Objective:To investigate the proper method for treating traumatic chyloperitoneum. Methods: A retrospective analysis was performed on clinical data of 4 cases of traumatic chyloperi- toneum admitted to our hospital in recent 26 years. Results: All 4 cases were cured after conserva- tive treatment. Conclusion: Perdominantly integrated therapy including absolute diet,total parrenter- al nutrition(TPN) and abdominal cavity drainage can be the effective method for the treatment of trau- matic chyloperitoneum.

Objective To investigate the biological effects of nonthermal plasma (NTP) combined with X-ray irradiation on human hepatocellular carcinoma in nude mice.Methods By subcutaneous inoculation, tumor models of human hepatocellular carcinoma HepG2 in nude mice were established, and these mice were divided into control group, NTP treatment group, X-ray irradiation group, combined treatment group.The changes of tumor microenvironment were observed using hematoxylin-eosin (HE) staining.The terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) was used to determine the level of neuronal apoptosis in tumor tissues.The expression of metal matrix proteinase-2 (MMP-2) was detected by immunohistochemical assay.Transmission electron microscope was used to observe cell structure changes.Results The final tumor volumes of control group, NTP treatment group, X-ray irradiation group, combined treatment group were (543.96±108.45), (436.54±65.49), (351.66±56.68), (281.97±35.60)mm3, with a statistically significant difference (F=9.63, P=0.01), and the difference between X-ray radiation group and the combination treatment group was significant (P=0.05).HE staining showed that there was a larger area of necrosis in the combined treatment group compared with the other groups.TUNEL showed that the apoptotic indexes were (0.95±0.13)%, (5.82±0.26)%, (7.53±0.43)%, (11.07±0.35)% respectively, with a statistically significant difference (F=547.76, P=0.00), and the difference between X-ray radiation group and the combination treatment group was statistically significant (P=0.00).The immune scores of each group were 12, 9, 9, 2.Electron microscopic observation showed that there were more apoptotic bodies in the combined treatment group than those in the other groups, accompanied by mitochondrial edema.Conclusion NTP and X-ray irradiation therapy in the treatment of human hepatocellular carcinoma in nude mice model has a synergistic effect.Probably, it can be a new type of treatment in curing cancers.

Objective To investigate the expression of aquaporin-1 (AQP1) in human peritoneum and its effect on peritoneal dialysis (PD) patients with the different peritoneal transport characteristics.Methods Peritoneal biopsies were obtained from the PD patients (n =30) at catheter insertion.Western blot and immunohistochemical staining were used to investigate AQP1 expression in peritoneal tissues.After catheter insertion,4 week,peritoneal equilibrium test (PET) was used to decide peritoneal transport characteristics.Results Western blot revealed a major band at 28 kDa band.The expression of AQP1 index was 1.49 ±0.67 in the normal subjects,and 1.61 ±0.76 in the PD patients,and there was no significant difference.Positive deposition was distributed in mesothelial cells,endothelial cells of capillaries,and small veins.The index of AQP1 expression in endothelial cells of capillaries in normal subjects and PD patients were 2.12 ±0.18,and 2.50 ±0.81,respectively,and no significant difference was found.The index of AQP1 expression in mesothelial cells of capillaries in normal subjects and PD patients were 1.95 ± 0.67,and 2.23 ± 1.07,respectively,and no significant difference was found.Among PD patients,the level of AQP1 was significantly different.Conclusions The expression of AQP1 was different in the PD patients with different peritoneal transport characteristics.In the high and high average transport groups,the level of AQP1 was lower; the low transport group was higher.It reveals that AQP1 plays a role in the pathogenesis of transcellular water transport.

Objective To determine the expression of CD38 and HLA -DR on CD +8 T cells in patients with chronic hepatitis B and HBV carriers,and to disscuss the relation between immune activation and disease progression of HBV infection.Methods Thirty -two chronic hepatitis B patients receiving adefovir dipivoxil treatment,31 HBV carriers and 28 normal controls were collected.The counts of CD +4 and CD +8 T cells and the percentage of CD +8 CD +38 and CD +8 HLA -DR +T cells were tested by flow cytometry.HBV DNA and liver function were tested in the central laboratory of our hospital.Results The percentage of CD +8 CD +38 T cells in CHB patients was (58.4 ±12.7)%,and was higher than that in HBV carriers (46.8 ±8.5)% and normal controls (46.8 ±8.5)%,and decreased after adefovir dipivoxil treatment (34.2 ±9.4%)(F =8.27,P =0.000).The percentage of CD +8 CD +38 T cells in HBV carriers was (43.3 ±12.5)%,and was much higher than that in normal controls (9.8 ±5.7)%(F =13.48,P =0.000).The percentage of CD +8 HLA -DR + T cells in CHB patients was higher than that in normal controls,but similar to that in HBV carriers (37.1 ±11.3)%.CD +8 HLA -DR + T cells in CHB patients also decreased after adefovir dipivoxil treatment (P <0.05 ).Conclusion Our study demonstrates that activation of CD +8 T cells is increased in HBV infection but decreased by adefovir dipivoxil treatment.The percentages of CD +8 CD +38 and CD8 +HLA -DR +T are good markers for disease progression of HBV infection.

Objective To analysis the rational use of human albumin in patients with hypoproteinemia in our hospital.Methods The drug evaluation criteria of human albumin were developed and the patients of different gen-dem,ages,departments,clinical diagnoses,human albumin species,administration,doses,course of treatment and pur-poses in our hospital were investigated.Results The using rate of human albumin in patients with hypoproteinemia was 53.1% in our hospital,20% human albumin accounted for the most(89cases),and the consumption of human albumin used in department of respiration was the most in our hospital (70cases).Diagnosis of 14 cases were not anastomosis with indications and 8 cases hadprolonged use and no indicative use and inappropriate medication. Conclusion The irrational use of human albumin are existed in our hospita1.We should develop the drug using standard of human albumin and strictly grasp the indications of drug use to avoid overuse of human albumin.

Patent ductus venous is a kind of the congenital portosystemic shunts (CPSS). In recent years, the related case report increased gradually, but the domestic research is rare. In this article, we review the current diagnosis and treatment of neonatal patent ductus venous based on literature available.

Objective To evaluate the clinical significance of K-ras mutation in plasma cell-free DNA from patients with colorec-tal carcinoma .Methods Plasma cell-free DNA in 212 patients with colorectal carcinoma were extracted and identified by Globin gene .K-ras mutation was amplified by RE-PCR and compared with results of pathological biopsy samples DNA .Results Plasma cell-free DNA were successfully obtained from 212 cases .But no plasma cell-free DNA were found in normal control .Out of 212 ca-ses ,34 cases showed K-ras mutation(16 .00% )in cell-free DNA ,and 33 cases showed positive in pathological biopsy samples DNA (15 .57% ) .It was found two kinds of K-ras mutation on code 12-GGT to GAT ;GGT to GTT .K-ras mutation was not related to tumors′position(χ2 =3 .34 ,P=0 .61);to differentiation degree(χ2 =2 .38 ,P=0 .29);to Dukes staging(χ2 = 2 .84 ,P=0 .38);to lymphoid nodes′metastasis(χ2 =0 .98 ,P=0 .37);to age(χ2 =1 .86 ,P=0 .39) .Conclusion Tumor-derived DNA can be detected in plasma of underlying cancer patients .For colorectal carcinoma patients ,detecting K-ras mutation of plasma cell-free DNA have the same clinical significance as biopsy samples .Moreover ,it′s more convenient and non-invasive .

Forty-six anorexia kids were treated with pinching muscles along the spine, tonifying Pitu (spleen-earth), pressing and kneading Zusanli (ST 36) as well as needling Sifeng points (Ex-UE 10) and got recovery in 20 cases, better in 24 cases and failure in 2 cases with the total effective rate of 95.7% .

Objective:To explore the relationship between plasma endothelin and migraine attack. Methods:The plasma endthelin(ET-1) levels were measured by radioimmunoassay in 54 patients with migraine ( classic: 18, common:36 ) and 20 patientsvith tension headache. Results:The plasma ET-1 level in patients with migraine attack was higher than that of normal con-trols or patients with tension headache (P ＜0.01). During intermission, the ET-1 level of patients with classic migraine washigher than that of patients with common migraine (P ＜0.01),and the ET-1 level in male patients was higher than in fe-male ones. Conclusion: The data suggest that there are abnormalities of vascular endothelial cell system in patients with mi-graine. ET-1 may be one of factors causing migraine attack.

Objective To study the influence of splenectomy on the surgical efficacy in the patients with gastric cancer in the up-per and middle portion and entire stomach .Methods 86 patients with gastric cancer in the upper and middle portion and entire stomach receiving the gastric cancer D 2 radical operation in the two hospitals from April 2003 to January 2008 were selected and di-vided into the observation group(47 cases) and the control group(39 cases) .The observation group accepted the modified lymph-adenectomy with spleen-preserving ,while the control group accepted the D2 total radical gastrectomy with splenectomy .The long-term effects were compared between the two groups .Results The total hospitalization time and the occurrence rate of postoperative complications were(20 .62 ± 7 .93)d and 10 .64% in the observation group ,which were less than (27 .51 ± 12 .44)d and 30 .77% in the control group ,and the differences between the two groups showed the statistical significance (t=3 .128 ,χ2 =5 .446 ,P=0 .000) . The 5-year survival rate in different TNM stages had no statistical difference between the two groups (P>0 .05) .Conclusion For the patients with gastric cancer in the upper and middle portion and entire stomach without spleen or splenogastric ligament metas-tasis ,the modified spleen-preserving lymphadenectomy could make the patients to get the same prognosis as the gastrectomy com-bined with splenectomy ,which reduces the occurrence rate of complications and is worth promoting clinically.