This paper summarizes the main methods of civil-military integration,academy of military medical sciences,analyzes the main problems restricting the development of military and civilian integration depth,puts forward to strengthen military medicine civil-military integration of science and technology innovation and development measures.

This paper introduced the feasibility of flexible management from medical scientific re-search institutions, embarks on the aspects of construction contents such as value identity system, fuzzy eval-uation system, sharing mechanism of scientific management aims and information involving in implementing management. And the paper combined with the experience of the innovation projects in Chinese Academy of Sciences and our institution, and preliminarily expounds the construction connotations and keys of the "flexi-ble management system" in medical scientific research units to provide ideas for enriching the management thoughts of medical scientific research.

Drugs for military special needs are important strategic materials for supporting military operational needs and maintaining national security,and are highly valued by Armed Forces in each country in the world.The U.S.military has accumulated much experience through the long-term management of drugs for military special needs.Here,The U.S.military and civilian integration management of drugs for military special needs between the U.S.DOD and FDA was analyzed in terms of research,purchase,use and storage,the secrets of success were summarized,and problems were identified.Finally,recommendations on military and civilian integration management of drugs for military special needs in China were proposed.

Objective To cvaluate the efficacy and safety of HabibTM VesOpen intravascular radiofrequency catheter percutaneous radiofrequency ablation combined with TACE in the treatment of portal vein tumor thrombus of primary hepatocellular carcinoma.Methods Thirty-nine patients with primary hepatocellular carcinoma complicated with portal vein thrombosis were treated with HabibTM VesOpen intracavitary catheter ablation combined with TACE.The postoperative success rate,complication,blood index,α-fetoprotein (AFP),portal vein patency and tumor thrombus imaging changes were assessed.Results All of 39 patients underwent surgery successfully without occurrence of perforation,infection,hepatapostema,intra-abdominal hemorrhage nor other complications associated with surgery.Blood routine examinations (WBC,RBC,PLT) after 2 weeks and 4 weeks of surgery had no statistical difference compared with those of preoperation.Liver function indexes including ALT,AST,ALB had statistical difference before and after the surgery except for TBIL.AFP had statistical difference before and after surgery.Doppler ultrasonography after 4 weeks showed blood flow in original blocked portal vein.Enhanced CT or MR examinations after 8 weeks on the abdomen,suggested that tumors were shrinked or disappeared.Conclusion Radiofrequency catheter ablation with intracavitary radiofrequency catheter of HabibTM VesOpen is an effective method in the treatment of portal vein tumor thrombus in primary hepatocellular carcinoma.

Objective To clone the full-length CDS sequence of LOC 401296 , an undefined gene that we found recently, and to obtain both its expression in eukaryotic cells or E.coli and antiserum to LOC401296 protein.Methods The full-length CDS sequence of LOC401296 was amplified by polymerase chain reaction (PCR).Then we established the expression vectors pET28B-LOC401296 and pCMV-Myc-LOC401296 by cloning the full-length CDS sequence into vector PET28B and vector pCMV-Myc respectively.Isopropyl Thiogalactoside (IPTG) was used to induce LOC401296 expression in E.coli.Furthermore,the protein purified and refolded was used to immunize BALB /c mice.The titer of the antiserum collected from immunized mice was identified by ELISA assay and Western blot .Results We cloned the full-length CDS sequence of LOC401296 was successfully .Protein LOC401296 was expressed as was expected and the mouse anti-human LOC401296 antiserum was obtained .The antiserum titer reaching 1∶64 000 was identified by ELISA .Besides, Western blot analysis showed that the antiserum could be used to detect protein LOC 401296 .Conclusion The full-length sequence of LOC401296 is obtained and the mouse anti-human LOC401296 antiserum becomes available .This study can contribut to further research on the undefined gene LOC 401296 .

Radiation biodosimetry is an important part of radiation medicine. Some new discoveries and progresses have been made in radiation biodosimetry studies in recent years. Cytogenetic method represented by chromosome aberration analysis as the golden standard of radiation biodosimeter is being transformed to automate analysis, and a number of international, regional and national laboratory networks of radiation biodosimetry are being established. As a widely acceptable molecular marker of DNA damage,γ-H2AX has made rapid progress in radiation dose estimation. Based on the expressions of protein and genes, further advancements have been made in the studies of metabolites and miRNAs. At the same time, with the development of proteomics technology, there are some breakthroughs in the study of using molecular expression profiling to evaluate radiation dose. The research progresses of radiation biodosimetry is reviewed in this paper.

With the development of civil military integration,military scientific research institu tes are facing the challenge of constructing a new mode of translating scientific and technological achievements into practice and enhancing translational efficiency.This paper began with the evolution of translation mode in military institutes and discussed the flaws and insufficiency of current mode,then a triple helix translation mode,which encompass government,industry and research,was introduced and fully explained for future reference.

OBJECTIVETo evaluate the prognostic value of the lymph node ratio(LNR) staging system for rectal cancer following 30 Gy/10 f preoperative radiotherapy.

METHODSClinical data of 282 patients with mid-lower locally advanced rectal cancer who received preoperative radiotherapy and curative surgery in the Peking University Cancer Hospital from August 2003 to August 2009 were retrospectively reviewed. The radiotherapy regimen was recommended by CACA. Total dose of 30 Gy was divided into 10 fragments (30 Gy/10 f), the biologically equivalent dose (BED) was 36 Gy, and 3D conformal radiotherapy(3D-CRT) was used. Surgery was performed 2-4 weeks after radiation. The prognostic effect of the lymph node ratio(LNR) staging system in addition to the 7th AJCC staging system were retrospectively analyzed and compared in stage III( cases with positive lymph node(s). Patients were divided into four groups by LNR quantiles: LNR1(<0.070), LNR2(0.070-0.142), LNR3(0.143-0.307) and LNR4(>0.307).

RESULTSA total of 108 eligible cases were included in the study. The 3-year disease-free survival (3-yr DFS) was 61.1%. On univariate analysis, circumferential resection margin involvement(P=0.034), tumor differentiation (P=0.002), N stage (P=0.001), TNM stage(P=0.000) and LNR(P=0.003) were significantly associated with 3-yr DFS. On multivariate analysis, abdominoperineal resection (P=0.006, HR=2.611, 95%CI:1.323-5.153), G3-4 differentiation (P=0.015, HR=2.208, 95%CI:1.163-4.192), ypN2a/N2b stage(as covariate: P=0.024, HR=2.568, 95%CI:1.135-5.810; P=0.001, HR=3.759, 95%CI:1.776-7.958) were independent risk factors for decreased 3-yr DFS. Other factors including LNR were excluded in Cox regression model. The 3-yr DFS was statistically different among subcategories of ypN stage. There was no statistical difference of 3-yr DFS in pair-wise comparison of LNR1 and LNR2, LNR2 and LNR3 and LNR3 and LNR4. Additional use of LNR over AJCC staging system did not improve the prediction of prognosis for III(B/C stages and for each stratum of ypN stages, despite the prognostic separation by LNR in III(A stage.

CONCLUSIONThe LNR staging system in addition to the 7th AJCC staging system does not provide further detailed stratification of the prognosis for stage III( rectal cancer following 30 Gy/10 f preoperative radiotherapy. Lymph node ratio is premature as a prognostic factor in clinical practice.

Disease-Free Survival ; Humans ; Lymphatic Metastasis ; Multivariate Analysis ; Neoplasm Staging ; Prognosis ; Rectal Neoplasms ; pathology ; radiotherapy ; surgery ; Retrospective Studies

Objective:To study the genetic changes and biological potential of proliferative nodule in congenital melanocytic nevus.Methods:Whole-exome sequencing was carried out using the technique of next-generation sequencing (NGS) in order to detect the genomic alterations of two cases of proliferative nodules (PN) in congenital melanocytic nevi (CMN). Twelve cases of CMN and ten cases of malignant melanoma were used as benign and malignant controls, respectively. Mutated genes that possessed statistically significant difference between benign and malignant controls were listed, according to what benign and malignant statuses were classified and clustered. The heatmaps of clustering analyses were depicted using heatmap package. Fluorescence in situ hybridization (FISH) was also used to validate the above results.Results:Eighty-six common somatic gene mutations were detected in two samples of PN. Compared with CMN, PN had 52 more mutated genes. Furthermore, 22 of these 52 mutated genes were also detected in malignant melanoma samples. Two cases of PN fell between benign CMN and malignant melanoma in germline mutation clustering. Both cases of PN were positive in the FISH tests.Conclusions:The genetic changes of PN partially overlap with those of CMN and malignant melanoma. Therefore, although most of the PN manifest as a benign lesion clinically, it may have certain malignant potential at the genetic level, and warrant long-term monitoring and follow-up.

Objective To investigate the value of colonoscopic assessment in"watch and wait"strategy for mid?lower rectal cancer after neoadjuvant chemoradiotherapy (nCRT). Methods A single?center retrospective case series study was performed. Database of mid?lower rectal cancer patients at Department of Gastrointestinal Oncology, Peking University Cancer Hospital&Institute from March 2011 to June 2017 was retrieved. Inclusion criteria: (1) nCRT was completed (50.6 Gy/22 f, plus oral capecitabine); (2) radical surgery was performed within 12 weeks after nCRT treatment; (3) clinical response to nCRT was determined as clinical complete response (cCR) or near?cCR. Patients who did not undergo colonoscopy and MRI in our center during initial assessment and follow?up, or whose colonoscopy data were unable to re?evaluated, were excluded. Initial evaluation of nCRT response was carried out between 6 and 16 weeks after nCRT. The results of endoscopy (eCR, near?eCR and non?eCR) and MRI (mCR, near?mCR and non?mCR) were compared to local lesion relapse during follow?up. The consistency of the results of colonoscopy and MRI was evaluated by Kappa test (Kappa value of 0.21 to 0.40 indicates general consistency, 0.41 to 0.60 moderate consistency, and 0.61 to 0.80 high consistency). The non?regrowth disease?free survival (NR?DFS) curves of the eCR group and the near?eCR group were plotted by Kaplan?Meier method and compared by log?rank test. Clinical significance of colonoscopy examination in the following"watch and wait"strategy during follow?up period was analyzed. Results A total of 32 patients were enrolled in the study, including 21 (65.6%) males and 11 (34.4%) females with a median age of 57 years old. The differentiated type of rectal cancer included 1 (3.1%) case of well?differentiated, 26 (81.2%) of moderately differentiated and 5 (15.6%) of poorly differentiated. Clinical stage of the patients included 9 (28.1%) cases of T2?3N0 and 23 (71.9%) of T2?3N+. Median follow?up period was 48 (18 to 80) months. The local regrowth rate was 34.4% (11/32) and median interval of local regrowth was 10.0 (4 to 37) months. Initial colonoscopy evaluation was carried out at a median time of 9 (5 to 19) weeks after nCRT was completed. According to endoscopic findings, patients were divided into 3 groups, including 15 cases in eCR group, 15 cases in near?eCR group and 2 cases in non?eCR group. According to the appearance of MRI, patients were divided into 3 groups, including 8 cases in mCR group, 21 cases in near?mCR group and 3 cases in non?mCR group. The regrowth rate of eCR group was lower than that of mCR group (1/15 vs. 1/8) without significant difference (P=1.000). The regrowth rate of near?eCR group was higher than that of near?mCR group [9/15 vs. 42.9% (9/21)] without significant difference as well (P=0.500). The consistency between colonoscopy and MRI in response evaluation of cCR or near?cCR after nCRT was unsatisfactory ( Kappa=0.341, P=0.011). After initial evaluation, 31 patients underwent watch and wait strategy, and 1 underwent local resection. The 1?and 3?year NR?DFS in the eCR group was both 100%, which was higher than that in the near?eCR group (53.3% and 38.9%, respectively), and the difference was statistically significant (P=0.001). During watch and wait period, 11 cases developed local regrowth by colonoscopy examination and the biopsy result included 4 case of high?grade intraepithelial neoplasia (HIN), 6 cases of adenocarcinoma and 1 case of chronic mucosal inflammation. Meanwhile lateral developmental tumor of ascending colon in 1 case and of sigmoid in a case was found by colonoscopy and confirmed as HIN by postoperative pathology. Besides, 4 cases developed colonic multiple adenoma and all underwent endoscopic resection. Conclusion Colonoscopy examination plays an important role in both initial assessment and regrowth monitoring during watch and wait strategy after nCRT treatment.