Hemoperfusion ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy

In peroneal nerve-anterior tibialis muscle preparations of uretbane anesthetized rabbits, sodium ammonium dimethyl-2 -( propano-1 , 3 - dithiosulfonate ) monohydrate ( SCD ) 7 .5mg/kg iv depressed the antogenous respiration completely and the indirectly elicited twitch tension completely finally. Sodium 2, 3 - dimercaptopropane- 1-sulfonate ( DMPS ) 7、 10mg/kg iv,respectively completely antagonized the respiratory depression and the neuromuscle block, DMPS (2.4、 3.4、 4.9mg/kg, iv ) could antagonize these toxic effects partly. DMPS (62.5mg/kg ,iv)could antagonize the toxic symptom induced by SCD(7.5 mg/kg, iv)in rabbits. At dose level higher than 12.5mg/kg of SCD, this toxin caused tremor and tonic convulsion. e Wfound DMPS (62.5 mg/kg, iv ) combined with diazepam ( 5mg/kg, iv ) has antidotal effects on acute poisoning caused by SCD in rabbits.

Objective To observe the ultrastructure of blood-brain barrier (BBB) around hematoma in patients with hypertensive intracerebral hemorrhage (ICH).Methods Brain tissue around hematoma in 10 patients with hypertensive ICH was obtained using stereotaxic apparatus targeted by CT scan. Ultrastructure of BBB was observed under electron microscope. Results Astrocytic end feet swelled and pinocytotic vesicles increased 12 h after ICH. Mitochondrion of endothelial cells swelled and ridges disrupted 24 h after ICH. Astrocytic end feet and endothelial cells showed apparent edema, cell nuclei tumefied 48 h after ICH. Cytoplasm loosened and fundus membranes ruptured 72 h after ICH. Endothelial cell membranes and nuclei dissolved and capillary vessels dented 5 d after ICH. Conclusion Prevention of BBB from damage is important in treating ICH at early stage.

Background Human Tenon fibroblasts (HTFs) are the main cell component in scar tissue after antiglaucomatous filtrating surgery.To study the in vitro growth and proliferation features will offer an approach to the preventing and treating of scarring following antiglaucomatous filtrating surgery.Objective The goal of present study was to investigate the growth characteristics of HTFs in vitro from patients with bleb scarring after antiglaucomatous filtrating surgery.Methods This study process was approved by Ethic Committee of Beijing Chaoyang Hospital,and informed consent was obtained from each subject prior to the initaion of the study.The specimens of scaring tissue were obtained during the secondary trabeculectomy from 8 eyes with bleb scaring after initial antiglaucomatous filtrating surgery with the tissue size of 4 mm×5 mm,and the normal Tenon capsule specimens were acquired during the strabismus surgery from 8 eyes in the same way.HTFs were primarily cultured and passaged by tissue explants adherent method and identified using immunoinfluorescence technique with vemintin antibody.Nest PCR was used to exclude the mycoplasma infection during the culturing process.The morphology,growth curve and population doubling time (PDT) of the fifth generation of cells were examined and calculated by luminescence method cell vitality method 0,4,7,11,14 days and compared between the patients and normal groups.The stability of the cell growth was assessed by comparing the morphology,growth curve and PDT between the fifth generation and fifteenth generation of HTFs.Results Primarily cultured cells reached confluence 1-2 weeks with the similar shape to HTFs.The growth properties of the cells were same between the scarring group and normal group and showed positive response for vemintin antibody.No react band for mycoplasma was detected.The PDT was (20.54±3.51) hours and (18.86±2.91) hours in the scarring group and normal group,respectively,showing insignificant difference between them (t=0.634,P=0.561).No significant differences were found in the number of cells in 4,7,11 and 14 days after passage (t =2.663,P =0.081; t =0.194,P =0.863 ; t =3.338,P =0.053 ; t =0.627,P =0.565),with a consistent growth curve with the lapse of time between the two groups.The HTFs from scarred Tenon capsule fibrosis were cultured and passaged until the fifteenth generation.The PDT was gained to be (20.54 ±3.51) hours in the fifth generation of cells and (22.84±4.15) hours in the fifteenth generation of cells,without significant difference between them (t =0.733,P =0.505).The number of cellsin 4,7,11 and 14 days was not significantly different between the fifteenth generation and the fifth generation of cells (t=1.528,P=0.235;t=0.269,P=0.786;t=1.954,P=0.139;t =0.730,P =0.506).In addition,a good and stable growth curve also was exhibited in the fifteenth generation of cells compared with the fifth generation of cells.Conclusions Bleb scar-derived HTFs present good and stable growth in vitro.This result demonstrates HTFs were target cells in antifibrosis study after antiglaucoma filtrating surgery.

Objective We conducted an in vivo study with the mouse model of Vib rio vulnificus infection to evaluate the efficacies of combination therapy with antimicrobia l agents. Methods Vibrio vulnificus (6.0?10 8 cfu/ml)was injected intraperitoneally into the right abdominal cavity. One hour, 2 hour and 3 hour after inoculation, 7 antimicrobial agents were given alone or in combination intraperitoneally at human therapeutic dose level. The numbers of survial mouse and the supermicrostr ucture change of organs w ere observed. 7 antimicrobial agents were Imipenem, Chloramphenicol, Doxycycline Hydrochloride, Netilmicin, Cefoperazone, Piperacillin, Levofloxacin. Res ults Two hours after infection, the mouse survival rates of groups trea ted with Chloramphenicol, Levofloxacin, Netilmicin, Cefoperazone was 100%. Howev er, the survival rates in the mouse treated by Piperacillin was 60% and the surv ival rate of in the mouse treated with Imipenem or Doxycycline Hydrochloride was 20%. The survival rate in the mouse treated with Cefoperazone combined with Lev ofloxacin, Cefoperazone combined with Netilmicin, or Netilmicin combined with Do xycycline Hydrochloride, was 100%. The supermicrostructure injure of the organs in the mouse recovered. Conclusions These results indicate that Chloramphenicol, Netilmicin, Cefoperazone and Levofl oxacin alone had satisfactory efficacy in the treatment of experimental Vibrio v ulnificus infection in mouse. The combination therapies of Cefoperazone with Lev ofloxacin, Cefoperazone with Netilmicin, and Netilmicin with Doxycycline Hydroch loride are more advantageous than using antimicrobial agent alone.

5 minutes and the emergency room documentation of Glasgow Coma Scale of ￡4,arterial pH of ≤7.0 all predicted bad outcome,with a statistical significance(P

Objective To investigate methods of weaning acute brain stem hemorrage patients with respiratory failure from mechanical ventilation.Methods This was a retrospective analysis of 45 acute brain stem hemorrage patients with respiratory failure requiring mechanical ventilaiton.Results 15(33.3%)patients were successfully weaned from mechanical ventilation for the first time;the other 30(66.7%)patients failed in the first place,then succeeded after 3~7 days of and occasional disconnection from ventilation.The time of mechanical ventilation varied from 6 to 35 days.Conclusion It is difficult to wean acute brain stem hemorrage patients with respiratory failure from mechanical ventilation.Occasional disconnction from ventilaiton,antiinfection and nutritional supplementation might contribute to successful weanning.

Objective To study the effects of naloxone on ?-endorphin (?-EP) in plasma and on myocardial ultrastructure during myocardial ischemia-reperfusion (I/R) injury in rabbits.Methods The myocardial ischemia models and myocardial ischemia -reperfusion injury models in rabbits,by ligating the left anterior descending branch of coronary artery,were used to investigate the changes of ?-EP and ET-1 in plasma during I/R injury, and after treatment with naloxone, an antagonist of opiate receptor. 20 New Zealand rabbits were randomly assigned to 2 groups (each 10 rabbits in naloxone treatment and ischemia-reperfusion group). The bloods were taken at different times in each group. The concentrations of ?-EP and ET-1 were detected with radioimmunology method.The changes of myocardial ultrastructure in samples of myocardial ischemia,were observed through electron microscope.Rseults The levels of ?-EP were significantly improved after I/R injury compared with those before ischemia (P0.05). For ischemia-reperfusion group, most of cardiac muscles were in the contracting state, the myofibril dissolved and broke locally. The edema was found on the circumference of nucleus. For naloxone treatment group, the structure of myofibril was clear and its arrangement was in good order, and no obvious breakage was found. The contracting of myofibril, the edema of mitochondria and kytoplasm were moderating. Conclusion Naloxone may effectively control the levels of ?-EP and the synthesis and secreting of ET-1 after myocardial ischemia and during I/R injury;and reduce the injury to the myocardial ultrastructure and decrease the injury to blood vessel and myocardium.

Objective To analyze and summarize the clinical diagnosis and therapy features of chronic liver disease patients with limbs infection.Methods A retrospective analysis was performed on 29 chronic liver disease patients with serious limbs infection in our hospital.Results Chronic liver disease patients with limbs infection specially vibrio infection,had a high ratio of MODS,so early diagnosis,early therapy with antibiotics and early operation to expose and cut lesion has a good effect.Conclusions The chronic liver disease patients with limbs infection should early diagnosed,early treated by antibiotics,and early exposed the swelling limbs or cut lesion by surgery.