Objective To investigate the oxidative stress,microinflammation and their relationship in patients with MHD(maintenance hemodialysis)and to evaluate the effect and safety of vitamin E supplementation in patients with MHD.Methods Fifty-eight patients with MHD and 20 healthy volunteers were involved in this study.The patients were administered 400 mg/d of vitamin E for 6 weeks.The levels of oxidative vitamin E(Vit E),malondialdehyde(MDA),advanced oxidation protein product(AOPP),superoxide dismutase(SOD),activity of glutathione peroxidase(GSH-PX),microinflammation cytokines C-reactive protein(CRP),interleukin-6(IL-6),interleukin-10(IL-10) and tumor necrosis factor(TNF-?)in plasma were measured at the beginning and the end of administering Vit E.Results 400 mg/d of Vit E supplementation decreased the levels of plasma AOPP,MDA,CRP,TNF-?(P

Objective To investigate the influence of long-term blood purification on atherosclerosis(AS) in maintenance hemodialysis(MHD) patients by measuring serum levels of homocysteine(Hcy) and carotid artery intima-medial thickness(IMT).Methods Seventy-two MHD patients visiting our hospital from June 2006 to December 2007,according to their blood purification mode,were assigned to three groups: hemodialysis(HD,n=25),hemodialysis+hemodiafiltration(HD+HDF,n=30),hemodialysis+continuous renal replacement therapy(HD+CRRT,n=17).Another 25 healthy subjects served as controls.Serum Hcy was detected before and after one-year treatment and meanwhile color Doppler ultrasonography was performed to check IMT of carotid artery and atheromatous plaques.Results The serum levels of Hcy,IL-6,MCP-1,hs-CRP in MHD patients were significantly higher than those in healthy controls(P

Objective To determine the normal ranges of N-terminal pro-brain natriuretic peptide(NT-proBNP) in normal children and children with symptoms of heart failure(HF),and to study their clinical implications.Methods Concentrations of serum NT-proBNP were determined in 80 normal children and 70 children with clinical symptoms of HF.Venous blood was taken in each subject,and plasma NT-proBNP was determined by ELISA method.Eighty normal children included 40 boys and 40 girls.Their ages ranged from 1 to 16 years old.Seventy children with HF were divided into two groups.The first group(n=45,21 males,24 females) included children with symptoms of HF caused by dilated cardiomyopathy(DCM).Their ages ranged from 1 to 16 years,and they had a left ventricular ejection fraction(LVEF) of less than 50%.The second group(n=25,11 male,14 female) consisted of children with symptoms of HF due to ventricular septal defect(VSD).Their ages ranged from 1 to 16 years,and they had an LVEF of 51%-78%.The serum NT-pro BNP levels were determined by ELISA method and LVEF was measured by echocardiography and clinical symptom score of heart failure was defined by using Ross Score.Results Serum concentration of NT-proBNP was 223.05 fmol/mL in normal children from 1 to 16 years old.NT-proBNP levels did not show a significant age-related or sex-related differences.In children with HF,the plasma NT-proBNP levels were significantly elevated(mean:1353.3 fmol/mL) compared to normal children(t=8.964 P

Objective To estimate the impact of cold wave on daily death of cerebra-cardiovascular diseases in Beijing by using case-crossover design. Methods The data of death counts was provided by Beijing Center for Disease Control and Prevention while the meteorological data was provided by Institute of Urban Meteorology,China Meteorological Administration (CMA),Beijing. The impact of six cold waves from Jan 1,1998 to Jun 30,2000 on daily death of cerebra-cardiovascular diseases were analyzed by using 1∶1 one-directional retrospective design and 1∶2 bi-directional symmetry control design. The cases were the people who dead during winter. Controls were the seventh day before or after the date of cases (or the fourteenth day). Results There were six cold waves in the investigation duration. The third cold wave in which minimum temperature declined obviously with decreasing humidity and increasing air pressure had significantly statistical significance in OR and the lag time of this cold wave was 0 day,the OR values were 1.500 (95%CI:1.032-2.181) for cardiovascular disease (CVD),respectively 1.913 (95%CI:1.066-3.432) for acute myocardial infarction,1.679 (95%CI:1.139-2.474) for cerebra-vascular disease(CBD) respectively. The other five cold waves do not have significant impact on cerebra-cardiovascular diseases. Conclusion Most cold waves studied in Beijing do not impact the daily death counts caused by cerebra-cardiovascular diseases. The cold wave with significant increased air pressure may increase the risk of death of cerebra-cardiovascular diseases without any lag time in Beijing.

Gastrointestinal bleeding is a common complication of gastrointestinal diseases and gastrointestinal surgery, which may lead to hemorrhagic shock or cause death if not treated properly and promptly.Currently, endoscopic treatment for gastrointestinal bleeding includes clip closure, endoscopical injection of saline/adrenaline, argon plasma coagulation (APC), electrocoagulation and heater probe coagulation etc..Endoscopic clip closure mainly includes traditional closure through-the-scope clip (TTSC) and more novel closure over-the-scope clip (OTSC).This article reviewed the use of OTSC in the treatment of gastrointestinal bleeding.

Applying problem-based learning(PBL)teaching method to clinical teaching of nephrology.Questionnaire were used to evaluate the teaching effect.The conclusion is that PBL method can enhance students'ability of active learning,analyzing and solving problems

Diagnostic Errors ; Electrocardiography ; Humans ; Inferior Wall Myocardial Infarction ; Meningeal Neoplasms ; Meningioma ; Myocardial Infarction ; diagnosis

Objective To analyze the adverse drug reaction of intravitreal conbercept injection for the security of conbercept injection in clinical application.Methods From 2014 to 2016,248 cases of adverse drug reaction caused by intravitreal conbercept injection were monitored by 62 medical institutions from National Center for Adverse Drug Reaction Monitoring China,and analyzed in this study.Results The adverse drug reactions caused by conbercept were mainly observed in the ophthalmology system,skin system,musculoskeletal system and cardiovascular system.The adverse drug reactions mainly occurred in the early stage of injection.Among those patients,the reported treatment results were cured in 126 cases,improved in 120 cases,unchanged in 1 case,unknown in 1 case,and no anyone died.Conclusion It should give a great attention to the serious adverse reaction that caused by conbercept injection,especially for endophthalmitis,retinal detachment and vitreous hemorrhage.The incidence of serious and newly observed adverse drug reaction should be two important portions of post-marketing safety surveillance.Moreover,the quality of adverse drug reaction reports from different medical institutions needs to be improved.Their assessment and management should be enhanced.

Objective To investigate the effect of brain-derived neurotrophic factor on protection of rat hippocampal neurons after cultured with high glucose concentrations.Methods Hippocampal neurons of neonatal SD rat were cultured in serum-free medium and divided into normal group,high glucose group and high glucose BDNF group.The morphological changes of the neurons were observed under inverted fluorescence microscope.Immunofluorescence stain for Map2 was performed to identify the purity of neurons.The survival of the neuron were tested by PI/Hoechest33342 staining.Results Compared with the normal group [survival rate (96.11 ± 1.63) ％],the number of PI positive neurons were significantly increased in the high glucose group [survival rate (76.29 ± 6.74) ％].Treatment with BDNF [survival rate (93.47 ±2.43) ％] significantly reduced the number of PI positive cells.Conclusion BDNF can protect rat hippocampal neurons from apoptosis induced by high glucose.

Objective To investigate the clinical treatment efficacy of mutabilis coated nasal ointment to postoperative nasal septal reconstruction.Methods After surgical reconstruction,116 cases were divided into the treatment group and control group ac-cording to random method,58 cases in each group.The treatment group was given mutabilis coated nasal ointment combine with physiological seawater nasal care device;the control group was only given physiological seawater nasal care device in the same time. The effects were observed for 6 months.Results The total effective rate of treatment group was higher than the control group (98.28%),the two groups had significant difference(P <0.05).Postoperative follow-up,the bleeding rate in the treatment group have no significant difference compared with the control group(P >0.05);the incidence of infection and cavity stricture in the treat-ment group were significantly lower than the control group(P <0.05).Conclusion The application of mutabilis coated nasal oint-ment can promote the wound healing of nasal cavity,but also can prevent the operation adhesion,and shorten repair time,so as to improve the clinical effect of operation.