Objective To analyze the effect and clinical value of health behavior intervention on postoperative hope level and negative emotional in patients with lung cancer surgery.Methods From December 2014 to October 2017,170 cases with lung cancer in Taizhou Hospital of Zhejiang were selected.According to the intervention methods,85 cases were divided into control group and observation group.The patients in the control group were treated with routine nursing,the observation group received routine nursing combined with healthy behavior intervention nursing mode,and the nursing effect of the two groups was analyzed.Results After intervention,the anxiety self-rating scale score,depression self-rating scale score in the observation group were(45.43 ±3.08)points,(46.02 ±3.45)points,respectively,which in the control group were(50.02 ±6.62)points,(53.65 ±6.31)points,respectively,and the differences between the two groups were statistically significant(t=5.287,5.187,all P<0.05).After intervention,the positive attitude,positive action,and keep close relationship with others in the observation group were(13.04 ±2.88)points,(12.99 ±2.97)points,(13.23 ±2.96)points,respectively,which in the control group after intervention were(10.02 ± 1.71)points,(10.13 ±1.86)points,(10.09 ±1.69)points,the differences between the two groups were statistically significant(t=4.871,5.017,4.418,all P<0.05).After intervention,the scores of physical function,role function,emotional function,cognitive function,social function,fatigue,nausea and vomiting,pain in the observation group were(74.04 ±7.73)points,(76.99 ±6.34)points,(75.01 ±7.86)points,(73.76 ±8.54)points,(75.92 ± 6.18)points,(77.98 ±7.08)points,(79.32 ±8.32)points,(72.11 ±7.18)points,respectively,which were higher than those in the control group,the differences were statistically significant(t=11.387,10.386,12.487,11.672,13.987,9.438,14.687,10.438,all P<0.05).Conclusion The application of healthy behavior intervention in patients with lung cancer can reduce anxiety and depression negative emotions,improve the hope level,improve the quality of life,and deserve to be applied in clinical application.

Objective To study the mutation of FLCN gene in Chinese patients with sporadic and familial primary spontaneous pneumothorax. Methods A complete genetic analysis of FLCN by use of SSCP-PCR was performed in 102 unrelated Chinese patients with isolated PSP. Results Three novel mutations (c. 924_926del, c. 1611_1631del and c. 1740C.T) and a previously reported mutation (c. 1733insC) were identified in five familial and five sporadic PSP patients. Of the 21 family members of patients with PSP including 3 previous considered as sporadic, 4 ( 19% ) had history of at least one episode of PSP and 9 (43% ) were FLCN mutant carriers without PSP. Seven of the nine (78% ) mutant carriers had pulmonary cysts detected by high-resolution computed tomography ( HRCT) . Although c. 924_926del and c. 1611 _1631 del were found in eight patients from the same geographic district, haplotype analysis demonstrated that they did not share the same affected haplotype,thus excluding common ancestry. Conclusion This study first demonstrates that FLCN mutation contributes to not only familial but also apparently sporadic' patients with isolated PSP. It suggests that mutation analysis and HRCT scan may be recommended for first-degree family members of PSP patients with FLCN mutations, irrespective of their family history status of PSP.

In order to overcome the challenges of insufficient restriction enzyme sites, and construct a fusion-expression vector with flexible fusion direction, we designed an LB cloning system based on the type IIS and type IIT restriction enzymes LguⅠ and BbvCⅠ. The LB cloning system is constructed by inserting the LB fragment (GCTCTTCCTCAGC) into the multiple cloning site region of the broad-host plasmid pBBR1MCS-3 using PCR. The LB fragment contains partially overlapped recognition sites of LguⅠ and BbvCⅠ. Therefore, the same non-palindromic sequence will be generated by these two restriction endonucleases digestion. This feature can be used to quickly and flexibly insert multiple genes into the expression vector in a stepwise and directed way. In order to verify the efficacy of the cloning system, two glycosyltransferase genes welB and welK of Sphingomonas sp. WG were consecutively fused to the LB cloning vector, and the recombinant plasmid was transferred into Sphingomonas sp. WG by triparental mating. The results showed that gene fusion expression has little effect on sphingan titer, but enhanced the viscosity of sphingan. The viscosity of the sphingan produced by recombinant strain Sphingomonas sp. WG/pBBR1MCS-3-LB-welKB was 24.7% higher than that of the wild strain after fermentation for 84 h, which would be beneficial for its application. In conclusion, the application of LB cloning system were verified using Sphingomonas sp. WG. The LB cloning system may provide an efficient tool for fusion expression of target genes.
Base Sequence ; Cloning, Molecular ; Fermentation ; Plasmids/genetics* ; Sphingomonas/metabolism*