Objective To summarize the perioperative nursing of patients with post-thoracotomy atelectasis treated with bronchoalveolar lavage combined with pulmonary insufflation.Methods Before operation,the nursing measures included the full preoperative preparation,full anesthesia and mental care.During the operation,the nursing focused on intensified monitoring,close cooperation with doctors in manipulation of bronchoalveolar lavage and pulmonary insufflation.After operation,the postoperative nursing was performed.Results The lung recruitments were successful in all the 12 patients.Compared to pre-operation,the arterial oxygen saturation(SaO2)and arterial oxygen partial pressure(PaO2)6 hours after treatment were significantly improved(P<0.05) while the CO2 partial pressure(PaCO2)was significantly decreased(P<0.05).There were no severe complications and no recurrence of pulmonary atelectasis.All the patients were successfully discharged.Conclusions The bronchoalveolar lavage combined with pulmonary insufflation is effective in treating post-thoracotomy atelectasis.To ensure the smooth treatment,perioperative nursing is also contributive.

Objective To observe the changes of specific proteins in serum of acute stage patients with SARS and its clinical application. Methods The acute stage patients with SARS group included 42 cases who had clinical symptoms less than five days. In normal group: 30 healthy adults were as controls. Tests for specific proteins were immunoglobin G ( IgG ) , immunoglobin A ( IgA) , immunoglobin M ( IgM) , complement C3( C3),complement C4(C4),C-reaction protein (CRP), al acid glycoprotein( a1-AG) , a1 antitrypsin(a1-AT) ,transferrin(TRF), ceruloplasmin( CER) in sera by Ratenephelometer. Results The level of CRP, a1-AG, a1-AT in sera of patients with SARS were higher than that in control group ( P 0. 05). Conclusion It suggested that specific proteins in serum of acute stage patients with SARS change in a particular way. There were some help for the diagnosis of the phlogistic degree of acute stage patients with SARS by detecting CRP, a1-AG and TRF simultaneously.

Objectives: To evaluate the safety and efficacy of CT-guided iodine 125 seed implantation combined with gemcitabine and 5-fluorouracil (5-FU)-based intra-arterial perfusion in the treatment of patients with advanced pancreatic cancer. Methods: A total of 16 patients with advanced unresectable pancreatic cancer underwent CT-guided iodine implantation combined with gemcitabine plus 5-FU-based intra-arterial perfusion. One week after implantation of iodine 125 seeds, all the patients received concurrent intra-arterial infusion of gemcitabine and 5-FU, which was repeated for 3 to 4 cycles. Three patients complicated with liver metastasis were given additional arterial embolization concurrently. Survival period, pain-relieving period, and adverse reaction of patients were observed. Results: All the patients were followed up for 2-25 months. CT scan found that there were 2 cases with complete response, 9 cases with partial response, 3 cases with stable disease, and 2 cases with progressive disease after 2 months of follow-up. The overall effective rate (CR + PR) was 68.8%. The median survival period for all enrolled patients was 11 months. It was 15 months for patients at stage II + III and 9 months for those at stage IV. No serious complications including gastrointestinal bleeding, pancreatitis, pancreatic fistula and radiation colitis were observed. Conclusion: CT-guided iodine 125 seed implantation combined with gemcitabine and 5-fluorouracil (5-FU)-based intra-arterial perfusion has better palliative pain-relieving effects. It has safe and effective effects in short terms and causes less complications in the treatment of advanced pancreatic cancer patients.

Objective: To investigate the expressions of P-glycoprotein (P-gp), topoisomerase II alpha (ToPo II α), glutathione-S-transferase-pi (GST-π), and oncogene c-erbB2 in endometrial cancer and their significance. Methods: Immunohistochemistry SP method was used to determine the expressions of P-gp, ToPo II α, GST-π and c-erbB2 in 127 tumor samples from patients with endometrial cancer. Results: Among 127 tumor samples from patients with endometrial cancer, the positive expression rates of P-gp, ToPo II α, GST-π and c-erbB2 were 63.0%, 44.9%, 70.1%, and 47.2%, respectively. The co-expression rate of P-gp and GST-π was 50.7%. The expression levels of P-gp, GST-π and c-erbB2 in poor differentiation endometrial cancer were significantly higher than those in well/moderate differentiation endometrial cancer (P < 0.05). While the expression level of ToPo II α in well/moderate differentiation endometrial cancer was significantly higher than that in poor differentiation endometrial cancer (P < 0.05). Univariate analysis indicated that the expression of P-gp, GST-π and c-erbB2 were associated with the survival time and poor prognosis of endometrial cancer patients. COX regression analysis suggested that the expression of P-gp and co-expression of P-gp and GST-π correlated with the survival rate of patients. Over-expression of ToPo II α tended to have protective effects on patients. Conclusion: Multidrug resistance-associated protein (MRP), GST-π, Topo II α, and lung resistance protein (LRP) plays important roles in the multidrug resistance(MDR) of tumor chemotherapy. Combined effects of the four multiple factors, P-gp, ToPo II α, GST-π and c-erbB2 were the main action mechanism for the MDR of endometrial cancer. It has an important guiding significance for predicting the prognosis and making rational chemotherapeutic regimens for patients with endometrial cancer.

A myxobacterial strain, So ce cpu-1, was isolated from a soil sample near the Huanghai beach. So ce cpu-1 had broad antimicrobial activity, active component has maxima] absorption at 210 nm. The effects of different culture conditions on the yield of the secondary metabolites were investigated. The results showed that, when cultivating the strain in the M, medium (containing 10% w/v D312 neutral absorber resin ), the air up to 70% of the whole flask volume, adding 5?L the secondary metabolites as the revulsant, at 30℃, 200r/min, for 6 days, the yield of the secondary metabolites achieved the maximum.

The animal models of hypoxic-ischemic brain injury have been established inmany animals, such as monkeys, dogs, rats, mice, rabbits, and pigs. These models have provideda great deal of important information for neonatal hypoxic-ischemic brain injury. Howerver, thedifferent species vary in their susceptibility to the various types of ischemic insults. This articlereviews the animal models of hypoxic-ischemic brain injury in different species.

Objective To study the molecular biology of rifampin-depending M. Tuberculosis. Methods The seguence (a 319-bp DNA fragment) of rpoB gene were analyzed by automated DNA sequencing machine. (2) The fingerprints of genomic DNA were obtained by random amplified polymorphic DNA (RAPD) fingerprinting. (3)The protein electrophoresis of bacterium by SDS-polyacrylamide gel (SDS-PAG).(4) The cases of pulmonary tuberculosis by rifampin-depending strains were retrospectively analyzed. Results (1) rpoB gene sequenced: The point mutationrate of rifampin-depending strainswas 96.7%(29/30) and that of rifampin-residtant strains 81.1%(30/37), P

Objective: To determine the inhibitory effects of maltitol-gum on Streptococcus mutans, Lactobacilli and Actinomyces viscosus in dental plaque. Methods: Thirty 13-15 years old children with DMFS>4 were divided into three groups, maltitol chewing gums group(A group), xylitol chewing gum group(B group) and blank gum base group (C group). The plaque samples were collected and colony forming units were counted. Results: The levels of three-species cariogenic pathogens in three groups were statistically down-regulated when compared with the baseline(P<0.001).Moreover, A group and B group resulted in a higher decrease of Streptococcus mutans levels compare with C group(P<0.05). The levels of Lactobacilli and Actinomyces viscosus were not statistically different between groups(P>0.05). Conclusion: Maltitol-gum can lead to a significant suppression on Streptococcus mutans levels in dental plaque,while the inhibitory effect of the maltitol-gum on Lactobacilli, Actinomyces viscosus is not obvious.

In order to identify host factors which interact with the movement protein (MP) of Apple chlorotic leaf spot virus (ACLSV), ACLSV MP was cloned into the bait vector pGBKT7 and used to screen a cDNA library of Malus sylvestris cv. R12740-7A, which had previously been constructed by yeast two-hybrid sequencing transformation. The protein functions of the identified host factors were determined according to their gene annotations in GenBank. The result showed that the bait plasmid pGBKT7-MP showed no virulence or self-activating effect on yeast strain Y2H Gold. Sixty-nine interactor proteins were identified, which were divided into the following 10 classes according to their described functions: hydrolases; pathogenesis-related proteins; DNA binding proteins; phosphatases; ligases; proteins with catalytic activity; phenylalanine ammonialyases; peroxidases; NAD binding proteins; and proteins of unknown function. Bioinformatic analysis of gene homology suggested that phosphatases, pathogenesis-related proteins and glyceraldehyde-3-phosphate dehydrogenase A may play an important role in the interaction between virus and host. This study may provide a theoretical basis for the further study of viral pathogenesis and virus-host interaction mechanisms.
Flexiviridae ; genetics ; metabolism ; Malus ; genetics ; metabolism ; virology ; Molecular Sequence Data ; Plant Diseases ; genetics ; virology ; Plant Proteins ; genetics ; metabolism ; Plant Viral Movement Proteins ; genetics ; metabolism ; Protein Binding ; Two-Hybrid System Techniques

Objective To investigate the effects of three external nasogastric tube fixations on unplanned nasogastric tube removal after radical esophagectomy for esophageal cancer.Methods Two hundred and thirty four esophageal cancer patients who underwent radical esophagectomy with nasogastric intubation were assigned into conventional group I(n=74),conventional group II (n=79)and experiment group(n=81)using random number table.For external fixation of the nasogastric tube,the traditional group I used“3M”silk tape,the traditional group II used a white flat strap with two knots and the experiment group used anI-shaped nasal fixation tape combined with a flapping cheek fixation tape.The three groups were compared in terms of displacement of the indwelling nasogastric tube and slippage of the tube.Results The displacement rate of nasogastric tube and the rate of slippage in the experiment group were both significantly lower than those in both conventional groups I and II(all P<0.05).Conclusion TheI-shaped nasal fixation tape combined with a flapping cheek fixation tape in fixing the nasogastric tube can effectively reduce the incidence of unplanned removal of nasogastric tube and meanwhile the patients may feel comfort and satisfactory.