Objective To observe safety and effectiveness of Dezocine in postoperative analgesia of pediatric tonsillectomy and adenoidectomy by the continuous intravenous infusion of different doses,and to explore appropriate dosage of Dezocine for pediatric postoperative analgesia.Methods Elective tonsillectomy and adenoidectomy require-ments of postoperative analgesia in children 120 cases,they were randomly divided into A,B,C,D groups of 30 cases by using number table.After surgery patient -controlled intravenous analgesia(PCIA)two days,A,B,C groups were given Dezocine 0.2,0.3,0.4mg·kg -1 ·d -1 continuous intravenous infusion,D group received Sufentanil 1μg· kg -1 ·d -1 continuous intravenous infusion.Observed and recorded pain scores and sedation scores for postoperative 6h,12h,24h and 48h;recorded adverse reactions,pressing the number and effective pressing times of children within 48h after surgery.;recorded overall evaluation of postoperative analgesia of children and parents.Results A group at each time point pain score was significantly higher than B,C,D three groups,the difference was statistically significant (tAB =12.003,PAB =0.049;tAC =7.542,PAC =0.036;tAD =40.990,PAD =0.009);A,B,C three groups at each time point sedation score was significantly lower than D,the difference was statistically significant(tDA =3.894,PDA =0.030;tDB =5.020,PDB =0.039;tDC =36.103,PDC =0.016);D group of adverse reactions were significantly higher than A,B,C three groups,the difference was statistically significant(χ2DA =20.738,PDA =0.000,χ2DB =4.35,PDB =0.029,χ2DC =5.98,PDC =0.025);A group within 48h postoperative analgesia press times and the number of valid pressing were significantly higher than B,C,D three groups,the difference was statistically significant(tAB =3.003, PAB =0.036;tAC =5.720,PAC =0.031;tAD =37.157,PAD =0.015);B,C two groups of children and parents to evaluate postoperative analgesia overall satisfaction rates were 90.0%,93.3%,significantly higher than A 63.3%,D 60.0%,and the difference statistically significant (χ2AB =5.310,PAB =0.031;χ2DC =19.640,PDC =0.000). Conclusion Dezocine 0.3mg·kg -1 ·d -1 continuous intravenous infusion for pediatric tonsillectomy and adenoid-ectomy is safe and effective in postoperative analgesia and fewer adverse reactions,and should be promoted.

Objective To analyze the value of carbon nanoparticles in the cleaning of thyroid cancer lymph node.Methods 80 patients with thyroid cancer from March 2012 to March 2013 were included in this study.Based on the sequence of enrollment,they were randomly divided into two groups:the therapy group (n=40)were received Nano carbon lymphatic tracer before basic operative treatment and the control group (n=40)were directly received the same basic operative treatment.The liver function parameters in two groups were observed and compared statistically. Results The operating time,operative blood loss and postoperative drainage volume had no statistically significant difference between two groups.The incidence of postoperative hypocalcemia in experimental group was lower than that in blank control group(P<0.05 ).The parathyroid hormone level in experimental group on the first postoperative day was higher than that in blank control group(P<0.05 ).The number of resected lymph nodes in experimental group was more than that in blank control group(P<0.01).Conclusion Nano carbon lymphatic tracer technique can increase the cleaning number of lymph node of thyroid cancer patients in central region,and realize the accurating tumor stage.

In order to investigate the possibility of analysis of single strand DNA by using DNA biosensor, we developed a quartz crystal DNA biosensor and studied its performances. The single strand DNA probe was immobilized on the surface of gold filled quartz crystal by biotin immunoreaction. The DNA biosensors were used to detect the complete complementary and single base mismatch complementary single strand DNA in solution. The DNA biosensors were regenerated with 0 1mol/L HCl after hybridization. The hybridization sensitivity of quartz crystal DNA biosensor is 10ng/ml. In the range of 5～40ng/ml, there was a linear correlation between responding signals and concentrations of complementary single strand DNA with a high specificity. The DNA biosensor could be repeatedly used over 5 times by using 0 1mol/L HCl for regeneration.

Objective To form Hepatitis B electrochemical immunosensor for the clinic detection. Methods Using the gold electrode as the fundus,the thionine and the HBV horseradish peroxidase(HRP) antibody were electrostaticly bounded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immunosensor. Cyclic voltammetry and linear scan voltammetry were used to investigate the chemical characteristics of the immunosensor. Results The linearity of Hepatitis B in the range of 1:30～1:200 with the correlation coefficient of 0. 994 was obtained. Conclusion The Hepatitis B electrochemical immunosensor formed by this way can be used to the clinic detection.

Objective To develop a portable analyzer which can rapidly detect antigen of type B hepatitis,antibody of HIV and H5N1 in blood samples.Methods The micro-processor of STC89C52 was adopted as the controlling core in the system.Based on an embedded technology,the analyzer can rapidly detect the antigen of type B hepatitis,antibody of HIV and H5N1 by processing the synchronic frequency of quartz crystal micro-array and by sensitivity of quality on the surface of quartz crystal with special identification between antibody and antigen.Results The system has the advantages of high sensitivity,better sample-specific characteristics,convenient portability and multi-parameter simultaneous detection.Conclusion The piezoelectric analyzer exceeds the traditional reagent method and serves for institution of scientific research and hospital widely.

Objective To construct a kind of novel electrochemical glucose biosensor for clinical detection.Methods Multiwall carbon tube(MWCNTs),NanoAu and glucose oxidase(GOD) were immobilized on the Au electrode by monolayer self-assemble.Cyclic voltammetry and linear scan voltammetry were used to investigate the assembling process and the chemical characteristics of the glucose biosensor.Results The linearity of glucose in the range of 10～1 400 mg/L with the correlation coefficient of R2=0.990 9 was obtained by linear scan voltammetry and the detection limited reached 0.000 1 mg/L.The four criterion samples were measured with 3 different biosensors in same conditions and the RSD

Nano-materials are immensely applied in piezoelectric, electrochemical, optical, magnetic biosensors based on clearly unique nature of it. The important role of nano-materials in biosensors is expounded and the latest application of novel functional nano -materials in biosensor is introduced emphatically, such as nanoparticles, nanotubes, nanowires, quantum dots and nanofibers. The researches make clear that nano-materials can provide momentous value in enhancing performance of biosensors, such as sensitivity, detection range, and repetitiveness of biosensors, as immobilization materials or carriers.

The cooperation of NO air-feed system and respirator, in which the integrative and separate modes are involved, is introduced in this paper. Its disadvantages and development trend are also discussed on the bases of the constitution of the mixed gas containing NO and O2/air, the synchronous working of NO air-feed system and respirator and the inspection of NO/NO2 gas.

SPR biosensor can inspect a lot of biochemical indexes exactly,sensitively,fast and simply.It also can inspect reciprocity of inter-molecule in real time.In 20 years,it has become a popular photoelectric technique in clinical examination.This article introduces the basic principle,structure and application of SPR biosensor in clinical examination.

Objective To investigate the resistance rate of Pseudomonas aeruginosa to several commonly used antibiotics,as well as the prevalence status of three species of integrons in multi-drug resistant Pseudomonas aeruginosa from 2005 to 2006 in our hospital,and to identify the genetic structure of class Ⅰ integron in in our hospital.Methods Drug resistance slip method was applied to detecting the resistant rates of 100 isolates of Pseudomonas aeruginosa to ciprofloxacin,cefotaxime,gentamicin and imipenem.Boiling method was adopted to abstract DNA template of 100 isolates of Pseudomonas aeruginosa,and PCR assay adopted to identify the prevalence rate of three species of integrons and class Ⅰ integron structure.Results The resistant rates of 100 isolates of Pseudomonas aeruginosa to ciprofloxacin,cefotaxime,gentamicin,cefoxitin,tobramycin and imipenem were 32%,45%,82%,37%,39% and 43% respectively.The prevalence rates of three species of integrons was as follows:class Ⅰ 53%,Ⅱ 21% and Ⅲ 5%.Sequence analysis displayed there existed box gene dfrA17 and aadA2 in variable region of class Ⅰ integron.Conclusion The resistant rates of Pseudomonas aeruginosa to four drugs are elevated,especially to gentamycin.Multi-drug resistance may be partly due to prevalence of class Ⅰ integron.