The oval nucleus of the bed nuclei of the stria terminalis of the rats was found in a previous investigation be studded with the corticotropin releasing factor (CRF) and neurotensin (NT)-immunoreactive(ir) neurons. The present work was to study their distribution and the possible coexistence of these two neuropeptides in the neuron of oval nucleus. The CRF- and NT- ir neurons were demonstrated to be densely and evenly distributed in the nucleus, among which were scattered a substantial number of cells cocontaining CRF and NT.

Ju and Swanson first named the dorsal part of the anterolateral area of the bed nuclei of the stria terminalis (BST) as oval nucleus (Ov), and found that it was a homogeneous structure rich in neuropeptide-containing neurons and terminals. However, no systemic study has been made about the connections of the Ov.The present experiments were designed to examine the efferent projections of the Ov in the rat. Wheat germ agglutinin conjugated horseradish peroxidase(WGA-HRP) was used as a tracer.Injection of WGA-HRP into the Ov and its adjacent areas resulted in dense anterograde labelling in the posterior part of the lateral hypothalamic area, central nucleus of the amygdala, ventral part of the midbrain central gray, ventral tegmental nucleus, parabrachial nuclei, mesencephalic trigeminal nucleus and locus coeruleus; moderate labelling in the preoptic area, periventricular area of the hypothalamus, arcuate nucleus of the hypothalamus, midline nuclei of the thalamus, medial habenular nucleus, pedunculopontine tegmental nucleus, midbrain reticular formation, dorsal raphe nucleus and the dorsal vagal complex; sparse labelling in the caudal linier nucleus, median raphe nucleus, ventral tegmental area, substantia nigra and intercalated nucleus of medulla oblongata. The results suggest that the Ov may be involved in multiple physiological functions.

The present experiments were designed to study the afferent connections of the oval nucleus (Ov) of the bed nuclei of the stria terminalis (BST) using fluoro-gold (FG) and WGA-HRP as retrograde tracers. After injection of FG or WGA-HRP into the Ov area of the male SpragueDawley rats, a number of retrogradely labeled neurons were observed in the piriform cortex, fundus striati, subiculum, preoptie areas, horizontal limb of the diagonal band, ventral pallidum, magnocellular preoptic area, central (Ce), medial and cortical nuclei of the amygdala. A few neurons in the midline nuclei of the thalamus. were labeled. Many labeled neurons were found in the anterior, dorsal and lateral hypothalamic areas, ventral medial, arcuate and premammillary nuclei of the hypo thalamus. In the brainstem many labeled neurons were seen in the ventral part of the midbrain central gray, dorsal raphe nucleus, pedunculopontine tegmental nucleus, ventral tegmental area, compact zone of the substantia nigra, interpeduncular nuclei, interfascicular nucleus, raphe nuclei, parabrachial nuclei, locus coeruleus, mesencephalie trigeminal nucleus, dorsal vagal complex, A_5 and A_1 cell groups.In general, the distribution patterns of the labeled neurons of both tracers were quite similar except that FG labeled neurons showed much longer processes than WGA-HRP's, and some FG labeled neurons were also found in a few contralateral structures, such as central and medial nuclei of the amygdala and the horizontal limb of the diagonal band, where no WGA-HRP labeled neurons were observed.The results of this retrograde tract-tracing study together with our previous. anterograde tract-tracing study indicate that there are reciprocal connections between the Ov and the Ce, posterior part of the lateral hypothalamus and several brainstem structures.

In our recent work it was found that corticotropin-releasing factor (CRF) and neurotensin(NT) were coexistent in the neurons of the oval nucleus (Ov) of the bed nuclei of the stria terminalis (BST) and there were reciprocal connections between the Ov and the lateral hypothalamic area(LHA).In the present experiments fluoro-gold (FG) retrograde tract-tracing technique combined with indirect immunofluorescence staining was used to investigate the chemical properties of the Ov neurons projecting to the LHA in the male Sprague-Dawley rats. It was found that after injecting of FG into the posterior LHA the retrogradely labeled neurons on the Ov were positively stained with antisera to CRF or NT. The present study proves that a part of CRF- and NT-containing neurons in the Ov project to the posterior LHA.

The central nucleus of the amygdala(Ce) is heterogeneous in cyto- and chemoarchitecture, and contains over a dozen neuropeptides. Our recent works suggested that there were reciprocal connections between the Ce and the oval nucleus (Or) of the bed nuclei of the stria terminalis (BST). The present experiment was designed to study the immunocytochemical properties of the Ce neurons projecting to the Ov by the fluoro-gold(FG) retrograde tract-tracing method combined with indirect immunofluorescence staining in male Sprague-Dawley rats.It was found that the neurons in the Ce were labeled densely following injection of FG into the Ov, and some FG labeled neurons were restained by indirect immunofluorescence procedures using antisera to corticotropin-releasing factor (CRF), neurotensin (NT), M-enkephalin(M-ENK) or cholecystokinin(CCK). The findings suggest that the CRF-, NT-, M-ENK- and CCK-containig neurons in the Ce project to the Ov.

Objective To explore short-term effect of tonsillotomy on children′s obstructive sleep apnea hypopnea syndrome (OSAHS) with tonsil hypertrophy by Ellman double-frequency radio-frequency machine .Methods Totally 30 cases children′s OS-AHS with tonsil hypertrophy were had tonsillotomy by Ellman double-frequency radio-frequency machine .They were compared with 30 cases children′s OSAHS with tonsil hypertrophy by traditional tonsillectomy .Short-term effect of tonsillotomy were ana-lyzed by wound bleeding ,operation time ,postoperative pain ,difficulty eating ,PSG testing ,immune function ,growth and develop-ment .Results Postoperative symptom in 1 weeks and 6 months ,compared with the traditional tonsillectomy ,30 cases of OSAHS patients were found that there were less bleeding ,shorter operative time ,mild postoperative pain ,no difficulty in feeding ,improving sleep-disordered breathing ,no decline in immune function ,good growth and development .Conclusion Short-term effect of tonsillot-omy on children′s OSAHS with tonsil hypertrophy is good by Ellman double-frequency radio-frequency machine ,it is a minimally invasive ,safe and effective surgical method children OSA HS .

Objective To study the effect of human ?-globin matrix attachment region(MAR) on transgene expression in stably transfected CHO cells.Methods Expression vector was constructed,which contained the ?-globin MAR in both sides of Chloramphenicol acetyltransferase(CAT) reporter gene expression cassette in cis,then transfected into CHO cells.The CAT reporter gene expression was analyzed by ELISA method.Results The ?-globin MAR enhanced the CAT gene expression 5.5-fold in stably transformed CHO cells,while the transgene expression variation among individuals of transformants was decreased.Conclusion MAR increase transgene expression in stably transfected CHO cells.

potential use in clinical therapy and prognosis estimation of the tumor.

Objective To predict the secondary structure and the B cell epitopes of human heparanase protein, and to identify its immunogenicity. Methods The flexible regions of secondary structure and the B cell epitopes of human heparanase amino acid sequence were predicted by DNAStar and Bcepred software. The multiple antigenic peptides (MAP) of the epitopes were synthesized in 8-branch form. Rabbits were immunized with the 8-branch MAPs mixed with a universal T-helper epitope human IL-1β peptide (VQGEESNDK, amino acid 163-171 ). The immunogenicity of the synthesized peptides was evaluated by ELISA, Western blot and immunohistochemistry. Results Amino acid 1 -15 ( MAP1), 279-293 (MAP2) and 175-189(MAP3) of large-subunit of human heparanase protein was predicted as the most potential epitopes of human heparanase protein. All the three synthesized MAPs induced high titer of antibodies. ELISA, Western blot and immunohistochemistry analysis showed all the three MAPs could produce high titer serum antibodies, antibodies induced by MAP1 and MAP2 had high specific binding activity , and MAP2 antibody showed the strongest binding activity with liver cancer tissues. Conclusion The large-subunit No. 1-15, 279-293 amino acid of human heparanase protein may be the B cell preponderant epitopes and the strongest immunogenicity may be No. 279-293 peptide, which provided a theoretic basis for the antibody and vaccine development of heparanase subunit peptide.

OBJECTIVETo investigate the association of neuroendocrine differentiation with progression and prognosis of gastric adenocarcinoma.

METHODSClinicopathological data of 240 patients with gastric adenocarcinomas were retrospectively analyzed. The expression of chromogranin A, synaptophysin and secrectagogin in cancer tissue was assessed by immunohistochemistry. The association of neuoroendocrine differentiation parameters with disease progression and survival of patients was analyzed.

RESULTSThe expression of synaptophysin was positively correlated with depth of invasion and secretagogin more often expressed in cases with lymph node metastasis. In Lauren diffuse type of cancer, expression of chromogranin A and secretagogin was unfavorable prognostic predictor. In TNM stage II adenocarcinoma, expression of chromogranin A and synaptophysin related to poor survival, and multivariate Cox proportional hazard model showed that synaptophysin was an independent predictor for poor survival.

CONCLUSIONNeuroendocrine differentiation predicts deeper depth of invasion, more possibility of lymph node metastasis and poor survival in gastric adenocarcinoma.

Adenocarcinoma ; diagnosis ; pathology ; Biomarkers, Tumor ; metabolism ; Chromogranin A ; metabolism ; Disease Progression ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Neoplasm Staging ; Neuroendocrine Tumors ; diagnosis ; pathology ; Prognosis ; Proportional Hazards Models ; Retrospective Studies ; Secretagogins ; metabolism ; Stomach Neoplasms ; diagnosis ; pathology ; Synaptophysin ; metabolism