Objective To investigate the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.Methods RT-PCR was used to study the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells in vitro. Results After coculture with leukemic bone marrow stromal cells, the expressions of CRIF1 and Gadd45 mRNA in Jurkat cells were significantly higher than the control.Conclusion High mRNA levels of CRIF1 might be associated with the G0/G1 phage arrest in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.

Objective To screen and analyze the differentially expressed genes of Jurkat cells cocultured with bone marrow stromal cells(BMSCs) from leukemia patients.Methods Suppression subtractive hybridization(SSH) was employed to establish subtracted cDNA library of differentially expressed genes in Jurkat cells cocultured with BMSCs from leukemia patients in vitro.The cDNA fragments were sequenced and analyzed.Results The differentially expressed gene cDNA library of Jurkat cells cocultured with BMSCs from leukemia patients in vitro was developed by SSH.Primary screening was done by reverse northern hybridization.Thirty up-regulated and 22 down-regulated cDNA fragments were isolated and sequenced,and sequence homology was performed by BLAST in GenBank.These genes were related to cell cycle regulation,cellular apoptosis and energy metabolism.The information of differentially expressed genes in Jurkat cells implied that their secretion function,cell cycle and apoptosis were affected by BMSCs from leukemia patients.Conclusion BMSCs from leukemia patients might influence gene expression of Jurkat cells.The differentially expressed genes might be significantly associated with the protection of BMSCs from leukemia patients on Jurkat cells during Daunorubicin exposure.

Objective:To analyze essential health care equity in cities and counties of Hainan in order to provide the basis for its development. Methods:Based on health statistics of Hainan, China and OECD countries, indicators of essential health care equity in cities and counties were analyzed. Results:Equity in distribution of resources, the hospi-tal geographical distance is large difference, physician-to-nurse ratio is 1:1. 2, and the Gini coefficient is more than 0. 3. Equity in financing, health care spending per capita is less than the national level. Cities and counties with high government budgets for health are located in regional centers. The hospitalization costs compensation with new rural co-operative medical scheme is about 50%. Equity in service supply, bed occupancy rate below 90% and drugs accounted for 30% ~40% in most cities and counties. Regional differences in maternal and child health management are small, but below the national level. Conclusions:The situation of essential health care resources is related to the political and economic climate. Financing mechanisms for essential health care are imperfect;and a low supply of basic medical and public health services reduced the quality of essential health care. Suggestions:Rational distribution of essential health care resources should be promoted;essential health care financing should be increased;and the capability of essential health care supply should be improved.

Objective To evaluate the image characteristics and quality of MR plain scan with breath-holding in normal lungs. Methods There were 21 normal volunteers to be examined by MR plain scan with breath-holding using T_1WI,T_2WI,PWI fast sequences. It was required to analyze the image characteristics, to measure and calculate signal to noise ratio of the lung parenchyma and the muscle of thoracic wall individually, the contrast and contrast to noise ratio between the lung and the muscle of the thoracic wall. Results On T_1WI,The signal of lung parenchyma was weak and the beating artifacts projected to the lung fields,especially in the left inner and posterior segments. On T_2WI, the lung markings could be seen more, but the noise was obvious in the background and flowing related enhancement appeared in great vessels and heart. On PWI, the signal of lung parenchyma was homogenous and more strong without the beating artifacts of heart and great vessels. According to the order mentioned above, the signal to noise ratio were 1.68?0.21; 2.74?0.26; 4.61?0.79( F =218.06, P

Objective To investigate the incidence and clinical significance of “no flow” (NF) phenomena after primary percutaneous coronary intervention (PCI) in the treatment of acute myocardial infarction (AMI). Methods ninety nine consecutive AMI patients underwent primary PCI were devided into NF group (18 cases) and non NF group (81 cases). The incidence of NF, clinical characteristics, blush grade, ST T segment elevation, peak CPK, left ventricular function and cardiac events were analysed. Results Eighteen patients demonstated NF, the incidence of NF was 18.2%. Compared with non NF, previous MI、diabetes、anterior MI and multivessel disease were more frequently observed in the cases with NF ( P 0.05). Conclusions The incidence NF after primary PCI in the treatment of AMI is 18.2%. The patients with NF show malperfusion in the myocardial microcirculation, larger infarct size, ventricular dysfunction and poor prognosis.

Objective To observe the clinical effect of blood transfusion on earthquake victims from Wenchuan earthquake area, Sichuan province.Methods The blood routine, electrolyte and coagulation function of earthquake victims were detected.Results A total of 41(27.5%) victims received blood transfusion. The average volume of blood transfusion was 1 233 ml, and the average volume of plasma was 1 550 ml. No patient was found to have adverse transfusion reaction. There was no significant difference between the electrolytes before and after the transfusion.Conclusion Blood transfusion is important in the care of earthquake victims.

Objective To study the influence of bone marrow microenvironment on leukemic cells during chemotherapy and the influence on leukemic cells' drug-resistance and anti-apoptosis. Methods Normal bone marrow stromal cells were isolated using Percoll, and then were cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. Apoptosis percentage of Jurkat cells labelled with Annexin V/PI were detected by FCM. Results Apoptosis percentage of Jurkat cells cocultured with bone marrow stromal cells for 24 hours was lower than those maintained in suspension (P

Objective To investigate the relationship between the maturity and function of dendritic cells (DC) and hepatitis B virus covalently closed circular DNA (HBV cccDNA) load in the peripheral blood mononuclear cells (PBMC)/monocyte-derived DC in patients with chronic hepatitis B (CHB). Methods The peripheral blood samples were collected from 29 patients with CHB and 10healthy controls. PBMC were isolated freshly and induced with granulocyte/macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). A large amount of DC were harvested after seven days of culture. The expressions of CD209, CD80, CD86, human leucocyte antigen (HLA)-DR and CD1a of DC were analyzed by flow cytometry. The HBV cccDNA load in PBMC and DC were measured by real-time polymerase chain reaction (PCR). The interleukin-12 (IL-12) level in the culture supernatant of DC was determined by enzyme linked immunosorbent assay (ELISA). The effects on T lymphocyte proliferation induced by DC were tested by mixed lymphocyte reaction (MLR). The data was compared by t test and analysis of variance. Results HBV cccDNA could be detected in PBMC from 16 patients, but not in DC from all 29 patients. HBV cccDNA load was all negatively correlated with the expressions of CD209 (r= -0. 793, P＜0.01), CD80 (r= -0. 581,P＜0.05), CD86 (r=-0. 698, P＜0.01), HLA-DR (r=-0. 817, P＜0.01), CD1a (r=-0. 734, P＜0.01), IL-12 level (r=-0. 632, P＜0.05) and allogenic T lymphocyte proliferation induced by DC (r=-0. 617, P＜0.05). The expressions of CD209, CD80, CD86, CD1a and HLA-DR on DC,IL-12 level in culture supernatant of DC and the allogenic T lymphocyte proliferation induced by DC in patients with positive PBMC HBV cccDNA were all significantly lower compared to those in healthy controls, and the changes of the parameters mentioned above were greater in PBMC HBV cccDNA positive patients than those in PBMC HBV cccDNA negative patients (P ＜ 0. 05 or P ＜ 0. 01).Conclusions The function and maturity of DC are impaired in CHB patients. HBV cccDNA can be detected in PBMC from CHB patients. Moreover, the higher PBMC HBV cccDNA is, the worse DC function and maturity are, which could be one of the important mechanisms of HBV persistent infection.

Objective To observe the effects of Shenmai injection on cardiac hemodynamics of isolated heart in rats of myocardial infarction model. Methods 30 myocardial infarction rat models created by ligation of left anterior descending coronary artery were randomly divided into a model group, a captopril group and a Shenmai group. 24 hours after successfully setup the models, all rat models were executed and their hearts were taken. Langendorff isolated heart perfusion method was adopted, and each group was perfused with 0.05mol/L amount of corresponding medicine, the model group was perfused with KH nutritive medium. left ventricular peak pressure(LVSP)and left ventricular ejection fraction(LVEF), left ventricular pressure increase/decrease rate(dp/dtmax±LV)(mmHg/s)and left ventricular end systolic diameter(LVESD)(%)and left ventricular end diastolic diameter(LVEDD)(%) was observed 5 min before the perfusion and 0.5 h, 1 h, 2 h after the perfusion. Results LVSP(9.47%± 0.15%vs. 3.34%± 0.05%),(11.25%± 1.31%vs. 3.79%± 0.04%) and LVEF reducing rate (7.44%± 0.10%vs. 4.94%± 0.04%), (10.24%± 0.31%vs. 5.34%± 0.05%) were elevated, and ±dp/dtmax(5 011 ± 253 mmHg/s vs. 5 827 ± 227 mmHg/s), (4 732 ± 212 mmHg/s vs. 5 837 ± 254 mmHg/s);(3 139 ± 127 mmHg/s vs. 4 722 ± 231 mmHg/s), (2 997 ± 125 mmHg/s vs. 4 793 ± 241 mmHg/s) was reduced in the Shenmai group 1 and 2 hours after the administration, which showed statistical difference compared to the model group (P<0.05). Conclusions Shenmai injection reduce myocardial contractility and intraventricular pressure of isolated heart o myocardial infarction rat models.

OBJECTIVE:To gain clear idea of influence of multiorigins of Cordyc ep s on quality and nature of this drug.METHODS:The mannitol contents in the Cordy ceps samples collected from different growing areas were determined and compared ,and their MDR(Mutual Different Rate)was analyzed.RESULTS:The MDRs among specim ens from Sichuan,Tibet and Yunnan were 0.012～0.32 and the MDRs among the Cordyc eps samples from different micro-ecological environment were 0.037～1.36.CONCLU SION:On the mannitol content in Cordyceps,the effect of the species diversity a nd the micro-ecological environment might exceed that of district and climate d ifference.