Objective To explore the self efficacy of newly-contracted nurses in a tumor hospital in Guangzhou and to find out the related factors. Methods By convenience, cluster sampling method, 333 newly-contracted nurses from a tumor hospital in Guangzhou were recruited in the study. The general self-efficacy scale (GSES) and a self-designed demographic questionnaire were used to look into their self-efficacy and explore the related factors. Results The average score of self efficacy was (2.67 ± 0.41). The level of self-efficacy was related to education and the type of hospital for internship. Nurse with bachalor degree and graduated from comprehensive hospital had higher self-efficacy. Conclusions The self efficacy of the newly contracted nurses is intermediate. The education level and the type of hospital for internship both influence the level of self efficacy. The special hospitals should strengthen the teaching and training of students and lay stress on developing their capacity to prepare them for the job.

0.05).Conclusion Cyclin B1 levels in liver cancer tissues were stronger than in adjacent cancer tissues and normal liver tissues.

Objective To investigate the p33~ ING1b mRNA level and the relationship to clinical pathological data in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) method was employed to detect the mRNA level of p33~ ING1b in liver cancer tissues and adjacent tissues of cancer.Results p33~ ING1b mRNA was positive expression in 63.6% (35/55) cancer tissues and 70.9% (39/55) adjacent tissues of cancer.The expression level of p33~ ING1b mRNA in cancer tissues (0.410?0.175) was significantly lower than that in adjacent tissues of cancer (0.529?0.203). The level of p33~ ING1b was significantly associated with pathological grades, lymph node metastasis and envelope infiltration, but not associated with sex, age, tumor size, tumor thrombus or liver cirrhosis.Conclusion The decrease of p33~ ING1b may play an important role in the tumorigenesis and development of PHC, and may be an adjuvant parameter in evaluating tumor differentiation, invasion and prognosis.

Objective To approach the cause and treatment of complications during and after catheter closure of ventricular septal defect(VSD).Methods Catheter closure of ventricular septal defect was attempted in 415 patients.Among them,74 patients with complications were retrospectively analyzed.Results The overall complication rate was 17.83%(74/415).The complications during procedure were seen in 13.98%(58/415) which included arrhythmias in 11 patient,aortic regurgitation in 15 patients,residual shunt in 26 patients,displacement of closure device in 1 patients,cardiac temponade in 1 patient,tricuspid valve injury in 1 patient,mitral valve injury in 1 patient,iatrogenic VSD in 1 patient and respiratory complication in 1 patient.The complications after procedure were noted in 4.19% of the patients(16/382),including arrhythmias in 8 patients,LV enlargement in 2 patients,thrombus fomation in femoral artery in 2 patients,Ⅲ?AVB in 2 patients whom required temporary and permanent pacemaker implantation,respectively,headache in 4 patients,haemolysis due to residual shunting in 2 patients,tricuspid valve injury in 1 patient and puncture site bleeding in 1 patient.There was no death recorded in our study.Conclusion The incidence of complications during and after catheter closure of ventricular septal defect was relatively low but careful long term follow-up is necessary for the monitor of delayed complications.

0 05) between the two groups in the patient′s age, pregnancy age, parities, the cavity volume of uterine, hemoglobin and blood platelet count The bleeding volume was (51 6?17 2) ml for the treated group and (63 3?17 1) ml for the control group ( P 2 months It could decrease the volume of bleeding during the operation and benefit for patients recovering

Objective To approach the cause and treatment of complications during and immediately after percutaneous balloon mitral valvuloplasty. Methods One thousand three hundred and eleven patients with mitral stenosis were treated by percutaneous transseptal balloon mitral valvuloplasty. Among them, 42 patients with complications were retrospectively analyzed. Results The overall complications rate was 3.2% (42/1 311) including atrial fibrillation 0.8%(10/1 311), acute pericardial tamponade 0.31%(4/1 311), severe mitral insufficiency 0.46% (6/1 311), femoral arterial venous fistula 0.69% (9/1 311), acute pulmonary edema and iatrogenic atrial septal defect 0.23%(3/1 311), respectively. Coronary air embolism, arterial thrombosis and transient cerebrovascular accident was 0.15%(2/1 311) for every other one. Balloon rupture was 0.08%(1/1 311).Conclusions The complications of percutaneous balloon mitral valvuloplasty rarely occur. It is a safe and efficient nonsurgical method for treating rheumatic mitral stenosis.

Objective To investigate the expression level and clinical significance of cyclinB1 in primary hepatocellular carcinoma(PHC).Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the mRNA level of cyclinB1 in liver cancer tissues and adjacent tissues of cancer.Results CyclinB1 mRNA was positive expression in 90%(36/40)cancer tissues and 85%(34/40)adjacent tissues of cancer.The expression level of cyclinB1 in cancer tissues (0.531?0.015)was significantly higher than that in adjacent tissues of cancer(0.263?0.023).The level of cyclinB1 was significantly associated with pathological grades and lymph node metastasis, but not associated with sex、age、tumor size or tumor thrombus.Conclusion CyclinB1 may play an important role in the tumorigenesis of PHC and may be a potential adjuvant parameter in evaluating pathological grades and lymph node metastasis.

Objective To study the cytogenetic changes in HPV-immortalized cells(SHEE)for further investigating the esophageal carcinogenesis. Methods Giemsa staining was used to show the modal number of chromosomes.G-banding was done to observe the chromosomal aberrations.Fluorescence in situ hybridization(FISH)of the interphase nuclei with the centromere probes of chromosome 1,7 and 8 were performed to determine the numerical abnormalities. Results The modal numbers at passage 10 and 20 were 58-63 and 57-64, respectively.Passage 61 had a bimodal distribution 58-61 and 63-65.The structural aberrations could almost be found in all of karyotypes.The following structural aberrations were identified:1.del(1)(q12);2.del(1)(p32);3.der(4),t(4;?)(q31;?);4.der(5),t(5:?)(q31;?).FISH results showed that trisomy 1 and tetrasomy 7 markedly increased with the increase of passages and disomy 8 kept in faint change in all passages.Conclusion The modal number moved right slowly in the graph of the modal distribution and the increases of chromosomal numbers were in imbalance with increases of passages of SHEE.The structural aberrations of chromosome were found in most of karyotypes.Our results suggest that the cell immortalization is a multistep process involved in the multiple chromosomes.;

Objective To observe the recent clinical effects and toxicity in advanced gastric carcinoma by neo-adjuvant chemo-therapy FOLFOX and XELOX .Methods A total of 81 patients with Ⅱ ~ Ⅲ level of gastric cancer underwent neo-adjuvant chemo-therapy before curative resection were collected ,and divided into two groups ,as XELOX group(n=26) and FOLFOX group(n=25) .30 patients who just underwent surgical treatment did not received neo-adjuvant chemotherapy were taken into control group(n=30) .The curative resection rate and complications were compared in three groups .Results There were no obvious differences be-tween XELOX group and FOLFOX group in recent clinical effect (P>0 .05) ,but FOLFOX group had higher toxic reaction rate (P<0 .05) .The curative resection rates of XELOX group and FOLFOX group were higher than that of control group (P<0 .05) . The complication rates in three groups showed no significant difference (P>0 .05) .Conclusion Neo-adjuvant chemotherapy could increase the curative resection rate of advanced gastric cancer ,which does not increase the complications ,and the program XELOX had less toxic reaction .

Objective To investigate the clinical significance of detecting mammaglobin in sentinel lymph nodes(SLN) of breast cancer. Methods In 32 breast cancer patients, methylene blue was injected around parechyma of breast cancer to locate the SLNs, and nested RT PCR(reverse transcriptase polgmecane chain reaction) was used to examine the expression of mammaglobin mRNA. Results The SLN was successfully identified in 30 of the 32 cases(93.8%).The micrometastases detection between group SLNs and non SLNs had significant statistical difference(P