Objective To study the clinical features and operative methods for patients with hepatic tuberculosis.Methods The clinical features、diagnosis、operative methods and the location of tuberculosis focus in the liver were analysed in 8 patients. Results Difinite preoperative diagnosis could be reached in none of the 8 patients. Surgical procedure included focus remove (5 cases) and deroof plus drainage (3 cases). The diagnosis of hepatic tuberculosis was proved by pathological examination. [WT5”HZ]Conclusions Surgical resection plus anti tuberculosis drugs are the mainstay for the treatment of patients with hepatic tuberculosis.

Objective　To study the clinical features and operative methods for patients with hepatic tuberculosis.Methods　The clinical features、diagnosis、operative methods and the location of tuberculosis focus in the liver were analysed in 8 patients.Results　Difinite preoperative diagnosis could be reached in none of the 8 patients.Surgical procedure included focus remove (5 cases) and deroof plus drainage (3 cases).The diagnosis of hepatic tuberculosis was proved by pathological examination.Conclusions　Surgical resection plus anti-tuberculosis drugs are the mainstay for the treatment of patients with hepatic tuberculosis.

OBJECTIVE:To establish a bacterial endotoxin test for ofloxacin injection METHODS:Interference tests in ofloxacin injection were performed with limulus test agents provided by different factories RESULTS:Ofloxacin injection did not interfer with bacterial endotoxin CONCLUSION:Bacterial endotoxin test can take the place of pyrogen test for ofloxacin injection

Objective In order to study the effect of the Fas system on normal peripheral blood lymphocytes(PBL)activated by allospecific antigen.Methods The isolated and purified lymphocytes werc subjected to one-way mixed lymphocyte culture.Fas antigen exprexsion on the surface of T lymphocytes freshly isolated and activated was detected dynamically by using flow-cytometry(FCM).The influence of the Fas system activated or not on the rate of lymphocytic apoptosis after mixed culture was investigated by using DNA electrophoresis and TdT-mediated dUTP nick end labeling(TUNEL).Results The Fas expression on the surface of activated T lymphocytes was increased and the rate of apoptosis of the grow with addition of anti-Fas monoclonal antibody was higher than that of the group without addition of antibody(P<0.05).Conclusion It was possible to dispose of the lymphocytes activated by specific antigen in vitro by anti-Fas monoclonal antibody.

Burns, Inhalation ; Humans ; Respiratory System ; injuries

Objective To explore the effect of exogenesis VEGF 120 gene on the apoptosis of brain cells in the HIBD of newborn rats. Methods VEGF eukaryotic expression plasmid (pCDNA 3.1/r VEGF 120) was constructed by cloning rat VEGF 120 cDNA into eukaryotic expression vector pCDNA 3.1. The HIBD model was established with seven days old SD rats,and all rats were diveded into two groups at random :contral group 18 rats( every rat model was injected pCDNA 3.1 100 μg immediately after hypoxia-is-chemic.then raised seven days) and therapy group 18 rats (every rat model was injected pCDNA 3.1/ rVEGF 120 100 μg immediately after hypoxia-ischemic). Flow cytometer( FCM) was used to detect the ratio of apoptosis of brain cell. Results There was a significant decrease in the ratio of apoptosis brain cells( control group 17.505 ± 0.949; therapy group 8.93 ± 0. 332). Conclusion The VEGF gene product can reduce apoptosis of brain cells.

Objective To evaluate the performance of Sysmex XN‐9000 automated hematology analyzer .Methods According to international and domestic standards ,performance of analyzer was evaluated .Results The within‐batch and between‐batch preci‐sion ,carryover pollution rate ,linearity range and the accuracy of Sysmex XN‐9000 analyzer were all conform to related require‐ments .Leukocyte classification results compared with manual classification ,the correlation of neutrophil ,lymphocyte ,monocyte and eosinophil were fine ,but correlation of basophil was not very ideal .Conclusion The performance of Sysmex XN‐9000 analyzer could be satisfying ,could meet the needs of clinical inspection and diagnosis and treatment .

The pathogenic effects of crops from Keshan disease area were studied by rat-feeding. As double controls, another two groups were fed with crops from non-endemic area and synthetic diet. Those from disease area caused necrosis of heart muscle vascular changes of myocardial mitochondria and fatty degeneration of liver. Compared with the controls, the free radical and lipid peroxide content in heart and liver were significantly higher, while the ATP and AN contents in these organs were apparently lower. The multielement contents and its constituent patterns in the feeds, whole blood, heart, liver, kidney and brown adipose tissue of rats in experimetal group were apparently different from those in the control groups. The statistic analysis of multiple factors showed that eight elements Se, Mo, Cu, Mn, Cr, Fe, Zn and Pr might be the perilous factors, that is, under the condition of low selenium, the constituent pattern of selenium and the eight elements might be the complicated factors of endemic crops.

Objective To analyze and evaluate the characteristics of enzyme kinetics of CTX-M-14 type extended-spectrum β-lactamase(ESBL) with Pro167 residue substitution. Methods By molecular cloning and PCR techniques, CTX-M-14 gene was directionally cloned into plasmid pET28a( + ) from a clinical E. coli isolate and formed an expression vector to transform competent E. coli BL21 (DE3 ). Prol67 residue substitutions of P167G, P167Q, P167S and P167T were introduced to CTX-M-14 by site-directed muta-genesis based on overlap extension PCR with the former recombinant plasmid as PCR template, respectively.The wild-type CTX-M-14, recombinant CTX-M-14 protein and its variants were expressed and purified, then their steady-state kinetic parameters (Kcat, Km and Kcat/Km ) against β-lactam antibiotics were determined.Results The kinetic parameters of wild-type and recombinant CTX-M-14 had no statistically significant differences (P>0.1). The 1/Km, Kcat and Kcat/Km values of P167S variant against ceftazidime were 16-fold, 2.87-fold and 43.6-fold higher than those of recombinant CTX-M-14, respectively, and the Kcat/Km value of P167S variant against penicillin, ampicillin, cefazolin, cefuroxime, ceftriaxone, cefotaxime decreased( < 0.05). Compared with the kinetic parameters of recombinant CTX-M-14, the kinetic parameters of P167T variant against ceftazidime had no significant change, but the Kcat values of P167Q and P167G variants decreased dramatically(P<0.01). Conclusion There was no difference between the enzyme activities of wild-type and recombinant CTX-M-14. P167S variant could not only promote the enzyme affinity of CTX-M-14 to ceftazidime but also improve the conversion rate of enzyme-substrate complex in the ceftazidime hydrolysis. The comparison of the kinetic parameters of CTX-M-14 and its variants with Pro167 residue substitution showed that the increased activity of CTX-M ESBL variants against ceftazidime could not be simply explained with the enlarged cavity in active site that may be caused by the replacement of Pro167 residue by smaller amino acids.

Though Runx2 and Osterix are both key transcription factors in the pathway of osteoblast differentiation, whether Runx2 positively regulates Osterix being unknown. It was showed that Runx2 induced the gene expression of Osterix both in the non-osteoblastic cell lines, either pluripotent or differentiated, and in the osteoblastic cell lines. At the same time, the results also indicated that Runx2 up-regulated the activity of the 3.2 kb human Osterix promoter. Further experiments identified a highly conserved and functional Runx2 binding site "AGTGGTT" within the promoter. Thus the results support the hypothesis that Runx2 is involved in the regulation of the Osterix gene expression. Moreover, the transient transfection and dual-luciferase assay showed Osterix up-regulated the activity of the 2.3 kb type Ⅰ collagen promoter in the non-osteoblastic cells, but Runx2 did not. This difference implies that Osterix, the down stream transcription factor of Runx2 during osteoblast differentiation, is needed to stimulate the osteoblast-specific gene expression of type Ⅰ collagen.