Objective To explore the clinical value via comparison of umbilical incision and traditional right upper quadrant abdominal transverse incision of congenital hypertrophic pyloric stenosis.Methods According to the digital table,38 children with congenital hypertrophic pyloric stenosis were divided into two groups,the study group (n=19)was given umbilical transverse incision,the control group(n=19)was given right upper quadrant abdominal transverse incision.The effect of the two groups were compared.Results In the study group,the operate time was (20.4 ±1.47)min,incision scar healing time was (5.53 ±0.51)d,which were significantly lower than (24.00 ± 1.36)min and (7.26 ±0.45)d of the control group(t=7.835,11.090,all P<0.05).The length of incision scar [(0.53 ±0.015)cm]was obvious shorter than that of the control group[(2.03 ±0.12)cm](t=54.070,P<0.05). The postoperative eating time[(23.84 ±0.96)h]and hospitalization time[(9.37 ±0.83)d]of the study group had no difference with the control group of[(24.11 ±0.81)h and (9.42 ±0.84)d](t=0.936,0.184,all P>0.05). Followed up for 6-8 months,all children had good growth and development,but abdominal scar was obvious in the control group,and the study group had no obvious scar.Conclusion Umbilical incision for the treatment of congenital hypertrophic pyloric stenosis does not prolong the operation time,and has rapid postoperative recovery and better beau-ty effective than the traditional operation,.It is suitable for primary hospitals with no condition to carry out laparoscop-ic operation.

Relation between change of capillary pressure, survival length in bi pedicled separation delay flap was observed. Bi polar, bi pedicled separation delay flaps were designed on the backs of New Zealand rabbits. Medical silicon membrane was used as separative material. Values of capillary pressure were measured at proximal, middle and distal points. Survival length increased with delayed time, capillary pressure at middle was lower in the bi pedicled separation delay flap. It is concluded that capillary pressure is increased greatly by separation delay, and there is a linear relation between capillary pressure and survival length of flaps.

[Objective]To sturdy the biological responses of osteoblast-like cells in different maturation stages under shear flow.[Method]Flow chamber system was used to apply shear flow stimulation of 4.62dyn?s/cm~2 for 2 hours on osteoblast-like cell MC3T3-E1 of different maturation stage induced by osteogenic media,and then samples for dsDNA content and ALP activity analysis were collected.[Result]On 24 hours after simulation,there was not obvious change of ALP activity with non-osteogenic inducing group,an increase was observed in the group of 4 days of differentiation,while an decrease in the group of 8 days of differentiation.[Conclusion]Biological responses of osteoblast-like cells in different maturation stages under shear flow is quite different,and this may be an important role in bone growth and reconstruction.

The effect of the selective debridement with the aid of the arthroscope performed under local anesthesia for osfeoarthritis of 296 knees in 260 patients was studied. The pathological changes of osteoarthritis under the arthroscopy include hyperplasia of synovial membrane, degeneration of cartilage or exposure of subchondral bone on the stress areas of the femur condyles and patella , wear and tear of meniscus, formation of osteophyte and loose body. The debridement included the removal of the loose body, hyperplasia of synovial membrane, degenerated cartilage, and the torn meniscus. Many factors,including age of the patients, selection of the indication, stress of the knee joint, and the degeneration of cartilage,may influence the curative effect. After selecfive debridement and lavage for osteoarthritis of the knee joint, the majority of patients were released from pain, with restoration of function and range of motion, and the development of pathological process was retarded. Good and excellent results constitute 74.7% of the patients.

Bi-polar,bi-pedicled separation delayed flaps were designed on the backs of New Zealand rabbits with the application of a medical silicon membrane as the separation material. Values of glucose and lactic acid, PtcO 2 were measured separately at proximal,middle and distal areas of the flaps after the distal pedice was cut at 0,1,4,7,14,21 days,respectively, after the formation of flaps. After flap delaying,PtcO 2 in separated flap was always lower than that of the mormal control,accompanied with glucose decrease and lactic acid increase, although there were significant differences in different areas of the flap. The survived area of the flap increased after 7 days. Furthermore,all the length of the flap survived after 21 days,although the level of glucose in the flap was still lower and lactic acid was still higher.The results suggested that by separation and delaying the flap may adapt gradually to hypoxia via lowered metabolism.For flap survival,the threshold value of glucose in flap is 0 01mmol/L , lactic acid is 0 47mmol/L,and PtcO 2 is 3mmHg .

Objective To evaluate the clinical outcomes of arthroscopically assisted combined anterior and posterior cruciate ligament reconstruction, with an implant fixation of combined press-fit technique of hamstrings tendon knot and bone bolt. Methods 15 cases of combined injury of anterior and posterior cruciate ligament were reconstructed under arthroscopy. There were 10 males and 5 females, and the average age of patients was 23 years ranging from 19 to 31 years. 9 left knees and 6 right knees were involved. A hamstring was taken by standard procedure with a stripper, and pretension was performed on the harvested tendons; a bone bolt was inserted into the tendon to form a tendon knot. The bottle-neck shaped tunnel was prepared by stepping drill at the femoral side, the tendon knot and bone bolt was pulled inside of femoral tunnel, and then out of the tibial tunnel. Repeating the full extension and flexion of the knee joint for 20 times, and then the weaving suture of tendons was brought to pass underneath of tibial bridge and tightened together. Results The patients were evaluated by Lysholm knee functional scales, the average knee score were 61.5?4.6 and 92.5?3.7 respectively before and after operation, and the difference was of statistical significance(P

To evaluate the curative effect and value of arthroscopic surgery in the treatment of elbow diseases. Eighteen patients with elbow diseases (osteoarthritis, rheumatoid arthritis and articular adhesion) were treated with arthroscopic surgery from May 1999 to January 2003. The modified HSS elbow rating scale was used as the criteria of the efficacy. All patients were followed up for 3 to 34 months (an average of 13 months). Among eighteen patients, eleven showed excellent results, six good, one poor, and the satisfactory rate was 94.4%. It is concluded that arthroscopic surgery for elbow ailments is a safe and reliable method with the advantages of less trauma and earlier recovery. Injury to vessels and nerves may be avoided with the use of local anesthesia.

Objective To construct the eukaryotic expression plasmid of recombinant human hypoxia-inducible factor-1?(HIF-1?) and get ready for the coloning and expressing of HIF-1? gene. Methods The total RNA was isolated from blood cells,and cDNA library was constructed by reverse transcription PCR method.PIREGFP containing EGFP fragment and cDNA were used as templet,the three designed primers (EGFP-linker,HIF-1? upstream and HIF-? downstream)were put into,after amplification the target gene fragment was inserted into the shuttle vector T,and transfected with E.coli DH5?,the bacterial colonies containing recombinant plasmids were identified by LB/KANA-agar plate,and the recombinant plasmids were extracted and purified.All sequences amplified by PCR were confirmed by complete sequencing.The correct sequences were cloned into the pVAX1 vector.Results The amplified fragments were about 870,1 199,672 bp by PCR,they were inserted into the shuttle vector T and sequenced. The result of sequencing was identical with that provided by GenBank.The final plasmid about 1 800 bp was obtained by the identification of enzyme digestion and it had same molecular size as expected.Conclusion The eukaryotic expression plasmid pVAX1-EGFP-linker-HIF-1? of recombinant human HIF-1? is successfully constructed.

Objective To investigate the value of TAP in the early prediction of severe acute pancreatitis (SAP) and to compare it with acute physiology and chronic health evalutionⅡ (APACHEⅡ).Methods We observed the association between TAP concentration and the severity of acute pancreatitis with competitive enzyme-linked immunosorbent assay(ELISA). Urine samples were collected for the measurement of TAP concentration at admission, 24,48,and 72*!h after from 41 patients with acute pancreatitis (AP) (12 severe cases, and 29 mild) who presented within 48*!h of the onset of symptoms and from 11 control patients, while APACHEⅡ scores were recorded at 48*!h after admission. Results The TAP at admission of SAP (95*!nmol/L) was signficantly higher than that of mild (20*!nmol/L; P

Objective To study the relationship between the expression level of human telomerase reverse transcriptase (hTERT) protein and telomerase activity as well as its clinical significance in hepatocellular carcinoma (HCC). Methods Immunohistochemical SP method was employed to detect hTERT protein expression in 52 cases of HCC and paired adjacent tumor tissues, and telomerase activity was examined in these samples with telomeric repeat amplification-ELISA. Results The positive rates of hTERT protein expression and telomerase activity in HCC were 86.5%(45/52) and 80.8%(42/52), respectively, which were significantly higher compared with the adjacent tumor tissues(P