Objective: To study the chemical constituents in the barks and leaves of Cinnamomum cassia.Methods: The crude extracts of the barks and leaves of Cinnamomum cassia were extracted by ethanol, the chemical constituents of the crude extracts were separated and purified by chromatographic methods and identified by spectroscopic analysis.Results: Five lignans were separated from the barks and two lignans were separated from the leaves of Cinnamomum cassia as follows: (7S,8R)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (1), 1,2,3-propanetriol,1--3-methoxyphenyl-(1R,2R) (2), (6R,7R,8R)-7a-lyoniresinol (3), (6S,7R,8R)-7a-lyoniresinol (4), (6R,7S,8S)-7a-lyoniresinol (5),(+)-lariciresinol(6), (-)-4-epi-lyoniresinol (7).Conclusion: All the lignans are isolated from Cinnamomum cassia for the first time.

In this research a strain of isolated Pseudomonas alcaligenes which causes degradation of dexamethasone was acclimated further and its proteins of every position in the bacterium were separated by the osmotic shock method. The separated intracellular proteins which had the highest enzyme activity were extracted by the salting out with ammonium sulfate and were purified with the cation exchange chromatography and gel chromatography. The purified proteins which was active to cause degradation of dexamethasone had been detected were cut with enzyme and were analyzed with mass spectrometry. The results showed that the degradation rate to dexamethasone by acclimated Pseudomonas alcaligenes were increased from 23.63% to 52.84%. The degrading enzymes were located mainly in the intracellular of the bacteria and its molecular weight was about 41 kD. The specific activity of the purified degrading enzymes were achieved to 1.02 U x mg(-1). Its 5-peptide amino acid sequences were consistent with some sequences of the isovaleryl-CoA dehydrogenase. The protein enzyme may be a new kind degrading enzyme of steroidal compounds. Our experimental results provided new strategies for cleanup of dexamethasone in water environment with microbial bioremediation technique.
Amino Acid Sequence ; Bacterial Proteins ; chemistry ; Chromatography ; Dexamethasone ; chemistry ; Molecular Weight ; Pseudomonas alcaligenes ; enzymology

@@

Objective To summarize the assessment standards of organ donation after cardiac death (DCD) donor lungs application and donor lung function maintenance experience.Method From Jan.2013 to June 2015,139 cases of DCD donors were subjected to rigorous assessment and effective donor lung function maintenance,and 11 donor lungs for lung transplantation were obtained.The donor lung cold ischemia time was (526.8-± 12.6) min (312 to 675 min).Double lung transplantation was performed on 9 cases,and 2 cases received single lung transplantation.Result Perioperatively,1 lung transplant recipient died of pulmonary infection.The survived 10 recipients had no rejection after operation,and obtained good quality of life during discharge to the final follow-up.Condusion The effect of donor lung transplantation using organ donation is satisfactory.The assessment standards and functional maintenance of donor lung are important factors to guarantee the success of lung transplantation.

Objective:To investigate the effects of different deoxyepinephrine administration methods on hypotension in patients undergoing thoracoscopic general anesthesia.Methods:A total of 120 patients undergoing thoracoscopic general anesthesia in Jinhua Municipal Central Hospital from January 2020 to January 2023 were included in this study. They were randomly divided into a control group and an observation group ( n = 60 patients per group). Patients in the control group were given deoxyepinephrine once, and patients in the observation group were continuously pumped with deoxyepinephrine. Other anesthesia methods in the two groups were the same. Hemodynamic indexes (systolic blood pressure, diastolic blood pressure, and heart rate), incidence of hypotension (during anesthesia induction and operation), anesthesia time, operation time, urine volume, blood loss, infusion volume, and dosage of deoxyepinephrine were recorded. Results:At the time at which the first systolic blood pressure decreased by > 20% of the baseline (T1) and 5 minutes after T1 (T2), the systolic blood pressures in the observation group were significantly higher than those in the control group [T1: (99.77 ± 11.42) mmHg vs. (95.34 ± 15.37) mmHg, t = 1.79, P = 0.038; T2: (120.49 ± 12.48) mmHg vs. (113.45 ± 16.03) mmHg, t = 2.68, P = 0.004; 1 mmHg = 0.133 kPa]. At T1 and T2, diastolic blood pressures in the observation group were significantly higher than those in the control group [T1: (62.60 ± 5.81) mmHg vs. (59.43 ± 6.35) mmHg, t = 2.85, P = 0.003; T2: (73.61 ± 7.01) mmHg vs. (70.20 ± 8.15) mmHg, t = 2.46, P = 0.008]. The incidences of hypotension during anesthesia induction and surgery in the observation group were 8.33% (5/60) and 15.00% (9/60), respectively, which were significantly lower than 26.67% (16/60) and 33.33% (20/60) in the control group ( χ2 = 6.98, P = 0.008; χ2 = 5.50, P = 0.019). There were no significant differences in anesthesia time ( t = 0.19, P = 0.425) and operative time ( t = 0.27, P = 0.396) between the two groups. There were no significant differences in urine volume ( t = 0.92, P = 0.179), blood loss ( t = 1.02, P = 0.155), and infusion volume ( t = 0.91, P = 0.182) between the two groups. There was no significant difference in the dosage of deoxyepinephrine between the two groups ( t = 1.11, P = 0.134). Conclusion:Continuous infusion of deoxyepinephrine in patients undergoing thoracoscopic general anesthesia yields superior efficacy than a single administration of deoxyepinephrine. The former can stabilize the hemodynamic indicators of patients during surgery and reduce the incidence of hypotension.

Objective:To investigate the effect of penehyclidine hydrochloride on respiratory mechanics, arterial blood gas, and inflammatory factors in patients undergoing one-lung ventilation during thoracoscopic lobectomy.Methods:A total of 100 patients who underwent thoracoscopic lobectomy with one-lung ventilation at Jinhua Central Hospital from January to November 2022 were included in this randomized controlled study. They were divided into groups A and B ( n = 50 per group) using a random digital number table. Patients in group A received an intravenous infusion of 0.02 mg/kg of pentylenethyclidine hydrochloride 30 minutes before surgery, while patients in group B received an equal amount of 0.9% sodium chloride injection 30 minutes before surgery. Clinical indicators, respiratory mechanical indicators (peak airway pressure, lung compliance), arterial blood gas analysis indicators (blood oxygen saturation, arterial pressure of oxygen, oxygenation index), inflammatory factor levels (interleukin-6, interleukin-8, tumor necrosis factor level-α), and pulmonary complications were compared between the two groups. Results:There were no significant differences in mechanical ventilation time or total infusion volume between the two groups (both P > 0.05). At the end of surgery (T1) and 1 day after surgery (T2), peak airway pressure in group A was (17.43 ± 2.69) cm H 2O and (16.81 ± 2.28) cm H 2O (1 cm H 2O = 0.098 kPa), respectively, which were significantly lower than (19.23 ± 3.40) cm H 2O and (18.29 ± 2.06) cm H 2O in group B, respectively ( t = 2.94, 3.41, P = 0.002, < 0.001). At T1 and T2, lung compliance in group A was (34.67 ± 2.93) cm H 2O and (36.26 ± 3.11) cm H 2O, respectively, which were significantly higher than (32.23 ± 2.85) cm H 2O and (33.84 ± 2.87) cm H 2O in group B, respectively ( t = 4.22, 4.04, P = 0.000, < 0.001). At T1 and T2, blood oxygen saturation, arterial partial pressure of oxygen, and oxygenation index in group A were significantly higher than those in group B ( t = 8.12, 3.07, 10.47, 3.16, 3.81, 4.15, all P < 0.05). At T1 and T2, interleukin-6, interleukin-8, and tumor necrosis factor-α levels in group A were significantly lower than those in group B ( t = 11.67, 13.55, 9.60, 15.71, 6.13, 11.50, all P < 0.001). The incidence of complications in group A was 4% (2/50), which was significantly lower than 16% (8/50) in group B ( χ2 = 4.00, P < 0.05). Conclusion:Penehyclidine hydrochloride has a good effect on respiratory mechanics, arterial blood gas, and inflammatory factors in patients undergoing thoracoscopic lobectomy with one-lung ventilation and thereby deserves clinical promotion.

OBJECTIVE: To investigate the expression of microRNA miR-431-5p in gastric cancer (GC) tissues and its effects on apoptosis and mitochondrial function in GC cells. METHODS: The expression level of miR-431-5p in 50 clinical samples of GC tissues and paired adjacent tissues was detected using real-time fluorescence quantitative PCR, and its correlation with the clinicopathological features of the patients was analyzed. A cultured human GC cell line (MKN-45 cells) were transfected with a miR-431-5p mimic or a negative control sequence, and the cell proliferation, apoptosis, mitochondrial number, mitochondrial potential, mitochondrial permeability transition pore (mPTP), reactive oxygen species (ROS) production and adenosine triphosphate (ATP) content were detected using CCK-8 assay, flow cytometry, fluorescent probe label, or ATP detection kit. The changes in the expression levels of the apoptotic proteins in the cells were detected with Western blotting. RESULTS: The expression level of miR-431-5p was significantly lower in GC tissues than in the adjacent tissues (P < 0.001) and was significantly correlated with tumor differentiation (P=0.0227), T stage (P=0.0184), N stage (P=0.0005), TNM stage (P=0.0414) and vascular invasion (P=0.0107). In MKN-45 cells, overexpression of miR-431-5p obviously inhibited cell proliferation and induced cell apoptosis, causing also mitochondrial function impairment as shown by reduced mitochondrial number, lowered mitochondrial potential, increased mPTP opening, increased ROS production and reduced ATP content. Overexpression of miR-431-5p significantly downregulated the expression of Bcl-2 and increased the expressions of pro-apoptotic proteins p53, Bcl-2 and cleaved caspase-3 protein. CONCLUSION The expression of miR-431-5p is down-regulated in GC, which results in mitochondrial function impairment and promotes cell apoptosis by activating the Bax/Bcl-2/caspase3 signaling pathway, suggesting the potential role of miR-431-5p in targeted therapy for GC.
Humans ; Apoptosis/genetics* ; bcl-2-Associated X Protein ; Caspase 3 ; Cell Line, Tumor ; Cell Proliferation/genetics* ; MicroRNAs/metabolism* ; Mitochondria/metabolism* ; Mitochondrial Permeability Transition Pore ; Reactive Oxygen Species ; Stomach Neoplasms/pathology*