Objective To study the effects of resveratrol against toxicity induced by arsenic trioxide in rats.Methods Arsenic trioxide was given to SD rats intraperitoneally for 4 weeks.Resveratrol was injected intraperitoneally to the animals 4 h before arsenic trioxide challenge at dose of 30,60,or 120 mg?kg-1?d-1.Four weeks later,the contents of malondialdehyde(MDA),superoxide dismutase(SOD),nitric oxide(NO),glutathione peroxidase(GSH-Px) in serum,lung,spleen,liver and brain tissues were measured.Results Compared with the normal control rats,arsenic trioxide challenge resulted in increases of NO and MDA contents in serum,lung,spleen,liver and brain tissues,and decreases of GSH-Px and SOD levels in the rats received arsenic trioxide only.Resveratrol significantly inhibited these changes induced by arsenic trioxide in rat serum,lung,spleen,liver and brain tissues in a dose-dependent manner.Conclusion Resveratrol can protect antiperoxidase activity and inhibit peroxide productions.

Objective To study the growth and development as well as reproductive capacity of CL Long Evans rats. Methods Eighty CL Long Evans rats(80 days old)were bred in a barrier system. A monogamous mating system is used to reproduce 5 litters. Their main indexes of growth and reproduction were determined. The body weight gain of 60 second litter rats were analyzed from day 20 to 100. Results The number and weight of the fetus, the weaning time weight per cote and per litter in the second litter were the highest, and the fifth litter was the lowest. The growth and development curve was the same as that announced by Taconic. Conclusion Long Evans rats have been confirmed suitable for continual reproduction for 4 litters and the second litter is the best one for mating.

Purpose To investigate the prenatal ultrasonic features of fetal hypospadias and to compare the diagnostic accuracy.Materials and Methods The clinical data of 28 patients with suspected or confirmed hypospadias were retrospectively analyzed.The prenatal ultrasonic features,missed diagnosis and misdiagnosis of the patients were analyzed,and the diagnostic accuracy of different ultrasonic features for hypospadias was compared.Results In 28 cases,21 cases of hypospadias were confirmed by examination after birth or induced abortion with 2 cases of missed diagnosis by ultrasound,and 7 cases of non hypospadias were misdiagnosed by prenatal ultrasound.The diagnostic accuracy rate of 2D ultrasound was 67.9％ (19/28),and that of 3D ultrasound combined with 2D ultrasound was 92.9％ (13/14).The diagnostic accuracy rates of ultrasonic features such as abnormal urinary jet,abnormal urethra,tulip sign,abnormal penis tip,scrotum separation with or without penoscrotal transposition and short penis were 100.0％ (6/6),92.9％ (13/14),88.2％ (15/17),83.3％ (5/6),25.0％ (1/4) and 33.3％ (1/3),respectively.Conclusion The ultrasonic features including abnormal urinary jet,abnormal urethra,tulip sign and abnormal penis tip have great value for prenatal diagnosis of fetal hypospadias.3D ultrasound is more intuitive than 2D ultrasound and provides more details.Comprehensive analysis of all ultrasonic features is helpful for prenatal diagnosis.

Objective To evaluate the feasibility of negative pressure suction enteroscopy for intestinal examination.Methods The negative pressure suction enteroscopy was first applied to examine 20 detached small intestines from pigs to explore a suitable work pressure and to detect possible intestinal damages.The procedure was then applied in living pigs to evaluate the feasibility and to observe possible adverse reactions in a 2-week follow-up.Resnlts The absolute working pressure of the negative pressure suction enteroscopy should be higher than 0.06 Mpa.The procedures were successfully completed in all 20 pig small intestines in 40 + 20 min.There was no tissue damage in the intestinal wall according to histological findings.Local glide occurred in living pig examination at 0.06 Mpa,and it disappeared when the absolute value was higher than 1.00 Mpa.The procedure was completed in 20 living pigs in 60 ± 20 min.Histological findings showed complete tissue structure,clear villi and complete submucous and muscular tissue.All pigs could take food at recovery from anaesthesia.18 had bowel movement on the 2nd day after the operation,and the 2 others recovered on the 3rd day.No abdominal distension,hematochezia,or constipation was observed in 2-week follow-up.Food intake,activity,sleep,urination and defecation were all normal.Conclusion The negative pressure suction enteroscopy is effective and safe for small intestine examination,and is of good prospect.

Objective:To evaluate the feasibility and safety of total aortic arch surgery under mild hypothermicvia single upper hemisternotomy approach.Methods:From January 2019 to July 2019, 35 patients(31 male and 4 female) with Stanford A type aortic dissection were diagnosed, who were(43.7±5.7)years old. Aortic arch surgeries were carried out under mild hypothermic via single upper hemisternotomy approach and the perioperative mortality, time of cardiopulmonary bypass(CPB), aortic cross clamp(ACC), circulation arrest(CA) and morbidity of neurological dysfunction were respectively were recorded.Results:All patients were finished aortic arch surgery under mild hypothermic single upper hemisternotomy approach, with 8.6% of mortality(3 patients died perioperation). The time of CPB, ACC and CA were respectively(202±53)min, (128±28)min and(8±3)min. There were 6 cases of transient neurological dysfunction(17.1%) and 1 case of permanent neurological dysfunction(2.9%).Conclusion:Aortic arch surgery under mild hypothermic for Standford A dissectionvia single upper hemisternotomy approach is safe and feasible.

Adult ; Female ; Humans ; Male ; Middle Aged ; Palatine Tonsil ; surgery ; Temporal Bone ; abnormalities ; surgery

Objective To evaluate the feasibility and safety of Sun's procedure for Stanford A type aortic dissection through single upperhemisternotomy approach ( from the Strernal Notch to the level of fourth intercostal space ) .Methods 58 patients(39 male and 19 female) with Stanford A type aortic dissection were retrospective for this study, who were(46.3 ±9. 5) years old.Sun's prcedure were carried out through single upperhemisternotomy approach and the perioperative characteris-tics were recorded.Results All patients were finished Sun's procedure through single upperhemisternotomy approach, with 5.2％ of mortality(3 patients died postoperation).The time of cardiopulmonary bypass, aortic cross clamp and circulation ar-rest were respectively(181.6 ±25.5)min,(114.2 ±19.8)min and(29.0 ±5.8)min.Non serious adverse event were found after 2 to 15 months follow-up.Conclusion Sun's procedure through single upperhemisternotomy approach was one of the safe and feasible treatment for Stanford A type aortic dissection , which can reduce the operative wound and improve the patients ' satisfactory.

OBJECTIVE: Research for surgical methods and experience of sinus fibrous dysplasia treatment by auxiliary nasal endoscopy image navigation. METHOD: Retrospective analysis of the records of 4 patients from March 2006 to December 2011 who were diagnosed as fibrous dysplasia of nasal sinuses, 2 male and 2 female, aged from 19 yrs to 55 yrs and average age was 38 yrs; 3 of them were destroyed of sinus floor, the other who had the bone fiber of sphenoidal sinus. By using 16 multi slice spiral CT and 3D reconstruction, the 4 cases were all treated by drill grinding abnormal fibrous tissue in the nasal sinus cavities with auxiliary nasal endoscopy image navigation, to achieve contour in the bone fiber cavities. RESULT: Lesions of 4 cases were accurately found and grinded under auxiliary nasal endoscopy image navigation. For 3 patients, headache, nasal congestion, facial discomfort and other symptoms have gradually disappeared after operation. However, one patient with sinus floor destroyed suffered facial swelling and pain 3 month after operation, with ineffective conservation treatment outcomes. After CT re-scan, it showed that there were a large number of bone residue in maxillary sinus during the re-operation. The reason was that the surgical cavity was not rinsed off which leaded to maxillary sinus congestion. With the methods of expansion of the maxillary sinus, flushing the cavity, and postoperative nasal and maxillary sinus rinse, symptoms disappeared 1 month later without recurrence. CONCLUSION Patients with fibrous dysplasia of nasal sinuses are treated thoroughly, securely, and effectually, by drill grinding abnormal fibrous tissue in the nasal sinus cavities with auxiliary nasal endoscopy image navigation, to achieve contour in the bone fiber cavities.
Adult ; Endoscopy ; methods ; Female ; Fibrous Dysplasia of Bone ; surgery ; Humans ; Image Processing, Computer-Assisted ; Male ; Middle Aged ; Paranasal Sinus Diseases ; surgery ; Retrospective Studies ; Tomography, X-Ray Computed ; Young Adult

Objective:To investigate the characteristic of ultrasound in diagnosing fetal intestinal volvulus.Methods:From April 2015 to May 2018, four cases of fetal intestinal volvulus indicated by prenatal ultrasound in the Affiliated Hospital of Jining Medical College and confirmed either by autopsy or by postnatal surgery, were retrospectively analyzed. A total of 11 ultrasound scans had been performed on the four cases, and the dynamic and static ultrasound features were summarized. Pregnancy outcomes and concomitant malformations were also followed up. Descriptive statistical analysis was used.Results:The direct ultrasonographic patterns of intestinal volvulus in the four cases were "whirlpool" signs (six times), clockwise wrapping of branch vessels of the superior mesenteric vein and the superior mesenteric artery (five times), and "coffee bean" sign (three times); the indirect signs included dilated small intestine (11 times), ascites (eight times), polyhydramnios (five times), peritoneal calcification(four times), pseudocysts(once). Out of the 11 ultrasound examinations, early-stage volvulus was detected on three occasions, all with "whirlpool" signs and clockwise wrapping of branch vessels of the superior mesenteric vein and the superior mesenteric artery. As the disease progressed to the meconium peritonitis stage, indirect signs emerged while the initial two signs can diminished. Postnatal surgery or autopsy after labor induction confirmed the segmental volvulus in the four cases, of which three were complicated by intestinal atresia. No intestinal malrotation was found.Conclusion:Typical direct signs and atypical indirect signs could be identified in the sonogram findings of fetal volvulus, and are important for diagnosing fetal volvulus.

OBJECTIVETo analyze clinical features and mutation in MYH9 gene for a family featuring autosomal dominant May-Hegglin anomaly.

METHODSClinical and pathological features of all family members were analyzed. Blood samples were collected from the proband and other family members, and genomic DNA was extracted. Potential mutations of MYH9 gene exons 10, 25, 26, 30, 38 and 40 were screened with PCR and direct sequencing. After a mutation was identified in the proband, other affected members as well as healthy members from this family were analyzed with a pair of primers to amplify the mutant site. The PCR products were digested with Taq I enzyme and analyzed with agarose gel electrophoresis.

RESULTSAll affected members had bleeding tendency and typical features including giant platelets, thrombocytopenia and characteristic Dohle body-like leukocyte inclusions. A heterozygous missense mutation c.5521G>A (p.Glu1841Lys) in exon 38 of the MYH9 gene was identified in all affected members from this family.

CONCLUSIONThe variant, c.5521G>A (p.Glu1841Lys) of MYH9, has co-segregated with the phenotype in the family. The mutant site is a hot spot in Chinese population.

Asian Continental Ancestry Group ; genetics ; Base Sequence ; China ; Exons ; Female ; Genes, Dominant ; Hearing Loss, Sensorineural ; Humans ; Male ; Molecular Motor Proteins ; genetics ; Mutation ; Myosin Heavy Chains ; genetics ; Pedigree ; Phenotype ; Thrombocytopenia ; diagnosis ; genetics