1.Gene Diagnosis of Spinal Muscular Atrophy in Children
guo-an, LIANG ; bo-lin, ZHOU ; zhong-sheng, YU
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To establish a gene diagnosis assay for spinal muscular atrophy(SMA) in children. Method Analysis of the survival motor neuron (SMN) gene in 19 SMA patients and in 21 normal controls were performed by using polymerase chain reaction - fragment length polymorphism (PCR - RFLP) method. Result Deletion of exon 7and 8 in SMNt gene were found in all 19 SMA patients, while no such changes were found in normal controls. Conclusion The SMNt gene exon 7 and 8 examine can be applied to SMA gene diagnosis, and the PCR- RFLP method have higher sensitivity and particularity to the SMA diagnosis.
2.Correlative studies on uPAR receptor mRNA expressions with vascular endothelial growth factor, microvessel density, progression and survival time of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA ; Wen-juan XU ; Zhong MENG
Chinese Journal of Pathology 2005;34(5):306-307
Adenocarcinoma
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blood supply
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metabolism
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mortality
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secondary
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Adult
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Aged
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Female
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Humans
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Lymphatic Metastasis
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Male
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Microcirculation
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Middle Aged
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Neovascularization, Pathologic
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metabolism
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pathology
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Prognosis
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RNA, Messenger
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biosynthesis
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genetics
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Receptors, Cell Surface
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biosynthesis
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genetics
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Receptors, Urokinase Plasminogen Activator
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Stomach Neoplasms
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blood supply
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metabolism
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mortality
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pathology
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Survival Rate
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Vascular Endothelial Growth Factor A
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metabolism
4.A study of integrin beta3 mRNA expression and its correlation with microvascular density, progression and prognosis of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA
Chinese Journal of Pathology 2004;33(1):16-20
OBJECTIVETo investigate mRNA expression of integrin beta3 in gastric carcinoma, its correlation with microvascular density (MVD), growth-pattern, invasion, metastasis and prognosis.
METHODSIn situ hybridization and immunohistochemistry techniques were used to study the expression of integrin beta3 mRNA and CD34 protein in 105 gastric carcinoma specimens.
RESULTSIn situ hybridization revealed a 30% (6/20) positive rate of integrin beta3 mRNA in non-tumor gastric mucosa, which was significantly lower than that of the gastric cancer group (61.0%, 64/105, chi(2) = 8.85, P = 0.037); In infiltrating type cases (70.2%, 40/57), stage III - IV (72.1%, 44/61), lymphatic metastasis (68.6%, 48/70) and distant metastasis (85.7%, 36/42), the positive expression rates were significantly higher than those of the expanding type (chi(2) = 14.97, P = 0.002), stage I - II (chi(2) = 15.21, P = 0.015), non-lymphatic metastasis (chi(2) = 17.89, P = 0.025) and non-distant metastasis (chi(2) = 20.22, P = 0.005; chi(2) = 21.35, P = 0.035); its mean MVD was also significantly higher than that of the expanding type (t = 10.105, P = 0.001), stage I approximately II (t = 5.961, P = 0.001), non-lymphatic metastasis (t = 3.819, P = 0.01)and non-distant metastasis (t = 10.578, P = 0.001; t = 7.882, P = 0.001), respectively; The mean MVD (41.02 +/- 8.55)/0.72 mm(2) of the integrin beta3 mRNA positive expression group was significantly higher than (25.26 +/- 11.25)/0.72 mm(2) of the negative expression group. There was a positive relation between MVD and integrin beta3 mRNA expression in the tumor (r(s) = 0.316, P = 0.001). The mean survival time in cases with positive integrin beta3 mRNA expression or MVD value >or= 39.5 was significantly shorter than that in cases with negative expression or MVD value < 39.5.
CONCLUSIONSIntegrin beta3 expression correlates with enhanced tumor angiogenesis and may play a role in the invasion and nodal metastasis of gastric carcinoma, therefore it may serve as a prognostic marker of gastric cancer.
Adult ; Aged ; Disease Progression ; Female ; Humans ; Integrin beta3 ; genetics ; Male ; Middle Aged ; Neovascularization, Pathologic ; etiology ; Prognosis ; RNA, Messenger ; analysis ; Stomach Neoplasms ; blood supply ; metabolism ; pathology ; Survival Rate
5.Effects of ketamine on hypoxic-ischemic brain damage in newborn rats.
Shu-ling ZHANG ; Quan-zhong CHANG ; Yin-sheng LI ; Zhibin QIAN ; Dongxia LZ ; Xueping GUO
Chinese Journal of Pediatrics 2004;42(5):387-388
Animals
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Animals, Newborn
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Brain
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drug effects
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metabolism
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pathology
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Disease Models, Animal
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Excitatory Amino Acid Antagonists
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pharmacology
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therapeutic use
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Female
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Hypoxia-Ischemia, Brain
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drug therapy
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metabolism
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Ketamine
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pharmacology
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therapeutic use
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Male
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Treatment Outcome
6.Comparison of the clinical characteristics of ovarian lymphoma and ovarian cancer with bulk lymph node involvement
Feifei GAO ; Lei GUO ; Kai XUE ; Weiqi SHENG ; Wei WANG ; Mujie LI ; Zhong ZHENG ; Ziting LI
China Oncology 2017;27(4):281-286
Background and purpose: Ovarian lymphoma (OL) is usually misdiagnosed as ovarian cancer with bulk lymph node invasion (OC-BLN), and vice versa. Therefore, to distinguish these two types of disease, we compared their clinical characteristics in this study. Methods: This study retrospectively reviewed 14 OL and 14 OC-BLN patients from Fudan University Shanghai Cancer Center and Shanghai Eighth People's Hospital. The clinical char-acteristics, image and laboratory examination data were compared. Results: There was no significant difference in age, symptom, fever, weight loss and volume of ascitic fluid between the two groups. Comparing with OC-BLN, OL patients have larger tumor in ovaries [(13.04±5.94) cm vs (7.78±6.38) cm, P=0.033], and higher percentage of solid ovarian tumor (85.71% vs 28.5%, P=0.006). Lactate dehydrogenase(LDH)/CA125 was higher in OL (7.66±8.03) than OC-BLN (0.31±0.27, P=0.009). Using LDH/CA125 to diagnose OL, area under the curve (AUC) was 0.952. When the threshold value was set at 1, the sensitivity and specificity was 91.7% and 100%, respectively. Conclusion: OL and OC-BLN are easily to be misdiagnosed. OL has larger and more solid tumor than OC-BLN. LDH/CA125 can help to distinguish these two diseases and guide clinical decision making.
8.Overexpression and RNAi vectors built for key secondary metabolic pathway genes PAL, HMGR, PGT of Arnebia euchroma.
Teng XIE ; Yu-Zhong LIU ; Sheng WANG ; Tan LIU ; Li-Ping KANG ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2014;39(23):4538-4543
Arnebia euchroma is the main source for medicinal herb Zicao. and its most important component shikonin compounds have high medicinal and industrial value. This research is aimed to build overexpression vectors and RNAi vectors for key secondary metabolism genes of A. euchroma, and bulid platform for constructions of related transgenic lines using GATEWAY technology. To build genetic material based genetic research platform is to provide a great convenience for digging and functional verification of the genes on secondary metabolic pathway, and also to fill the gaps in transgenic research of A. euchroma. This study is also important for the cultivation of shikonin high-yielding strains of A. euchroma.
Boraginaceae
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genetics
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metabolism
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Genetic Vectors
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genetics
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metabolism
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Plant Proteins
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genetics
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metabolism
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RNA Interference
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Secondary Metabolism
9.Expression of obese receptor in oropharynx tissue in obstructive sleep apnea-hypopnea syndrome.
Sheng-guo WANG ; Ben-zhong ZHOU ; Jing-wu SUN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(10):787-788
Adult
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Female
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Humans
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Male
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Middle Aged
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Pharynx
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metabolism
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Receptors, Leptin
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metabolism
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Sleep Apnea, Obstructive
;
metabolism
10.Clinical analysis of primary percutaneous coronary intervention in patients with acute myocardial infarction
Guo-Zhong YU ; Qing-Lian LU ; Yan-Sheng GE ; Ji-Han CHEN ; Hong-Xi CHEN ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(08):-
Objective To report the clinical effect of primary percutaneous coronary intervention(PCI)in patients with acute myocardial infarction(AMI).Methods A retrospective study was accomplished on the clinical data of 13 AMI patients who underwent PCI from March 2004 to April 2006.Results The infarct-related artery (IRA)was successfully recanalized by primary PCI for 12 AMI patients,without major complications occurred in these cases during hospitalization.Conclusion Primary PCI should be firstly chosen for treatment of AMI in the hospitals which could carry out PCI.