Fluid therapy plays a quite important role in peri-operation period,however,conventional measurements are not sensitive to hypovolemia.Goal directed fluid therapy( GDT),which is based on balance of oxygen delivery and consumption,can achieve the aim of fluid therapy more efficiently.Many RCTs have proven that GDT could reduce morbidity,shorten hospital time of high risk surgical patients,therefore,it is a beneficial work.

ObjectiveTo investigate the effect of IL-1? on primary cultured rat hepatocytes (PCRH).MethodsUsing PCRH of Wistar rats, the cytotoxicity of IL-1? (LDH release), the influence of IL-1? on cell proliferation and cellular energy metabolism (intracellular ATP content and KBR) were observed.ResultsIL-1? significantly increased LDH release on all 6 culture conditions with LDH activities of 22?2,25?4,18?5,12?4,15?5 and 11?4, in individual controls compared with 36?3,43?5,34?6,31?4,31?5 and 22?3,in individual IL-1? treated groups, all P

Objective To investigate effects of angiotensin Ⅱ receptor antagonist Losartan on the expression of TGF ? 1mRNA in rats with cirrhosis and the mechanism.Method Forty one male SD rats were divided into normal control ( n =10), cirrhotic control ( n =11), and Losartan treated cirrhotics ( n =20) with Losartan (10?mg?kg -1 ?d -1 ) treatment started on the first day along with CCl 4 administration and on the 5th week, respectively.Expression of TGF ? 1 mRNA was examined with reverse transcriptase polymerase chain reaction (RT PCR).The expression of TGF ? 1 protein was assayed with immunochemistry.Result Expression of TGF ? 1 mRNA and TGF ? 1 protein were up regulated in rats with cirrhosis (from 0 93?0 05 to 1 94?0 13, compared with the normal rats P

Objective To investigate the effect of L osartan, an angiotensin Ⅱ type receptor antagonist, on vascular endothelial gro wt h factor (VEGF) expression. Method Ｚ Liver cirrhosis was in duced by oral intake of 50% CCl4 at a dosage of 3?ml?kg-1?d- 1ⅹ 9 weeks in SD rats. In Losartan group,rats were given Losartan orally. Exp r ession of VEGFmRNA was examined with reverse transcriptase polymerase chain reac tion (RT-PCR) and the expression of VEprotein was assayed by immunohistochem istry. Result VEGF was highly e xpressed in cirrhotic group compared with control group (0.77?0.10 vs 1.94? 0.20, P=0.001) . In losartan treatment group, VEGF mRNA expression decreas ed to 1.02?0.1 and 0.86?0.15（P=0.001）. Concl usion Expression of VEGF increased in cirrhosis, Losartan inhibited this eff ect.

Fine needle aspiration cytopathology of the breast is an effective means of distinguishing malignant from benign,and the aspirated sample collections techniques is the primary and key part of fine needle aspiration.Fine needle aspiration cell block has been improved and used on preoperative breast cancer molecular portraits.This paper will provide an introduction to application of specimens collection techniques,application and development of fine needle aspiration cell block on preoperative breast cancer molecular portraits.

Objective To investigate the effect of platelet activating factor on the proliferation of cultured hepatic stellate cells in vitro. Methods LX-2 cell line was chosen as the study model of the activated hepatic stellate cells. MTT colo- rimetric assay was used to evaluate the proliferation of HSCs. Cell cycle was evaluated by ways of flow cytometry with PI staining and BrdU incorporation.Results OD_(492) value of PAF groups at concentration from (1 × 10~(-8)-1 × 10~(-3) mol/L), were higher than that of control group (P < 0.05). OD_(492) value of PAF (1×10~(-6)) + PAF-RA × 1 (10~(-7)-1 × 10~(-4)mol/L) groups was less than that of PAF (1 ×10~(-6)mol/L) group (P<0.05). S% and PrI of PAF groups increased significantly. Conclusion PAF can increase the proliferation of hepatic stellate cells in a dose-dependent manner while PAF receptor antagonist reduces the proliferation of hepatic stellate cells induced by PAF.

Breast calcification is a specific sign of nonpalpable early breast cancer,the accurate localization and biopsy can obviously improve the diagnosis rate of early breast cancer,and imaging-guided needle localization is the premise of accurate biopsy of breast calcification.As the recommended guidance,mammography-guided localization and biopsy has certain limitations in localizing and biopsying breast calcification,however,high-resolution uhrasound makes it possible to localize the nonpalpable calcification in breast,and which has a certain advantage.

Objective To investigate the different effect of exogenous leptin on estrogen receptor α,β mRNA in human breast tumor tissue in nude mice xenograft models. Methods We made nude mice xenograft models of MCF-7 human breast cancer cells cultured in vitro, then divided them into experimental group of]eptin( n = 30)and control group of normal saline( n = 30)randomly. The models of experimental group were injected subcutaneously the recombinant human leptin for 15 consecutive days, the models of control group were injected subcutaneously the same dose of normal saline. A real- time quantitative RT- PCR assay was developed to quantify the expression of estrogen receptor α, β mRNA in tumor tissue, using the relative quantitative analysis. Results The leptin-intervened nude mice xenograft models were safely established. The relative quantitation of estrogen receptor α mRNA was significantly higher in the leptin group than in the normal saline group ( P ＜ 0.01 ), the relative quantitation of estrogen receptor β mRNA was significantly lower in the leptin group than in the normal saline group ( P ＜ 0. 01 ). Conclusion The nude mice xenograft models can be safely intervened with human leptin by subcutaneous injection around tumor.Estrogen receptor is one of the targets of leptin in the progress of breast cancer. Exogenous human leptin can up- regulate the expression of estrogen receptor α and down- regulate the expression of the estrogen receptor βin nude mice xenograft models of human breast tumor.

Objective To investigate the efficacy and toxicity of neoadjuvant chemotherapy of modified FOLFOX7 regimen in the treatment of advanced gastric cancer. Methods The clinical data of 30 patients with advanced gastric cancer who had been admitted to Beijing Friendship Hospital from June 2005 to January 2009 were retrospectively analyzed. All patients received modified FOLFOX7 regimen [oxaliplatin (100 mg/m~2) + fluorouracil (2400 mg/m~2) + calcium folinate (400 mg/m~2)] for 2-4 courses before operation. The efficacy and toxicity of the modified FOLFOX7 were evaluated before and after the operation. All data were analyzed via chi-square test. Results The pre-and postoperative downstaging rates were 27% (8/30) and 47% (14/30), respectively. The total effective rate of modified FOLFOX7 regimen was 43% (13/30). Complete remission was observed in 1 patient. The toxicity was mild. Radical resection was accomplished in 28 patients (93%). There was no statistical difference between the 2 criteria in evaluating the efficacy of modified FOLFOX7 regimen (χ~2= 2.584, P>0.05). Conclusion Modified FOLFOX7 regimen is effective and well-tolerable for patients with advanced gastric cancer.

Objective This study aimed to establish a prediction system of lymph node metastasis of gastric cancer patients,to facilitate guiding the treatment of patients with gastric cancer.Method s We analyzed 255 cases of gastric cancer from January 2005 to December 2009 in Beijing Friendship Hospital.They all had surgery or palliative gastrectomy and then examined pathological lymph node metastasis.Their gender,age,preoperative weight loss,anemia,pyloric obstruction,chronic disease history,family history,tumor location,tumor size,higher preoperative CEA,preoperative tumor markers (CEA,AFP,CA199,CA125)elevationed (one or more),preoperative choline esterase,preoperative albumin,preoperative hemoglobin,preoperative platelet and preoperative urinary protein were made Logistic regression analysis.Results The combination of preoperative tumor size,higher tumor markers (CEA,AFP,CA199,CA125) in one or more was possible for lymph node metastasis prediction.The area under ROC curve was about 100%,Showing high discriminant ability.Conclusions The tumor size and preoperative tumor markers (CEA,AFP,CA199,CA125) are important predictive parameters for lymph node metastasis of gastric cancer.Tumor size combined with the preoperative tumor markers (CEA,AFP,CA199,CA125) predicting lymph node metastasis can help us to carry out other work,such as neoadjuvant therapy,etc.