Imaging-guided radiofrequency ablation(RFA) is a safe and minimally-invasive interven-tional therapy with satisfactory short-term efficacy and less complications.Nowadays,RFA has been widely and successfully applied in the clinical practice for the treatment of hepatocellular carcinomas,lung cancers,adrenal malignancies,bone tumors and hypersplenism.For recent years,a combination of RFA with transcatheter arterial chemoembolization,chemical ablation or percutaneous vertebroplasty has become a new interventional therapeutic pattern for treating the tumors comprehensively,and it can be anticipated that by combining RFA with other therapeutic programs the effectiveness for tumors will be further improved.

Objective To study the effects of estrogen on the cultured osteoclasts in vitro. Methods The acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities were measured kinetically and the introcellular hydrogen ions were calculated with the confocal laser scanning microscopy(CLSM). The area and number of the resorption pits were determined with the Leica Quantimet 500 system. Bone resorption pits were observed with scanning electronicmicroscope. Results With the estrogen concentration increased, the ACP and TRAP activities decreased from (1 69?0 13) U/L and (1 60?0 14) U/L to (1 16?0 31) U/L and (0 93?0 34) U/L, respectively (experiment group vs control group, P

ObjectiveTo explore change trend of Cardiac Troponin Ⅰ (cTnI) and Heart Fatty Acid-binding Protein(H-FABP) in serum during the perioperation of valve replacement.MethodsForty patients with heumatoid valvular heart disease were selected for this study,and the patients were randomly divided into two groups.Blood samples were taken from center vein,and the serum levels of cTnI and H-FABP were determined.The change of the serum levels of these two markers at different time points was recorded and compared between two groups.ResultsThere were significant differences in the concentration of cTnl in the cold crystalloid cardioplegia group ( F between group =2744.397,P ＜0.01 ; F interaction =125.345,P ＜0.01 ).There were significant differences in the concentration of cTnI in the cold blood cardioplegia group ( F between group =1056.357,P ＜ 0.01 ; Finteraction=64.242,P ＜ 0.01 ).There were significant differences in the concentration of H - FABP in the cold crystalloid cardioplegia group ( F between group =1775.022,P ＜0.01; F interaction =34.297,P ＜0.01 ).There were significant differences in the concentration of H -FABP in the cold blood cardioplegia group ( F between group =3064.451,P ＜0.01; Finteraction=60.472,P ＜O.01 ).ConclusionsH-FABP is suitable for early diagnosis of myocardial injury because of its myocardial specificity.There are positive correlation between the peak of H-FABP and myocardial injury.There are effective and short period of window for prediction of myocardial injury caused by H-FABP.H-FABP may be served as a good myocardial injury diagnostic markers.

Radiofrequency ablation(RFA) is a local and minimally-invasive therapy.In recent years,RFA has been increasingly practiced in the clinical treatment of bone tumors.RFA is very safe and effective in relieving pain caused by inoperable metastatic bone lesions and in reinforcing the ablated vertebra when combined with bone cement injection,which is helpful in preventing pathologic fracture.RFA offers an ideal and effective alternative for patients with inoperable metastatic bone tumors.

The early functional changes of the heart after radiation, burn or combined radiation-burn injury were studied with an isolated working heart preparation of rats. The animals were randomized into the control group (C), the burn injury group (B) inflicted with 30% TB-SA full thickness burns from a 5 kw bromine-tungsten lamp, the radiation injury group (R) inflicted with total body irradiation of 6 Gy from a "Co source and the combined radiation-burn injury group (RB) receiving both of the injuries. No treatment was administered after injury. Left ventricular systolic pressure (LVSP). maximum of LV pressure development ( ? dp/ dtmax), heart rate (HR), cardiac output (CO), coronary flow (CF), aortic pressure (A.P) and ratio of dry/wet myocardial weight (Rw) of the perfused isolated heart were determined in the 1st, 3rd, 8th, 16th and 24th hour after injury. It was found that LVSP, ?dp/dtmax, CO and AP were decreased in RB especially in the 8th hour after injury ( P

Objective: To explore the influencing factors for depressive symptoms in adolescents in China, so as to provide insights into promoting mental health of adolescents. Methods: The 2020 follow-up survey data of China Family Panel Studies were collected, including demographic information, lifestyle, family factors and academic factors of adolescents aged 10-19 years. Depressive symptoms were evaluated using the 8-item Center for Epidemiological Studies Depression Scale. The influencing factors for depressive symptoms in adolescents were analyzed using a multivariable logistic regression model. Results: A total of 2 777 adolescents were analyzed, including 1 470 males (52.93%) and 1 307 females (47.07%). There were 1 186 adolescents (42.71%) from urban areas and 1 591 adolescents (57.29%) from rural areas, 106 smokers (3.82%), and 459 adolescents (16.53%) with depressive symptoms. Multivariable logistic regression analysis showed that academic stress (OR=1.268, 95%CI: 1.151-1.396), poor self-rated health (OR=1.255, 95%CI: 1.116-1.411), smoking (OR=1.901, 95%CI: 1.127-3.207), low trust in parents (OR=0.780, 95%CI: 0.729-0.835) and large family size (OR=1.095, 95%CI: 1.035-1.158) were associated with an increased risk of depressive symptoms in adolescents. Conclusion The influencing factors for depressive symptoms in adolescents were academic stress, self-rated health, smoking, trust in parents and family size.

Objective To explore the diagnostic value and optimal cut-off point of symptom index (SI) in gastroesophageal reflux-induced chronic cough (GERC).Methods The recordings of multichannel intraluminal esophageal impedance and pH monitoring were retrospectively analyzed in 118 patients with suspicious GERC.SI for all the refluxes,acid reflux and non-acid reflux was calculated respectively by analyzing the temporal association between detected reflux and cough recorded on diary card.Based on the favorable response to the anti-reflux therapy,the diagnostic value for GERC of SI was evaluated and compared with that of the symptom association probability (SAP).Results GERC was definitely determined in 100 patients (84.7％).When SI for all the refluxes was used for the diagnosis of GERC,the cut-off point of ≥45％ had the highest diagnostic efficacy,with the sensitivity of 56.0％,the specificity of 83.3％and Youden index of 0.393.SI for acid or non-acid reflux had the same optimal cut-off point of ≥30％ and presented with the similar efficacy in the diagnosis of acid or non-acid GERC.Compared with SAP of ≥75％,SIforall the refluxes of ≥45％ had a lower sensitivity (56.0％ vs 75.0％,x2 =7.988,P=0.005),a higher specificity (83.3％ vs 44.4％,x2 =5.900,P =0.015) and the comparable positive or negative predictive value in the diagnosis of GERC.The diagnostic accuracy for GERC was further improved when combining SI for all the refluxes with SAP.Conclusion SI for all the refluxes has a diagnostic value similar to SAP and its optimal cut-off point for GERC may be ≥45％.

Objective To investigate the effect of Bi-level positive airway pressure ventilation (BiPAP) combined with Seretide on quality of life of elder patients with moderate and severe chronic obstructive pulmonary disease (COPD) and type Ⅱ respiratory failure.Methods Eighty elderly patients with moderate to severe COPD and type Ⅱ respiratory failure were selected and randomly divided into observation group (n =40) and control group(n =40).The two groups were given conventional anti infection,oxygen inhalation,spasmolysis,expectorantand other conventional treatment,and the use of BiPAP ventilator assisted ventilation.Patients in observation group were administered the combined of Seretide and BiPAP treatment,and patients in control were received only BiPAP.Arterial blood gases before and after 7 d treatment,lung function and quality of life evaluation results (SQGR score) before and after 7 d,3 months and 6 months treatment,were measured.Results There were significant differences in terms of arterial blood gases,pulmonary function key indicators,SQGR score between two groups before and after treatment(P ＜ 0.01).PaCO2 was significantly after 7 d of treatment in observed group was (42.9 ± 7.9) mmHg,lower than that of the control group ((47.6 ± 8.0) mmHg; t =8.467,P ＜0.001).There was no significant difference in terms of forced expiratory volume in one second(FEV1) in both groups at 7 d,3 months,6 months after treatment (P ＞ 0.05).FEV1/forced vital capacity (FVC) in two group at 3 months,6 months after treatment were different compared to 7 d after treatment (observation group:(49.9 ±5.1)％ and (47.1 ±4.2)％,(50.2 ± 5.0)％ and(47.1 ±4.2)％ ;control group:(49.0 ± 5.4)％and (46.6 ± 5.9) ％,(49.8 ± 5.2) ％ and (46.6 ± 5.9) ％ ; P ＜ 0.05).SQGR score in observation group at six months after treatment were (40.8 ± 8.5),significantly lower than that of 7 d after treatment(45.9 ± 10.8),P ＜ 0.05),and significantly lower than the control group after 6 months of treatment ((40.8 ± 8.5) vs (46.0± ± 8.0),P ＜ 0.05).Conclusion Seretide combined with BiPAP treatment can significant improve lung function and the quality of life of patients with moderate and severe COPD and type Ⅱ respiratory failure.

OBJECTIVE To discuss the diagnosis and treatment of Burkholderia cepacia(BCE) pneumonia. METHODS The 31 cases of inpatients and outpatients in our hospital were analyzed from Jul 2005 to Dec 2007. RESULTS From them 11 cases were with community-acquired pneumonia and 20 cases with hospital-acquired pneumonia,the underlying diseases in 27 cases.Of these cases,19 cases were kidney transplant recipients,four cases with chronic obstructive pulmonary disease,seven cases with non-insulin dependent diabetes,three cases with cerebral vascular diseases,and two cases were with malignant tumors.All cases were diagnosed by bronchoscopy,bronchial lavage,and BCE positive in douche liquid.At last there were 24(77.4%) cases cured,2 cases discharged(6.5%) and 5 cases deceased(16.1%). CONCLUSIONS BCE is a multi-drug resistant bacteria.BCE pneumonia often appears severe.It appears non-specific clinical symptoms in early stage and higher mortality.It should be treated as soon as possible by using piperacillin /tazobactam-based combined anti-infection treatment.

AIM: To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules (Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.) by microbore LC.METHODS: Microbore liquid chromatography was applied.Microsil C_(18) column( 150 mm×1.0 mm) was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 μL/min,and the detection wavelength was set at 220 nm.RESULTS : The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations (RSD) were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION: This method is simple,convenient and can be used for quality control.