From July 1991 to June 2002,80 patients undergoing 19 mm aorta mechanical prosthetic valve replacement and 80 patients undergoing ≥ 21-mm valve replacement were enrolled. There was a significant difference in left ventricular wall thickness,mass index,and pressure gradients in 19-mm valve replacement (P 0.05). The difference of left ventricular wall thickness,mass index,and pressure gradients was lower in group with effective orifice area index (EOAI) 0.75 cm2/m2 (P 0.05). Patients with 19-mm prosthetic aortic valves can experience a satisfactory improvement and intermediate-term survival. It is important for weight-control after replacement.

0.05).The proportion of TR degree improved in patients of two groups in a short-term.However,the difference of TR in long-term between two groups was statistically significant(P

Objective To compare the phage based splitting assay and BACTEC 460 in the rapid detection of Mycobacterium tuberculosis ( M. tuberculosis ). Methods 30 clinical isolates of M. tuberculosis, 10 strains of non M. tuberculosis, 7 strains of non mycobacterium and 60 sputum specimens of pulmonary tuberculosis patients were detected with phage based splitting assay and BACTEC 460 system. Results All the strains of M. tuberculosis clinical isolates detected with phage based splitting assay were positive, while all of the non M. tuberculosis and non mycobacterium strains were negative. 41 sputum specimens with BACTEC 460 culture positive and 19 sputum specimens with BACTEC 460 negative were detected with phage based splitting assay, the number of positive specimens was 34 (82.9%) and 5 (26.3%) respectively.Conclusions The phage based splitting assay can detect the M. tuberculosis easily and quickly in two days with high sensitivity and specificity.

Objective To develop new method for rapid detection of mutations in rpoB gene related with resistance to rifampin of M. tuberculosis . Methods According to the sequence of wild type M. tuberculosis, five oligonucleotide probes covering the 69 bp hyper variable region of rpoB gene were designed and immobilized on nylon membrane strips. Thereafter, the target rpoB gene fragment was obtained by PCR using biotin labeled primers and thereafter the PCR product was denatured and hybridized with probes on membrane. The results of reverse dot blot hybridization were compared with the results of drug sensitivity test and sequencing. Results PCR products from 36 RFP resistant and 22 RFP susceptible isolates were detected by the assay of reverse dot blot hybridization, showing that the susceptibility and the specificity rate were 88.9% and 86.4% respectively,and the coincidence with biochemical method and sequencing is 87.9% and 89.7% respectively. Conclusions Reverse dot blot hybridization is a rapid and sensitive method to detect the rpoB gene mutations,which may be used in early detection of the resistance of M. tuberculosis to rifampin.

Objective:This study assesses the results of the clover technique for the treatment of tricuspid regurgitation(TR) due to severe prolapse or tethering.Methods:From March 2016 to November 2018, 28 patients with severe TR due to prolapsing or tethered or adhesion leaflets underwent clover technique. Annuloplasty was associated in 27 patients(97%). The aetiology of TR was rheumatic in 19 cases(68%), subacute bacterial endocarditis in 4(14%), degenerative in 3(11%) and ischemia of right ventricular(the peacing leads compress the septum leaflet in 1 case) in 2(7%). The main mechanism of TR was prolapse/flail of one leaflet in 7 patients(23%), of two leaflets in 13 patients(46%) and of all three leaflets in 6 patients(21%). The remaining 2 patients(7%) presented with severe leaflets’tethering.Results:None deaths occurred during hospitalisation and one patient dischargedvoluntary 12 days after surgery. Follow-up of the 27 hospital survivors was 100% complete[mean length(1.2±0.8)years, range 0.25-1.70 years]. At the last echocardiogram, no or mild TR was detected in 25 patients(88.7%), moderate(2+ /4+ ) in two(9.6%) and severe(4+ /4+ ) in one patient(3.6%). Mean tricuspid valve area and gradient were(4.3±0.6 )cm 2 and(2.8±1.4)mmHg(1 mmHg=0.133 kPa). There was no obvious valve stenosis in all cases. In all patients, echocardiography was performed and no signs of tricuspid stenosis were detected. At the multivariable analysis, the degree of TR at hospital discharge was identified as the only predictor of TR 2+ at follow-up. Conclusion:Clover procedure is simple and safe in the surgical management of various causes of TR besides severe tethering and calcification, it is an effective supplementary measure for annuloplasty.