AIM: To prepare the asperosaponin—phospholipid compound to improve the bioavailability of asperosaponin in rat intestine in situ. METHODS: Ratio of asperosaponin to phospholipid was chosen as index to screen the optimal proportion based on the orthogonal design.Rat intestine perfusion was use to compare aspersaponin absorption under with and without phospholipid. RESULTS: Asperosaponin—phospholipid compound showed the markedly improvement in absorption capacity and absorption rate. CONCLUSION: The experiment recommends that asperosaponin in the combination with phospholipid is better than only used asperosaponin concerning bioavailability.

Zinc-α2-glycoprotein (ZAG), a new adipokine, plays an important role in the development of cancer cachexia by decreasing lipid content in body via promoting lipid mobilization and utilization. The lipolytic effect of ZAG is achieved by increasing the activity of hormone sensitive lipase, activating β3 adrenergic receptors,and up-regulating cyclic adenosine monophosphate. In addition, ZAG increases adiponectin expression and decreases leptin expression in adipocytes and adipose tissue. The ZAG expression is regulated by glucocorticoid, peroxisome proliferator-activated receptor gamma agonist rosiglitazone, tumor necrosis factor-α and many other factors.ZAG can potentially be applied as a tumor marker, and may become a new target in the treatment of cancer cachexia.

Objective To know the implementary circs and short-dated effects of cooking stoves improvement in reducing fluoride level in the endemic fluorosis areas and to offer the background information and scientific basis for continuing the development of cooking stoves improvement in reducing fluoride level. Methods To do the surveys in the households, 769 families were selected and investigated from 38 000 peasant families in the program, by cluster sampling with 2% sample rate and 70 families were randomly selected from them, the fluoride levels in the air, foodstuff, non-staple food was determined. Results 98.7% of the families in the cooking stoves improvement areas could rightly use the improved stove. The fluoride levels in corn and hot peppers were greatly improved in county A compared with that in 2000, but the fluoride levels in the air, foodstuff, non-staple food still exceeded the standard limit, not all reached the national standard. Conclusion The headstream interventions measure in coal-burning type endemic fluorosis areas is effective. Controlling the fluoride pollution of capsicum and tea is necessary, the production methods must be improved. The health education and management should be strengthened for reducing fluoride level in the investigated areas.

Objective To research the impact of formaldehyde air pollution on fractal characteristics of Osmanthus Fragrans leaves and the application of fractal theory in formaldehyde air pollution monitoring.Methods As the experimental materials, dwarfed potted Osmanthus Fragrans were exposed to formaldehyde at different doses of 0.001,0.005,0.025,0.125,0.625 mg/m3 by air.After new leaves matured, the vein samples were first produced;then their fractal dimension was analyzed with fractal theory and the computer system.Thirdly, the quantitative analysis of the variability degree was made and dose-response relationship among the newborn leaves of every dose group was researched.Results The corresponding correlation coefficients of leaf fractal dimension were more than 0.997 0(P

AIM: To compare the dissolution of asperosaponin Ⅵ between the ultra-micro powder and the fine powder of Radix Dipsaci.METHODS : The real contents,in vitro release and releasing rate of asperosaponin Ⅵ were determined by HPLC for the ultra-micro powder and the fine powder.RESULTS: In the ultra- micro powder and the ordinary powder,the real content of asperosaponin Ⅵ were 4.87%,4.74%,respectively; in vitro release in 1 h were 48.2 mg/g,47.5 mg/g,respectively; releasing rate parameter T_(0.9) were 0.23 min,10.41 min,respectively.CONCLUSION: The ultra- micro porphyrization could not influent the real content and in vitro release of asperosaponin Ⅵ in Radix Dipsaci.But it could improve the releasing rate of asperosaponin Ⅵ.

Objective To study the toxicity and its mechanism of Radix Aconitii from genetic and molecular levels.Methods According to the requirements of ICH,the acute toxicity experiment was carried out under the condition of SPF by ig administration of the decoction of Radix Aconitii to mice.Gene expression profiling was used to describe whole-genome of five organs in mice,the related data were analyzed by using bioinformatics statistics,such as Cluster,GO,and Pathway,and the results were validated through quantitative PCR.Results The effects of Radix Aconitii on the key genes of the Focal adhesion pathway of mice,such as ECM,FAK,Cdc42,were remarkably.Conclusion The reason why Radix Aconitii produces the toxicity is probably that Radix Aconiti could cause the toxicity of Focal adhesion signal pathway through influencing the key genes.

AIM:To compare the dissolution of asperosaponin Ⅵ between the ultra-micro powder and the fine powder of Radix Dipsaci.METHODS:The real contents,in vitro release and releasing rate of asperosaponin Ⅵwere determined by HPLC for the ultra-micro powder and the fine powder.RESULTS:In the ultra-micro powder and the ordinary powder,the real content of asperosaponin Ⅵ were 4.87%,4.74%,respectively;in vitro release in 1 h were 48.2 mg/g,47.5 mg/g,respectively;releasing rate parameter T_ 0.9 were 0.23 min,10.41 min,respectively.CONCLUSION:The ultra-micro porphyrization could not influent the real content and in vitro release of asperosaponin Ⅵ in Radix Dipsaci.But it could improve the releasing rate of asperosaponin Ⅵ.

Objective To obsrve the effects of thalidomide on angiogenesis, growth and metastasis of human hepatocellular carcinoma (HCC) in nude mice. Methods The model bearing HCC was set up in 24 nude mice, and then the rats were randomly divided into therapy group and control group, each containing 12 animal. Thalidomide (200mg/kg/d) dissolved in 0.5% CMC suspension was administered intraperitoneally in the therapy group, and equivalent 0.5% CMC was administered in the control group. Mice were sacrificed at the 30th day after administration, the size and weight of tumors and metastatic condition in liver and lung were determined, levels of CD34 and VEGF mRNA in tumor tissues were detected by immunohistochemical and semi-quantitative RT-PCR, respectively, and microvessel density (MVD) in tumor tissues was also counted. Results MVD and VEGF mRNA level in therapy group were lower than those in control group (P

Objective To select a practical and reliable method in cleaning and disinfecting endo-scope, and to investigate the efficacy of endoscope disinfection. Methods A study was done on the disinfection efficacy using or not using enzyme cleaning plus 2% glutaraldehyde immersion, and disinfection efficacy of automated washer/disinfectors for endoscopes at tertiary hospitals in Beijing. Results In 301 cases of en-doscopes cleaned with enzyme ( Epizyme rapid multi enzyme cleanse) and immersed in 2% glutaraldehyde for 7 minutes, culture was negative in 283 cases (94. 02% ) ; in 155 cases without enzyme cleaning but with immersion for 7 minutes in 2% glutaraldehyde, culture was negative in 137 cases (88. 39 % ). Taking 20 or less bacteria per endoscope as the eligibility criterion, there were 298(99.00% ) and 147(94. 84% ) cases eligible in enzyme treated and enzyme untreated group respectively , P

Objective To study the effects of three kinds of endoscopic therapy on gastric varices. Methods In this study, the efficacies of endoscopic band ligation ( EVL) , endoscopic injection of tissue adhesives (histoacryl) and sclerotic agent (EIS) for gastric varices were assessed. Forty consecutive, non-randomized patients with gastric varices were prospectively studied. EVL was done for 11 cases, Histoacryl 15 cases, and EIS 14 cases, to control and prevent relapse of bleeding. Results In the following-up ( six months) period, the effective rate of EVL, histoacryl and EIS were 100. 0% , 100. 0% and 72. 7% ; the e-radicative rate 25. 0% , 30. 8% ,0 (P