1.A comparative study of the diagnostic value of endoscopic ultrasonography with pathological features of upper gastrointestinal mesenchymal tumors
Bin CHENG ; Li ZHONG ; Fang DING ; Huaping XIE ; Ying WANG ; Yuzhen YANG ; Mei LIU ; Jie WAN
Chinese Journal of Internal Medicine 2009;48(9):724-728
ostic sensitivity and specificity of EUS are high in distinguishing benign and malignant character of upper digestive tract GIMTs. EUS plays an important role in guiding the clinical management of upper digestive tract GIMTs.
2.Energy relieving effect of different thickness of mucosa beneath mandibular complete denture.
Wen-Zhi SONG ; Wan-Zhong YIN ; Mei-Hua LI
Chinese Journal of Stomatology 2004;39(1):57-59
OBJECTIVETo analyze the energy relieving effect of different thickness of mucosa beneath mandibular complete denture and try to analyze clinical question of prosthodontics using energy analysis methods.
METHODSA 3-DFE model of the mandibular complete denture and its supporting tissue were set up. Their elastic deformed energy and the percentage in the whole system were respectively calculated when mucosal thickness was different.
RESULTSThe percentage of mucosal elastic deformed energy grew from 44.53% to 52.91% and 57.91% with its different thickness under static loads.
CONCLUSIONSThe energy relieving effect of mucosa grows with its thickness and the approach of energy analysis is one of effective method on oral biomechanics questions.
Biomechanical Phenomena ; Dental Stress Analysis ; Denture, Complete, Lower ; Elasticity ; Finite Element Analysis ; Humans ; Mouth Mucosa ; anatomy & histology
3.Metabolic changes in abnormal savda patients with different types of tumor: a clinical observation.
Yin CHEN ; Mei-Zuo ZHONG ; Halmurat UPUR ; Batur MAMTIMIN ; Wan-Li DENG ; Rui WANG ; Kamilijiang MAYILA
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(5):549-553
OBJECTIVETo explore in vivo metabolic changes in abnormal savda patients with different types of tumor.
METHODSA total of 142 abnormal savda patients with common cancer types were enrolled in this study, and 50 healthy volunteers were recruited as the control group. For each sample, the H Nuclear Magnetic Resonance (NMR) based metabonomic analysis was performed. The free attenuation signal was computed subsection integral. Data obtained were analyzed by the Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA).
RESULTSCompared with the control group, leucine, isoleucine, valine, histidine, phenylalanine, tyrosine, alanine, creatine, lactic acid, inositol, alpha-and beta-glucose, unsaturated lipids, very low density lipoprotein (VLDL) significantly decreased (P <0.05), while glycoprotein and carnitine significantly increased (P <0. 05) in the abnormal Savda group.
CONCLUSIONAbnormal savda patients with different types of tumor had similar metabonomics changes.
Discriminant Analysis ; Humans ; Least-Squares Analysis ; Lipids ; blood ; Magnetic Resonance Spectroscopy ; Metabolome ; physiology ; Metabolomics ; Neoplasms ; metabolism
4.Comparison of vitrification and slow-freezing of human day 3 cleavage stage embryos:postvitrification development and pregnancy outcomes
Yuan LI ; Zi-Jiang CHEN ; Hui-Jun YANG ; Wan-Xia ZHONG ; Shui-Ying MA ; Mei LI ;
Chinese Journal of Obstetrics and Gynecology 2000;0(11):-
Objective To compare the effects of vitrification with slow-freezing on the developmental ability of day 3 cleavage stage embryos.Methods Patients who had no less than 4 high quality embryos were included in this study.These embryos were cryopreserved using the methods of vitrification or slow-freezing.In the eryopreserved embryo transfer cycles,the embryos which were cryopreserved using one of the methods were chosen randomly.The developmental ability of embryos was compared between these two groups.Results A total of 80 patients were included in this study with 160 embryos.In the group of slow-freezing,73(91%)embryos were survived and achieved 15(38%)clinical pregnancies.Among these,3 were twins and the implantation rate was 25%(18/73).In the group of vitrification,71(89%)embryos were survived and achieved 19(48%)clinical pregnancies.Among these, 9 were twins and the implantation rate was 39%(28/71),which was significantly higher than the slow- freezing group(P
5.Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction.
Mei-lin XU ; Hao-hao ZHONG ; Wan-jie HENG ; Bing-quan WU
Chinese Journal of Pathology 2006;35(12):735-737
OBJECTIVETo study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.
METHODSArchival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.
RESULTSAmong the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.
CONCLUSIONSMycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.
Adult ; DNA, Bacterial ; analysis ; Female ; Fluorescence ; Follow-Up Studies ; Humans ; Lymph Nodes ; microbiology ; pathology ; Male ; Middle Aged ; Mycobacterium tuberculosis ; genetics ; isolation & purification ; Paraffin Embedding ; Polymerase Chain Reaction ; methods ; Sarcoidosis ; microbiology ; pathology
6.Polymorphism of MTHFR C677T gene and MTHFR A1298C gene among physical examinees in Taizhou City
Wan-Hong GU ; Pan WANG ; Qian-Yi ZHONG ; Ying-Mei YANG
Journal of Preventive Medicine 2018;30(4):370-373
Objective To explore the distribution of methylenetetrahydrofolate reductase (MTHFR) C677T gene and MTHFR A1298C gene polymorphism among 1644 physical examinees in Taizhou region. Methods Fluorescence quantitative PCR method was used to detect MTHFR C677T and MTHFR A1298C genotype in 1644 physical examinees from Taizhou Central Hospital from September 2016 to September 2017. According to the characteristics of gender, the distribution features of MTHFR C677T and MTHFR A1298C genotype were described, and then were compared with data about the physical examinees in other cities in China. Results Among the 1644 physical examinees, MTHFR 677CC, 677CT and 677TT genotype frequency were 40.09%, 44.53%and 15.39% respectively and MTHFR 677 allele frequency was 37.65%. MTHFR 1298AA, 1298AC and 1298CC genotype frequency were 65.69%, 30.47% and 3.83% respectively and MTHFR 1298 allele frequency was 19.07%. Statistical significance was found in genotype distribution of MTHFR C677T between males and females (P=0.036), and no statistical significance was found in genotype distribution of MTHFR A1298C between males and females (P=0.278) . As compared with the physical examinees in Henan, Wulumuqi and Beijing regions, there were statistically significance differences in the distribution of MTHFR C677T genotype and allele frequencies in Taizhou region (P <0.05) . Conclusion The distribution of MTHFR C677T gene polymorphism among physical examinees in Taizhou region is affected by gender, and the results shows certain regional specificity.
7.The clinicopathological features of early renal amyloidosis.
Su-xia WANG ; Wan-zhong ZOU ; Mei WANG ; Jie E ; Shu-he WANG ; Xiu-ying TANG
Chinese Journal of Pathology 2003;32(2):120-123
OBJECTIVETo investigate the clinicopathological manifestations of early renal amyloidosis (AL) and its diagnostic criteria.
METHODSFifteen cases with early renal amyloidosis admitted from 1994 to 2001 were collected from the hospital, and their clinical and pathological features were reviewed. Of them, the initial diagnoses were not made by depending findings from the light microscopy (LM) and immunofluorescense (IF), but confirmed by electron microscopy (EM) afterwards. Immuno-electron microscopy (IEM) were applied for amyloidosis typing.
RESULTSMost patients of early renal AL were in the middle to old age. Nephrotic syndrome was the most prominent symptoms and signs accompanying with rare microscopic hematuria and hypertension. Most of them had a normal renal function. Pathological examinations of renal biopsies using LM and IF showed mild mesangial proliferation and mild thickening of glomerular basement membrane (GBM). Immunoglobulins and complements were negative or only scanty in certain cases, but in all cases there was a light chain protein deposition homogeously. There were 4 cases of minimal change glomerulopathy, 5 cases of mild mesangial proliferative glomerulonephritis, 5 cases of stage I membranous nephropathy, and 1 case of cast nephropathy diagnosed with LM. The amyloid fibrils (diameter 8 - 10 nm) were randomly distributed in the mesangium, along GBM and at the arteriolar wall under EM. Additionally, Congo red staining was positive. IEM demonstrated that amyloid fibrils labeled with colloid gold was combined with a kind of light chain protein which was confirmed as the light chain type of AL.
CONCLUSIONSThe diagnosis of early renal AL was occasionally neglected by depending only findings of LM and LF. However, special amyloid fibrils can be detected using EM. EM observation is an indispensable technique for the diagnosis of early renal AL and the typing of AL may further be determined by using IEM.
Adult ; Aged ; Amyloidosis ; metabolism ; pathology ; Basement Membrane ; metabolism ; Female ; Humans ; Immunoglobulin Light Chains ; metabolism ; Kidney Diseases ; metabolism ; pathology ; Kidney Glomerulus ; metabolism ; pathology ; Male ; Microscopy, Immunoelectron ; Middle Aged
8.Schwann cells transplantation promoted and the repair of brain stem injury in rats.
Hong WAN ; Yi-Hua AN ; Mei-Zhen SUN ; Ya-Zhuo ZHANG ; Zhong-Cheng WANG
Biomedical and Environmental Sciences 2003;16(3):212-218
OBJECTIVETo explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats.
METHODSSchwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively.
RESULTSBrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43 expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas.
CONCLUSIONThe transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.
Animals ; Antimetabolites ; Brain Injuries ; therapy ; veterinary ; Brain Stem ; injuries ; Bromodeoxyuridine ; Cell Transplantation ; methods ; veterinary ; Electrophysiology ; Rats ; Rats, Wistar ; Schwann Cells ; transplantation
9.Effect of rat Schwann cell secretion on proliferation and differentiation of human neural stem cells.
Yi-Hua AN ; Hong WAN ; Ze-Shun ZHANG ; Hong-Yun WANG ; Zhi-Xing GAO ; Mei-Zhen SUN ; Zhong-Cheng WANG
Biomedical and Environmental Sciences 2003;16(1):90-94
OBJECTIVETo investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embryonic neural stem cells (NSCs).
METHODSThe samples were divided into three groups. In Group One, NSCs were cultured in DMED/F12 in which Schwann cells had grown for one day. In Group Two, NSCs and Schwann cells were co-cultured. In Group Three, NSCs were cultured in DMEM/F12. The morphology of NSCs was checked and beta-tubulin, GalC, hoechst 33342 and GFAP labellings were detected.
RESULTSIn Group One, all neural spheres were attached to the bottom and differentiated. The majority of them were beta-tubulin positive while a few of cells were GFAP or GalC positive. In Group Two, neural spheres remained undifferentiated and their proliferation was inhibited in places where Schwann cells were robust. In places where there were few Schwann cells, NSCs performed in a similar manner as in Group One. In Group Three, the cell growth state deteriorated day after day. On the 7th day, most NSCs died.
CONCLUSIONThe secretion of rat Schwann cells has a growth supportive and differentiation-inducing effect on human NSCs.
Animals ; Brain ; cytology ; embryology ; Cell Differentiation ; drug effects ; Cell Division ; drug effects ; Coculture Techniques ; Humans ; Rats ; Rats, Sprague-Dawley ; Schwann Cells ; secretion ; Sciatic Nerve ; cytology ; Stem Cells ; cytology
10.Surgical treatment for posterior hip dislocations complicated with fractures of femoral head and acetabulum.
Zhong-mei WAN ; Yi-ping ZHANG ; Ming-gao SHEN ; Jun-de XIAO
China Journal of Orthopaedics and Traumatology 2008;21(9):672-673
OBJECTIVETo explore the effectiveness and prognosis on surgical treatment of posterior hip dislocations complicated with fractures of femoral head and acetabulum.
METHODSSeventeen patients with posterior hip dislocations complicated with fractures of femoral head and acetabulum were reviewed in the study. All the patients were treated with manual reduction within 12 hours after injury. CT location was used before operation. Absorbable screw fixation was performed for the femoral head fractures, while plate fixation or resection was performed for acetabular fractures.
RESULTSAll the patients got bony union within 6 months after operation without femoral head necrosis. Evaluate joint function according to Modifie Daobigne and Postal clinical classification criteria, 8 patients got an excellent result, 7 good and 2 fair. The excellent and good rate was 88.2%.
CONCLUSIONSurgical treatment can have a satisfactory prognosis for patient with posterior femoral head and acetabulum.
Acetabulum ; injuries ; Adolescent ; Adult ; Female ; Femoral Fractures ; complications ; Follow-Up Studies ; Hip Dislocation ; complications ; physiopathology ; surgery ; therapy ; Humans ; Male ; Middle Aged ; Treatment Outcome