Objective To investigate the surgical techniques and clinical effect of laparoscopic improved myomectomy. Methods After the surgical techniques and instruments were improved, laparoscopic myomectomies were performed in 91 patients with uterine myoma. The maximum diameter of myoma was (60 4?11 9) mm (range, 25 mm～110 mm), and in 35 cases the myomas were ≥7 0 cm and in 56 cases 0 05). Conclusions After the surgical techniques and instruments were improved, the operation time and the blood loss can be reduced, and better clinical effect obtained, even in patients with somewhat larger uterine myoma.

Objective To investigate the surgical indications and techniques of laparoscopic operations for benign ovarian cysts. Methods A total of 112 patients underwent laparoscopic operations of cyst decollement, ovariotomy and adnexectomy. Results There were 104 cases (92 9%) of decollement, 5 cases (4 5%) of adnexectomy and 3 cases (2 7%) of ovariotomy.The operation time was (61 9?23 7) min, the intraoperative blood loss (37 0?40 7) ml, the postoperative hospital stay (6 8?2 4) days, the postoperative peak temperature of patients (37 8?0 5)℃, the duration of postoperative temperature beyond 37 5℃ (2 0?1 1) days and the time to first passing flatus (1 9?0 5) days. Pathological diagnoses showed 54 cases (48 2%) of ovary endometrial cyst, 30 (26 8%) of benign teratoma, 16 (14 3%) of mesonephroma, 8 (7 1%) of corpus luteum cyst, 3 (2 7%) of serous tumor and 1 (0 9%) fibroma. Conclusions Laparoscopic operations for benign ovarian cysts have advantages of minimally invasive surgery. However, indications must be strictly followed before operations, in the view of avoiding meeting malignant tumors in operations.

Objective To evaluate the middle-term effect of laparoscopic uterine artery occlusion combined with ascendant myomectomy (LUAO-M) for multiple uterine myomas. Methods The uterine artery was isolated and occluded with Kleppinger bipolar forceps (Sabre 2400,ASPEN LABS USA) or PK forceps (Gyrus Medical Limited Inc UK) under a laparoscope. Then dissection was performed on the surface of pseudo capsule with Kleppinger unipolar needle (Sabre 2400,ASPEN LABS USA) or PK needle (Gyrus Medical Limited Inc UK),and the target myoma was stripped out of the tumor bed with the Separate-Scoop device. Afterwards,repair of the incision was carried out in one layer with interrupted single stitch by Absorbable VICRYL suture (Johnson VICRYL ETHICON USA). Results The mean operation time was (102?36) min and mean blood loss was (88.7?58.4) ml. The mean hospital stay after the operation was (7.9?0.2) d,and febrile morbidity was 5.1% (5/98). Complications included two cases of subcutaneous emphysema and one case of ileus;no other severe complications occurred. Of the patients,98 cases were followed-up for 21 to 52 months (mean,36.3 months),during the period they were visited by a mean of 3.6 times,which showed a correction rate of menstruation abnormality of 95.9% (4/98),rate of uterine volume reduction of 57.7%,and rate of recurrent myoma of 3.1% (3/98). Conclusion LUAO-M shows a good clinical outcome and middle-term effect for multiple uterine myomas.

Objective To investigate the efficacy of integrated traditional Chinese medicine (TCM) with Western medicine in treatment of diarrhea type irritable bowel syndrome (IBS-D) and its effect on serum inflammatory cytokine levels.Methods One hundred and sixty four IBS-D patients treated in Guangfu Hospital from July 2013 to August 2015 were randomly divided into study group and control group with 82 cases in each group.All patients received oral Saccharomyces boulardii 1.0 b.i.d, while patients in study group received additional Shuganjianpi decoction b.i.d for 4 weeks.The clinical efficacy was observed, serum IL-10, IFN-γ and TNF-α levels were measured in 2 groups.Results After treatment, the total score of clinical symptoms in study group was lower than that of control group [(5.71±1.41) vs.(11.70±2.88) points,t=16.707, P<0.01].Serum levels of IFN-γ, TNF-α in study group decreased significantly after treatment [IFN-γ (2.88±1.38) ng/L vs.(1.00±0.44) ng/L, t=11.609, P<0.01;TNF-α (41.26±5.29) ng/L vs.(24.13±3.27) ng/L,t=24.636, P<0.01], IL-10 significantly increased [(142.23±21.58) ng/L vs.(170.23±33.45) ng/L,t=6.291,P<0.01].The overall effective rate of study group was higher than that of control group, [87.50% (70/80) vs.68.75% (55/80), x2=8.228, P<0.01].After treatment, the quality of life scores in both groups were improved;but the improvement of diet, spirit, mood and sleep scores in study group were better than those in control group [(240±69) vs.(193±60), t=4.579, (316±74) vs.(230 ± 69), t=7.603, (297±62) vs.(228±59), t=7.211;(284±62) vs.(230±54), t=5.874, all P<0.01].Conclusion The efficacy of integrated traditional Chinese medicine with Western medicine in treatment of IBS-D is significantly better than that of Western medicine alone, which may be associated with its regulatory effect on the serum inflammatory cytokine levels.

Objective:To explore the reliability and validity of Social Skills Inventory(SSI)applied in Chinese college students.Methods:A sample of 542 college students was administered SSI.Results:The Cronbach's ? coefficient of the SSI was 0.81;The test-retest reliability coefficient was 0.75;The Spearman-Brown's split-reliability of SSI was 0.84;The correlation coefficients of the six factors with the total score were 0.305～0.802;The indexes of confirmatory factor analysis were as follows:?2/df(2.2),GFI(0.888),CFI(0.903),IFI(0.916),RMSEA(0.076).Conclusion:SSI showed pretty good validity and reliability applied to Chinese college students.

AIM: To observe the protective effect of Qizhu Oral Liquid on radiation injury in mice. METHODS: The model was made in mice by X-ray radiation. The white blood cells(WBC), bone marrow nucleated cells (BMNC) were measured. The weight of thymus and spleen were measured. RESULTS: Qizhu Oral Liquid could increase WBC and BMNC remarkably. It could increase the weight of thymus and spleen of mice signifcantly. CONCLUSION: Qizhu Oral Liquid is effective in protecting the X-radiation injury mice.

ObjectiveTo evaluate the effects of Ang Ⅱ on apoptosis of podocytes and explore the signaling pathwayof nephrin in preventingAng Ⅱ-inducedpodocyte apoptosis.MethodsDifferentiated mouse podocytes were exposed to Ang Ⅱ at different concentrations for 18 h or at 10-8 mol/L for variable incubation times.Undifferentiated mouse pedocytes were transfected using lipofectamine 2000 with the pcDNA3.1-mNPHS1 plasmid and stably transfected cell lines were generated with G418 selection.In separated experiments,untransfected mouse podocytes (MPC) and stably transfected podocytes with pcDNA3.1-neo and PcDNA3.1-mNPHS1 were exposed toAng Ⅱ(10-8 mol/L) or LY294002(a selective Akt inhibitor,50 μmol/L) for indicated times.Apoptosis was evaluated by flow cytometry.The expression of nephrin was assessed by quantitative real-time PCR,immunofluorescence and Western blotting.The phosphorylation level of Akt was determined by Westem blotting.Results(1) AngⅡ promoted podocyte apoptosis in a dose-and time-dependentmanner. PretreatmentwithlosartansignificantlypreventedAngⅡ -induced apoptosis. (2) Nephfin mRNA and protein were obviously decreased in podocytes exposed to 10-8 mol/L Ang Ⅱ for at least 12 h than those in vehicle-treated cells (P＜0.05).(3) Ang Ⅱ exposure for more than 15 min inhibited the phosphorylation of AKT in MPC,which was dramatically reversed by pcDNA3.1-mNPHS1 transfection,but not by pcDNA3.1-neo transfection. (4) Podocyte apoptosis was promoted byLY294002. Conversely,Ang Ⅱ-induced podocyteapoptosis was significantly alleviated by pcDNA3.1-mNPHS1 transfection.ConclusionAng Ⅱinduces mouse podocyte apoptosis which is suppressed by overexpression of nephrin through PI3K-Akt signaling pathway.

In recent years,the relationship between fatty acid metabolism and tumor has become a hot topic and widely recognized.In tumor cell,fatty acid metabolism is important to its growth and metastasis.About synthesis,fatty acid can participate in the synthesis of phospholipids on the membrane of cancer cells and take part in important signal transduction pathways,such as PI3K-AKT-mTOR;in fatty acid catabolism,tumor cells can produce ATP to maintain its growth by β-oxidation;what's more,it could be kept redox homeostasis by producing nicotinamide adenine dinucleotide phosphate hydrogen (NADPH).So,anabolism and catabolism of fatty acid may promote occurrence and development of tumors,but the mechanisms are unclear.This review highlights the effects of fatty acid metabolism on the occurrence,development and metastasis of tumors.

The tumor-associated neutrophils (TANs) are thought to be important and complicated character in tumor microenvironment.TANs play dual roles of anti-tumor or tumor-promoting in tumor microenvironment under different factors.TANs can not only play the anti-tumor effect by killing tumor cells directly and promoting immune response,but also can act as tumor promotors by promoting tumor angiogenesis and mediating tumor invasion and metastasis.Recent researches find that TANs are closely related to pre-metastatic niche (PMN),which can promote the construction and maintenance of PMN by means of changing the content of cell factors like interferon-γ.

BACKGROUNDTo observe the suppressive effects of exogenous p27 gene on human lung cancer cell line A549.

METHODSAn adenovirus expression vector (pAd CMV-p27) containing 570 bp human full-length p27 cDNA was transfected into human lung cancer cell line A549. Expression of exogenous p27 gene was detected by dot-blot hybridization and laser co-focal system. MTT was adopted to measure the effects of exogenous p27 gene on cell cycle progression and cell features of the infected A549.

RESULTSThe mRNA and protein expression level of p27 was remarkably increased after transfecting with exogenous p27 gene. The apoptosis of infected A549 occurred and the progression of cell cycle was arrested in G1 phase.

CONCLUSIONSp27(kipl) gene transfer may play a therapeutic role in the treatment of lung cancer.