Acute Disease ; Humans ; Respiratory Tract Infections ; diagnosis ; etiology ; microbiology ; virology

Animals ; Arteritis ; complications ; immunology ; Coronary Artery Disease ; etiology ; pathology ; Coronary Vessels ; pathology ; Models, Animal ; Rabbits

OBJECTIVE:To observe clinical efficacy and safety of linezolid,teicoplanin and vancomycin in the treatment of hospital-acquired pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA). METHODS:120 patients diagnosed as hospital-acquired MRSA pneumonia were divided into linezolid group,teicoplanin group and vancomycin group according to therapeutic regimen,with 40 cases in each group. Linezolid group received Linezolid injection 600 mg,ivgtt,bid;teicoplanin group received Teicoplanin injection 0.4 g,ivgtt,bid;vancomycin group received Vancomycin injection 1 000 mg,bid,ivgtt. 3 groups received 2 weeks of treatment. Clinical efficacy and bacterial clearance effective rate of 3 groups were observed as well as serum levels of inflammatory factors before and after treatment. ADR of 3 groups were compared. RESULTS:The clinical effective rates of linezolid group,teicoplanin group and vancomycin group were 90.0%,72.5% and 67.5%;the effective bacterial clearance rates were 85.0%,60.0% and 57.5%,respectively. The clinical effective rate and the effective bacterial clearance rate in linezolid group were significantly higher than those in teicoplanin group and vancomycin group,with statistical significance (P<0.05). There was no statistical significance in above indexes between teicoplanin group and vancomycin group (P>0.05). There was no statistical significance serum inflammatory factors among 3 groups before treatment (P>0.05). CRP and PCT of 3 groups de-creased significantly after treatment,and those of linezolid group were lower than teicoplanin group and vancomycin group,with statistical significance(P<0.05). There was no statistical significance in serum inflammatory factors between teicoplanin group and vancomycin group before and after treatment (P>0.05). There was no statistical significance in the incidence of ADR among 3 groups (P>0.05). CONCLUSIONS:For hospital-acquired MRSA pneumonia,linezolid is better than teicoplanin and vancomycin in pneumonia control with good safety.

This review largely deals with the peptide toxins elaborated by marine cone snails of the genus Conus . Each species of Conus contains in its venom 50 to 200 different peptides directed at different macromolecular targts. These include competitive antagonists of postsynaptic nicotinic receptors (a-conotoxins), blockers selective for Na+ channels in skeletal muscle (u- conotoxins), blockers of presynaptic of antagonists of postsynaptic Ca2+ channels (w-conotoxins), activators of Na+ channels (s-conotoxins), blockers of K+ channels (k-conotoxins), blockers of nicotinic receptor channels (u-conotoxins) and antagonists of NMDA receptors (cono-sleeper).The small size of the peptides (13 to 30 residues is typical) has facilitated synthesis of many of them. A very attractive feature is the highly cross-linked conserved 2 to 3 disulfide bonds which make conotoxins conformationally rigid, some of conotoxins, however, are stabilized by r-carboxyglutamates. The Structure-Activity Relationships of conotoxins and a brief perspective have been reviewed in the paper.

Objective To discuss the utilization of signs of a proper depth of ablation during transurethral electrovaporization of the prostate (TUVP). Methods A retrospective analysis was made on clinical records of 616 cases of benign prostatic hyperplasia (BPH). Preoperative color ultrasonography revealed a co-morbidity of prostatic calcification in 310 cases. A total of 156 cases also had vesical calculi and 23 cases complicated tumors of the bladder. On the performance of TUVP, once a co-morbidity of prostatic calculus or a “slipping phenomenon” during mechanical operation was observed, the ablation was stopped. Results The operative time was 30~120 min (mean, 75 min). During the surgery, preoperatively diagnosed prostatic calcification in the 310 cases was confirmed as prostatic calculus, and in the remaining 306 cases prostatic calculus was also found. Vesical perforation occurred during the surgery in 1 case. No urethrorectal fistula or transurethral resection of the prostate syndrome happened. Follow-up observations were conducted for 3~6 months (mean, 4.5 months) in 325 cases. The maximal flow rate (Qmax) was elevated from 6.5~8.5 ml/s to 8~22 ml/s. The residual urine volume (RU) decreased from 70~150 ml to 0~10 ml, and the International Prostate Symptom Scores (I-PSS), from 19~24.5 to 0~7. Of 138 cases of vesical calculus and 23 cases of bladder tumor, no recurrence was noted. Conclusions Prostatic calculus and the “slipping phenomenon” indicate a proper depth of ablation during transurethral electrovaporization of the prostate.

Objective To study the molecular biology of rifampin-depending M. Tuberculosis. Methods The seguence (a 319-bp DNA fragment) of rpoB gene were analyzed by automated DNA sequencing machine. (2) The fingerprints of genomic DNA were obtained by random amplified polymorphic DNA (RAPD) fingerprinting. (3)The protein electrophoresis of bacterium by SDS-polyacrylamide gel (SDS-PAG).(4) The cases of pulmonary tuberculosis by rifampin-depending strains were retrospectively analyzed. Results (1) rpoB gene sequenced: The point mutationrate of rifampin-depending strainswas 96.7%(29/30) and that of rifampin-residtant strains 81.1%(30/37), P

OBJECTIVE To investigate the effect of furanodiene(FDE),a diterpene derived from the medicinal plant Zedoary,on apoptosis of human gastric cancer MGC-803 cells induced in vitro. METHODS MGC-803 cells were treated with FDE 46.29~740.74μmol·L-1 for 24,48 and 72 h,and the cell viability was detected with MTT assay. Cell morphology was observed by light microscopy and Hoechst33342 staining. Flow cytometry was used to detect cell apoptotic rate and cell cycle. Rh123 staining and fluorescence probe DCFH-DA were employed to detect the changes in mitochondrial membrane potential (MMP) and reactive oxygen species(ROS). RESULTS MTT Results showed that FDE 46.29-740.74μmol · L-1 exhibited significantly higher cytotoxicity to gastric cancer MGC-803 cells. IC50 for MGC-803 of 24,48 and 72 h treatment was 347.91,257.41 and 101.01μmol·L-1,respectively. Treatment with FDE 92.58-370.32μmol·L-1 for 24 h also caused significant morphological changes in MGC-803 cells. AnnexinⅤ-FITC/PI double staining showed that the apoptotic rate increased after FDE 92.58-370.32μmol·L-1 treatment for 24 h(P<0.05). FDE enabled MGC-803 cell cycle arrest in S phase. DCFH-DA staining showed that FDE resulted in an increase in intracellular ROS levels(P<0.05) when PDE concentration was 370.37μmol·L-1(P<0.05). MMP decreased after FDE treatment when PDE concen?tration was 370.37μmol·L-1(P<0.05). CONCLUSION FDE Possesses potent tumor selected toxicity and can induce apoptosis of MGC-803 cells through cell cycle arresting,which is related to inhibition of DNA biosynthesis.

BACKGROUND:Percutaneous kyphoplasty for the treatment of vertebral compression fractures has gained good clinical results and it is characterized as smal trauma, less bleeding and very low rate of complications. The vast majority of elderly patients can tolerate it, but this method cannot prevent fracture replase in the elderly. OBJECTIVE:To evaluate clinical outcomes of percutaneous kyphoplasty combined with anti-osteoporosis drug for the treatment of acute osteoporotic vertebral compression fractures METHODS:According to strict inclusion and exclusion criteria, 137 patients, including 26 males and 111 females, mean age of (75.55±6.96) years, with a total of 198 acute osteoporotic vertebral compression fractures treated by kyphoplasty that involves injection of polymethyl methacrylate cement under radiologic control into a treated vertebral body were conducted in this study. Al patients were asked to take anti-osteoporosis drugs for 3 post-treatment months. The primary outcomes were visual analogue scale, ertebral restoring rate, Oswestry disability index, Cobb angle at different time (pre-operation, 1 week and 3 months after operation). In addition, the rate of complications and the replase rate of vertebral compression fractures after operation were recorded. RESULTS AND CONCLUSION:There were significant differences in the mean visual analog scale scores, vertebral restoring rate, Oswestry disability index, Cobb angle at pre-procedure and post-procedure (at 1 week and 3 months) (P<0.001). In addition, the rate of postoperative complications was 0.7%and there were no vertebral compression fractures during 3-month fol ow-up period. Our study suggests that percutaneous kyphoplasty combined with anti-osteoporosis drug for the treatment of acute osteoporotic vertebral compression fractures can gain good clinical results.

A non spore fungal strain designated as CR 9512 was isolated from a species of Cordyceps , found in Northern Guangdong of China The growth characteristics and the morphology of the organism were studied It was initially identified as Rhizoctonia sp ,Agonomycetales Its antimicrobial tests, against 26 strains of Gram positive and Gram negative bacteria, actinomycetes, filamentous fungi and yeast, showed strong inhibition to some yeast of Rhodotorula, Saccharomyces, Pichia and Cryptococcus , and weak inhibition to yeast of Candida The speciality on a limited spectrum anti yeast of the organism will show an enormous value for researches and applications

Objective: To evaluate the protective effect of epidermal growth factor (EGF) on intestinal barrier functionin rats with acute pancreatitis. Methods: Thirty-two male SD rats received injection of sodium taurocholate solution(3. 5 mg?L-1) into the pancreatic duct were randomly divided into control group (n=16) and treatment group (n=16). Animals incontrol group received total parenteral nutrition (TPN), animals in treatment group were fed on the same TPN formula ascontrol group and injections of EGF at a dose of 0. 2 mg' kg l' day--'. Rats were sacrificed on d 1 and d 5 of TPN. Concen-tration of xylose and fluorescein isothiocyanate (FITC)-dextran in superior mesenteric vein (SMV), protein and DNA contentin je junal mucosa were determined. Samples from SMV, mesenteric lymph nodes, pancreas, liver, spleen were harvested forcultures. Results: FITC-dextran concentration in treatment group was significantly lower than in control group [(3. 4?0. 7)vs (7. 5?0. 9) mg. L-1, P＜0. 0l]. Protein and DNA content in je junal mucosa in treatment group were significantly higherthan in control group [(2. 65?0. 23) vs (1. 12?0. 18) mg? cm-1, (0. 25?0. 07) vs (0. 12?0. 04) mg?cm-1, P