Humans ; Occupational Diseases ; prevention & control ; Occupational Health Services ; organization & administration ; Risk Assessment ; Workplace

Currently,the diagnosis of malignant pheochromocytoma still faces a big challenge.Neither biochemical nor histological features can be reliably used to distinguish malignancy from benign tumors.Numerous efforts have been applied to identify novel molecular markers to predict malignancy.

China ; Occupational Exposure ; prevention & control ; Risk Assessment ; Workplace

China ; epidemiology ; Female ; Hepatitis B ; prevention & control ; transmission ; Humans ; Infectious Disease Transmission, Vertical ; prevention & control ; Pregnancy ; Pregnancy Complications, Infectious

The key point of development of emergency medicine is professional training. What we need are "emergency physicians who have well-knit theory and comprehensive technique" but "the internal physicians who could do some resuscitation". This is the goal of emergency medical education. To realize the goal, we need to improve our condition of training-bases, the curriculum,the academic level of trainer and training method. Besides, we should balance the effectivness and safety of training. We believe that the "four steps" course should be the most important and most effective method of emergency medical training.

Objective To approach CT features of brain in premature infants.Methods 30 premature infants were examined by helical CT with low dose.The morphology of brain was observed and the density of brain tissue was also measured.Results Premature infants had thin cerebral cortex and shallow sulci.The density of white matter showed lower CT density,the average CT value was(14.36?1.91) HU and CT value of the white matter tended to increase with fetal age.7 cases(fetal age

Objective The normothermic isolated contracting rat heart model was used to investigate the mechanism of protective effect of propofol on left ventricular function and myocardial metabolism against ischemia-reperfusion injury by determination of the catecholamme concentration in the coronary outflow. Methods forty healthy male SD rats weighing 310-450 g were randomly divided into 4 groups of each 10 animals: control group, propofol 10?mol/L (P10), 50?mol/L (P50) and 100?mol/L (P100). The animal were sacrificed by knock-out and the heart was immediately removed. The aorta was connected to a Larigendorff apparatus and retrogradely perfused with oxygenated (95% O, and 5% CO2) Krebs-Henseleit buffer (KHB) for 5 min. Then the left ventricle was perfused through a cannula inserted in pulmonary vein at a constant pressure of 12.5 cm H2O (preload). The pressure at aorta outflow was 90 cm H2O (afterload). Different concentrations of propofol in KHB were prepared. Global ischemia of the heart was induced by suspension of perfusion for 25 min followed by 30 min reperfusion. Coronary flow (CF), aortic flow (AF), cardiac output (CO = AF + CF), HR, left ventricular peak systolic pressure (LVPSP), left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP = LVPSP-LVEDP) and the product of LVDP and HR were measured and recorded 5 min and immediately before ischemia and 5, 10, 15, 20, 25 and 30 min following reperfusion. Coronary outflow was collected for determination of creatine kinase (CK) and catecholamine ( epinephrine, norepinephrine and dopamine) concentrations.Results Before ischemia CF was significantly higher and CO, HR, LVPSP and LVDP ? HR were significantly lower in the 3 propofol groups in comparison with the controls. During reperfusion CF, CO, HR, LVPSP and LVEDP recovered much better in the propofol groups than those in control group. In group P50 LVDP ? HR reached 88.7% of the pre-ischemic value while in control group only 56.3% . The CK and catecholamine concentrations were not significantly different among the 4 groups before ischemia. During reperfusion CK, epinephrine and noeepinepherine concentrations were significantly lower in propofol groups than in control group (P

Objective To investigate the effect of different target plasma concentrations (Cp) of remifentanil on sedative effect of propofol. Methods Eighty adult ASA Ⅰ or Ⅱ patients aged 18-60 yr undergoing elective laparoscopic cholecystectomy were randomly assigned into 4 groups (n = 20 each). Anesthesia was induced with TCI of remifentanil (Cp = 2, 4 and 8 ng/ml in group Ⅱ-Ⅳ respectively) and propofol TCI (the initial Cp of propofol was 2 μg/ml in the 4 groups). Then the Cp of propofol increased by 0.5 μg/ml every 1 min until BIS value decreased to 50. BIS value and the Cp of propofol were recorded as the patient lost consciousness.The Cp and consumption of propofol were recorded when BIS value decreased to 50. Results BIS value was significantly increased, while the Cp of propofol was significantly decreased as the patient lost consciousness, and the Cp and consumption of propofol were significantly decreased when BIS value decreased to 50 in group Ⅲ - Ⅳ compared with group Ⅰ (P ＜ 0.05). Conclusion Remifentanil 4 ng/ml is the suitable Cp for anesthesia when combined with propofol.

Purpose:To establish a human large cell lung cancer multi-drug resistance cell line H460/cDDP and explore its biological characteristics. Methods:A resistant human larget cell lung cancer cell line (H460/cDDP) was established by intermittent high dose cisplatin selection from the parental cell line H460. Drug sensitivity was detected by MTT assay. The changes of its biological characteristics were determined using light microscopy, Trypan Blue staining rejection, cell counting, chromatosome analysis; Neoplasia formation test in nude mouse was performed to investigate its in vivo characteristic. Results:H460/cDDP cell line was developed after about 6 months and the resistance index to cisplatin was 10.21. H460/cDDP cells exhibited cross-resistance to 5-Fuorouracil, adriamycn, etoposide and methotrexate. Compared with the parent cells, the morphology wac changed; doubling time prolonged (from 20.78h to 36.46h), while the chromatosome number and caryotype were similar. After being frozen, deposited and resuscitated repeatedly, its biological characteristics remained stable. It had neoplasia formation ability in vivo, but the forming time was longer than its parental cell. Conclusions:The newly established multi-drug resistant large cell lung cancer cell line H460/cDDP cell line possessed the typical multi-drug resistant phenotype. It was stable and had neoplasia formation ability in vivo,suitable for research.

Objective To compare the efficacy in patients with severe pancreatitis treated by minimally invasive surgery and open surgery. Methods Seventy-three cases of patients with severe pancreatitis which were treated in our hospital between June 2011 and June 2015 were divided into group A (n = 37) and group B(n =36). Group A were treated by minimally invasive surgery while group B by open surgery. The perioperative stress reactions , postoperative inflammatory reactions , incidence of complications and mortality were compared between the two groups. Results The proportion of patients with perioperative (0+1) level pain in group A (70.3%) was significantly higher than that in group B (44.4%) (P < 0.05). Seventy-two hours after surgery, the CRP, TNF-α and IL-6 in group A were lower than those before surgery and those in group B while APN in group A was higher than that before surgery and that in group B (P < 0.05). The incidence of pulmonary infection and mortality in group A (27.0%, 5.4%) were lower than those in group B (50.0%, 22.2%) (P < 0.05). Conclusions Minimally invasive surgery in the treatment of severe pancreatitis has slight trauma. The postoperative pain is mild and complications are fewer , and the mortality is low.