Carcinoma, Squamous Cell ; radiotherapy ; Head and Neck Neoplasms ; radiotherapy ; Humans

ObjectiveTo evaluate a second endoscopy for prevention of delayed bleeding after ESD.MethodsData of 67 patients with gastric epithelial neoplasms undergoing ESD from May to November 2011 were reviewed.The median age was 63 ( 31 ～ 84) years.All patients were followed up by endoscopy on the first and the third day after ESD.ResultsOf 67 lesions,5 were located at cardia,6 at gastric body,3 at fundus,35 at antrum,16 at gastric angle,and 2 at residual stomach.The mean maximum diameter of the lesions was 3.73±4 1.24 (2.0 ～ 7.0) cm.There were no intraoperative complications.Post-ESD delayed bleeding was detected by endoscopy in 6 (9.0％ ) patients,with 5 on the third day and 1 on the fourth day.Forrest grading showed 2 cases of Ⅰ b,and 4 of Ⅱ b.All 6 cases were cured by endoscopy.The incidence of postoperative bleeding was far more than that evaluated based on the patients' clinical manifestations only.But therapeutic effect and saffety were the same according to the follow-up results.ConclusionIncidence of post-ESD bleeding is high,but there are no symptoms or severe consequences,so a second endoscopy after gastric ESD may contribute little to the prevention of delayed bleeding.

Objective The role of long non-coding RNA Linc00467 in human lung adenocarcinoma is not yet clear.This study was to investigate the expression of long non-coding RNA Linc00467 in human lung adenocarcinoma, its clinical significance, and the effects of Linc00467 on the functions of the tumor and endothelial cells in vitro.Methods Lung adenocarcinoma tissue and normal tissue surrounding the malignance were obtained from 60 patients with pathologically proved stage I-Ⅲa lung adenocarcinoma.Human umbilical vein endothelial cells (HUVECs) were transfected with the over-expressed plasmid pccl-Linc00467 (HUVEC experimental group) or the empty vector pccl (HUVEC control group), A549 cells with Linc00467-siRNA (A549 experimental group) or negative siRNA (A549 control group), and H1299 cells, too, with Linc00467-siRNA (H1299 experimental group) or negative siRNA (H1299 control group).The expression level of Linc00467 in the lung adenocarcinoma tissue was detected by qRT-PCR with an analysis of its correlation with the clinicopathological characteristics of the patients;the influence of Linc00467 on the proliferation of the A549, H1299 and HUVEC cells was assayed with CCK-8;and the role of Linc00467 in the angiogenesis of the HUVECs was assessed by fibrin bead sprouting assay.Results The expression of Linc00467 in the lung adenocarcinoma tissue was 2.72±1.31 times as high as that in the normal lung tissue (P<0.01), and those in the A549 and H1299 cells were 3.45±0.25 and 3.22±0.33 times as high as those in the human bronchial epithelial (HBE) cells (P<0.01).The expression level of Linc00467 was significantly correlated with the tumor size and vascular invasion (P<0.05).After transfection of Linc00467-siRNA, the expressions of Linc00467 in the A549 and H1299 experimental groups were down-regulated by 72% and 68% as compared with those in the A549 and H1299 control groups (P<0.01).The number of living cells was remarkably decreased in the A549 experimental group in comparison with the A549 control at 48 h (1.29±0.07 vs 1.51±0.09), 72 h (1.53±0.15 vs 2.13±0.11), and 96 h after culturing (1.98±0.18 vs 3.02±0.12), and so was it in the H1299 experimental versus the H1299 control group, but markedly increased in the HUVEC experimental versus the HUVEC control group (P<0.05).At 5 days, HUVEC experimental group, as compared with the HUVEC control, showed a significantly increased number of newly formed vascular branches (7.36 vs 4.25/superbead, P<0.01) and relative length of the blood vessels (3.12 vs 1, P<0.01).Conclusion Linc00467 promotes tumor cell proliferation and angiogenesis and is highly expressed in the lung adenocarcinoma tissue, which is correlated with the tumor size and vascular invasion and suggests that Linc00467 could be a potential biomarker and therapeutic target.

This paper discusses variations of laparoscopic transgastric cystogastrostomy in management of retrogastric pancreatic pseudocysts for 8 patients with symptom or pseudocysts (larger than 6 cm) companied with clinical manifestations.Using a Harmonic scalpel,two 3-5-cm incisions were made in the anterior and posterior gastric wall respectively.In the last step,the anterior gastrotomy was closed with an Endo-GIA stapler.All cases were successfully treated without large blood loss and without conversion to open surgery.The mean operative time was 114.29±19.24 min,blood loss was 157.14±78.70 mL,and mean hospital stay was 8.29±2.98 days,Gastric fistula occurred in one case on the postoperative day 7,and closed 1 month later.No bleeding was seen in all patients during the perioperative follow-up period.CT scans,given one month after the surgeries,displayed that the pancreatic pseudocysts disappeared or decreased in size,and ultrasounds showed no fluid or food residue in stomas at the third and fifth month following surgery.No patient experienced a recurrence during the follow-up period.Transgastric laparoscopic cystogastrostomy is a minimally invasive surgical procedure with a high rate of success and a low rate of recurrence,accompanied by rapid recovery.It is easy to master,safe to perform and may be the preferred option to treat retrogastric pancreatic pseudocysts.

Child, Preschool ; Chondromatosis, Synovial ; diagnosis ; diagnostic imaging ; surgery ; Diagnostic Errors ; Female ; Humans ; Tibial Fractures ; diagnosis ; Tomography, X-Ray Computed

OBJECTIVETo study the protective effects of garlic oil (GO) on the peripheral nerve injuries induced by n-hexane.

METHODSMale Wistar rats were randomly divided into four groups (10 rats in each group): the control, the n-hexane treatment (2000 mg/kg), the low dose GO, and the high dose GO groups. The rats in the low and high doses of GO groups were pretreated with GO (40 and 80 mg/kg) before exposure to n-hexane (2000 mg/ kg), while the animals of the n-hexane treatment group were given normal saline and then 2000 mg/ kg n-hexane. The rats were exposed to GO and n-hexane 6 times a week for 10 weeks. The gait scores and staying time on the rotating rod for all rats were detected every two weeks. The rats were sacrificed at the end of ten weeks, then the levels of alcohol dehydrogenase (ADH), maleic dialdehyde (MDA), reduced glutathione (GSH), glutathione peroxidase(GSH-Px), total antioxidation capacity(T-AOC) and the ability of inhibition of *OH in livers were examined.

RESULTSThe gait scores increased significantly and the time staying on the rotating rod obviously decreased in rats of n-hexane treatment group, as compared with control group (P < 0.05 or P < 0.01). In the hepatic tissues of n-hexane group, the levels of MDA and ADH significantly increased, the activities of GSH-Px, T-AOC and the ability of inhibition of *OH obviously decreased, as compared to control group (P < 0.05 or P < 0.01). In 2 GO groups, the gait scores and the staying time on the rotating rod were significantly improved, the levels of MDA and ADH significantly decreased, the activities of GSH-Px, T-AOC and the ability of inhibition of *OH obviously increased, as compared with n-hexane group (P < 0.05 or P < 0.01 ).

CONCLUSIONADH could play an important role in the protective effects induced by garlic oil on the peripheral nerve injuries produced by n-hexane.

Alcohol Dehydrogenase ; metabolism ; Animals ; Garlic ; Hexanes ; toxicity ; Lipid Peroxidation ; drug effects ; Liver ; enzymology ; metabolism ; Male ; Neuroprotective Agents ; pharmacology ; Peripheral Nerve Injuries ; chemically induced ; metabolism ; Plant Oils ; pharmacology ; Rats ; Rats, Wistar

Aim To study the apoptosis effect of cucurbitacin Ⅱa on non-small cell lung cancer cell lines NCI-H460 and A549 and its underlying mechanism.Methods Cell viability was assessed by CCK-8 assay.The apoptosis effect and cell cycle arrest were detected by Flow cytometry.Western blot was employed to detect the related protein.Results The proliferation of lung cancer cell lines NCI-H460 and A549 was inhibited by CuⅡa, which showed cytotoxic activity with IC50 values of 224.9 nmol·L-1 and 108.3 nmol·L-1 against NCI-H460 and A549 respectively.CuⅡa induced the cells apoptosis and cell cycle arrest at G2/M phase.The results of Western blot showed CuⅡa inhibited the phosphorylation of STAT3 and Cofilin in a dose-dependent manner.Further, CuⅡa inhibited the phosphorylation of Aurora A, in line with the important characteristics of anti-tumor effect of Aurora A kinase inhibitor with blocking cells in the G2/M phase.Conclusion CuⅡa has obvious anti-tumor effect against non-small cell lung cancer, which suggests its value as a lead compound for lung cell carcinoma.

Objective This study was to estimate the prevalence of elderly mistreatment (EM) in rural community and to examine the association between social support and the risk of the EM.Methods A cross-sectional descriptive study was performed in three rural communities (17 villages) in Macheng city of Hubei province.2000 subjects aged 60 years or older were selected using cluster sampling.Questionnaire being developed would include general information,a scale to measure social support,and a modified vulnerability on abuse screening scale (VASS) to measure the EM.Results The prevalence rates of EM,physical abuse,emotional abuse,neglect and financial exploitation for rural elderly people in Macheng city were 36.2％,4.9％,27.3％,15.8％,and 2.0％respectively.After adjusting for potential confounding factors,necessary practical support from family (OR=1.28,95％CI:1.01-1.63) was the risk factor causing EM while having got practical support (OR=0.76,95％CI:0.58-0.98) or moral support (OR=0.63,95％CI:0.49-0.82) from family and moral support from friends (OR=0.73,95％CI:0.59-0.90) when in need were the protective factors.The protective factors on physical abuse,emotional abuse,neglect and financial exploitation would include:getting practical support from family when in need (OR=0.59,95％CI:0.35-0.99),getting moral support from family (OR=0.67,95％ CI:0.51-0.89) and friends (OR=0.67,95％ CI:0.54-0.84) and getting practical support from family when in need (OR=0.63,95％CI:0.45-0.88),getting practical support from family ( OR =0.38,95％ CI:0.14-0.98 ) and getting moral support from friends when in need (OR=0.42,95％CI:0.20-0.87),respectively.Conclusion High prevalence of EM was seen in the rural areas of Macheng city.Social support was an important protective factor for EM in this population.

Objective To compare with the therapy effect of bevacizumab or bevacizumab combined with dose-dense temozolomide on recurrent malignant gliomas.Methods Thirty-eight patients with recurrent malignant glioma,admitted to our hospital from June 2008 to June 2012,were chosen in our study.Among them,26 patients accepted bevacizumab therapy (single drug group) and 12 accepted bevacizumab combined with dose-dense temozolomide therapy (unite drugs group).Then,the clinical data,progressive free survival (PFS) and adverse effects were retrospectively analyzed.Results Single drug group:the median PFS was 21.8 weeks,PFS-6 months was 37.8％;united drug group:the median progressive free survival was 34.7 weeks,PFS-6 months was 52.7％;significant differences were noted between the two groups (x2=8.161,P=0.023) Conclusion Bevacizumab can prolong the PFS of patients with recurrent malignant glioma;the therapy effect of bevacizumab and dose-dense temozolomide is better than single drug,which can be recommended as an effective therapy for recurrent malignant glioma.

Objective To investigate the effects of the variables of superconducting knife diameter and freezing time of Endocare CryocareTM surgical system on the necrosis extent and micro-pathological changes of the normal frozen brain tissue of dogs. Methods Argon-helium refrigeration superconducting knives of 2 and 3mm in diameter were respectively inserted into the right frontal lobe of dog brains, and the brains were frozen for 3 and 5min, respectively. The procedure was repeated one time. 48h later, the brains were perfused and harvested for the pathological observation under light microscope and electron microscope and the determination of necrosis area. Results 48h after refrigeration, the frozen brains presented hemorrhagic necrosis and were obviously divided into the central necrosis, inflammatory reaction zone, bleeding and edema zone. The boundaries were clear. Survival of brain cell structure was not found in the edge of frozen area. Conclusion Two freeze-thaw cycles with the freezing time of 3 and 5min are enough to cause the death of frozen brain tissue.