instillation in rabbits;the pharmacokinetics parameters were figured out by 3p97 process. RESULTS: Results showed that the peak concentration of gatifloxacin in lacrimal fluid, cornea, aqueous humor, iris-ciliary body, lens and vitreous body 0.125h～1.0h after topical instillation were (1 115.75?151.04)?g/ml,(17.90?1.39)?g/g,(2.26?0.18)?g/ml,(9.12?0.33)?g/g,(1.07?0.13)?g/g and (0.77?0.04)?g/g respectively;t1/2? in various tissues were(2.14?0.33),(2.82?0.54),(3.33?0.43),(3.88?1.21),(3.89?1.43) and (3.14?0.43)h respectively;AUC(0→t) in various tissues were(541.77?69.73)(?g?h)/ml,51.31?3.04)(?g?h)/g,(6.55?0.45)(?g?h)/ml,(51.99?25.66)(?g?h)/g,(4.19?0.41)(?g?h)/g and (3.25?0.13)(?g?h)/g respectively.CONCLUSION:Gatifloxacin has a good permeability and high concentration in various intraocular tissues of rabbit after ocular instillation.