Radiofrequency ablation(RFA) is a local and minimally-invasive therapy.In recent years,RFA has been increasingly practiced in the clinical treatment of bone tumors.RFA is very safe and effective in relieving pain caused by inoperable metastatic bone lesions and in reinforcing the ablated vertebra when combined with bone cement injection,which is helpful in preventing pathologic fracture.RFA offers an ideal and effective alternative for patients with inoperable metastatic bone tumors.

Objective To observe the repairing effects of myoblasts from mesenchymal stem cells induced by MyoD transfection on muscle injury, and to explore its mechanism. Methods One hundred and sixty male SCID mice were randomly assigned into 4 groups [normal group, control group with injury, implantation with mesenchymal stem cells (MSCs) group, and implantation with myoblasts group]. MSCs were transfected by pIRES2-EGFP-MyoD and differentiated into myoblasts. Myoblasts and MSCs were injected respectively into the muscular tissue injuried by cardiotoxin. The repairing effect in injuried muscles, which were injected with myoblasts and MSCs, was observed 1, 2, 4 and 6 weeks after the injection. Results The muscular tissue injury model was successfully reproduced. Both MSCs and myoblasts showed obvious repairing effects on the injured muscular tissue, and the strength of muscular tissue in myoblasts group was stronger than that in MSCs group. Western blot assay showed that MyoD expression in myoblasts group was much higher than that in both MSCs and control groups, the expressions of JNK1 and ERK2 were up-regulated in myoblasts group, and the p38 expression was down regulated significantly in the 1st week, but no significant difference was found when compared with those of the normal group at the 6th week. Conclusion Myoblasts transdifferentiated from MSCs induced by MyoD can repair the injuried muscular tissue effectively. JNK1, p38 and ERK2 play important roles in the repairing process.

Objective To study the effects of estrogen on the cultured osteoclasts in vitro. Methods The acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities were measured kinetically and the introcellular hydrogen ions were calculated with the confocal laser scanning microscopy(CLSM). The area and number of the resorption pits were determined with the Leica Quantimet 500 system. Bone resorption pits were observed with scanning electronicmicroscope. Results With the estrogen concentration increased, the ACP and TRAP activities decreased from (1 69?0 13) U/L and (1 60?0 14) U/L to (1 16?0 31) U/L and (0 93?0 34) U/L, respectively (experiment group vs control group, P

Objective To evaluate the clinical and X-ray features of bone lesion in early congenital syphilis. Methods Between 1997 and 2005, 48 cases of early congenital syphilis were diagnosed. Among those 48 cases, there were 16 cases with bone lesions detected. The clinical and radiographic features of these 16 cases of bone lesion in early congenital syphilis were retrospectively analyzed. There were 10 males and 6 females with an age between 25-57 d. All patients were confirmed with serology and radiographs of limbs. They were given Penicilin G of 15-20 WU?kg-1?d-1, 3 times a day in a total of 14 days for one course of treatment. Results Rapid plasma reagent test(RPR) of all the cases was positive and they were 3 cases with 1∶128, 2 cases with 1∶64, 6 cases with 1∶32, 3 cases with 1∶16 and 2 cases with 1∶8. All the cases were positive for TPPA. 30 parents of 16 cases were tested for RPR and TPPA. The results showed that 16 mothers were positive for RPR, 13 fathers were positive for RPR. All the parents were positive for TPPA, and negative for HIV test. The main clinical features included active disorder, swelling of involved limbs, malnutrition, light body weight and multi-organ functional lesion. The main X-ray features were metaphysitis, periostitis and osteomyelitis involving all of the long bones of the extremities. Widen of the junction between epiphysis and diaphysis was the characteristic features of early congenital syphilis. After treatment, RPR became negative and sclerotic lesion was recovered and only remain periosteum proliferation. Hutchinson teeth were seen in some patients. Conclusion Bone lesions in early congenital syphilis has its characteristic clinical and radiographic features. The bone lesion could be cured after effective treatment.

Nowadays, lung cancer has become one of the most common malignant tumors. The animal models of lung cancer have played very important role in the research on the etiology, diagnosis and treatment of human lung cancers. According to the preparation method, the animal models of lung cancer can be divided into spontaneous, induced, transplanted and transgenic models. The transplanted animal models are most commonly used. Transplanted animal models can be classified into heterotopic transplantation model and orthotopic transplantation model, each has its own characteristics. This article aims to introduce the research progress of transplanted animal models of lung cancer in recent years, focusing on the establishment of animal model, the improvement of preparation of animal model, the application of imaging in vivo in evaluating the animal model, the use of transplanted animal model in the study of interventional therapy, etc.

Objective: To explore the influencing factors for depressive symptoms in adolescents in China, so as to provide insights into promoting mental health of adolescents. Methods: The 2020 follow-up survey data of China Family Panel Studies were collected, including demographic information, lifestyle, family factors and academic factors of adolescents aged 10-19 years. Depressive symptoms were evaluated using the 8-item Center for Epidemiological Studies Depression Scale. The influencing factors for depressive symptoms in adolescents were analyzed using a multivariable logistic regression model. Results: A total of 2 777 adolescents were analyzed, including 1 470 males (52.93%) and 1 307 females (47.07%). There were 1 186 adolescents (42.71%) from urban areas and 1 591 adolescents (57.29%) from rural areas, 106 smokers (3.82%), and 459 adolescents (16.53%) with depressive symptoms. Multivariable logistic regression analysis showed that academic stress (OR=1.268, 95%CI: 1.151-1.396), poor self-rated health (OR=1.255, 95%CI: 1.116-1.411), smoking (OR=1.901, 95%CI: 1.127-3.207), low trust in parents (OR=0.780, 95%CI: 0.729-0.835) and large family size (OR=1.095, 95%CI: 1.035-1.158) were associated with an increased risk of depressive symptoms in adolescents. Conclusion The influencing factors for depressive symptoms in adolescents were academic stress, self-rated health, smoking, trust in parents and family size.

Objective To summarize clinical diagnosis and microsurgical treatment method of cerebellopontine angle epidermoid cyst presenting with trigeminal neuralgia.Methods Thirty-seven cases of cerebellopontine angle epidermoid cyst presenting with trigeminal neuralgia treated by microneurosurgery procedure were analyzed retrospectively.Results Complete resection was achieved in 31 cases,and subtotal was achieved in 6 cases,with no operative mortality.Postoperatively,the symptom of trigeminal neuralgia disappeared in 28 cases,was eased obviously in 6 cases,and was eased in 3 cases.The symptom of dizziness in all 6 cases vanished,and hearing in 3 cases was improved.Three cases appeared cerebrospinal fluid leakage,and 4 cases appeared oral herpes.Symptom in 1 case aggravated after 6 months,and oral medicine was ineffective.The pain symptom disappeared after reoperation.Conclusions The suboccipital retrosigmoid approach microneurosurgery is the principal treatment method for cerebellopontine angle epidermoid cyst presenting with trigeminal neuralgia.Microsurgical technique combined with surgical skill is the guarantee for increasing total removal rate of tumors and decreasing complications.

A total of 35 patients with hallus valgus (56 feet) received treatment at the Department of Orthopedics, the Sixth People's Hospital, Shanghai Jiao Tong University between January 2004 and June 2005 and were recruited into this study. These patients underwent osteotomy of proximal first metatarsal and internal fixation with poly (L-lactic acid) (PLLA) absorbable pins. All patients were followed up for 4-22 months. Excellent outcomes were found in 27 patients (44 feet) and good results in 8 patients (12 feet). The mean healing time of surgical region was 6 weeks, nonunion or delayed union was not found. There were 3 cases suffering from complications post surgery. Two of them presented with peripheral wound red swelling, considering as a result of extraneous material-caused aseptic inflammatory reaction. This phenomenon would be regressed 2-4 weeks after hydropathic compress of ethanol gauzes and timely renewal of dressings. There was 1 case who suffered from protrution of absorbable pin 1 week after fixation and received reposition and plaster fixation. Bone displacement and nonunion were not found. This complication maybe results from excessively large bone pore due to shaking of electrodrill, which leads to loosening of absorbable pins.

AIM:To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules(Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.)by microbore LC.METHODS:Microbore liquid chromatography was applied.Microsil C_ 18 column(150 mm?1.0 mm)was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 ?L/min,and the detection wavelength was set at 220 nm.RESULTS:The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations(RSD)were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION:This method is simple,convenient and can be used for quality control.

Objective To express and detect the antigenicity of human laminin alpha4 LG3-4 module (hLN?4LG3-4) protein by gene engineering techniques. Methods The cDNA encoding hLN?4LG3-4 was amplified by RT-PCR from human placenta, then inserted into pMD-18T vector by T/A cloning and sequenced. Prokaryotic expression vector pET-28a-LG3-4 was constructed by recombinant DNA technique. The hLN?4LG3-4 fusion protein expressed in BL21(DE3)/pET system was identified by SDS-PAGE, purified by Ni-NTA resin, and assayed by Western blotting. Results The cDNA fragment of hLN?4LG3-4 was cloned successfully. While BL21 (DE3)/pET28a-LG3-4 bacterium was induced with IPTG, a new protein band with a relative molecular weight of 44 000 was shown on SDS-PAGE profile. hLN?4LG3-4 fusion protein of high purity (95%) was obtained and specific protein band was detected by Western blotting. Conclusion The hLN?4LG3-4 fusion protein was successfully expressed.