Objective To detect the expression of Livin in gastric cancer tissues and cells, and investigate its relation to tumorigenesis and progression of gastric cancer cells. Methods (1) The expression of Livin in gastric cancer tissues was located by immunocytochemisty. (2) Livin mRNA and protein expression in adjacent tissues of carcinoma, gastric ulcers and gastric cancer tissues were detected by RT-PCR and Western blot. Results The expression of Livin in the nucleus and cytoplasm of gastric cancer cell line SGC-7901 was observed. The positive rate of Livin expression in the gastric cancer tissues was 67% (20/30). There were no expression of Livin-α and Livin-β in the adjacent tissues of carcinoma and the gastric ulcers. The positive rate of Livin-α expression in the gastric cancer tissues was positively correlated with TNM stage of gastric cancer (x2=11.719, P<0.05). The expression rate of Livin in the differentiation and location of gastric cancer, age and gender of patients had no statistical difference (x2=1.607, 0.000, 0.156, 0.419, P>0.05). Conclusion Correlation of high Livin-α expression and pathologic staging of gastric cancer indicates that Livin-α may play an important role in the carcinogenesis and progression of gastric cancer.

Objective To establish a method for simulataneous determination of 18 free amino acids in snake venom. Methods Preparation of free amino acid samples by membrane. HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent, samples were analyzed on Ultimate LP-C18 column with gradient elution column of 0.05 mol/L sodium acetate buffer and methanol-acetonitrile- water (40:40:20), and current speed was1.0 mL/min, and the column temperature was set at 35℃, and detection wavelength was 254nm. Results The 18 kinds of amino acids showed a good linearity with the correlation coefficients ≥0.99. The recovery rate was 74.59%~110.62%. Snake venom contained 17 kinds of amino acids, the total content of amino acids was 0.2%. Conclusion The method was accurate, reproducible and reliable, and can be used for the determination of amino acids in snake venom and related products.

Objective To explore the expression and significance of Annexin Ⅱ protein in ovarian adenocar-einoma.Methods The expressions of Annexin Ⅱ protein were detected in 48 cases of ovarian adenocarcinoma and 10 cases of ovarian adenoma by SP immunohistochemistry.Results The positive rates of expression of Annexin Ⅱprotein in ovarian adenoma were 50.00% (5/10), and were 81.25% (39/48) in ovarian adenocareinoma (χ2 =4.414, P <0.05), in well and moderately-differentiated, poorly differentiated ovarian serous adenocarcinoma were 93.33% (14/15) and 78.95% (15/19) respectively (χ2 = 1.383 ,P0.05 ), and on the Ⅰ and Ⅱ stage, Ⅲ and Ⅳ stage of ovarian serous adenocarcinoma were 60.00% (6/10) and 95.83% ( 23/24 ) respectively (χ2=7.226, P <0.05).The positive rate in the group of ovarian serous adenocarcinoma with lymph node metastasis was 90.00% (27/30), while 50.00% (2/4) in the group without lymph node metastasis (χ2=4.502, P<0.05).Conclusion The positive expression of Annexin Ⅱ protein may be correlated with the carcinogenesis, development and lymph node metastasis of ovarian serous adenocarcinoma.

Objective To investigate the dynamic expression of SOCS-3, TNF-α in live and spleen of mice induced by AOPP, and to discuss the mechanism of MODS induced by AOPP, so that to give some intervention method over MODS in the future. Method Thirty-six adult BALB/c mice were divided into 3 groups randomly: AOPP group (n=12). Water normal group (n=12) and normal group (n=12). After post poison 2 hours, 6 hours, 12 hours and 24 hours,the fiver and the spleen tissue were taken out. The expression of SOCS-3 and TNF-α were detected by RT-PCR. And data were analyzed with ANOVA. Results After AOPP of 2,6,12,24 hours, the mRNA expression of SOCS-3 increased obviously in the liver and spleen compared with the normal group (P<0.05), it reached peak at the 24th hour in liver,and reached peak at the 12th hour in spleen, then descended at 24th hour (P<0.05). The mRNA expression of TNF-α increased obviously in the liver and spleen than normal group (P<0.05), and reached peak at the 12th hour,then descended at 24th hour (P<0.05), the electro-phoresis image of RNA was 5 s 15 s and 30 s,RT-PCR amplification of β-actin showed,the expression of SOCS-3, reached peak at the 24th hour in liver and it reached peak at the 12th hour in spleen, then descended at 24th hour (P<0.05), TNF-α reached peak at 12th hour in the liver and spleen, then descended at 24th hour. By statistic analysis, positive correlation was indicated among SOCS-3 and TNF-α mRNA expression in liver (y=0.089+0.758x, r=0.939, F=252. 168, P<0.01) positive correlation was indicated among socs-3 and TNF-α mRNA expression in spleen (y=0.057+0.361x,r=0.953,F=336.122, P<0.01). Conclusions At different point of time after Aopp,the mRNA expression of socs-3 and TNF-α showe the same trend in liver and spleen,the levels of SOCS-3, TNF-α all increase significenfly.

Objective 　To investigate the prevalence, characteristics and correlates of depressive symptoms in first episode schizophrenic patients. Methods　To examine 164 first episode schizophrenic patients at the time of admission and at 3,6,9, and 12 months after starting treatment using the HAMD, BPRS, the Chinese version of SANS, CGI and GAF. Results　71% of the patients had depressive symptoms (mild or more) at damission, but the prevalence of depressive symptoms dropped to a mean of 12% during the recovery period. The most prominent depressive symptoms during the acute phase of schizophrenia were ‘cognitive disturbance’ and ‘retardation’ (the respective subscales constituted 35% and 29% of the total HAMD score on admission). Depressive symptoms improved in parallel with the schizophrenic illness. The severity of depressive symptoms was not related to gender, age of onset, educational level, duration of prodromal period or duration of illness. At admission the severity of depressive symptoms was only related to the BPRS anxiety and depression subscale score, but during the recovery period the HAMD total score was significantly correlated with all of the other clinical scales. The level of depressive symptoms at admission and at three months after starting treatment was not related to the subsequent course of positive or negative symptoms. Conclusions　Depressive symptoms appeared to be a separate symptom cluster during the acute phase of first episode schizophrenia. The severity of depressive symptoms did not predict the clinical outcome of first episode schizophrenic patients.

Objective: To improve clinical utility of the Nurses Observation Sca le for Inpatient Evaluation (NOSIE-30) in China. Methods: Based on the original scale, which only provides names and ranked scores for items, we developed clear definitions for each item and anchoring descriptions for score rating. 164 firs t-episode schizophrenic patients were evaluated with this scale every two weeks after admission. A total of 994 evaluations were completed. The collected data w ere randomly divided into two independent data sets. The number and content of t he subscales were revised based on principal component factor analysis and item analysis of the first data set. The revised scale was then evaluated and compare d with the original 7-subscale version of the instrument using the second data s et. Results: Factor analysis found that the factor structu re of the revised scal e was markedly different from that of the original instrument. Item analysis res ulted in elimination of 30 original items and redistribution of the remaining 26 items into 5 subscales. Comparison of the revised scale with the original instr ument revealed better psychometric properties for the former than the latter.Conclusion: The subscale scores of the original 7-subscale v ersion of NOSIE-30 did not reflect independent symptom clusters in Chinese schizophrenic inpatients. T o improve the usefulness of the scale in China, the present study recommended a better revised scale with re-definition of items into 5 subscales.

Objective:To select suitable chemotherapy for cervical cancer patients by ATP-tumor chemosensitivity assay.
Methods:Seventy-two hospitalized patients with cervical cancer between July 2007 and October 2009 were enrolled. The patients were randomly divided into a trial group (n=35) and a control group(n=37). ATP-TCA was used to detect the sensitivity of 35 samples of cervical cancer in the trial group to 6 combined chemotherapy regimens. The chemotherapy regimen in the trial group was confirmed by the results of susceptibility testing and that in the control group was confirmed by clinical experience. One-year recurrence rate and 3-year survival rate of two groups were compared after 3 year follow-up.
Results:ATP-TCA was measured in 32 of the 35 patients in the trial group. The sensitive patients for paclitaxel+carboplatin, paclitaxel+oxaliplatin, bleomycin+ifosfamide+cisplatin, bleomycin+vincristine+cisplatin, fluorouracil+cisplatin, and gemcitabine+cisplatin were 20, 18, 17, 18, 17, and 21, respectively. There was no significant difference in the 1-year recurrence between the two groups (P>0.05), while the 3-year survivors in the trial group were more than those in the control group (P<0.05).
Conclusion:ATP-TCA method is good for patients with cervical cancer because it is sensitive, effective, and individualized.

BACKGROUND:It remains controversial in term of efficacy for the lightweight mesh and heavyweight mesh in inguinal hernia repair.
OBJECTIVE:To compare the clinical therapeutic effects of lightweight mesh and heavyweight mesh in open methods of inguinal hernia repair with Meta-analysis.
METHODS:Comprehensive electronic search strategies were developed using the fol owing electronic databases:PubMed, Cochrane Library, EMBASE, Medline, Ovid, CNKI, VIP, Wanfang and FMJS. The Literature published before February 2013 was searched. The randomized control ed trials about comparing lightweight mesh and heavyweight mesh in open methods of inguinal hernia repair were included. A data-extraction sheet was developed based on the preset standards. The data from eligible studies were pooled using RevMan5.1 software through Meta-analysis.
RESULTS AND CONCLUSION:Eighteen trials with a total of 4 450 hernias met the inclusion criteria. The meta-analysis showed that there was a statistical difference between lightweight mesh group and the heavyweight mesh group on short-term pain [odd ratio (OR)=0.57, 95%confidence interval (CI) (0.43, 0.74), P<0.05] and a reduced risk of developing foreign body sensations [OR=0.49, 95%CI (0.35, 0.69), P<0.05]. No significant differences were found between the two groups in recurrence rate, testicular atrophy, seroma, hematoma, wound infection, urine retention (P>0.5). According to limited evidence, there are some findings as fol ows:the lightweight mesh is of feasibility, safety and effectiveness for inguinal hernia repair. Because of the limits of sample and quality, more large-sample and high-quality trials are required to make a definite clinical evidence to use lightweight mesh for inguinal hernia repair.

Objective To explore protective effects of panaxtrial saponins (PTS) on cognition and memory of diabetic rats and to reveal its mechanism by which might involve regulating activity of astrocytes. Methods SD rats (n=24) were ran?domly assigned into control, diabetic and PTS-treated groups (n=8 in each group). Rat diabetic model was induced through streptozotocin injection intraperitoneally. Rats in control group were native rats, and rats in PTS-treated group were diabetic rats that were administered with PTS. Body weight and blood glucose were monitored through the experiments. Three months later, state of cognition was examined by methods of water maze. Hippocampal astrocyte morphology were detected by immu?nohistochemistry, and the expression of glial cell line-derived neurotrophic factor (GDNF) and glial fibrillary acidic protein (GFAP) in hippocampus were revealed by Western blot. Results Compared with control group, diabetic group showed cog?nitive dysfunction, atrophic astrocyte soma, shrinked astrocyte processes, and down-regulation of hippocampal GFAP and GDNF (P<0.05). Compared with diabetic group, PTS-treated group exhibited improved cognition and morphology of hippo?campal astrocyte, and reversed expression of GFAP and GDNF in diabetic hippocampus (P<0.05). Conclusion PTS re?versed astrocytic reactivity as well as expression of GDNF and GFAP in diabetic hippocampus and ameliorated diabetic cog?nitive dysfunction.

Objective:To explore the correlation among risk factors of hypertension and waist-to-height ratio (WHtR) in officers of regiment and above in troops stationed in Zhejiang .Methods :A total of 2641 officers of regi-ment and above from troops stationed in Zhejiang ,who participated in annual physical examination in our hospital from 2012 to 2013 ,were selected .Correlation among body mass index (BMI) ,waist circumference (WC) ,WHtR , waist/hip ratio (WHR) and blood pressure were analyzed ;receiver operator characteristic curve (ROC) was used to compare and analyze the predictive value of above four indexes on hypertension and judge the optimal cut-off point . Results :(1) Pearson correlation analysis indicated that WHtR was positively correlated with WC ,BMI ,WHR ,sys-tolic and diastolic blood pressure (r=0.25~0.94 ,P<0.005~ <0.001);(2) Among above four indexes ,WHtR had the largest area under the ROC curve 0.697 (95% CI 0.676~0.718) and the optimal cut-off point was 0.51 ;and it's 0.651 (95% CI 0.639~0.662) for BMI ,0.689 (95% CI 0.664~0.751) for WC and 0.685 (95% CI 0.672~0.698) for WHR ;for morbidity risk of hypertension ,compared with BMI ,WC and WHR ,WHtR possessed the most balanced sensitivity (60.68% ) and specificity (70.74% ) ,and the largest OR 4.12 (3.71~4.59) .Conclusion:(1) Among officers of regiment and above in troops stationed in Zhejiang ,anthropometric indexes are closely relat-ed to blood pressure ,which can be regarded as simple indexes for predicting and assessing morbidity risk of the popu-lation ;(2) Waist-to-height ratio may be the best anthropometric index evaluating risk of hypertension in these popu-lation and its optimal cuf-off point is 0.51 for predicting hypertension .