OBJECTIVE:To investigate the utilization and trend of drugs for herpes zoster patients from 22 hospitals of Shanghai area,and to provide reference for rational use of drugs.METHODS:The utilization of drugs for herpes zoster patients from 22 hospitals of Shanghai area during 2013-2015 was analyzed statistically in respects of proportion of patients,consumption sum,DDDs,DDC,etc.RESULTS:Herpes zoster was more likely to occur in adults,mostly in more than 45 year-old group (＞75％).The proportions of patients of neurotrophic antivirals,anti-herpes virus and acesodyne drugs ranked in the top three.Topical,immu nomodulatory and glucocorticoid drugs accounted for a certain proportion.Among antivirals,the consumption sum and DDDs of valaciclovir ranked the first place,and DDC of brivudine was the highest.Among acesodyne,the proportions of patients of antiepileptic drugs was the highest (13.78％),and its compound growth rate was also the highest (30.77％);the number of patients of gabapentin was the largest.Among neurotrophic drags,the proportions of patients of mecobalamine was the highest (32.98％).Among immunomodulatory drugs,the number of patients of thymalfasin was the largest,which may be irrational.CONCLUSIONS:The treatment of herpes zoster in Shanghai area is basically in accordance with the guidelines.The irrational use of immunomodulatory drugs should be paid attention to and supervised.

An intemationlly recognized systematic and preventive approach to food safety, Hazard Analysis Critical Control Points(HACCP) is scientific, simple, convenient and practical. An effective way to improve managerial efficiency and the quality of nutritional meals and to enhance patient satisfaction is to use the principles of HACCP in the following aspects: conducting quality control of nutritional meals in hospitals and determining a scientific and rational process flow; analyzing marked hazards affecting the safety of nutritional meads; proposing preventive measures; estabhshing critical control points; and specifying how to establish a threshold of, monitor, set right, verify and record the critical control points.

Vitamin D participates in a variety of biological processes. 1,25-dihydroxyvitamin D [1,25-(OH)2D]is the active form of vitamin D. Besides its classical role in bone and calcium homeostasis, 1,25-(OH)2D has many non-classical functions such as regulating proliferation and differentiation of cells in various tissues, including tumor cells as well. However, the role of 1,25-(OH)2D in thyroid cancer is not clear yet. There are three integral components of the vitamin D pathway in thyroid cells. These components are the vitamin D receptor, 1α-hydroxylase (CYP27B1), and 24-hydroxylase (CYP24A1). Differences in the expression levels of these components in normal thyroid tissue and thyroid cancer tissue may offer clues and lay the foundation for study of the inhibitory function in the pathogenesis of thyroid cancer.This review focuses on the insights gained in the elucidation of the role of 1,25-(OH)2D pathway in thyroid cancer, one of the most common endocrine malignancies.

BACKGROUND:Stem cels have multi-directional differentiation and self-replication abilities, under certain conditions, which can differentiate into myocardial cels to repair the damaged myocardium. OBJECTIVE: To investigate the effects of transplantation of umbilical cord mesenchymal stem cels derived at different gestational weeks on infarct size and angiogenesis in the infarct region of experimental rabbits with myocardial infarction. METHODS: Ten ful-term umbilical cord samples and 10 umbilical cord samples of aborted fetuses at 10-12 gestation weeks were selected to in vitro isolate umbilical cord mesenchymal stem cels that were subjected to BrdU labeling. HLA-G expression was detected in the cels. Thirty white rabbits were selected to make myocardial infarction models, and 2 weeks after modeling, the model rabbits were randomized into aborted cel transplantation group, ful-term cel transplantation group and control group (n=10 per group). Then, BrdU-labeled cels were injected correspondingly into the infarct region of rabbits in the two cel transplantation groups. Rabbits in the control group were subjected to an equal volume of serum-free. Four weeks after transplantation, heart function of rabbits was monitored using electrocardiogram, and myocardial tissues were taken to measure infarct size and blood capilary density. RESULTS AND CONCLUSION: HLA-G expression was different in different sources of umbilical cord mesenchymal stem cels: high HLA-G expression was found in the aborted umbilical cord mesenchymal stem cels, and meanwhile, low HLA-G expression was found in the ful-term umbilical cord mesenchymal stem cels. Compared with the control group, the left ventricular end-diastolic volume and left ventricular ejection fraction of aborted and ful-term cel transplantation groups were significantly improved, especialy in the aborted cel transplantation group (P < 0.05). BrdU-positive cels were found in the infarct site in both transplantation groups. Compared with the control group, the infarct size and capilary density were improved most significantly in the aborted cel transplantation group folowed by the ful-term cel transplantation group (P < 0.05). Electrocardiogram findings showed significant improvement in both cel transplantation groups compared with the control group (P < 0.05), especialy in the aborted cel transplantation group. These findings indicate that umbilical cord mesenchymal stem cels derived at low gestational weeks improve the heart function more significantly than the ful-term umbilical cord mesenchymal stem cels, which have the potential to become a better source of cardiomyocytes for transplantation.

BACKGROUND:Human umbilical cord blood-derived mesenchymal stem cels are able to repair and regenerate the injured myocardium, which is a new therapy for myocardial infarctionvia transplantation. OBJECTIVE:To explore the therapeutic efficacy of intracoronary injection of human umbilical cord blood-derived mesenchymal stem cels on acute myocardial infarction in rats. METHODS:Thirty-two rats were selected to make animal models of ligation of the left anterior descending coronary, and then model rats were randomized equaly to transplantation group and model group. Human umbilical cord blood-derived mesenchymal stem cels were isolated and prepared into cel suspension. Rats in the transplantation group were subjected to transplantation of human umbilical cord blood-derived mesenchymal stem cels. RESULTS AND CONCLUSION: Human umbilical cord blood-derived mesenchymal stem cels were successfuly isolated and cultured in vitro. Compared with the model group, the microvessel density, left ventricular end-systolic pressure and ±dp/dtmax were significantly increased in the transplantation group (P < 0.05), while the left ventricular end-diastolic pressure was decreased dramaticaly (P < 0.05). Electrocardiography findings showed that the heart function of rats in the transplantation group was improved slightly. These findings indicate that human umbilical cord blood-derived mesenchymal stem cels can promote myocardial angiogenesis and improve heart function of rats with myocardial infarction.

BACKGROUND:A large amount of inflammatory mediators from myocardial cels are secreted in response to myocardial injury after myocardial ischemia. Moreover, inflammatory cytokines in infarction and ischemia regions contribute to myocardial tissue repair and adaptation.
OBJECTIVE:To investigate the effect of tumor necrosis factor-α (TNF-α) pretreatment on cardiac function of myocardial infarction rabbits undergoing umbilical cord blood mesenchymal stem cel transplantation.
METHODS: Thirty-six white rabbits were equally randomized into sham, model, non-TNF-α, and TNF-αgroups. Animal models of myocardial infarction were made in the latter three groups. Twenty-four hours after modeling, PBS, umbilical cord blood mesenchymal stem cells pretreated with or without TNF-α were injected at infarct center and border, respectively, in the model, TNF-α and non-TNF-α groups.
RESULTS AND CONCLUSION: Model rabbits in the TNF-α and non-TNF-α groups showed better cardiac function and lower size of myocardial infarction and fibrosis than those in the model group. Compared with the non-TNF-α group, moreover, the TNF-α group showed better outcomes in these indicators. These findings indicate that TNF-α pretreatment canmarkedly improve the therapeutic efficacy of umbilical cord blood mesenchymal stem cel transplantation on myocardial infarction.

Objective:To observe the effect of timosaponin AⅢon the proliferation of cultured melanoma B16 and A375 cells and nitrite produced from macrophage activation. Methods:MTT assay was adopted to detect the cell growth inhibition. The morphological changes of B16 and A375 cells were observed under an inverted microscope. Nitrite production of activated mouse macrophages induced by lipopolysaccharide ( LPS) plus IFN-γ was measured by Griess assay. Results: Timosaponin AⅢ could significantly inhibit the growth of B16 cells at the concentration of 16 μmol·L-1 after the 48- and 72-hour treatment, and significantly inhibit the growth of A375 cells at the concentration ranged from 4 to 16 μmol·L-1 after the 48-and 72-hour treatment. Shrinkage, vacuoles and necrosis of B16 and A375 cells were observed after the 48-and 72-hour treatment by 16μmol·L-1 timosaponin AⅢ,the other concentration of timosaponin AⅢ showed no notable effect on B16 cells and vacuoles of A375 cells appeared at the concentration from 4 to 16 μmol· L-1. Compared with RAW 264. 7 stimulated LPS plus IFN-γ,timosaponin AⅢ could significant inhibit nitrite production of macro-phage inflammatory cell model at the concentration of 10μmol·L-1(P<0. 01). Conclusion:Timosaponin AⅢcan inhibit the prolif-eration of melanoma B16 and A375 cells and macrophage inflammation,suggesting it has anti-tumor and anti-inflammatory effects . The anti-tumor effect of timosaponin AⅢ may be related to the inhibition of tumor inflammation.

As a special profession in our society,doctor has its special rights.An investigation of Jinzhou,Chaoyang,Huludao,Panjin,Fuxin and Tieling,Anshan in the Liaoning province review that violation of doctors' right occurs from time to time.There are several reasons for the violation,mainly coming from doctors,patients and their relatives,guidance of social media and social causes in depth.

Objective To explore the expression and clinical significance of survivin protein and proliferation cell nuclear antigen(PCNA) in human nasopharyngeal carcinoma (NPC). Methods The expression of survivin protein and PCNA was detected by SP immunohistochemical technique in 96 cases of NPC tissues. Results The positive rate of survivin expression was 75.0% in PNC. Labeling idex(LI) of PCNA in survine positive NPC was higher than that in survivin negative NPC. The positive rate of survivin expression in NPC with neck lymph node metastasis was higher than that in NPC without neck lymph node metastasis. The positive rate of survivin expression in NPC patients with less than 5-year survival rate was higher than that in NPC patients with over 5-year survival rate. Conclusion NPC had survivin expression, and survivin expression in NPC was positive related with PCNA expression. The survivin protein might promote the neck lymph node metastasis of NPC cells. The prognosis of the patients with survivin positive NPC was worse.