Objective To develop developer with fast determining stopped spot of electrolyte electrode in wartime.Methods According to certain proportion configuration,the developer was prepared by using relative reagent and distilled water.Through injector to take 1ml developer and curve syringe needle,then straight insertion the type needle of electrolysis meter and pour into the developer to fill suction tube.Results The reagent can be seen various electrodes change and fast display the electrode breakdown anywhere.Conclusion The color developing reagent is low-cost,efficient and convenient to use.

10.3969/j.issn.1000-3606.2013.06.001

Congenital cataract is a common eye disease which leads to children low vision. Surgery is the main treatment. Because of children's preoperative evaluation, operation, postoperative management is more difficult than adult, the outcome of surgery is not so well. Therefore, we review the relevant congenital cataract surgery researches in recent years in order to discuss the problems and further developments in this area.

The afferent connections of the preoptic region in the rat were studied with retrograde horseradish peroxidase (HRP) method.The mico-injection syringe needle or microelectrophoretic pipette was inserted vertically into the brain. In some cases, the injection was made with the needle inclined and directed contralaterally or in inclined direction through the cerebellum.The results were as follows:Following HRP injection into the medial preoptic area, labeled neurons were found in periventricular hypothalamic gray, paraventricular nucleus, anterior hypothalamic nucleus, supraoptic nucleus, ventromedial hypothalamic nucleus, posterior hypothalamic nucleus and locus coeruleus in all cases. In some cases, labeled cells were also observed within bed nucleus of stria terminalis, dorsal raphe nucleus. In addition, in a few cases, a small number of labeled cells could be found in lateral septal nucleus, medial septal nucleus, periaqueductal gray matter and interpeduncular nucleus.After injection into the region between the medial and lateral preoptic area, besides the above mentioned labeled nuclei, there were still a number of labeled cells found in paratenial nucleus, nucleus reuniens and lateral hypothalamic nucleus, in some cases also found in the supramammillar nucleus, and in one case even in the cortical and medial amygdalar nuclei.Following the injection of HRP into the anterior hypothalamic nucleus, retrograde labeling of neurons was observed within the lateral septal nucleus, preoptic periventricular nucleus, medial preoptic nucleus, supraoptic nucleus, periventricular hypothalamic nucleus, paraventricular nucleus, ventromedial hypothalamic nucleus, periaqueductal gray matter and locus coeruleus in all cases. Among them, supraoptic and paraventricular nuclei were labeled bilaterally in some cases.

Fifteen liposoluble compouuds were isolated and identified from the rhizomes of Drynaria fortunei (Kunze) J.Sm.. They are: diploptene(Ⅰ); hop-21-ene(Ⅱ); diplopterol(Ⅲ); fern9(11) -ene(Ⅳ); cyclolaudenol(Ⅴ); cyclomargenol(Ⅵ); cyclolaudenone (Ⅶ); n-dotriacontanic acid(Ⅷ); ?-sitosterol(Ⅸ); 25-en-cycloartenol(Ⅹ); 25-en-cycloartenone(Ⅺ); 24-en-cycloartenol(Ⅻ); 24-en-cycloartenone; 5-stigmasten-3-ol; 5-stigmasten-3-one Apart from Ⅰ,Ⅳ and Ⅸ, the rest had not been found in this fern since.

AIM:The complications following liver transplantation consist of the liver damage and the function failure of other organs,among which lung is the most prone and the earliest affected lesion.By establishing an autologous orthotopic liver transplantation(OLT) model in rats,this study was to investigate the acute lung injury after liver transplantation and the role of alkaline phosphatase(AP) in protection of the injury.METHODS:①The experiments were performed in the Test Animal Center and Digestion Laboratory,Nanfang Hospital,Southern Medical University from May to October in 2007.All the proposals were in accordance with the ethical standard of animals.② Twenty-four SD rats were randomly divided into three groups:sham-operation control group,autologous OLT group and AP group,each containing 8 animals.Autologous OLT models were induced in OLT group and AP group,while control group,the abdomen was only opened and then closed besides the liver lobes were isolated.Lingual vein injection of AP was given in AP group 5 minutes before the blood supply of the liver restored.③The concentration of myeloperoxidase(MPO),malondialdehyde(MDA) and surfactant protein A(SP-A) in lung tissue were respectively examined in each group,lung dry-to-wet weight ratio was also measured.Furthermore,hepatic and lung pathological sections were observed.RESULTS:All the 24 rats were involved in the result analysis.①Compared to control group,the concentration of MPO and MDA in lung tissue were significantly increased in OLT group and AP group(P

Objective To investigate whether the early use of tetramethylpyrazine(TMP) could improve the oxygenation ,reduce the rate of pulmonary lesion ,increase the survival rate and shorten the hospital days in the patients with severe acute pancreatitis (SAP) .Methods A total of 42 patients with SAP were randomly divided into the control (C) group(21 cases ,receiving the conven-tional treatment combined with the respiratory support treatment ) .The TMP group(21 cases ,receing the conventional treatment , respiratory support and TMP treatment ) .Results In the C group ,6 cases were intubated(28 .57% ) ,9 cases were acute respiratory distress syndrome(ARDS)(42 .86% ) ,the survival rate was 80 .95% ,the average hospitalization was (21 .6 ± 7 .3)d;in the TMP group ,3 cases were intubated(14 .28% ) ,6 cases were ARDS(28 .57% ) ,the survival rate was 85 .71% ,the average hospitalization was(17 .9 ± 6 .4)d .The survival rate had no statistical difference between the two groups (P>0 .05) .In addition ,the TMP group was superior to the C group in the average hospital days .Conclusion Eerly TMP interrention significantly reduced the rate of endo-tracheal intubation and the occurrence rate of ARDS ,shorten the hospital days ,but without increasing the survival rate .

The application of military medical equipment highly influences the effect of medical support.According to the investigation in the affiliated basic units,this paper puts forward improvement measures for the existing problems in the application of military medical equipment.

Strategic information reservation of medicinal materials is complementary to the strategic reservation of medicinal materials itself.This article discusses the advantages and disadvantages of the reservation of physical medicinal goods and mobilization of medical resources for national defense.The construction mode and operational characteristics of the information database for the strategic reservation of medicinal materials have also been discussed as well as the great significance of its widely application to the information reservation of medicinal materials.

Objective To explore the effect of baicalin on tumour necrosis factor-?(TNF-?) and soluble inter-cellular adhesion molecule-1(sICAM-1) of immunosuppressed rats infected with Pneumocystis carinii.Methods Forty-nine SD rats were randomly divided into 7 groups:normal control(A), immunosuppressed control(B), SMZ/TMP control(C), baicalin prevention(D), low dose(E), moderate dose(F) and high dose(G).Rats of group D received six injections at three-day intervals with 3.5 mg dexamethasone sodium phosphate for 3 weeks, while groups B, C, E, F and G received same immunosuppressor but for 6 weeks.Rats in group D were given 100 mg/kg baicalin daily for 5 weeks, and groups C, E, F and G were given 250 mg/kg SMZ+50 mg/kg TMP, low dose 100 mg/(kg?d), moderate dose 200 mg/(kg?d), and high dose 400 mg/(kg?d) baicalin daily for 2 weeks, respectively.At the end of 8th week after immunosppression, the contents of TNF-? and sICAM-1 in peripheral blood were detected by radioimmunoassay(RIA) and ELISA, respectively.The pathological change of lung was observed by lung imprint smear with gormori methenamine silver(GMS) staining and lung section with hematoxylin-eosin(HE) staining.Results The content of TNF-? in group D(2.14?0.14) ng/ml, group E(2.57?0.15) ng/ml, group F(1.46?0.14) ng/ml, group G(1.12?0.13) ng/ml andgroup C(1.59?0.14) ng/ml were higher than that of group A(0.70?0.21)ng/ml(P