By the method of system identification, in this paper the mathematical models of the temperature control on fertility of the hasmaphysalis longicornis is established, and some predictions and estimations are given. The differences' between, the models and other models in the siminlar area are that,the models are coatinuous and differantiable other than segmented and undifferentiable

Objective To explore the elimination effect of radix Pulsatillae extracts on R plasmid of pseudomonas aeruginosa.Methods R plasmids of pseudomonas aeruginosa were isolated from the sputum of elderly patients suffering from respiratory infection,and the elimination test of radix Pulsatillae extracts on these plasmids was performed in vitro and in vivo.Results Being treated with the extracts for 24,48 and 72 h in vitro,the elimination rate of R plasmids was 0,1.8 %and 3.4 %respectively,while in SDS(sodium dode canesulphonate)control group the elimination rate was 0,0.8 %and 1.6 %respectively.Being treated with the extracts for 24,48 and 72 h in vivo,the elimination rate was 0,2 %and 12.8 %respectively,and 0 in the control group.Conclusions Both in vitro and in vivo,radix Pulsatillae extracts have an elimination effect on R plasmid of pseudomonas aeruginosa,and the effect in vivo is stronger than that in vitro.

Objective To carry on the theoretical research of nursing outcomes and indicators of hospitalized patients with coronary heart diease (CHD).Methods Self-designed questionnaire survey was conducted according to the Nursing Outcomes Classification,and then using it among 14 cardiology nursing experts.Results After experts questionnaire,determined 13 nursing outcomes,63 major indicators and 38 secondary indicators,while eliminated 9 indicators.Conclusion Formation of nursing outcomes and indicators of hospitalized patients with CHD is practical,professional,systemic and simplicity,it can be used as a tool to guide the clinical nursing practice.

Objective:To study the regulation effect of water extraction from Ganoderma Lucidum (Lyess. ex Fr) karst (GL W) on human immune cells and cytokine. Methods: With the technologies of MTT and FACS, the proliferation of T lymphocyte subgroup and IL 6 production in human peripheral blood lymphocytes (PBL) were studied. Results: 1. In the absence of mitogin, GL W could induce not only the inactive lymphocyte but also the PHA activited lymphocyte to proliferate in the similar concentration. The proliferation in the later was more obvious ( P

Cobra venom secretory phospholipase A_2 (sPLA_2) is an important component of cobra venom which has a variety of biological activities. Recent studies are mainly focusing on each pharmacological active component of venom, SPLA_2 is one of them. This review summarized the structure, purification and biological activities of cobra venom sPLA_2 with emphasizing its diverse pharmacological effects and toxicity. In addition, some mechanisms of actions of sPLA_2 and possible applications of sPLA_2 were also discussed.

Objective We sought to establish the standard operation procedures in department of cardiology.Methods According to the method of constructing standard operation procedures,the standard operation procedures for the department of cardiology was constructed through induction and consulting literature materials.Results 16 standard operation procedures for the department of cardiology were developed,including 8 SOP of common diseases to rescue,2 SOP of common operation in perioperative period,4 SOP for common instruments,2 SOP for common drugs usage.Conclusions Construction of standard operation procedures in department of cardiology can provide the basis for clinical nursing work,it can also supply methodological reference to build the standard operation procedures in other departments.

Aim To study effect of the extract of Fagopynum cymosum(Fr4) on the apoptosis and telomeraseactivity in human leukemia HL-60 cells.Methods HL-60 cells were treated with Fr4 and inhibition of proliferation was measured with MTT assay.Cell morphology before and after the treatment was examined with light and electron microscopy.FILC-Annexin-V and PI double staining was used to detect cell membrane-mediated apoptosis by FCM.DNA fragmentations were analyzed with DNA gel electrophoresis.Furthermore,telomerase activity was determined with PCR-ELISA-based telomeric repeat amplification(TRAP).Results The proliferation of HL-60 cells was inhibited by Fr4 with an IC_(50) of 115 mg?L~(-1).Typical morphology and biochemical feature of apoptosis was observed with Fr4 60～240 mg?L~(-1).The percentages of early apoptosis cells were increased in a dose-dependent manner.Telomerase activity was decreased in a dose-dependent manner during the process of apoptosis.Conclusion Fr4 induced apoptosis in HL-60 cells.Down-regulation of the telomerase activity in HL-60 cells might be contributed to Fr4-induced apoptosis.

Adenovirus Infections, Human ; epidemiology ; virology ; Adenoviruses, Human ; isolation & purification ; Caliciviridae Infections ; epidemiology ; virology ; Child, Preschool ; China ; epidemiology ; Diarrhea ; epidemiology ; virology ; Diarrhea, Infantile ; epidemiology ; virology ; Enzyme-Linked Immunosorbent Assay ; Feces ; virology ; Female ; Gastroenteritis ; epidemiology ; virology ; Humans ; Incidence ; Infant ; Male ; Norwalk virus ; isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction ; Rotavirus ; isolation & purification ; Rotavirus Infections ; epidemiology ; virology

Objective To study the application effect of discharge planning combined with continuous nursing in patients with chronic obstructive pulmonary disease (COPD).Methods From May 2014 to April 2015,92 cases of COPD patients in our hospital were selected as the research object,and they were divided into the observation group and the control group according to random number table,with 46 cases in each group. Patients in the control group received continuous nursing,while patients in the observation group were given discharge planning guidance on the basis of continuous nursing. The level of self-efficacy,anxiety, depression,and the treatment compliance of patients before and after intervention were compared between two groups.Results After intervention,the self-efficacy scores of safe behavior,environment and temperature, physical activity,emotional fluctuations,and dyspnea management in the observation group [(3.45±0.15), (2.96±0.24),(2.92±0.26),(2.97±0.24),(2.91±0.23)] were significantly higher than those in the control group [(3.15±0.17),(2.72±0.17),(2.81±0.18),(2.76±0.17),(2.83±0.14)] (P<0.05). The scores of self-rating depression scale (SDS) and self-rating anxiety scale (SAS) in the observation group were significantly lower than the control group [(21.45±2.02),(31.21±3.45) VS (35.43±2.54),(44.46±4.21)] (P<0.05). The treatment compliance in the observation group was significantly better than that in the control group (P<0.05). Conclusions For patients with COPD,discharge planning combined with continuous nursing can obviously improve patient's level of self-efficacy,anxiety,depression,and improve patients' treatment compliance to a certain extent. The effect is good with strong feasibility.

The aim of this study is to investigate the effects of electrical stimulation ES) on the induction of rat bone marrow mesenchymal stem cells (rBMSCs) to differentiate into cardiomyocyte-like cells in vitro. The third or fourth-generation of the rBMSCs was randomly divided into three groups, i. e. ES group, 5-Azacytidine (5-Aza) group, and control group. Those in the ES group with complete medium were exposed to 1,2,4 and 6V, 2Hz, 5ms ES for 2h everyday,lasting for 10d. Those in the 5-Aza group were induced by 10 micromol/L 5-Aza for 24h, then the medium was changed to complete medium without 5-Aza. Those in the control group were only cultured with complete medium. The growth status and morphological features of rBMSCs were observed by inverted phase microscope. The mRNA expressions of GATA4, a-actin, ACTN2 and TNNT2 were determined by Real-time fluorescent quantification PCR, and the protein expression of TNNT2 was detected with immunofluorescence staining. The results showed that the mRNA expression level of the GATA4, a-actin, ACTN2 and TNNT2 and the protein expression level of the TNNT2 were significantly higher in the ES group and 5-Aza group, compared to those in the control group(P<0. 05). It suggested that ES could induce rBMSCs differentiation into cardiomyocyte-like cells in vitro.
Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Electric Stimulation ; Female ; Male ; Mesenchymal Stromal Cells ; cytology ; Myocytes, Cardiac ; cytology ; Rats ; Rats, Sprague-Dawley