Objective: To construct a prediction model for preeclampsia (PE) risk in twin-pregnant women, so as to provide the basis for early screening and prevention of PE. Methods: A total of 467 twin-pregnant women who underwent prenatal examination and delivered at Huzhou Maternal and Child Health Hospital were selected. Sixty cases with preeclampsia (PE) were included in the case group, and 60 women without PE were included in the control group. General information, blood biochemical indicators and uterine artery resistance index (UtA-RI) were collected. A logistic regression model was used to screen predictive factors and establish a nomogram. The Bootstrap method was performed for the internal validation; the receiver operating characteristic (ROC) curve, calibration curve and decision curve analysis were employed to evaluate the discrimination, calibration and clinical utility of the nomogram, respectively. Results: In the case group, there were 47 individuals (78.33%) aged younger than 35 years, 21 individuals (35.00%) with pre-pregnancy body mass index (BMI) of 25 kg/m2 and above, and 33 individuals (55.00%) with in vitro fertilization. In the control group, there were 57 individuals (95.00%) aged younger than 35 years, 8 individuals (13.33%) with pre-pregnancy BMI of 25 kg/m2 and above, and 39 individuals (65.00%) with natural pregnancy. Multivariable logistic regression analysis identified age, pre-pregnancy BMI, method of conception, placental growth factor (PLGF) and UtA-RI as risk prediction factors for PE in twin-pregnant women. The established nomogram had an area under the ROC curve of 0.827 (95%CI: 0.755-0.899), a sensitivity of 0.767, a specificity of 0.733, a good discrimination and calibration, and a relatively high clinical net benefit. Conclusion The nomogram established by age, pre-pregnancy BMI, method of conception, PLGF and UtA-RI has a good predictive value for the risk of PE in twin-pregnant women.

Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Ba-wei capsule .Methods HPLC method was adopted with C18 column(250 mm × 4.6 mm ,5 μm) ,Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode . The detection wavelength was 240 nm and the flow rate was 1.0 ml/min .Results Salvianolic acid B reference substance was excellently linear in the range of 15.026~100.17 μg/ml ,the average recovery was 96.6% (n=6)and RSD was 1.8% (n=6) .Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml ,the average recovery was 97.0% (n=6)and RSD was 1.7% (n=6) .Polydatin reference sub-stance was excellently linear in the range of 8.235-54.90 μg/ml ,the average recovery was 97.2% (n=6)and RSD was 1.8%(n=6) .Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml ,the average recovery was 98.1% (n=6)and RSD was 1.7% (n=6) .Conclusion The method was easy ,accurate ,reproducible ,specific and without in-terference in negative control ,which could be used for quality control of Gan Bawei capsule .

In order to construct the recombinant retrovirus vector of human ngn3 gene and its packaging cell line, we successfully amplified the open reading frame (ORF) of ngn3 gene from human fetal pancreatic tissue by RT-PCR. The PCR products of human ngn3 gene was subcloned into pMD18-T vectors and sequenced. Results showed that its sequence was fully consistent with the ngn3 gene published in GenBank(GenBank Accession No. BC126468). The correct fragment was digested by EcoR I and Hpa I from recombinant pMD18-T vector and inserted into the same restriction enzyme sites of retroviral vector pMSCV-neo. We got recombinant retrovirus vector pMSCV-ngn3, which was identified by double restriction enzyme digestion and then transfected into PT67 cells by lipofectamine 2000. We established the PT67-ngn3 packaging cell line by G418 selection, which was detected by RT-PCR and immunohistochemistry staining. The detection results showed that the Ngn3 expressed at the mRNA and protein level in the packaging cell line. RT-PCR detection and electronic microscope analysis showed that the recombinant retroviral vector pMSCV-ngn3 was packaged into infectious virus particles and released into the supernatant of the cells. These results demonstrated that a PT67-ngn3 packaging cell line was successfully established, and this could facilitate the study of differentiation of the human fetal pancreatic progenitor cells into insulin-producing cells by using the ngn3 gene.
Basic Helix-Loop-Helix Transcription Factors ; biosynthesis ; genetics ; Cell Differentiation ; drug effects ; Cell Line ; Cloning, Molecular ; Fetus ; Genetic Vectors ; genetics ; Humans ; Insulin-Secreting Cells ; cytology ; Molecular Sequence Data ; Nerve Tissue Proteins ; biosynthesis ; genetics ; Open Reading Frames ; genetics ; Pancreas ; cytology ; RNA, Messenger ; biosynthesis ; genetics ; Recombinant Proteins ; biosynthesis ; genetics ; pharmacology ; Retroviridae ; genetics ; metabolism ; Stem Cells ; cytology ; Transfection