Objective Because in the environment there are many compounds to cause variation of cells, the study for high sensitivity’s method in which the variation of cells by environment can be tested is necessary. For example, the reports of detailed study for the application of cadmium on the human body cell’s lethal toxicity and the variation of cells are very few. This research is about a high sensitivity method of heavy metal cadmium’s variation active processed by cadmium in which the variation of cells can be tested by ultraviolet ray. Methods The study used cultured cells of human being and derivation. The assay of mutagenicity OuaR (Ouabain resistance) variation used picking law and Differential dot-blot of cancer gene K-ras variation the hybridization examination method. In order to test the induction’s frequency of cell variation, MTT (methyl thiazolyl tetrazoliun) and survival efficiency of formation were tested. The clarifing of mutagenicity mechanism used Western blot and the siRNA method to analysis the molecular of heat shock protein. Results Cadmium clarifiing mutagenicity variation detection had the same degree with the method of ultraviolet ray. The DNA was injury corresponding to the HSP27 protein cell’s change.The cell variation frequency also presented the high value. Conclusion Human fibroblasts in the role of the heavy metal cadmium showed that HSP27 protein expression in high-UVr-1 cells RSa has lowor-frequency variations than OuaR cells. K-ras dot blot method proved that RSa cells variation is higher than the cells UVr-1 mutation. In siHSP27 cells, we observed OuaR high variability of expression. Cadmium on DNA damage showns UVr-1 fibroblast cell is lower than RSa fibroblasts cell.

Objective To analyze the radiolngical findings of mediastinal ganglioneuroma and to improve its diagnostic accuracy. Methods Imaging data of 8 cases of pathologically proven mediastinal ganglioueuroma were restrospectively analyzed. Results These tumors could occur in the anterior mediastinum, middle mediastinum or posterior mediastinum, with a preference for the posterior mediastinum (6/8). No specific clinical symptoms and signs were observed. Well-defined enlargrment of mediastinum with homogeneous density was shown on plain X-Ray. CT scanning was performed in 7 cases, including non-contrast scan alone (n = 3 ), both non-coutrast and contrast-enhanced scans ( n = 4). Round or oval shaped, well circumscribed, homogeneous or slightly heterogeneous, hypadense masses were demonstrated on non-contrast scan. Spotty calification could be found in a few cases. Homogeneous or slightly heterogeneous enhancement was seen following the intravenous injection of contrast material. Large tumors showed a tendency of wedging into the space between adjacent organs and structures, and encasing the nearby large vessels. MR without contrast was performed in 1 case. T1 WI showed isointensity to adjacent muscle, T2WI showed homogeneous hyperintensity. Multi-planar reconstruction provided more information concerning the relationship of the mass lesions with neighboring structures. Conclusion Mediastinal ganglioneuromas have some specific characterstics on imaging studies, which could assist in pre-operative diagnosis and surgical planning.

Objective: To explore the forces of lower limbs during side slip and forward slip through biomechanical study, and to provide a theoretical basis for the development of lower limb movements. Methods: BTS SMART DX motion capture analysis system, 8 infrared high-speed cameras, Kistler three-dimensional dynamometer, BTS FREEEMG 1000 portable wireless surface electromyography system were used to collect real-time data of side slip and forward slip. SPSS 20.0 univariate analysis of variance (ANOVA) was used to compare the differences of indicators between different groups. Results: In the side sliding movement, there were significant differences in hip motion (P<0.01) between 3-year-old group and 5-year-old group, hip motion(P<0.01) between 4-year-old group and 5-year-old group, knee motion(P<0.05) and ankle motion(P<0.05) between 3-year-old group and 5-year-old group. There was a significant difference in hip motion(P<0.05) between 3-year-old group and 5-year-old group. The Fx force(P<0.01) and vertical Fy force(P<0.01) in the left and right directions of the 3-year-old group and the 5-year-old group were significantly different in the side-slip movement, while the Fx force(P<0.01) in the right and left directions of the 3-year-old group and the 5-year-old group were significantly different in the forward-slip movement, and the vertical Fy force(P<0.05) was significantly different. There were significant differences in the long head of biceps femoris, gastrocnemius and anterior tibial muscle between 3-year-old group and 5-year-old group (P<0.05). There were significant differences in the middle gluteal muscle, gracilis muscle, adductor magnus, pubic muscle, medial femoral muscle and soleus muscle between 3-year-old group and 5-year-old group (P<0.05). There was significant difference in the anterior tibial muscle (P<0.05) between the 3-year-old group and the 5-year-old group in the supporting stage, and significant difference in the gastrocnemius muscle(P<0.05) during the swing stage. There was significant difference in the anterior tibial muscle(P<0.05) between the 3-year-old group and the 5-year-old group in the swing stage. Conclusion The development of side slip and forward slip in children aged 3 to 5 years old showed that the results suggest motor development increases significantly with age among preschoolers. Rhythmic movements of side slip and forward slip should be selected in the critical period of children’s movement development at different ages, laying the foundation for good physical and mental development.

Objective:The regional emergency system needs to maintain normal operation in disasters and sudden public health events, ensuring the timely treatment of critically ill patients, and also bearing the responsibility of ensuring various large-scale sports events. This article explores the practice of the "three-dimensional closed-loop" emergency system in the guarantee of the Hangzhou Asian Games in Wenzhou.Methods:After analyzing and integrating existing medical resources, the First Hospital Affiliated to Wenzhou Medical University conducted a two-year medical emergency exercise in a simulated urban road and natural water environment, and improved and optimized the weak links in the medical security system.Results:The hospital established a "three-dimensional closed-loop" emergency system and completed the medical support tasks for the Asian Games. The "three-dimensional closed-loop" emergency rescue system adopted a three-dimensional management team that combined a decentralized organizational structure and a professional medical team, and also has a land-air-sea emergency rescue team. By combining manpower and information technology, the system could provide "4R" medical services: real-time perception of medical emergencies in venues, rapid assessment of the patient's condition, rapid transfer of patients and rapid deployment of medical forces within the hospital. In addition, closed-loop management of the hospital's diagnosis and treatment process improved patients' satisfaction during the treatment process.Conclusions:The the "three-dimensional closed-loop" emergency system provided a strong guarantee for the successful holding of the Hangzhou Asian Games in Wenzhou and also accumulates experience for future urban emergency rescue.

Objective:To investigate the status and influencing factors of health-promoting lifestyle in young and middle-aged people with high normal blood pressure, so as to provide the basis for primary prevention about hypertension.Methods:From July to December 2020, a convenience sampling method was used to select 280 cases of young and middle-aged people with high normal blood pressure in the First Affiliated Hospital of Wenzhou Medical University were investigated by self-administered general information questionnaire,and Health Promotion Lifestyle Profile Ⅱ(HPLP-Ⅱ).Results:The HPLP-Ⅱ total score in the young and middle-aged with high normal blood pressure was 146.79 ± 29.57. Multiple linear regression analysis showed that different occupation,income, family history of hypertension and learning-willingness were the influencing factors of their health-promoting lifestyle( P<0.05). Conclusions:The health-promoting lifestyle among the young and middle-aged with high normal blood pressure is at a medium level, health care providers should strengthen guidance to improve their health-promoting lifestyle through health education and other ways for individuals without stable employment, poor income level, no family history of hypertension and lack in willingness to learn.

Objective To compare the biomechanical characteristics of lower limbs of young people with different body mass index (BMI) before and after the intervention of Baduanjin exercise, so as to provide references for scientific exercise and sport prescription for young Baduanjin practitioners with different BMIs. Methods Young volunteers with different BMI were divided into standard group, overweight group and obesity group. The BTS SMART 3D infrared motion capture system was used to capture the motion parameters and electromyography (EMG) parameters, the KISTLER dynamometer was used to collect the dynamic parameters, the AnyBody simulation software was used to calculate the kinematic parameters and dynamic parameters, and BTS SMART Analyzer was used to compare the different integral electromyography values. Results There was no significant difference in each parameter before the intervention of Baduanjin. After the intervention, the angle and angular velocity of the knee joint during extension and flexion in standard group, overweight group and obesity group, and the angular acceleration of the knee joint during extension and flexion in standard group significantly increased. Baduanjin significantly increased the knee flexion force and moment in standard group, overweight group and obesity group, and increased the hip flexion force and moment as well as ankle metatarsal flexion force and moment in standard group. Compared with obesity group, the knee flexion force and moment in standard group were significantly different. The knee joint force and moment were positively correlated with BMI. Baduanjin significantly increased muscle strength of iliopsoas, gluteus medius, piriformis, gluteus maximus, rectus femoris, sartorius, tibialis anterior, biceps femoris longhead, flexor digitorum longus, flexor pollicis longus, musculus peroneus longus and musculus peroneus brevis. Baduanjin significantly increased the integrated electromyogram (iEMG） of rectus femoris, tibialis anterior, biceps femoris. Conclusions Baduanjin can improve muscle strength and neuromuscular control ability of young people. The larger the BMI, the greater the flexion force and moment of the knee joint.

太极拳； AnyBody仿真； 下肢； 动力学； 运动学

ObjectiveTo evaluate the efficacy and safety of agomelatin and selective serotonin reuptake inhibitors （SSRIs） in the treatment of depressive disorder via network Meta-analysis. MethodsThe literature databases such as China National Knowledge Network （CNKI）， Wanfang Data Knowledge Service Platform， VIP Database for Chinese Technical Periodical （VIP）， Chinese Biomedical Literature Database （CBM）， PubMed， Embase and Cochrane Library were searched from the inception to November 2021. Based on the preset inclusion and exclusion criteria， literature screening， quality assessment of methodology and data extraction were conducted by two researchers separately， then statistical analysis was carried out using ADDIS software. ResultsA total of 7 256 patients with depressive disorder in 22 randomized controlled trials were included. According to the consistency assessed in Bayesian network Meta-analysis and the estimation of the probability of being the best treatment， escitalopram （P=0.63） ranked first for response rate and paroxetine （P=0.31） was associated with the best ranking for cure rate in terms of the effectiveness， meantime， paroxetine （P=0.44） had the highest adverse events risk and sertraline （P=0.74） had the highest study drop-outs proportion in terms of safety. ConclusionEscitalopram and paroxetine may be superior to sertraline， agomelatine， citalopram and fluoxetine in the treatment of depressive disorder， furthermore， paroxetine and sertraline demonstrate poor safety profiles.

ObjectiveTo investigate the influence of interference tasks and age on gait characteristics and task costs of children aged three to eight years. MethodsFrom April to August, 2021, 200 children from a kindergarten and primary school in Cangzhou, Hebei were enrolled to collect gait spatiotemporal parameters and kinematics data with infrared motion capture system; ground reaction force was collected with the Kistler force platform and simulated with Anybody 7.0, as walking naturally (standard gait), answering question (cognitive gait) and crossing obstacle (obstacle-crossing gait). ResultsA total of 182 children finished the test. The main effect of task was significant on spatiotemporal parameters (F > 5.167, P < 0.01), as well as age (F > 2.321, P < 0.05), except on stride width and speed; while the interaction effect of task and age was significant on double stance phase, single stance phase and step length (F > 3.040, P < 0.01). The main effect of task was significant on kinematics data (F > 83.019, P < 0.001), as well as age (F > 2.359, P < 0.05), except on range of motion of knee and maximum angular velocity of ankle; while the interaction effect of task and age was significant (F > 2.066, P < 0.05), except on range of motion of hip. The main effect of task and age was significant on kinetic parameters (F > 4.032, P < 0.05); while the interaction effect of task and age was significant (F > 2.189, P < 0.05), except on the strength of medial soleus, lateral gastrocnemius, medial gastrocnemius and tibialis anterior. The coefficient of variation was the most for cognitive gait, and then for the obstacle-crossing gait and standard gait. The main effect of task and age was significant on the cost of task for stride length and speed (F > 3.368, P < 0.01), while the interaction effect was not significant. ConclusionGait of early childhood is influenced by interference tasks and age. Under interference tasks, gait cycle increases, while single stance phase, stride length, frequency and speed decrease; and task costs increase, and overall gait stability decrease. Cognitive tasks impact on gait greater than obstacle crossing, which may be due to the higher costs of tasks. In terms of age, gait exhibits a non-linear age trend.

Objective The single leg support tree pose and Yan balace of yoga was selected to compare the biomechanical characteristics of lower limb joint angles, joint force and muscle strength for professional and non-professional yoga practitioners, so as to provide theoretical basis for scientific fitness of yoga beginners and exercise prescription determined by rehabilitation physicians. Methods BTS infrared motion capture system was used to collect kinematic data, Kistler forceplate was used to collect dynamic data, AnyBody 7.0 simulation software was used to calculate the joint force and muscle strength, and Biodex balance system was used to evaluate the stability of single support. Results In both tree pose and Yan balance, the muscle strength of lower limbs in professional group was greater than that in non-professional group. Support leg of tree pose: the muscle strength of the tibialis anterior, gluteus medius posterior, iliac medialis and sartorius in professional group was greater than that in non-professional group (P<0.05). Balance leg of tree pose: the muscle strength of the biceps femoris breve, sartorius, piriformis, gemellus inferior, iliac lateralis, iliac medialis, gluteus maximus inferior, obturator internus in professional group was greater than that in non-professional group (P<0.05). Support leg of Yan balance: the muscle strength of tibialis anterior, extensor digitorum longus, extensor hallucis longus, obturator internus, gluteus maximus superior in the professional group was greater than the non-professional group (P<0.05). Balance leg of Yan balance: The muscle strength of extensor hallucis longus in professional group was greater than that in non-professional group (P<0.05). The scores of front-rear, overall of PS-BI, overall, front-rear, left-right of athlete single leg (ASL) in professional group were significantly lower than those in non-professional group (P<0.05). Conclusions The joint angle will affect the degree of muscle exercise in lower limbs. During yoga practice, attention should be paid to the standard of postures and the exercise of corresponding muscles. In tree pose, the muscle strength of both legs in non-professional group was significantly different from that in professional group, and the difference in Yan balance was more obvious on the supporting leg. Yoga practice can improve the stability of single leg support in multiple directions.